New species of Asphondylia (Diptera, Cecidomyiidae, Asphondyliini) from Brazil Author Maia, Valéria Cid Author Oliveira, Lázaro Araújo text Papéis Avulsos de Zoologia 2021 Pap. Avulsos Zool., S. Paulo 2021-06-08 61 1 20 http://dx.doi.org/10.11606/1807-0205/2021.61.57 journal article 10.11606/1807-0205/2021.61.57 1807-0205 5213244 C6D19E17-7204-47E9-A63C-15E98BEB306B Asphondylia ingaiensis Maia , sp. nov. ( Figs. 2-8 ) Diagnosis: Male hypoproct truncated apically, deeply bilobed; ovipositor with needle part about 2.0-2.5 X length 7 th sternite; pupa: antennal horn with apical part about 0.6 X length basal part, upper frontal horn simple, 0.5 X length antennal horn, lower frontal horn tridentate 0.3 X length antennal horn, 8 th abdominal segment with 9-12 dorsal spines in the posterior row. Figure 1. Length of cephalic horns indicated by black bars. (A) Antennal horns,(B) Upper facial horn,(C) Lower facial horn. Figure 2. Asphondylia ingaiensis sp.nov. , male.(A) Head,ventral view,(B) 1 st and 2 nd flagellomeres,(C) 3 rd flagellomere. Figure 3. Asphondylia ingaiensis sp.nov. , male.(A) Foreleg,tarsal claw and empodium,lateral view (B) Midleg,first tarsomere,lateral view (C)Wing. Figure 4. Asphondylia ingaiensis sp.nov. , male.(A) Abdomen,lateral view,(B) 6 th- 8 th abdominal segments,lateral view. Male: Body length: 3.40-3.90 mm (N = 2). Head ( Fig. 2A ): 0.50-0.55 mm long, 0.50-0.55 mm wide (N = 2), eye facets circular, closely appressed; antennae: flagellomeres 1 and 2 not fused ( Fig. 2B ), scape truncated conical, setose, 0.15- 0.14 mm long, 0.05 mm wide (N = 2), pedicel globose, setose, 0.06 mm long, 0.05 mm wide, 1 st- 9 th flagellomeres cylindrical, all 0.05 mm wide, circumfila longitudinally wavy, dense, anastomosing, equally spread along segments ( Fig. 2C ), 1 st flagellomere 0.22-0.24 mm long, 2 nd flagellomere 0.22- 0.21 mm long, 3 rd flagellomere 0.22- 0.20 mm long,4 th- 8 th flagellomeres 0.20 mm long, 9 th flagellomere 0.18-0.19 mm long, 10 th- 12 th flagellomeres missing (N = 2), proportion flagellomere neck-node 1:9; frons with 30-32 setae (male, N = 2); mouth parts: labrum long-attenuated; hypopharynx of the same shape of labrum, with long lateral setulae, anteriorly directed; labella elongate and convex, 0.13 mm long, 0.04 mm wide (N = 1), with lateral and mesal setae; palpus 0.33-0.39 mm long:1 st segment globoid, 0.06-0.07 mm long, 0.04-0.05 m wide, 2 nd segment cylindrical, 0.08-0.11 mm long, 0.04-0.05 mm wide, 3 rd segment fusiform, 0.19-0.21 mm long, 0.05 mm wide (N = 2), all segments with setae. Thorax: scutum with two dorsocentral rows of setae, setae more abundant anteriorly, two groups of lateral setae more abundant proximally, extending from base to distal margin, scales intermixed; scutellum with scattered setae; anepimeron setose; remaining pleural sclerites bare; legs: tarsal claws curved beyond midlength, anisomorphic, less robust on foreleg than on mid and hindlegs, empodium as long as claws ( Fig. 3A ); first tarsomere of each leg with an apical hook-like projection 0.03 mm long ( Fig. 3B ); wing: length 2.70-2.90 mm (N = 3) ( Fig. 3C ). Abdomen ( Fig. 4A ): trichoid sensilla not visible; 1 st- 7 th tergites sclerotized, rectangular with a posterior row of setae, few scattered lateral setae and mostly covered elsewhere with scales,8 th tergite bare, narrow, with mesal reentrance in the anterior margin ( Fig. 4B ); 2 nd- 8 th sternites sclerotized, rectangular,narrower than tergites,with a posterior row of setae, several setae and