A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene paulista Brölemann, 1902 Figs 25A, C, E , 32C , 109–110 , 164G , 166F , 171D , 178I , 186 ; Supp. file 4: Figs 199B , 201B , 212D , 213B , 218E Pseudonannolene paulista Brölemann, 1902a: 129 , pl. vi figs 142–147. Pseudonannolene paulista – Jeekel 2004: 90 . — Iniesta & Ferreira 2013a: 92 . — Gallo & Bichuette 2020: 36 . Diagnosis Males of P. paulista slightly resemble those of P. aurea sp. nov. by having a solenomere with a spiniform ectal process deeply notched separating it from the apicomesal process ( Fig. 110D ), but differing by an internal branch shovel-shaped with horizontal plate ( Fig. 110D–F ). Etymology Although unspecified, the name is evidently related to the demonym “paulista”, in reference to the inhabitants of the Brazilian state of São Paulo , where the species occurs. Material examined Paralectotypes (total: 1 ♂ , 1 ♀ ) BRAZIL • 1 ♂ , 1 ♀ ; São Paulo , Cerqueira César ; [ -23.067354 , -49.157619 ]; Dec. 1896 ; R. von Ihering leg.; MZSP . Other material (total: 20 ♂♂ , 37 ♀♀ , 1 immature ) BRAZIL – São Paulo • 1 ♀ ; Assis , Estação Ecológica de Assis ; [ -22.661071 , -50.419104 ]; 576 m a.s.l. ; 25–30 Nov. 2002 ; Equipe Biota leg.; IBSP 7896 • 2 ♀♀ ; same collection data as for preceding; IBSP 2972 • 1 ♂ , 1 ♀ ; same collection data as for preceding; IBSP 7894 • 2 ♂♂ ; same collection data as for preceding; 20 Oct. 2002 ; Equipe Biota leg.; IBSP 2982 • 1 ♂ ; Angatuba , Estação Ecológica de Angatuba ; [ -23.415855 , -48.360834 ]; 761 m a.s.l. ; 11–16 Nov. 2002 ; Equipe Biota leg.; IBSP 1908 • 2 ♀♀ ; same collection data as for preceding; IBSP 1915 • 1 ♀ ; same collection data as for preceding; IBSP 1912 • 1 ♂ , 1 ♀ ; Piracicaba , Escola Superior de Agricultura Luiz de Queiroz (ESALQ-USP); [ -22.711635 , -47.627783 ]; 548 m a.s.l. ; 11–31 Mar. 1994 ; A. Eterovic leg.; IBSP 1261 • 1 ♂ , 1 ♀ ; same collection data as for preceding; IBSP 1261 • 1 ♂ , 15 ♀♀ , 1 immature ; Anhembi , Fazenda Barreiro Rico ; [ -22.788342 , -48.131224 ]; 469 m a.s.l. ; C. Fontanetti leg.; MZSP • 11 ♂♂ , 11 ♀♀ ; same collection data as for preceding; Dec. 1896 ; A. Mesa leg.; MZSP • 1 ♂ , 2 ♀♀ ; Teodoro Sampaio , Parque Estadual Morro do Diabo ; [ -22.524723 , -52.298448 ]; 367 m a.s.l. ; 24–31 Mar. 2003 ; Equipe Biota leg.; IBSP 2409 • 1 ♂ ; same collection data as for preceding; IBSP 2424 . Descriptive notes MEASUREMENTS . 55–71 body rings (1–2 apodous + telson). Males: body length 67.9–79.4 mm ; maximum midbody diameter 3.2–3.6 mm . Females: body length 76.4–87 mm ; maximum midbody diameter 4.1– 4.9 mm . COLOR . Body color brownish yellow; head, collum, and antennae darker; prozonites and metazonites anteriorly darker, with a posterior band lighter; legs brownish. HEAD . Antennae short ( Fig. 164G ), just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1<2≈3>4=5≈6>7. Mandibular cardo with ventral margin swollen. Ommatidial cluster well-developed, elliptical; ca 40 ommatidia in 5 rows. BODY RINGS . Collum with lateral lobes broadly rounded, with ca 6 striae ( Fig. 109A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae below ozopore. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 171D ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) elongated (as long as the sum of remaining podomere lengths), subtriangular, with the base slightly arched and expanded, densely setose ( Fig. 110A ); prefemoral process ( prf ) as wide as half of prefemur, subcylindrical, densely setose along in its entire extension ( Fig. 110B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) large and subrectangular; penis ( pn ) located at proximal region, rounded, not extended basally ( Fig. 110C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, almost twice as long as telopodite, with the base arched; antero-posteriorly flattened ( Figs 110D–F , 212D , 213B ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder absent. Telopodite ( tp ) almost as wide as gcx ( Figs 110D , 218E ); solenomere ( sl ) with apicomesal process ( amp ) subtriangular; ectal process ( ep ) spiniform, elongated, separating from amp by deep notch; sa located at mesal portion, curved mesad, elongated. Internal branch ( ib ) shovel-shaped, with horizontal plate; long setae restricted to the apical region of ib exceeding seminal region of sl ( Figs 110D–F , 213B ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178I ); internal valve subtriangular, with mesal region rounded; operculum narrow, S-shaped; external valve wide, subtriangular. Distribution Known from the central-west region of São Paulo State , Brazil ( Fig. 186 ); occurring in the Cerrado biome (tropical savanna ecoregion) and in second-growth forests in the region. Comments The lectotype from Cerqueira César and the paralectotypes ( two females ) from Batista Botelho, deposited at the Muséum national d’histoire naturelle, Paris, France (MNHN), were not examined during this study.