Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade
Author
Chen, Kai
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & University of Chinese Academy of Sciences, Beijing 100049, China
Author
Zhuang, Wen-Ying
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & University of Chinese Academy of Sciences, Beijing 100049, China
text
Phytotaxa
2017
2017-07-04
312
1
28
46
http://dx.doi.org/10.11646/phytotaxa.312.1.2
journal article
10.11646/phytotaxa.312.1.2
1179-3163
13701723
Trichoderma shaoguanicum
K. Chen & W.Y. Zhuang
,
sp. nov.
Fig 6
Fungal Names: FN570280.
Etymology
: The specific epithet refers to the
type
locality.
Diagnosis
. Colony growing fast on CMD, PDA and SNA at 25 °C. Conidiophores verticillium-like, phialides subulate, less commonly lageniform or ampulliform, conidia green, ellipsoid.
On CMD after 72 h colony radius
53–55 mm
and mycelium covering the plate after 4 d at 25 °C. Colony hyaline, radial, zonate, aerial hyphae common. Conidiation noted after 4 d, formed on aerial hyphae and in pustules, pustules appearing in several concentric zones, denser along the colony margin, loose, first white, turning green after 6 d. Conidiophores verticillium-like, comprising a recognizable main axis, simple side branches arising along the length of the main axis, mostly paired, sometimes solitary. Phialides paired or in whorls of 3, typically straight, subulate, less commonly lageniform or ampulliform, (6.9–)10.3–15.0(–17.2) × 2.8–4.4 μm, l/w (1.8–)3.0–5.6, 2.1–3.3 μm wide at the base (n = 40). Conidia green, with 1–2 larger or many minute guttules, ellipsoid, 4.4–5.3(–7.2) × 3.6–3.9(–4.7) μm, l/
w 1.2
–1.6 (n = 40). No chlamydospores observed. No distinct odor, no diffusing pigment observed.
On PDA after 72 h colony radius
45–48 mm
and mycelium covering the plate after 5 d at 25 °C. Colony similar to CMD, but with more pustules, pustules spreading in concentric rings, loose, aggregated. Conidiation noted after 2 d, formed on aerial hyphae and pustules. Chlamydospores common. No distinct odor, no diffusing pigment observed.
On SNA after 72 h colony radius
18–20 mm
and mycelium covering the plate after 15 d at 25 °C. Colony hyaline, radial, aerial hyphae inconspicuous, margin not well defined. Conidiation noted after 3 d, formed on pustules, pustules scattered, loose, with irregular shape, first white, turning green after 4 d. Chlamydospores rare. No distinct odor, no diffusing pigment observed.
Strains examined
:
CHINA
.
Guangdong
: Shaoguan, Chebaling, elev.
500 m
, isolated from soil,
9 November 2015
,
K. Chen TC826
(
holotype
HMAS 248809, ex-type culture CGMCC 3.18323).
ibid
.,
K. Chen TC825
(HMAS 248808).
Notes
: According to our phylogenetic analyses (
Fig 1
),
T. shaoguanicum
is closely related to
T. hamatum
which differs in much shorter phialides [(4.0–)5.0–7.7(–10.8) μm long], smaller conidia [(3.5–)4.0–4.7(–5.3) × (2.5–)2.7– 3.0(–3.3) μm], pachybasium-like other than verticillium-like conidiophores, and compact pustules with long hairs protruding from the surface (
Jaklitsch & Voglmayr 2015
).