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<document id="9AA63C2B9D7CEBF2C66E5C2FE46E5E76" ID-DOI="10.11646/phytotaxa.338.3.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13719995" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1725658270060" checkinUser="felipe" docAuthor="Błaszkowski, Janusz, Ryszka, Przemysław &amp; Kozłowska, Anna" docDate="2018" docId="0383475BFFFBFFA574E07C93CE7BF8E7" docLanguage="en" docName="phytotaxa.338.3.2.pdf" docOrigin="Phytotaxa 338 (3)" docSource="http://dx.doi.org/10.11646/phytotaxa.338.3.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Dominikia litorea Blaszk. &amp; Kozlowska 2018, sp. nov." docType="treatment" docVersion="3" lastPageNumber="246" masterDocId="FFBA3F23FFFEFFA074687D67CB1DFFF8" masterDocTitle="Dominikia litorea, a new species in the Glomeromycotina, and biogeographic distribution of Dominikia" masterLastPageNumber="254" masterPageNumber="241" pageNumber="246" updateTime="1725669970784" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="5D53E2A7EE9CC12ACEFF96B9C08F472E">Dominikia litorea, a new species in the Glomeromycotina, and biogeographic distribution of Dominikia</mods:title>
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<mods:namePart id="670637A1C321C31CBECD215FBCC4530F">Błaszkowski, Janusz</mods:namePart>
<mods:affiliation id="4F38DE7E99B857CDD2BCEA2B74EF3381">Department of Ecology, Protection and Shaping of Environment, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71 - 434 Szczecin, Poland</mods:affiliation>
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<mods:namePart id="DFD27C3A6AC79C44AE10E8242CAA9318">Ryszka, Przemysław</mods:namePart>
<mods:affiliation id="EDA5614B9540709542D75F4D33D10A00">Institute of Environmental Sciences, Plant-Microbial Interactions Group, Jagiellonian University, Gronostajowa 7, 30 - 387 Kraków, Poland</mods:affiliation>
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<mods:namePart id="6C4D243148ABBD574B6AB2708F49E910">Kozłowska, Anna</mods:namePart>
<mods:affiliation id="ACB4424A60C76EA4926911F7563FDAF9">Department of Ecology, Protection and Shaping of Environment, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71 - 434 Szczecin, Poland</mods:affiliation>
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<subSubSection id="C330A5C6FFFBFFA574E07C93CA71FDC9" pageId="5" pageNumber="246" type="nomenclature">
<paragraph id="8B95F64DFFFBFFA574E07C93C82EFDF7" blockId="5.[136,819,500,527]" box="[136,819,500,527]" pageId="5" pageNumber="246">
<heading id="D0DD4121FFFBFFA574E07C93C82EFDF7" box="[136,819,500,527]" fontSize="11" level="2" pageId="5" pageNumber="246" reason="3">
<taxonomicName id="4C2A8DCEFFFBFFA574E07C93C94EFDF7" ID-CoL="379BQ" authority="Blaszk. &amp; Kozlowska" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[136,595,500,527]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea" status="sp. nov.">
<emphasis id="B95E2A5FFFFBFFA574E07C93CA48FDF7" bold="true" box="[136,341,500,527]" italics="true" pageId="5" pageNumber="246">Dominikia litorea</emphasis>
Błaszk. &amp; Kozłowska
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,
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<emphasis id="B95E2A5FFFFBFFA576377C91C9B0FDF7" box="[607,685,502,527]" italics="true" pageId="5" pageNumber="246">sp. nov</emphasis>
.
