Sphingobacterium prati sp. nov., isolated from agricultural soil and involved in lignocellulose deconstruction Author Besaury, Ludovic Author Floret, Juliette Author Rémond, Caroline text International Journal of Systematic and Evolutionary Microbiology 2021 004963 2021-08-18 71 8 1 8 http://dx.doi.org/10.1099/ijsem.0.004963 journal article 10.1099/ijsem.0.004963 9ac3ec99-58c9-4ee6-827f-295b3821ff0c 1466-5034 PMC8513620 34406922 6224196 DESCRIPTION OF SPHINGOBACTERIUM PRATI SP. NOV. Sphingobacterium prati (pra'ti L. gen. n. prati of a meadow). Cells are Gram-stain-negative, non-motile, non-sporeforming, strictly aerobic rods, 0.8–1.7 µm long and 0.7–0.9 µm wide. After 2 days incubation on LB agar, colonies are 1.0– 2.5 mm in diameter, slightly yellowish, convex, circular and smooth. Growth occurs at 15–37 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 0–4% NaCl (optimum, 0 %). Growth occurs on nutrient agar and LB agar, but not on MacConkey agar. Positive for catalase and oxidase. Acid is produced from sodium lactate, acetoacetic acid, cellobiose, dextrin, D-fructose,D-galactose, D-galacturonic acid,D-gluconic acid, maltose, D-mannose, melibiose, D-salicin, turanose, gelatin, gentiobiose, glycyl-L-proline, guanidine HCl, L-fucose, L-rhamnose, N -acetyl-D-galactosamine, N -acetyl-β-D-mannosamine, nalidixic acid, pectin, sodium butyrate, sucrose, D-glucose and lactose. Acid is not produced from 3-methyl-glucose, bromo-succinic acid, citric acid, D-arabitol,D-aspartic acid, D-fructose-6- PO 4 , D-fucose,D-glucose-6- PO 4 , D-gluronicacid, D-lactic acid, methyl ester, D-malic acid,D-mannitol, raffinose, D-saccharic acid,D-serine,D-sorbitol, trehalose, formic acid, fusidic acid, glucuronamide, glycerol, inosine, L-alanine, L-arginine, L-aspartic acid, L-galactonic acid, lactone, L-glutamic acid,L-histidine,L-lactic acid,L-malic acid, L-pyroglutamic acid,L-serine, methyl pyruvate, mucic acid, myo-anositol, N -acetyl neuraminic acid, N -acetyl-Dglucosamine, p -hydroxy-phenylacetic acid, propionic acid, quinic acid, stachyose, Tween 40, α-hydroxy-butyric acid, α-keto-butyric acid, α-keto-glutaric acid, β-hydroxy-D acid, L-butyric acid, methyl β-D-glucoside and γ-amino-butyric acid. Resistant to aztreonam, rifamycin and sensitive to lincomycin, minocycline, troleandomycin and vancomycin. Strain arapr2 T harbours β-galactosidase but not lysine decarboxylase, ornithine decarboxylase, urease and tryptophane desaminase ( API 10 S). Strain arapr2 T can oxidize glucose and produce NO2 but cannot oxidize arabinose or produce H 2 S ( API 10 S). The predominant fatty acids are iso-C 15:0 , C 16:0 and C 17:0 iso 3-OH. Menaquinone-7 is the major respiratory quinone. The major polar lipids are glycolipid, aminolipid, phospholipid, phosphatidylethanolamine, phosphoaminolipid and phosphoaminoglycolipid. The type strain, arapr2 T (CIP 111872 T =LMG 31848 T ), was isolated from meadow soil collected near Reims , France . The DNA G+C content of the type strain is 40.4 mol%.