From d714031acd96a95ee5df1231fc59fce54e5b8b51 Mon Sep 17 00:00:00 2001 From: ggserver Date: Tue, 7 Jan 2025 19:36:19 +0000 Subject: [PATCH] Add updates up until 2025-01-07 19:31:16 --- .../D2/F10FD265F3535914B4D8FA1672AF05B2.xml | 418 ++++++++++++++++ .../9A/FF6C9A8BE57D5D7E9E2A5620AE7E46C8.xml | 469 ++++++++++++++++++ 2 files changed, 887 insertions(+) create mode 100644 data/F1/0F/D2/F10FD265F3535914B4D8FA1672AF05B2.xml create mode 100644 data/FF/6C/9A/FF6C9A8BE57D5D7E9E2A5620AE7E46C8.xml diff --git a/data/F1/0F/D2/F10FD265F3535914B4D8FA1672AF05B2.xml b/data/F1/0F/D2/F10FD265F3535914B4D8FA1672AF05B2.xml new file mode 100644 index 00000000000..9bfd54a5f24 --- /dev/null +++ b/data/F1/0F/D2/F10FD265F3535914B4D8FA1672AF05B2.xml @@ -0,0 +1,418 @@ + + + +Two new species of Xylaria (Xylariaceae, Ascomycota) associated with fallen leaves in Hainan Tropical Rainforest National Park, China + + + +Author + +Pan, Xiaoyan +0000-0002-3244-7486 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Zongzhu +https://orcid.org/0009-0003-1464-1159 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Yiqing +https://orcid.org/0009-0000-4416-6932 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Lei, Jinrui +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Xiaohua +https://orcid.org/0009-0003-5421-5509 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Wu, Tingtian +https://orcid.org/0009-0008-8934-9255 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Li, Yuanling +https://orcid.org/0009-0005-4826-6021 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + +text + + +MycoKeys + + +2025 + +2025-01-07 + + +112 + + +17 +34 + + + +journal article +10.3897/mycokeys.112.140487 + + + + + +Xylaria acifer +Xiao Y. Pan + +sp. nov. + + + + +Fig. 2 + + + + + +Holotype +. + + + + +China +• +Hainan Province +, +Diaoluoshan Area of Hainan Tropical Rainforest National Park +; + +18°43'35"N +, +109°52'11"E + +; + +elevation +973 m + +; +on fallen leaves +, + +18 June 2023 + +, +Xiaoyan Pan +( +HAFFR 122 +). GenBank accession numbers +PQ 483147 +(ITS), +PQ 498329 +( +TUB 2 +), and +PQ 498326 +( +RPB 2 +). + + + + + + + + +Xylaria acifer + +(HAFFR 122) a stromata on leaves +b +stromatal surface and ostioles (arrow) +c +section through stroma, showing perithecia +d +ascus in Melzer’s reagent +e, f +ascospore showing non-cellular appendages in 1 % SDS (arrows) +g, h +ascal apical ring in Melzer’s reagent +i +ascospores in Melzer’s reagent +j +ascus in water +k +ascospore showing a nearly spore-length straight germ slit in Melzer’s reagent +l +ascospore under SEM. Scale bars: 1 cm ( +a +); 200 µm ( +b, c +); 10 µm ( +d – k +); 5 µm ( +l +). + + + + + +Diagnosis. + + +Differs from + +X. hedyosmicola + +by its smaller ascospores. Differs from + +X. vittatipiliformis + +by the absence of band-like stripes on its stromata surfaces and its smaller ascospores. Differs from + +X. vittiformis + +by the absence of band-like stripes on its stromata surfaces. + + + + +Etymology. + + + +“ +acifer + +” refers to the needle-like shape of the stromata. + + + + +Teleomorph. + + +Stromata +upright, solitary, needle-like, unbranched, +25–50 mm +total length; acute sterile apex, +2–10 mm +; fertile portion +3–10 mm +long × +0.8–1.2 mm +diam., elongate cylindrical, composed of tightly arranged perithecia; stipe glabrous, +15–40 mm +long × +0.5–1 mm +diam., longitudinally striate, the base slightly swollen; surface