Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species
Author
Li, A. - H.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Author
Yuan, F. - X.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & North Minzu University, Yinchuan, Ningxia, 750030, China
Author
Groenewald, M.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands
Author
Bensch, K.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands
Author
Yurkov, A. M.
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, 38124, Germany
Author
Li, K.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Author
Han, P. - J.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Author
Guo, L. - D.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Author
Aime, M. C.
Purdue University, Department of Botany and Plant Pathology, West Lafayette, IN, 47901, USA
Author
Sampaio, J. P.
UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal & PYCC - Portuguese Yeast Culture Collection, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal
Author
Jindamorakot, S.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand
Author
Turchetti, B.
Department of Agriculture, Food and Environmental Sciences & Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, 74 - I- 06121, Italy
Author
Inacio, J.
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, BN 2 4 GJ, UK
Author
Fungsin, B.
TISTR Culture Collection, Thailand Institute of Scientific and Technological Research (TISTR), 35 M 3, Technopolis, Khlong Ha, Khlong Luang, Pathum Thani, 12120, Thailand
Author
Wang, Q. - M.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & College of Life Sciences, Hebei University, Baoding, Hebei Province, 071002, China
wangqm@im.ac.cn
Author
Bai, F. - Y.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
baify@im.ac.cn
text
Studies In Mycology
2020
2020-01-28
96
17
140
journal article
10.1016/j.simyco.2020.01.002
PMC7082220
32206137
10497182
Kondoa daliangziensis
Q.M. Wang, F.Y. Bai & A.H. Li
sp. nov.
MycoBank MB832014.
Fig. 12M, N
.
Etymology
: the specific epithet
daliangziensis
refers to the geographic origin of the type strain, Daliangzi River National Forest Park, Heilongjiang.
Culture characteristics
: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 2.7–4.4 × 4.3– 8.4 μm and single, budding is polar (
Fig. 12M
), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale orange, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.6– 3.1 × 7.1– 8.6 μm (
Fig. 12N
).
Physiological and biochemical characteristics
: Glucose fermentation is absent. Glucose, sucrose (variable), maltose, cellobiose, trehalose, melezitose, L-arabinose (variable), ethanol (variable), glycerol, ribitol (variable), D-mannitol (variable), D-glucitol (variable), salicin (variable) and DL-lactate (variable) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, raffinose, inulin, soluble starch, D-xylose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, galactitol, Methyl-α- D-glucoside, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride (weak) and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 22–23 °C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.
Physiologically,
Kon. daliangziensis
and
Kon. ribitophobia
are difficult to distinguish from each other. The latter can grow at 25 °C, but the former does not.
Kon. daliangziensis
differs from
Kon. gutianensis
in its inability to assimilate galactose and inulin and its ability to assimilate L-lysine (
Table S1.17
).
Typus
:
China
,
Daliangzi river national forest park
,
Heilongjiang
province, obtained from a leaf of an unidentified plant,
Aug. 2014
,
Q.-M. Wang
(
holotype
CGMCC 2.5610
T
preserved in a metabolically inactive state, ex-type CBS 13974 = HLJ22A8).