A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene occidentalis Schubart, 1958 Figs 19B , 98–100 , 164D , 171A , 178E , 185 ; Supp. file 4: Figs 194B , 199D , 208C , 212A , 216E , 222E Pseudonannolene occidentalis Schubart, 1958: 214 , figs 12–13. Pseudoannolene [sic!] occidentalis – Krabbe 1982: 71 . Pseudonannolene occidentalis – Jeekel 2004: 89 . — Golovatch et al. 2005: 279 . Diagnosis Males of P. occidentalis differ from all congeners by having the frontal region of the head densely setose, overlapping the supralabral and labral setae ( Fig. 19B ); mandibular cardo finely granular, with swollen ventral margin ( Figs 171A , 199D ); solenomere with seminal apophysis located ectally ( Fig. 99D ). Etymology Adjective referring to the geographical distribution of the species in western Brazil ( Schubart 1958 ). Material examined Holotype BRAZIL • ♂ [gonopods, first and second leg-pair on microscope slides]; Mato Grosso do Sul , Salobra ; [ -20.187516 , -56.547016 ]; 112 m a.s.l. ; 22 Jan. 1955 ; L. Travassos leg.; MZSP . Paratypes (total: 2 ♀♀ ) BRAZIL • 2 ♀♀ ; same collection data as for holotype; MZSP . Other material (total: 9 ♂♂ , 7 ♀♀ , 4 immatures ) BRAZIL – Mato Grosso • 2 ♂♂ ; Chapada dos Guimarães , close to Aldeia Velha ; [ -15.464565 , -55.760228 ]; 823 m a.s.l. ; 6 Mar. 2014 ; A. Chagas-Jr and M. Karam-Gemael leg.; CZUFMT 808 • 2 ♂♂ , 1 ♀ , 4 immatures ; Chapada Aventura ; [ -15.464283 , -55.759722 ]; 820 m a.s.l. ; 7 Nov. 2015 ; A. Chagas-Jr et al. leg.; CZUFMT 818 • 1 ♂ , 4 ♀♀ ; same locality data as for preceding; 28 Aug. 2014 ; A. Chagas-Jr leg.; CZUFMT 823 . – Mato Grosso do Sul • 1 ♂ ; Salobra ; [ -20.189192 , -56.547513 ]; 112 m a.s.l. ; 19 Jan. 1941 ; F. Lane leg.; MZSP . – São Paulo • 3 ♂♂ , 1 ♀ ; Jundiaí , Serra do Japi ; [ -23.226630 , -46.924751 ]; 871 m a.s.l. ; 6–10 Aug. 2001 ; Equipe Biota leg.; IBSP 1998 • 1 ♀ ; Jundiaí , Reserva Natural Municipal da Serra do Japi ; [ -23.236337 , -46.943607 ]; 1069 m a.s.l. ; Mar. 2007 ; A.D. Brescovit leg.; IBSP 3100 . Descriptive notes MEASUREMENTS . 70–73 body rings (2 apodous + telson). Males: body length 70–80 mm ; maximum midbody diameter 4.4–5 mm . Females: body length 70 mm ; maximum midbody diameter 4.8 mm . COLOR . Body color brownish yellow; head, collum, and antennae darker; prozonites and metazonites anteriorly darker, with a posterior band reddish; legs brownish. HEAD . Antennae short ( Fig. 164D ), just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1≈2≈3>4=5=6>7. Frontal setae overlapping supralabral and labral ones ( Fig. 19B ). Mandibular cardo with ventral margin swollen ( Figs 171A , 199D ). Ommatidial cluster well-developed, elliptical; ca 25 ommatidia in 4 rows. BODY RINGS . Collum with lateral lobes rounded, with ca 7 striae, slightly curved ectad ( Fig. 98A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae up to ozopore in anterior body rings. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 171A ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) elongated (as long as the sum of remaining podomere lengths), subrectangular, with the base arched, densely setose ( Figs 99A , 100B ); prefemoral process ( prf ) elongated and as wide as half of prefemur, subcylindrical, densely setose along the entire ventral region ( Fig. 99B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) large and subrectangular; penis ( pn ) located at proximal region, rounded, not extended basally ( Figs 99C , 100E–F , 208C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) subtriangular, basally expanded and progressively less wide, with the base slightly arched; antero-posteriorly strongly flattened, longitudinal thickened ridge with rows of papillae mesally ( Figs 99D–F , 100C–D ). Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder absent. Telopodite ( tp ) arising from gcx by short, compressed trunk ( Figs 99D , 100D ); solenomere ( sl ) with apicomesal process ( amp ) short; ectal process ( ep ) short, subtriangular, separating from amp by shallow notch; sa located at ectal portion, elongated and thickened apically. Internal branch ( ib ) shovel-shaped and rounded apically, with horizontal plate; setae restricted to the apical region of ib exceeding seminal region of sl ( Figs 99D–F , 100C–D ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178E ); internal valve subtriangular; operculum large, slightly expanded apically; external valve wide, subtriangular. Distribution Known from the west region of São Paulo up to the states of Mato Grosso do Sul and Mato Grosso , Brazil ( Fig. 185 ); the distribution range of P. occidentalis partially covers the biomes Atlantic Forest, Cerrado, and Pantanal.