midlength, few lateral setae, and mostly covered elsewhere with scales; 8 th sternite with setae at ⅔ distal and mostly covered elsewhere with scales. Terminalia ( Fig. 4B ): gonocoxite short and stout, 0.16 mm long, 0.12 mm wide; gonostylus ovoid, 0.07 mm long, 0.04 mm wide, teeth 0.015 mm long, 0.020 mm wide (N = 3); hypoproct truncated apically and deeply bilobed. Figure 5. Asphondylia ingaiensis sp. nov. , female. (A) Head, ventral view, (B) 3 rd flagellomere, (C) midleg, tarsal claw and empodium, lateral view, (D) Abdomen (4 th segment to end),lateral view. Female: Body length: 3.80-4.60 mm (N = 4). Head ( Fig. 5A ): about the same as male, antennae: scape 0.15 mm long (N = 1), pedicel 0.06 mm long (N = 1), 1 st- 8 th flagellomeres cylindrical, all 0.05 mm wide, circumfila comprising two longitudinal bands connected sub basally and apically by two transverse bands ( Fig. 5B ), 1 st flagellomere 0.28 mm long (N = 1), 2 nd flagellomere 0.26 mm long (N = 1), 3 rd flagellomere 0.25 mm long (N = 1), 4 th flagellomere 0.23 mm long (N = 1), 5 th flagellomere 0.24 mm long (N = 1), 6 th flagellomere 0.22 mm long (N = 1), 7 th- 8 th flagellomeres 0.21 mm long (N = 1), 9 th- 12 th flagellomeres missing; mouthparts: labellum 0.07 mm long, 0.04 mm wide at midlength (N = 2), palpus 0.22-0.24 mm long (N = 2): 1 st segment globose 0.03 mm long and 0.03 mm wide (N = 2), 2 nd segment cylindrical 0.07 mm long, 0.02 mm wide at midlength (N = 2), 3 rd segment claviform 0.12-0.14 mm long and 0.03 mm wide at midlength (N = 2). Thorax: wing length: 3.00- 4.80 mm (N = 7); tarsal claws more sclerotized and robust than in male ( Fig. 5C ). Abdomen ( Fig. 5D ): trichoid sensillae not visible, 1 st- 7 th tergites as in male, 8 th tergite with posterior margin with lobes 0.28-0.30 mm long (N = 3), 2 nd- 6 th sternites as in male, 7 th sternite with anterior margin and posterior margins more sclerotized mesally, 0.65-0.70 mm long, 1.54-1.75 X length sternite 6 (N = 4), setose (except basally), mostly covered elsewhere with scales; sternite 8 not sclerotized;ovipositor ( Fig. 6A ): needle part 1.35-1.70 mm long, 2.08-2.42 X length sternite 7 (n = 4). Other characters as in male. Figure 6. Asphondylia ingaiensis sp.nov. (A) Female 6 th abdominal segment to end,lateral view,(B-C) Pupa,(B) Head,frontal view,(C) Lower facial horn. Pupa: Color: brownish. Body length: 4.20 mm (N = 1). Head ( Fig. 6B ): antennal horn 0.28 mm long (N = 1), triangular, with inner margin serrated; apical seta 0.04 mm long (N = 1); upper facial horn simple, 0.14 mm long (N = 2), triangular; lower facial horn tridentate, 0.09 mm long (N = 2), triangular ( Fig. 6C ); two pairs of lower facial papillae: one pair setose, the other bare; three pairs of lateral facial papillae: one pair setose, two bare; upper cephalic margin thickened laterally. Thorax: prothoracic spiracle 0.13-0.14 mm long, as long as antennal basal width, setiform, slightly curved (N = 2), integument wrinkly ( Figs. 7A, B ). Abdomen: segments 2-8 with transverse rows of crescent dorsal spines at basal half ( Fig. 8A ); posterior row with 23-25 spines in the 2 nd segment, 22-25 in the 3 rd , 22-24 in the 4 th , 21-24 in the 5 th , 18-24 in the 6 th , 13-18 in the 7 th and 9-12 in the 8 th . Larva: Body length: 3.20 mm (N = 1); cephalic head 0.05 mm long, 0.11 mm wide (N = 1). Spatula quadridentate, 0.34 mm long (n = 1), lateral teeth 0.05 mm long, mesal teeth 0.04 mm long (N = 1) ( Fig. 8B ); four setose lateral papillae 0.01 mm long of each side of spatula.