A new species of Proprioseiopsis (Mesostigmata, Phytoseiidae), with a dichotomous key to reported species from Egypt Author Abo-Shnaf, Reham Vegetable and Aromatic Plant Mites Department, Plant Protection Research Institute, Agricultural Author Zaki, Ayman Y. Vegetable and Aromatic Plant Mites Department, Plant Protection Research Institute, Agricultural text Acarologia 2022 2022-03-25 62 2 352 358 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4511 journal article 10.24349/inzn-l21b 2107-7207 7160352 Proprioseiopsis salviae n. sp. Zoobank: CF38DCFC-FC88-43B6-A7BE-36FBD6BB843F ( Figures 1–2 ) Diagnosis — Seta Z4 longer than Z5 ; seta S2> S4> S5 , seta S4 about 1.4 times as long as S5 ; calyx of spermatheca saccular; genital shield broad, with posterior margin truncate; ventrianal shield longer than wide; genu I without macroseta, genu II with seven setae; fixed cheliceral digit with seven teeth, movable digit with one tooth. Female (Five specimens measured, Figs. 1 a–e ) Dorsum ( Fig. 1a ) – Dorsal shield with few lateral striae anteriad z4 , smooth elsewhere, where muscle marks are more evident; 370 (352–397) long and 287 (265–311) wide; with 16 pairs of setae and two pairs of sub-lateral setae ( r3 and R1 ), fifteen pairs of poroids and six pairs of solenostomes ( gd1 , gd2 , gd4 , gd5 , gd6 , gd9 ). Length of dorsal setae: j1 36 (34–39), j3 66 (62–69), j4 11 (9–13), j5 9 (8–10), j6 17 (16–20), J5 10 (9–10), z2 50 (47–55), z4 30 (26–33), z5 9 (8–10), Z1 20 (18–22), Z4 88 (86–92), Z5 69 (68–70), s4 85 (83–87), S2 25 (23–29), S4 23 (22–25), S5 16 (14–19), r3 28 (23–33), R1 21 (18–23). All setae smooth, except Z4 and Z5 , slightly serrated. Peritreme extending anteriorly to level of j1 . Venter ( Fig. 1b ) – Presternal area slightly striate. Sternal shield reticulate anterolateraly; smooth elsewhere, posterior margin slightly concave; with three pairs of setae ST ( 1-ST3 ) and two pairs of poroids ( iv1 and iv2 ). Setae ST4 and poroid iv ( 3 ) situated on rounded metasternal platelets. Distances ST1-ST1 50 (47–51), ST2-ST2 76 (73–82), ST3-ST3 87 (85–90), ST4-ST4 84 (81–87), ST1-ST3 60 (58–64). Genital shield smooth with three pairs of muscle marks; distance ST5-ST5 97 (92–100). Ventrianal shield subpentagonal, reticulate; 119 (114–125) long, 116 (111–124) wide at ZV2 level and 111 (107–118) wide at anus level; with three pairs of pre-anal setae ( JV1 , JV2 , and ZV2 ) and one pair of pre-anal round pores gv ( 3 ) posteromesad JV2 . Seta JV5 71 (68–74). Ventral setae smooth. Two pairs of punctate metapodal plates present. With four transversely elongate punctate platelets between genital and ventrianal shields. Unsclerotized cuticle laterad and posteriad ventrianal shield with four pairs of distinguishable poroids. Figure 1 Proprioseiopsis salviae n. sp. holotype female. a – Dorsal shield, b – Ventral shields, c – Spermathecae, d – Chelicera, e – Genu, tibia and basitarsus of leg IV. Figure 2 Proprioseiopsis salviae n. sp. paratype male. a – Chelicera showing spermatodactyl, b – Ventrianal shield. Spermatheca ( Fig. 1c ) – Calyx of spermatheca saccular, flaring slightly toward vesicle, 23 (21–25) long; atrium forked, with long major duct. Gnathosoma – Corniculi parallel to each other; about 18 (18–19) long, basal width of corniculus 4 (3–5), distance between tips of corniculi 10 (8–10). Fixed cheliceral digit 32 (31–33) long, with seven teeth; movable digit 30 (28–31) long, with one tooth; dorsal and antiaxial lyrifissures distinct ( Fig. 1d ). Legs ( Fig. 1e ) – Macrosetae present only on leg IV, sharp-tipped: Sge IV 48, Sti IV 34 (33–35), St IV 80 (73–86); chaetotaxy of genu II 2, 2/0, 2/0, 1; genu III 1, 2/1, 2/0, 1. Male (One specimen measured, Figs. 2 a–b ) Dorsum – Dorsal shield pattern as in adult female, but differs in the number of setae; with 18 pairs of setae ( r3 and R1 on dorsal shield); 287 long and 224 wide. Length of dorsal setae: j1 26, j3 (broken), j4 10, j5 9, j6 17, J5 9, z2 51, z4 38, z5 12, Z1 20, Z4 68, Z5 55, s4 59 , S2 22, S4 18 , S5 16 , r3 13, R1 16. All setae smooth, except Z4 and Z5 , slightly serrated. Peritreme extending anteriorly to level between j1 and j3 . Venter – Distances ST1-ST1 48, ST2-ST2 60, ST3-ST3 66, ST4-ST4 55, ST5-ST5 47, ST1- ST3 57, ST1-ST5 107. Ventrianal shield subtriangular, reticulate ( Fig. 2a ); 129 long and 155 wide at anterior corners; with three pairs of pre-anal setae, two pairs of poroids ivo () and a pair of pre-anal pores ( gv3 ). Seta JV5 35. Table 1 Differences between Proprioseiopsis salviae n. sp. and closer species of the “ belizensis species subgroup”.