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Fig. 3AH
</heading>
</paragraph>
<paragraph id="8B95F64DFFFBFFA574E07F7BCA71FDC9" blockId="5.[136,1410,540,597]" box="[136,364,540,561]" pageId="5" pageNumber="246">MycoBank MB 823832</paragraph>
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<paragraph id="8B95F64DFFFBFFA574E07F27CE9FFDAD" blockId="5.[136,1410,540,597]" box="[136,1410,576,597]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574E07F27CA7FFDAD" bold="true" box="[136,354,576,597]" pageId="5" pageNumber="246">Collections examined</emphasis>
:
<materialsCitation id="3B42FC10FFFBFFA575077F27CE9FFDAD" box="[367,1410,576,597]" collectionCode="ZT, Z, DEPSE, OSC" pageId="5" pageNumber="246" specimenCode="OSC 161511, OSC 161512" specimenCount="2" typeStatus="holotype">
<emphasis id="B95E2A5FFFFBFFA575077F27CAD1FDAD" bold="true" box="[367,460,576,597]" pageId="5" pageNumber="246">
<typeStatus id="549148EFFFFBFFA575077F27CAD1FDAD" box="[367,460,576,597]" pageId="5" pageNumber="246" type="holotype">Holotype</typeStatus>
</emphasis>
:—
<collectionCode id="ED3B6E88FFFBFFA575817F27C915FDAD" box="[489,520,576,597]" country="Switzerland" lsid="urn:lsid:biocol.org:col:15611" name="Eidgenössische Technische Hochschule Zürich" pageId="5" pageNumber="246" type="Herbarium">ZT</collectionCode>
Myc 58910 (
<collectionCode id="ED3B6E88FFFBFFA576E17F27C984FDAD" box="[649,665,576,597]" country="Switzerland" name="Universität Zürich" pageId="5" pageNumber="246" type="Herbarium">Z</collectionCode>
+
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),
<typeStatus id="549148EFFFFBFFA576BF7F27C834FDAD" box="[727,809,576,597]" pageId="5" pageNumber="246" type="isotype">isotypes</typeStatus>
: 36023614 (
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), and
<specimenCode id="DB8C5E36FFFBFFA5705A7F27CFADFDAD" box="[1074,1200,576,597]" collectionCode="OSC" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15613" lsid="urn:lsid:biocol.org:col:15613" name="Oregon State University" pageId="5" pageNumber="246" type="Herbarium">OSC 161511</specimenCode>
,
<specimenCode id="DB8C5E36FFFBFFA570D27F27CE2AFDAD" box="[1210,1335,576,597]" collectionCode="OSC" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15613" lsid="urn:lsid:biocol.org:col:15613" name="Oregon State University" pageId="5" pageNumber="246" type="Herbarium">OSC 161512</specimenCode>
(
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).
</materialsCitation>
</paragraph>
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<subSubSection id="C330A5C6FFFBFFA574E07FE1C8E1FD67" box="[136,1020,646,671]" pageId="5" pageNumber="246" type="etymology">
<paragraph id="8B95F64DFFFBFFA574E07FE1C8E1FD67" blockId="5.[136,1452,646,1823]" box="[136,1020,646,671]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574E07FE1CA14FD67" bold="true" box="[136,265,646,671]" pageId="5" pageNumber="246">Etymology</emphasis>
:—
<taxonomicName id="4C2A8DCEFFFBFFA575457FE1CA6AFD67" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[301,375,646,671]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA575457FE1CA6AFD67" box="[301,375,646,671]" italics="true" pageId="5" pageNumber="246">litorea</emphasis>
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, referring to the sandy dunes, in which the species exists.
</paragraph>
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<subSubSection id="C330A5C6FFFBFFA574D57FCDCF57FD1F" pageId="5" pageNumber="246" type="diagnosis">
<paragraph id="8B95F64DFFFBFFA574D57FCDCF57FD1F" blockId="5.[136,1452,646,1823]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574D57FCDCA2DFD3B" bold="true" box="[189,304,682,707]" pageId="5" pageNumber="246">Diagnosis</emphasis>
:—Differs from
<taxonomicName id="4C2A8DCEFFFBFFA575877FCDC946FD3B" box="[495,603,682,707]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="indica">
<emphasis id="B95E2A5FFFFBFFA575877FCDC946FD3B" box="[495,603,682,707]" italics="true" pageId="5" pageNumber="246">D. indica</emphasis>
</taxonomicName>
in the phenotypic and histochemical properties of spore wall layers, the morphological characters of the spore subtending hypha, and in molecular phylogeny.
