Three interesting fungal species associated with the Asian House Gecko in Kunming, China Author Yang, Erfu 0000-0003-2385-6402 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People’s Republic of China & Master of Science Program in Applied Microbiology (International Program), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand & Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand & Environmental Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand & Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand & erfu 20170431 @ gmail. com; https: // orcid. org / 0000 - 0003 - 2385 - 6402 erfu20170431@gmail.com Author Tibpromma, Saowaluck 0000-0002-4706-6547 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People’s Republic of China & saowaluckfai @ gmail. com; https: // orcid. org / 0000 - 0002 - 4706 - 6547 saowaluckfai@gmail.com Author Dai, Dongqin 0000-0001-8935-8807 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People’s Republic of China & cicidaidongqin @ gmail. com; https: // orcid. org / 0000 - 0001 - 8935 - 8807 cicidaidongqin@gmail.com Author Promputtha, Itthayakorn 0000-0003-3376-4376 Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand & Environmental Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand & Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand & itthayakorn. p @ cmu. ac. th; https: // orcid. org / 0000 - 0003 - 3376 - 4376 itthayakorn.p@cmu.ac.th Author Mortimer, Peter E. 0000-0003-3188-9327 Centre for Mountain Futures (CMF), Kunming Institute of Botany, Kunming 650201, Yunnan, People’s Republic of China & peter @ mail. kib. ac. cn; https: // orcid. org / 0000 - 0003 - 3188 - 9327 peter@mail.kib.ac.cn Author Karunarathna, Samantha C. 0000-0001-7080-0781 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People’s Republic of China & samantha @ mail. kib. ac. cn; https: // orcid. org / 0000 - 0001 - 7080 - 0781 samantha@mail.kib.ac.cn text Phytotaxa 2022 2022-05-05 545 1 37 56 http://dx.doi.org/10.11646/phytotaxa.545.1.3 journal article 55671 10.11646/phytotaxa.545.1.3 1dffe0e1-c1e2-410b-ac72-492b5b1ed50f 1179-3163 6520349 Beauveria bassiana (Bals.-Criv.) Vuill., Bulletin de la Société Botanique de France 59: 40 (1912) ( Figure 5 ) Index Fungorum number: IF 199430 Saprobic or pathogenic on a dead Asian House Gecko, mycelium clusters on the forehead, recognized as a white, raised, fluffy region. Sexual morph: undetermined. Asexual morph: Aerial mycelium dense, raised, entire edge, white to yellowish, velutinous, powdery while sporulating, yellowish-brown from reverse. Vegetative hyphae 1–3 μm (x̅ = 1.7 μm, n = 30) wide, branched, hyaline, smooth, thick-walled, occasionally jagged wall, septate, constricted at the septum, terminal round, Conidiogenous cells 4–11 μm (x̅ = 7.5 μm, n = 20) long, 1–3 μm (x̅ = 2 μm, n = 20) wide, solitary or occurring in lateral clusters, cylindrical, subglobose to ampulliform. Conidia 2–3 × 1–2.5 μm (x̅ = 2.5 × 2 μm, n = 20), catenated, globose to ellipsoidal, hyaline, aseptate, smooth-walled. Culture characteristics: Colonies growing on PDA reach 15 mm in diameter after one week at 27 °C, and randomly sporulating within one month in PDA, above low convex, entire edge, fluffy, white. Reverse: yellowish brown. Sporulated within one month, visible the effuse, white clusters of mycelia with conidia, without pigments produced in PDA. Substratum: insect, Coleoptera ( Tzean et al. 1997 ) ; Fragaria ananassa ( Rigotti et al. 2003 ) ; Cosmopolites sordidus , Hypothenemus hampei , Leucoptera coffeella , Phyllophaga sp. , Plutella xylostella ( Delgado 2011 ) ; Vitis vinifera ( Elena et al. 2018 ) ; Cannabis sativa ( Punja et al. 2019 ) ; Coffea arabica ( Serrato-Diaz et al. 2020 ) . Distribution: Taiwan , China ( Tzean et al. 1997 ); Switzerland ( Rigotti et al. 2003 ); Nicaragua ( Delgado 2011 ); Spain ( Elena et al. 2018 ); Canada ( Punja et al. 2019 ); Puerto Rico ( Serrato-Diaz et al. 2020 ), China mainland (this study). Material examined: China , Yunnan Province , on dead Asian House Gecko, Peter E . Mortimer, F-5 (Herb. KUN-HKAS 122655), living culture, KUMCC 21-0468. Genbank numbers: ITS : OM 670163 , rpb1 : OM 681324 , rpb2 : OM 681325 , tef1-α : OM 681322 , Bloc: OM 681323 . Notes: Our isolate KUMCC 21-0468 fits with the characteristics of Beauveria bassiana by having an asexual morph producing globose to subglobose, catenated conidia, aseptate, hyaline, with globose to flask-shaped phialides; conidiogenous cells normally cluster on the terminals of branched hypha ( Paul Vuillemin et al. 1912 , Chen et al. 2013 ). The clade B. bassiana was analysed in detail by Khonsanit et al. (2020) . The ITS BLASTn results of our isolate highly overlapped with Beauveria bassiana strains BCC 34332, ARSEF 751 and BBC12907, while rpb1 , rpb2 and tef1-α show high matches to B. bassiana ARSEF 1040 (>99%). In addition, Bloc gene region showed 100% (1486/1492, 1 gap) similarity to B. bassiana ( ARSEF 4934), and our tree topology of the ML and BI analyses also shows KUMCC 21-0468 is closely related to B. bassiana ARSEF 1040 with high statistical supports ( Figure 4 ). Therefore, our isolate KUMCC 21-0468 is identified as Beauveria bassiana , which is a new host record based on morphological features and phylogenetic evidences.