roughened, black, with conspicuous to half-exposed perithecial mounds; interior white to creamy; texture soft. +Perithecia +spherical, 250–500 µm diam. +Ostioles +papillate. +Asci +with eight ascospores arranged in uniseriate manner, cylindrical, 90–150 µm total length, spore-bearing part 60–85 µm long × 5.5–7.5 µm wide, stipe 30–70 µm long, with a bluing apical ring in Melzer’s reagent, tubular to slightly urn-shaped, 1.8–3.5 µm high × 1.4–2.5 µm diam. +Ascospores +brown to dark brown, unicellular, ellipsoid to fusiform, inequilateral, with narrowly rounded ends, smooth, (9.3 –) 10–11 (– 12) × 3.7–4.7 µm (M = 10.6 × 4.2 µm, N = 40), with straight germ slit nearly the full length of the spore on the flattened side, a hyaline sheath visible in 1 % SDS, swollen at both ends, forming non-cellular appendages. + + + + +Remarks. + + + +Xylaria acifer + +clusters with + +X. hedyosmicola + +in the phylogenetic tree, and they share some similarities in stromatal morphology, yet they also exhibit distinct differences. The stromata of + +X. hedyosmicola + +possess more prominent perithecial mounds (half-exposed to fully exposed), and its ascospores are brown, ellipsoid, and larger (( 12 –) 13–15 (– 16.7) × (6 –) 6.5–7.5 (– 8.5) µm) ( +Pan et al. 2022 +). + +Xylaria vittatipiliformis + +Y. - M. Ju, H. - M. Hsieh & Fournier and + +X. vittiformis + +Y. - M. Ju & H. - M. Hsieh are morphologically similar to + +X. acifer + +. + +Xylaria vittatipiliformis + +can be distinguished by its band-like striped grayish brown outer peeling layer and larger ascospores (( 10 –) 11–12 (– 12.5) × (5.5 –) 6–7 (– 7.5) µm) ( +Ju and Hsieh 2023 +). + +Xylaria vittiformis + +is separated from + +X. acifer + +by its grayish brown outer peeling layer split into band-like stripes, smaller perithecia (150–200 μm broad), and ellipsoid ascospores, (8 –) 8.5–10 (– 11) × (4 –) 4.5–5 (– 5.5) µm ( +Ju and Hsieh 2023 +). + + + +Xylaria appendiculatoides +Y. M. Ju & H. M. Hsieh + +, + +X. filiformis + +, and + +X. simplicissima + +are also similar to + +X. acifer + +in stromatal morphology but are distinctly different. + +Xylaria appendiculatoides + +has stromata with sharper ostioles (conic-papillate, tilting upwards) and larger ascospores (( 14 –) 15–16 (– 17) × (6.5 –) 7.0–7.5 (– 8) µm) ( +Ju and Hsieh 2023 +). + +Xylaria filiformis + +and + +X. simplicissima + +have ascospores that are markedly different from those of + +X. acifer + +. + +Xylaria filiformis + +has light brown ascospores that are larger (( 9.5 –) 11.5–13.5 (– 14.5) × (4 –) 4.5–5.5 (– 6) µm) ( +Ju and Hsieh 2023 +). + +Xylaria simplicissima + +has larger ascospores (( 15 –) 16.5–19 (– 21.5) × (5 –) 5.5–6.5 (– 7.5) µm) that lack non-cellular appendages ( +Ju and Hsieh 2023 +). Additionally, + +X. acifer + +, + +X. filiformis + +, and + +X. simplicissima + +are distributed in different clades in the phylogenetic tree. + +Xylaria maitlandii +(Dennis) D. Hawksw. + +has a somewhat similar stromatal shape to + +X. acifer + +. However, + +X. maitlandii + +has hairy stromata, which clearly distinguishes it from + +X. acifer + +( +Ju and Hsieh 2023 +). + + + + +Additional specimen examined. + + + +China +• +Hainan Province +, +Diaoluoshan Area of Hainan Tropical Rainforest National Park +; + +18°43'31"N +, +109°52'14"E + +; + +elevation +937 m + +; +on fallen leaves +, + +18 June 2023 + +, +Xiaoyan Pan +( +HAFFR 130 +). GenBank accession numbers +PQ 483150 +(ITS) and +PQ 498328 +( +RPB 2 +) + +. + + + + \ No newline at end of file diff --git a/data/FF/6C/9A/FF6C9A8BE57D5D7E9E2A5620AE7E46C8.xml b/data/FF/6C/9A/FF6C9A8BE57D5D7E9E2A5620AE7E46C8.xml new file mode 100644 index 00000000000..fa774ab17d9 --- /dev/null +++ b/data/FF/6C/9A/FF6C9A8BE57D5D7E9E2A5620AE7E46C8.xml @@ -0,0 +1,469 @@ + + + +Two new species of Xylaria (Xylariaceae, Ascomycota) associated with fallen leaves in Hainan Tropical Rainforest National Park, China + + + +Author + +Pan, Xiaoyan +0000-0002-3244-7486 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Zongzhu +https://orcid.org/0009-0003-1464-1159 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Yiqing +https://orcid.org/0009-0000-4416-6932 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Lei, Jinrui +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Chen, Xiaohua +https://orcid.org/0009-0003-5421-5509 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Wu, Tingtian +https://orcid.org/0009-0008-8934-9255 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + + + +Author + +Li, Yuanling +https://orcid.org/0009-0005-4826-6021 +Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, 571100, China & Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, 571100, China + +text + + +MycoKeys + + +2025 + +2025-01-07 + + +112 + + +17 +34 + + + +journal article +10.3897/mycokeys.112.140487 + + + + + +Xylaria tuberculosa +Xiao Y. Pan + +sp. nov. + + + + +Fig. 3 + + + + + +Holotype +. + + + + +China +• +Hainan Province +, +Diaoluoshan Area of Hainan Tropical Rainforest National Park +; + +18°43'33"N +, +109°52'20"E + +; + +elevation +932 m + +; +on fallen leaves +, + +18 June 2023 + +, +Xiaoyan Pan +( +HAFFR 123 +). GenBank accession numbers +PQ 483148 +(ITS), +PQ 498330 +( +TUB 2 +), and +PQ 498327 +( +RPB 2 +). + + + + + + + + +Xylaria tuberculosa + +(HAFFR 123) a stromata on leaves +b, d +stromatal surface and ostioles (arrow) +c +section through stroma, showing perithecia +e, f +ascus in 1 % SDS +g +ascospores in water +h +ascal apical ring in Melzer’s reagent +i +ascospore showing a slightly shorter than spore-length straight germ slit in water +j +ascospore showing non-cellular appendages in 1 % SDS (arrows) +k, l +ascospores under SEM. Scale bars: 0.5 cm ( +a +); 200 µm ( +b – d +); 10 µm ( +e – j, l +); 5 µm ( +K +). + + + + + +Diagnosis. + + +Differs from + +X. acifer + +, + +X. hedyosmicola + +, + +X. petchii + +, and + +X. polysporicola + +by having smaller ascospores. + + + + +Etymology. + + + +“ +tuberculosa + +” refers to the appearance of the stromata surface, which resembles segmented tubercles formed by the clustered perithecia. + + + + +Teleomorph. + + +Stromata +upright, solitary to scattered, cylindrical, unbranched, +5–10 mm +total length; acute sterile apex, +0.1 mm +; fertile portion +3–6 mm +long × +0.3–1 mm +wide, cylindrical, composed of tightly packed perithecia; stipe glabrous, +1.8–5 mm +long × +0.2–0.5 mm +wide, longitudinally striate, the base slightly swollen; surface roughened, black, with half-exposed to fully exposed perithecial mounds; interior white to creamy; texture soft. +Perithecia +spherical, 180–350 µm diam. +Ostioles +papillate. +Asci +with eight ascospores arranged in uniseriate manner, cylindrical, 70–105 µm total length, spore-bearing part 50–70 µm long × 5–7 µm wide, stipe 25–45 µm long, with a bluing apical ring in Melzer’s reagent, tubular to slightly