</paragraph>
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<subSubSection id="C330A5C6FFFBFFA574D57F95CF10FA23" pageId="5" pageNumber="246" type="description">
<paragraph id="8B95F64DFFFBFFA574D57F95CBE5FB27" blockId="5.[136,1452,646,1823]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574D57F95CA5AFCF3" bold="true" box="[189,327,754,779]" pageId="5" pageNumber="246">Description</emphasis>
:—Spores formed in soil in loose to compact clusters of 322 spores; develop blastically at the tip of hyphae branched from a parent hypha continuous with a mycorrhizal extraradical hypha (Fig. 3AH); clusters frequently with incorporated soil debris (Fig. 3D, E).
<emphasis id="B95E2A5FFFFBFFA5768C7E5DC832FCAB" box="[740,815,826,851]" italics="true" pageId="5" pageNumber="246">Spores</emphasis>
hyaline; globose to subglobose; (11)25(35) μm diam; rarely egg-shaped or irregular, 2333 × 2950 μm; with one subtending hypha (Fig. 3AH).
<emphasis id="B95E2A5FFFFBFFA570E17E39CFE2FC8F" box="[1161,1279,862,887]" italics="true" pageId="5" pageNumber="246">Spore wall</emphasis>
consists of two layers (layers 1 and 2) of equal thickness, (0.8)1.0(1.2) μm thick (Fig. 3BH). Layer 1, forming the spore surface, permanent, unit (not divided into visible sublayers), smooth. Layer 2 permanent, laminate, smooth, consisting of very thin, &lt;0.5 μm, sublayers tightly adherent to each other and therefore difficult to see; this layer frequently separates from the lower surface of layer
<quantity id="4CD25BA8FFFBFFA5759C7E89C93DFBFF" box="[500,544,1006,1031]" metricMagnitude="-2" metricUnit="m" metricValue="2.54" pageId="5" pageNumber="246" unit="in" value="1.0">1 in</quantity>
crushed spores and uncrushed spores subjected to the pressure of a microscope cover slip (Fig. 3BG). In Melzers reagent, only layer 2 usually stains pale red (7A3) to pastel red (7A5), rarely high red (9A8), or turns pastel yellow (3A4; Fig. 3CH).
<emphasis id="B95E2A5FFFFBFFA576B67951C8B4FBB7" box="[734,937,1078,1103]" italics="true" pageId="5" pageNumber="246">Subtending hypha</emphasis>
hyaline; straight or recurved, usually funnel-shaped, more rarely cylindrical, rarely slightly constricted at the spore base; (2.5)3.7(9.0) μm wide at the spore base (Fig. 3CH).
<emphasis id="B95E2A5FFFFBFFA5754A7919C95FFB6F" box="[290,578,1150,1175]" italics="true" pageId="5" pageNumber="246">Wall of subtending hypha</emphasis>
hyaline; (0.8)1.3(2.0) μm thick at the spore base; composed of two layers continuous with spore wall layers 1 and 2 (Fig. 3G, H).
<emphasis id="B95E2A5FFFFBFFA5769879C5C838FB43" box="[752,805,1186,1211]" italics="true" pageId="5" pageNumber="246">Pore</emphasis>
(0.8)1.6(5.8) μm diam, open (Fig. 3FH).
<emphasis id="B95E2A5FFFFBFFA5717479C5CEB6FB43" box="[1308,1451,1186,1211]" italics="true" pageId="5" pageNumber="246">Germination</emphasis>
unknown.