Species	j3	j6	z2	z4	s4	Z4	Z5	S2	S4	S5	JV5	Sge IV	Sti IV	St IV	Number of teeth on fixed digit	References
P.basis	Not stated	10	40	25	93	106	85	20	Not stated	Not stated	65	35	27	78	3	Karg (1994)
P.belizensis	90–105	5–7	50–52	30–31	102–110	104–112	76–90	10–12	7–13	8–10	48–58	40–41	29–31	55–60	Not stated	Yoshida-Shaul & Chant (1991)
P.mauiensis	42	13	25	19	77	96	92	22	27	24	Not stated	53	45	82	4	Prasad (1968)
P.ovatus (Holotype)	64	12	42	22	88	101	90	17	16	12	Not stated	55	43	96	Multi-dentate	Moraes & McMurtry (1983)
P.ovatus	64 (57–69)	9 (8–10)	39 (32–44)	25 (19–34)	99 (91–106)	108 (88–120)	88 (67–107)	20 (14–26)	14 (9–16)	10 (9–11)	Not stated	58 (46–66)	41 (34–47)	84 (77–91)	5	Moraes et al . (2007)
80–89	13	38–42	27–35	103–108	100–107	73–88	11	9	9	Not stated	61	36	77	Not stated	Ferragut et al . (2010)
57–75	8–13	30–53	18–36	91–110	88–122	67–110	14–26	9–18	9–15	75–95	45–66	34–47	73–105	5–7	Kreitera et al . (2020)
P.putrephilus	38 (38–41)	8–10	30 (27–32)	21 (20–23)	65 (62–68)	71 (71–77)	72 (68–80)	9 (8–12)	9 (7–10)	13 (10–15)	50 (48–52)	51 (49–60)	30 (30–35)	44 (42–46)	4–5	Meshkov (1999)
P.rosellus	59	Not stated	z2 = z4	z4 = z2	99	113	90	Not stated	Not stated	Not stated	81	Not stated	Not stated	Not stated	Not stated	Chant (1959)
P. salviae n. sp.	66 (62–69)	17 (16–20)	50 (47–55)	30 (26–33)	85 (83–87)	88 (86–92)	69 (68–70)	25 (23–29)	23 (22–25)	16 (14–19)	71 (68–74)	48	34 (33–35)	80 (73–86)	7	Present Study

Gnathosoma – Corniculi parallel to each other; about 18 long, basal width of corniculus 3, distance between tips of corniculi 14. Fixed cheliceral digit 22 long, with six teeth; movable digit 21 long, with one tooth; dorsal and antiaxial lyrifissures distinct. Shaft of spermatodactyl 18 long with foot about 26 long, T-shaped toe and heel well developed ( Fig. 2b ). Legs – Macrosetae sharp-tipped: Sge IV 34, Sti IV 23, St IV 39; chaetotaxy of genua II and III as in adult female. Type specimens — Holotype female was collected from soil underneath Salvia splendens Sellow & Schultes ( Lamiaceae ), at Orman Botanical Garden (31°12′47.7468″E, 30°1′45.8148″N), Giza governorate , June 20, 2017 , one paratype male was collected from soil underneath the same previous substrate and locality, May 23, 2017 , one paratype female was collected from soil underneath Crinum asiaticum L. ( Amaryllidaceae ), at the same previous locality, April 04, 2017 , one paratype female was collected from soil underneath Dimorphotheca ecklonis (De Candolle) ( Asteraceae ), at the same previous locality, June 05, 2017, one paratype female was collected from soil underneath Xerochrysum bracteatum (Ventenat) Tzelev ( Asteraceae ), at the same previous locality, May 15, 2017 ; all deposited at the mite reference collection of the Egyptian Society of Acarology Museum ( ESAM ), Zoology and Agricultural Nematology Department, the Faculty of Agriculture, Cairo University, Giza governorate , Egypt . One paratype female was collected from soil underneath Pelargonium x hortorum Bailey ( Geraniaceae ), at the same previous locality, May 23, 2017 ; deposited at the mite collections of L’institut Agro (Montpellier SupAgro) conserved in UMR CBGP INRAE / IRD/ CIRAD / SupAgro/ Université de Montpellier, Montpellier, France . Etymology — The epithet salviae refers to “ Salvia ” the genus of the host plant Salvia ( splendens ), from which the holotype female and paratype male were collected. Remarks — This species belongs to belizensis species group as genu I has no macrosetae and to belizensis subgroup according to the form of calyx of spermatheca (Chand and McMurtry 2005). The new species closely resembles Proprioseiopsis basis Karg, 1994 ; P. belizensis ( Yoshida-Shaul & Chant, 1991 ) ; P. mauiensis ( Prasad, 1968 ) ; P. ovatus ( Garman, 1958 ) ; P. putrephilus Meshkov, 1999 and P. rosellus ( Chant, 1959 ) . Important differences between these species and the new species are listed in Table (1).