Geographic variation in the calling songs and genetics of Bartram's round-winged katydid Amblycorypha bartrami (Tettigoniidae, Phaneropterinae) reveal new species Author Forrest, Timothy G. Department of Biology, University of North Carolina at Asheville, Asheville, NC, USA. tforrest@unca.edu Author Scobie, Micaela Department of Biology, University of North Carolina at Asheville, Asheville, NC, USA. Author Brueckner, Olivia Department of Biology, University of North Carolina at Asheville, Asheville, NC, USA. Author Bintz, Brittania Department of Chemistry and Physics, Western Carolina University, Cullowhee, NC, USA. Author Spooner, John D. Department of Biology, University of South Carolina at Aiken, Aiken, SC, USA. text Journal of Orthoptera Research 2023 2023-09-21 32 2 153 170 http://dx.doi.org/10.3897/jor.32.96295 journal article http://dx.doi.org/10.3897/jor.32.96295 1937-2426-2-153 E6535B4D60EE4FF682D9E2899E64FCE7 485235E05BD65285A0F4F6D1FA42A146 Amblycorypha peedee Forrest sp. nov. Figs 5 , 7 -15 , 17 Material examined. - Holotype : USA • ♂ ; North Carolina , Richmond Co. , Sandhills Gamelands ; 35.0528°N , 79.6035°W ; 1 Jul. 2006 ; T.G. Forrest leg.; Ambar ?-M03-2006; DNA (MS-044) NCBI accession SAMN31929325; REC (2006 Tape01 PGM 04); UNCA to be transferred to FSCA. Allotype : USA • ♀ ; North Carolina , Richmond Co. , Sandhills Gamelands ; 35.06139°N , 79.63982°W ; 16 Jul. 2004 ; T.G. Forrest leg.; Ambar ?-F01-2004; DNA (NA); REC (NA); UNCA to be transferred to FSCA. Paratypes : ( 8♂ , 0 ♀ ) USA • 1♂ ; North Carolina , Richmond Co. ; 16 Jul. 2004 ; T.G. Forrest leg.; UNCA to be transferred to FSCA • 6♂ ; North Carolina : Richmond Co. ; 1 Jul. 2006 ; T.G. Forrest leg.; UNCA to be transferred to FSCA • 1♂ ; North Carolina , Richmond Co. ; 19 Jul. 2007 ; T.G. Forrest leg.; UNCA to be transferred to FSCA . Other specimens :- 2♂, 0♀: North Carolina specimens from Walker et al. 2003 deposited in Florida State Collection of Arthropods. USA • 1♂; North Carolina, Moore Co.; T.J. Walker leg.; (doublet song); FSCA • 1♂; North Carolina, Hoke Co; 26 Jul. 1964; T.J. Walker leg.; FSCA. Size measurements (mm). - Holotype: PrnL: 5.9, PrnW: 4.45, TegL: 27.8, TegW: 10.0, HwEx: 3.9, FemL: 27.2, TibL: 28.8mm ( Fig. 17 ). Allotype: PrnL: 7.0, PrnW: 46, TegL: 28.0, TegW: 9.6, HwEx: 2.2, FemL: 29.9, TibL: 31.0, OviL: 11.0mm. Fig. 17. Holotype of Amblycorypha peedee , PeeDee round-winged katydid. A. Dorsal view; B. Lateral view. Scale bars: 5 mm. Etymology. - This species is named for its geographic location, with populations north of the Pee Dee River, which isolates it from populations of A. carolina . Common name. - Pee Dee round-winged katydid. Differential diagnosis. - Amblycorypha peedee is best distinguished from other species in the Amblycorypha rotundifolia complex by calling song. Syllables are almost always produced in doublets with syllable rates within doublets of about 11.6s-1 at 25°C ( Fig. 8 ) and rates of 4.0s-1 at 25°C between doublets. The sustained portion of calling songs of A. peedee has about 17 syllables with 2-3 syllables in each of the series preceding the sustained portion ( Fig. 7 ). Description. - Individuals are typically green with characteristics of the Amblycorypha rotundifolia complex of Amblycorypha ( Walker et al. 2003 ). The male's calling song consists of a single syllable type that are produced in several (4-12) bouts of a 2-3 syllables preceding a sustained series of about 17 syllables ( Figs 5 , 7 ) that occur in pairs (doublets) ( Fig. 5D ). During the sustained portion of the song, at 25°C the syllable rate between doublets is about 4.0s-1 and is about 11.6s-1 within the doublet. The function of syllable rate within a doublet in response to changes in temperature is rate=0.583(temp)-2.92 ( Fig. 8 ). Female size measurements for the single individual that was collected are PrnL: 7.05; PrnW: 4.58; TegL: 28.0; TegW: 9.63; FemL: 29.9; TibL: 31.0; OviL: 11.0. Males' measurements ( x -̄ +/- SE in mm, N) are on average PrnL: 5.84 +/- 0.04, 9; PrnW: 4.32 +/- 0.06, 9; TegL: 28.2 +/- 0.21, 9; TegW: 9.32 +/- 0.18, 9; FemL: 27.3 +/- 0.38, 6; TibL: 28.7 +/- 0.44, 6 (Table 2 ).