</paragraph>
<paragraph id="8B95F64DFFFBFFA574D5798DCFE5FA97" blockId="5.[136,1452,646,1823]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574D5798DCAF0FAFB" bold="true" box="[189,493,1258,1283]" pageId="5" pageNumber="246">Mycorrhizal associations</emphasis>
:—As indicated studies of trap cultures, in the field,
<taxonomicName id="4C2A8DCEFFFBFFA5700D798DCFCBFAFB" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[1125,1238,1258,1283]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA5700D798DCFCBFAFB" box="[1125,1238,1258,1283]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
probably lived in mycorrhizal symbiosis with
<taxonomicName id="4C2A8DCEFFFBFFA575AE7869C952FADF" box="[454,591,1294,1319]" class="Magnoliopsida" family="Asteraceae" genus="Xanthium" kingdom="Plantae" order="Asterales" pageId="5" pageNumber="246" phylum="Tracheophyta" rank="species" species="spinosum">
<emphasis id="B95E2A5FFFFBFFA575AE7869C952FADF" box="[454,591,1294,1319]" italics="true" pageId="5" pageNumber="246">X. spinosum</emphasis>
</taxonomicName>
that had colonized sand dunes of the Mediterranean Sea located near Verico,
<collectingCountry id="F33DB6DDFFFBFFA574E07855CBC6FAB3" box="[136,219,1330,1355]" name="Greece" pageId="5" pageNumber="246">Greece</collectingCountry>
. However, spores of the fungus were not found in the field-collected rhizosphere soil of
<taxonomicName id="4C2A8DCEFFFBFFA570B57855CE48FAB3" box="[1245,1365,1330,1355]" class="Magnoliopsida" family="Asteraceae" genus="Xanthium" kingdom="Plantae" order="Asterales" pageId="5" pageNumber="246" phylum="Tracheophyta" rank="species" species="spinosa">
<emphasis id="B95E2A5FFFFBFFA570B57855CE48FAB3" box="[1245,1365,1330,1355]" italics="true" pageId="5" pageNumber="246">X. spinosa</emphasis>
</taxonomicName>
and no molecular analyses were performed to confirm the presence of
<taxonomicName id="4C2A8DCEFFFBFFA577237831C8AAFA97" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[843,951,1366,1391]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA577237831C8AAFA97" box="[843,951,1366,1391]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
in roots of the plant species.
</paragraph>
<paragraph id="8B95F64DFFFBFFA574D5781DCF10FA23" blockId="5.[136,1452,646,1823]" pageId="5" pageNumber="246">
In single-species cultures with
<taxonomicName id="4C2A8DCEFFFBFFA5767D781DC9B8FA6B" box="[533,677,1402,1427]" class="Magnoliopsida" family="Plantaginaceae" genus="Plantago" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="246" phylum="Tracheophyta" rank="species" species="lanceolata">
<emphasis id="B95E2A5FFFFBFFA5767D781DC9B8FA6B" box="[533,677,1402,1427]" italics="true" pageId="5" pageNumber="246">P. lanceolata</emphasis>
</taxonomicName>
as host plant,
<taxonomicName id="4C2A8DCEFFFBFFA5772B781DC8B3FA6B" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[835,942,1402,1427]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA5772B781DC8B3FA6B" box="[835,942,1402,1427]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
formed mycorrhiza with arbuscules, vesicles, and intra- and extraradical hyphae. All the structures were widely distributed along the root fragments examined and stained clearly [violet white (16A2) to deep violet (16E8)] in 0.1% Trypan Blue.
