Scratching the tip of the iceberg: integrative taxonomy reveals 30 new species records of Microgastrinae (Braconidae) parasitoid wasps for Germany, including new Holarctic distributions Author Hoecherl, Amelie https://orcid.org/0009-0007-4211-7468 SNSB-Zoologische Staatssammlung Muenchen, Muenchhausenstr. 21, 81247 Muenchen, Germany amelie.hoecherl@gmail.com Author Shaw, Mark R. https://orcid.org/0000-0002-6651-8801 National Museums of Scotland, Chambers Street, Edinburgh EH 1 1 JF, UK Author Boudreault, Caroline https://orcid.org/0000-0002-4511-2626 Canadian National Collection of Insects, Arachnids and Nematodes, 960 Carling Ave., Ottawa, K 1 A 0 C 6, Canada Author Rabl, Dominik https://orcid.org/0000-0002-0613-7804 Field Station Fabrikschleichach, Department of Animal Ecology and Tropical Biology, Biocenter, University of Wuerzburg, Glashuettenstr. 5, Wuerzburg, 96181 Rauhenebrach, Germany Author Haszprunar, Gerhard Department Biology II, Ludwig-Maximilians-Universitaet Muenchen (LMU), Grosshaderner Str. 2, Martinsried, 82152 Planegg, Germany Author Raupach, Michael J. https://orcid.org/0000-0001-8299-6697 SNSB-Zoologische Staatssammlung Muenchen, Muenchhausenstr. 21, 81247 Muenchen, Germany Author Schmidt, Stefan https://orcid.org/0000-0001-5751-8706 SNSB-Zoologische Staatssammlung Muenchen, Muenchhausenstr. 21, 81247 Muenchen, Germany Author Baranov, Viktor https://orcid.org/0000-0003-1893-3215 Estacion Biologica de Donana-CSIC / Donana Biological Station-CSIC, Seville, Spain Author Fernandez-Triana, Jose https://orcid.org/0000-0003-0425-0309 Canadian National Collection of Insects, Arachnids and Nematodes, 960 Carling Ave., Ottawa, K 1 A 0 C 6, Canada text ZooKeys 2024 2024-01-11 1188 305 386 http://dx.doi.org/10.3897/zookeys.1188.112516 journal article http://dx.doi.org/10.3897/zookeys.1188.112516 1313-2970-1188-305 CBA8C74195AB4DB59E80AAAA500D3572 C4B2907AEF025898B974548D1D87BC3B Microplitis naenia Nixon, 1970 Material examined. Czech Republic : South Moravia : Obora Soutok , Lanzhot , 48.69, 16.945, 165 m , ex. Orthosia cruda , 14.v.2013 , leg. P. Drozd , BC- ZSM-HYM-23873-E06 ; Germany : Bavaria : Grettstadt , 49.963 , 10.372 , 248 m , canopy fogging, 28.v.2021 , leg. B. Leroy , ZSM-HYM-42393-D05; Iphofen, 49.646 , 10.315 , 355 m , canopy fogging, 2.v.2019 , leg. B. Leroy , ZSM-HYM-33158-A10; Prichsenstadt, 49.855 , 10.304 , 258 m , canopy fogging, 1.v.2019 , leg. B. Leroy , ZSM-HYM-33158-A09; Schonungen, 50.077 , 10.429 , 359 m , canopy fogging, 23.v.2019 , leg. B. Leroy , ZSM-HYM-33158-E05; ZSM-HYM-33158-E06; Wiesentheid, 49.803 , 10.277 , 216 m , canopy fogging, 1.v.2019 , leg. B. Leroy , ZSM-HYM-33158-B02; ZSM-HYM-33158-B03 ; Thueringen : Neubrunn , 50.511 , 10.457 , 448 m , canopy fogging, 29.v.2021 , leg. D. Rabl , ZSM-HYM-42447-D08 . Geographical distribution. PAL. PAL- Czech Republic*, Germany*, Hungary, Russia (C, NW), Slovakia, Turkey, United Kingdom. Molecular data. BINs: BOLD:ABV9098, BOLD:AEK2564. Host information. Host of type unknown; also Noctuidae : Cosmia trapezina (Linnaeus, 1758), Conistra vaccinii (Linnaeus, 1761), Eupsilia transversa (Hufnagel, 1766), Orthosia cruda (Denis & Schiffermueller , 1775), Orthosia cerasi (Fabricius, 1775), Rileyiana fovea (Treitschke, 1825). Notes. The morphology of our material (see Fig. 41 ) matches the species described in Nixon (1970) , as well as the keys in Papp (1984b) , Tobias (1986) , and Kotenko (2007) . There are two BINs which we associate with this species. Our specimens show some intraspecific morphological variability, which matches what Nixon mentioned in the original description of the species. Specimens of both barcoding clusters parasitise the same host, Orthosia cruda , from which two of Nixon's paratypes were reared. One specimen from BOLD:ABV9098 (BC- ZSM-HYM-23873-E06) was reared from this species, and we have metabarcoding data that strongly indicates that members of BOLD:AEK2564 parasitise the same host species. Metabarcoding of 22 individual caterpillars of O. cruda resulted in more than 100 reads per caterpillar of sequences that match this BIN. With this information, we consider our specimens of both barcoding clusters to represent members of M. naenia . BIN BOLD:ABV9098 and BOLD:AEK2564 are separated by 1.71% p-distance and the species has an intraspecific variability of 2.63% (p-distance). The hosts associated with this wasp species were reported either by Nixon (1970) , as part of the original description, or by Capek (1972) and Capek et al. (1982) ; we consider both to be reliable because some of the material reported by Nixon (1970) came from Capek. This species is illustrated in Fig. 41 . Figure 41. Microplitis naenia Nixon, 1970, female (ZSM-HYM-42447-D08) A lateral view B meso- and metasoma C wing. Length of the specimen: 3.5 mm.