urn-shaped, 1.8–2.6 µm high × 1.2–2.5 µm wide. +Ascospores +brown, unicellular, ellipsoid, inequilateral, with narrowly rounded ends, smooth, 7–8 (– 9) × 3.3–4.3 µm (M = 7.6 × 3.8 µm, N = 40), with straight germ slit slightly shorter than the spore length on the flattened side, and a hyaline sheath visible in 1 % SDS, swollen at both ends, forming non-cellular appendages. + + + + +Remarks. + + + +Xylaria tuberculosa + +forms a distinct branch in the phylogenetic tree with + +X. acifer + +, + +X. betulicola + +, + +X. crinalis + +, + +X. filiformis + +, + +X. hedyosmicola + +, + +and +X. simplicissima + +, but it is morphologically different from these six species. + +Xylaria acifer + +has a needle-like stroma with flatter perithecial mounds and larger ascospores (9.3–12 × 3.7–4.7 µm), while + +X. betulicola + +, + +X. crinalis + +, + +X. filiformis + +, + +X. hedyosmicola + +, + +and +X. simplicissima + +all have stromata with long sterile apices and significantly larger ascospores, measuring (9.5–21.5 × 3.5–8.5 µm). The ascospores of + +X. betulicola + +, + +X. crinalis + +, + +X. filiformis + +, + +X. hedyosmicola + +, and + +X. simplicissima + +measure (11.5 –) 12–14 (– 15) × 5–6 µm, (14 –) 15–16.5 (– 17.5) × (3.5 –) 4–5 (– 6) µm, (9.5 –) 11.5–13.5 (– 14.5) × (4 –) 4.5–5.5 (– 6) µm, (12 –) 13–15 (– 16.7) × (6 –) 6.5–7.5 (– 8.5) µm, and (15 –) 16.5–19 (– 21.5) × (5 –) 5.5–6.5 (– 7.5) µm, respectively ( +Ma and Li 2018 +; +Pan et al. 2022 +; +Ju and Hsieh 2023 +). + + + +Xylaria petchii +Lloyd + +and + +X. polysporicola +Hai X. Ma & X. Y. Pan + +are similar to + +X. tuberculosa + +morphologically, as they share cylindrical stromata growing on fallen leaves. +Pan et al. (2024) +described + +X. petchii + +from +China +, noting that this species has a variable fertile portion of the stromata and ascospores measuring (8.5 –) 10–12.5 (– 15) × (4.5 –) 5–6.5 (– 7) µm, which clearly differentiate it from + +X. tuberculosa + +. + +Xylaria polysporicola + +differs from + +X. tuberculosa + +by having a larger apical ring (2.5–4.5 µm high × 2–3.2 µm broad) and larger ascospores (( 11.5 –) 12.5–14.5 (– 15) × 5.5–8 µm) ( +Pan et al. 2022 +). Furthermore, + +X. tuberculosa + +, + +X. petchii + +, and + +X. polysporicola + +are clearly separated in the phylogenetic tree. + + + +Xylaria himalayensis +Narula & Rawla + +and + +X. meliacearum +Læssøe + +also resemble + +X. tuberculosa + +in morphology. + +Xylaria himalayensis + +can be distinguished by its hairy stipe and larger ascospores (( 11.5 –) 13–15 (– 15.5) × (4.5 –) 5–5.5 (– 6) µm) that lack non-cellular appendages ( +Ju et al. 2018 +). + +Xylaria meliacearum + +can be differentiated by its stromata covered with a sulfur-yellow outer layer and larger ascospores (( 19 –) 21.5–27.5 (– 31.5) × (5 –) 5.5–7 (– 8) µm) ( +Ju and Hsieh 2023 +). + +Xylaria tentaculata +Ravenel ex Berk. + +is somewhat similar to + +X. tuberculosa + +in morphology, but + +X. tentaculata + +has significantly larger ascospores (20.6–24.4 µm × 8.9–10.8 µm) ( +Kim et al. 2016 +). + + + + +Additional specimen examined. + + + +China +• +Hainan Province +, +Diaoluoshan Area of Hainan Tropical Rainforest National Park +; +18°43'31"N +, +109°52'19"E +; + +elevation +929 m + +; +on fallen leaves +, + +26 February 2023 + +, +Xiaoyan Pan +( +HAFFR 63 +). GenBank accession numbers +PQ 483149 +(ITS) and +PQ 498331 +( +TUB 2 +) + +. + + + + \ No newline at end of file