</paragraph>
</subSubSection>
<subSubSection id="C330A5C6FFFBFFA574D57881CE7BF8E7" pageId="5" pageNumber="246" type="distribution">
<paragraph id="8B95F64DFFFBFFA574D57881CE7BF8E7" blockId="5.[136,1452,646,1823]" pageId="5" pageNumber="246">
<emphasis id="B95E2A5FFFFBFFA574D57881CAF9FA07" bold="true" box="[189,484,1510,1535]" pageId="5" pageNumber="246">Distribution and habitat</emphasis>
:—Despite during the last 34 years J. Błaszkowski examined the occurrence of AMF in ca. 2500 field-collected soil samples and ca. 3000 trap cultures that represented different habitats, mainly maritime dunes, located in different regions of Africa, Asia, Europe,
<collectingCountry id="F33DB6DDFFFBFFA5775C7B49C865F9BF" box="[820,888,1582,1607]" name="Brazil" pageId="5" pageNumber="246">Brazil</collectingCountry>
and
<collectingCountry id="F33DB6DDFFFBFFA577DB7B49C8F3F9BF" box="[947,1006,1582,1607]" name="United States of America" pageId="5" pageNumber="246">USA</collectingCountry>
,
<taxonomicName id="4C2A8DCEFFFBFFA577947B49CF77F9BF" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[1020,1130,1582,1607]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA577947B49CF77F9BF" box="[1020,1130,1582,1607]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
was found only in one trap culture. The culture represented the
<collectingCountry id="F33DB6DDFFFBFFA5767B7B35C97FF993" box="[531,610,1618,1643]" name="Greece" pageId="5" pageNumber="246">Greece</collectingCountry>
sand dunes of the Mediterranean Sea located near Verico. However, BLAST searches indicated that
<taxonomicName id="4C2A8DCEFFFBFFA575F87B11CAE0F977" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[400,509,1654,1679]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA575F87B11CAE0F977" box="[400,509,1654,1679]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
has probably also been associated with roots of an unnamed plant species growing in
<collectingCountry id="F33DB6DDFFFBFFA574CE7BFDCBF0F94B" box="[166,237,1690,1715]" name="China" pageId="5" pageNumber="246">China</collectingCountry>
. The identity of the SSUITSLSU sequences of
<taxonomicName id="4C2A8DCEFFFBFFA577767BFDC896F94B" authorityName="Blaszk. &amp; Kozlowska" authorityYear="2018" box="[798,907,1690,1715]" class="Glomeromycetes" family="Glomeraceae" genus="Dominikia" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="litorea">
<emphasis id="B95E2A5FFFFBFFA577767BFDC896F94B" box="[798,907,1690,1715]" italics="true" pageId="5" pageNumber="246">D. litorea</emphasis>
</taxonomicName>
and two SSUITSLSU sequences (KF836940, KF836961) of the Chinese AMF named
<taxonomicName id="4C2A8DCEFFFBFFA5763D7BD9C9F1F92F" box="[597,748,1726,1751]" class="Glomeromycetes" family="Glomeraceae" genus="Glomus" kingdom="Fungi" order="Glomerales" pageId="5" pageNumber="246" phylum="Glomeromycota" rank="species" species="undefined-6">
<emphasis id="B95E2A5FFFFBFFA5763D7BD9C9B1F92F" box="[597,684,1726,1751]" italics="true" pageId="5" pageNumber="246">Glomus</emphasis>
sp. 6
</taxonomicName>
, which were obtained in so called environmental studies, was 98%. Of the RPB1 gene sequences deposited in public databases, the percent identity of none suggested a molecular conspecificity with our new species. The highest identity was only 92% and regarded, among others,
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<emphasis id="B95E2A5FFFFBFFA5709F7A61CE42F8E7" box="[1271,1375,1798,1823]" italics="true" pageId="5" pageNumber="246">D. indica</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>

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<paragraph id="8B952A30535DFF99D1FF6D22FDB2F8F7" blockId="10.[136,1451,1812,1911]" pageId="10" pageNumber="101">
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<emphasis id="B95EF622535DFF99D1FF6D22FC8AF88A" bold="true" box="[136,491,1812,1839]" italics="true" pageId="10" pageNumber="101">Neopestalotiopsis saprophytica</emphasis>
(Maharachch. &amp; K.D. Hyde) Maharachch., K.D. Hyde &amp; Crous,
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Stud. Mycol. 79: 148 (2014)
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MycoBank:
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809780, Faces of fungi number: FoF 13405,
<figureCitation id="131136B5535DFF99D2436D6AFEF2F8D3" box="[820,915,1884,1910]" captionStart="FIGURE 3" captionStartId="11.[136,229,1912,1934]" captionTargetBox="[291,1292,494,1885]" captionTargetId="figure-200@11.[291,1295,494,1885]" captionTargetPageId="11" captionText="FIGURE 3. Neopestalotiopsis saprophytica (MFLU 23-0396 (a, b), MFLU 23-0397 (c, d), MFLUCC 23-0259 (e, j, p), MFLUCC 23- 0260 (fh, kn, p) new host record). a, c Diseased leaves of Camellia sinensis var. assamica. b, d Close-up of lesions. e Upper and reverse views of the PDA (MFLUCC 23-0259) (at 25 °C). f Upper and reverse views of the PDA (MFLUCC 23-0260) (at 25 °C). On culture: gi Pycnidial conidiomata. j Mycelium. k, l Conidial attachment to conidiogenous cells. kl Conidiogenous cells. mp Conidia. Scale bars: h = 50 μm, jp = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13215610" httpUri="https://zenodo.org/record/13215610/files/figure.png" pageId="10" pageNumber="101">Figure 3</figureCitation>
</paragraph>
<paragraph id="8B952A30535DFF98D1FF6D92F9B4FE7D" blockId="10.[136,1452,1956,2091]" lastBlockId="11.[136,1452,158,473]" lastPageId="11" lastPageNumber="102" pageId="10" pageNumber="101">
Description:—Associated with lesions of the diseased leaves of
<taxonomicName id="4C2A51B3535DFF99D22C6D92F9A2F81B" box="[859,1219,1956,1983]" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" pageId="10" pageNumber="101" phylum="Tracheophyta" rank="variety" species="sinensis" variety="assamica">
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var.
