From ed20a3541122911d9f0e6acb2f5862462a47202b Mon Sep 17 00:00:00 2001 From: ggserver Date: Mon, 26 Aug 2024 12:07:22 +0000 Subject: [PATCH] Add updates up until 2024-08-26 12:01:16 --- .../28/634C2847FF9BFFBCFF1CBD94FC6AFD96.xml | 354 ++++++++++++++++++ 1 file changed, 354 insertions(+) create mode 100644 data/63/4C/28/634C2847FF9BFFBCFF1CBD94FC6AFD96.xml diff --git a/data/63/4C/28/634C2847FF9BFFBCFF1CBD94FC6AFD96.xml b/data/63/4C/28/634C2847FF9BFFBCFF1CBD94FC6AFD96.xml new file mode 100644 index 00000000000..8d186401767 --- /dev/null +++ b/data/63/4C/28/634C2847FF9BFFBCFF1CBD94FC6AFD96.xml @@ -0,0 +1,354 @@ + + + +Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China + + + +Author + +Zhou, Si-Xuan +0000-0002-2589-136X +Institute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550005, China; +kerrybaiye@163.com + + + +Author + +Bao, Dan-Feng +0000-0002-5697-4280 +Engineering and Research Center for Southwest Biopharmaceutical Resource of National Education Ministry of China, Guizhou University, Guiyang, 550025, Guizhou Province, People’s Republic of China; + + + +Author + +Zhang, Tao +0009-0004-0853-6181 +Institute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550005, China; + + + +Author + +Xiao, Yuan-Pin +0000-0003-1730-3545 +School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang, Guizhou 550003, P. R. China; + + + +Author + +Tang, Yuan-Jiang +0000-0002-7281-0219 +Institute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550005, China; + + + +Author + +Jing, Zhang +0000-0002-7281-0219 +Institute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550005, China; + + + +Author + +Lu, Yu-Xi +0009-0005-5805-9181 +Institute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550005, China; + + + +Author + +Shi, Kai-Zhi +0000-0001-7255-7180 + +text + + +Phytotaxa + + +2024 + +2024-03-07 + + +640 + + +1 + + +1 +15 + + + + +http://dx.doi.org/10.11646/phytotaxa.640.1.1 + +journal article +10.11646/phytotaxa.640.1.1 +1179-3163 +13213342 + + + + + + +Diaporthe berchemiae +S.X. Zhou + + +sp. nov. + + + + +Index Fungorum number: IF 900644; Facesoffungi number: FoF 15295; +FIGURE. 4 + + + + +Etymology: Referring to the host plant + +Berchemia polyphylla +var. +leioclada + +, on which the fungus was collected + + + +Holotype +: +KUN-HKAS 131985 + + + +Endophytic +in fresh leaves of + +Berchemia polyphylla +var. +leioclada + +. +Sexual morph: +not observed. +Asexual morph: +Conidiomata +pycnidial in culture on PDA, globose or irregular, brown to dark brown, solitary or in groups, embedded, erumpent, white to cream conidial drops exuded from ostioles. +Conidiomatal wall +brown to dark brown, composed of several layers of brown to dark brown cells of +textura angularis +. +Conidiophores +9–17 × 2–3 µm ( +x += 12.9 × 2.3μm, n = 25), cylindrical to subcylindrical, tapering to straight towards the apex, 1–2 septate, densely aggregated, hyaline, subhyaline to pale brown at the base, smooth-walled. +Conidiogenous cells +phialidic, terminal, cylindrical to subcylindrical, hyaline. + +Alpha +conidia + +5–6 × 1.5–2.5 µm ( +x += 5.6 × 2μm, n = 32), ellipsoidal to obovoid, or fusoidellipsoid, rounded or subobtuse at apex, acutate or subtruncate at the base, hyaline, aseptate, smooth-walled, with 0-1 small-guttules. + +Beta conidia + +19–26 × 1–1.5 μm ( +x += 22.4 × 1.3μm, n = 30), filiform, slightly curved, tapering towards the ends, aseptate, hyaline, not guttulate. + +Gamma +conidia + +not observed. + + +Cultural characteristics: Cultures on MEA initially white, growing slowly and entirely covering the +9cm +Petri dish after 14 days, velvety appearance with whit aerial mycelium, white on surface, white to pale brown on reverse. + + + +FIGURE 4. + +Diaporthe berchemiae + +(ex-type culture, GMBCC 20000). +a, b +Front and reverse colony on MEA. +c, d +Conidiomata on PDA. +e–g +Conidiophores. +h–k +Conidiogenous cells and conidia. +l–o +Alpha conidia. +p-s +Beta conidia +. Scale bars: e–g, i–k = 10 μm; h, l–s = 5 μm. + + + +Material examined: +CHINA +, +Guizhou Province +, from + +Berchemia polyphylla +var. +leioclada + +leaves, +27 June 2022 +, S.X. Zhou, A12J-1 (KUN-HKAS 131985, +holotype +), ex-type culture GMBCC 20000. + +GenBank numbers: ITS = PP049022, HIS3 = PP056095, TEF1-α = PP056096, TUB2 = PP056097. + +Notes: In our phylogenetic analysis, + +Diaporthe berchemiae + +formed a distinct lineage between + +D. ellipsospora + +and the clade of + +D. nanjingensis + +and + +D. jinxiu + +( +FIGURES. 5 +, S +2 +). + +Diaporthe ellipsospora + +was introduced based on the sexual morph ( + +Dissanayake +et al. +2020 + +), while + +D. berchemiae + +is reported by the asexual morph. Therefore, we are unable to compare the morphology between these two species, but phylogenetically they are distinct ( +Figures 5 +, S +2 +). The comparison of ITS region between + +D. berchemiae + +and + +D. ellipsospora + +revealed 41 bp (7.9%) differences which strongly support our new isolate as a new species. + + + + + +Diaporthe berchemiae + +is similar to + +D. jinxiu + +and + +D. nanjingensis + +in having hyaline, cylindrical, smooth-walled conidiophores, phialidic, cylindrical conidiogenous cells, and hyaline, aseptate, ellipsoidal alpha conidia. However, + +D. berchemiae + +differs from + +D. jinxiu + +and + +D. nanjingensis + +in having 0-1 small-guttulate alpha conidia, whereases, the conidia of + +D. jinxiu + +and + +D. nanjingensis + +with two large guttules. In addition, the alpha conidia of + +D. jinxiu + +are rounded at both ends, while the alpha conidia are rounded or subobtuse at the apex and acutate or subtruncate at the base in + +D. berchemiae +( + +Wang +et al. +2021 + +) + +. + +Diaporthe berchemiae + +has much shorter beta conidia (19–26 vs. 29.7–37.1(-39.7) um) than those of + +D. nanjingensis + +and the conidiogenous cells of + +D. nanjingensis + +are muti-guttulate, which were not observed in + +D. berchemiae +( + +Wan +et al. +2023 + +) + +. The PHI test revealed no significant recombination event between + +Diaporthe berchemiae + +and the closely related taxa (Φw = 0.22) ( +FIGURE. 6 +). Therefore, we introduce our new isolate as a new species based on both phylogeny and morphological analyses. + + + + \ No newline at end of file