<emphasis id="B95EF622535DFF99D52C6D93F9A2F81B" box="[1115,1219,1957,1982]" italics="true" pageId="10" pageNumber="101">assamica</emphasis>
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. Appearing as black spots on dead tissues of the leaf epidermis.
<emphasis id="B95EF622535DFF99D3F06DFEFE2BF847" bold="true" box="[647,842,1992,2018]" pageId="10" pageNumber="101">Asexual morph:</emphasis>
<emphasis id="B95EF622535DFF99D2236DFEFE87F847" box="[852,998,1992,2018]" italics="true" pageId="10" pageNumber="101">Conidiomata</emphasis>
pycnidial on
<collectionCode id="ED3BB2F5535DFF99D5FF6DFEF9A2F847" box="[1160,1219,1992,2018]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="10" pageNumber="101" type="Herbarium">PDA</collectionCode>
, 111475 μm diam. (
<emphasis id="B95EF622535DFF99D1E66DDBFDFFF7A3" box="[145,158,2029,2054]" italics="true" pageId="10" pageNumber="101"></emphasis>
= 293 μm), aggregated, mostly globose, reniform or oblong, exuding black slimy, glistening conidial masses.
<emphasis id="B95EF622535DFF99D1FF6226FC4FF78F" box="[136,302,2064,2090]" italics="true" pageId="10" pageNumber="101">Conidiophores</emphasis>
unbranched, aseptate, often reduced to conidiogenous cell.
<emphasis id="B95EF622535DFF99D2B26226F9C9F78F" box="[965,1192,2064,2090]" italics="true" pageId="10" pageNumber="101">Conidiogenous cells</emphasis>
926 × 38 μm (
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= 17 × 5 μm,
<emphasis id="B95EF622535CFF98D1916AA9FD95FF1D" box="[230,244,159,184]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20), discrete, annellidic, clavate, oblong-cylindrical, hyaline to pale yellow, smooth, thin-walled.
<emphasis id="B95EF622535CFF98D4266AA8F8CAFF1D" box="[1361,1451,158,184]" italics="true" pageId="11" pageNumber="102">Conidia</emphasis>
19.450.9 × 4.612.6 μm (
<emphasis id="B95EF622535CFF98D0C46AF5FCDEFF79" box="[435,447,195,220]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 29.2× 6.6 μm,
<emphasis id="B95EF622535CFF98D3F76AF5FFEFFF79" box="[640,654,195,220]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20), fusoid to fusiform, straight to slightly curved, 4-septate, initially hyaline to pale yellow, later turning to olivaceous, smooth, thin-walled at maturity; basal cell 16 μm long (
<emphasis id="B95EF622535CFF98D4596AD1F85AFEA5" box="[1326,1339,231,256]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 4.9 μm,
<emphasis id="B95EF622535CFF98D1FF6B3DFDF7FE81" box="[136,150,267,292]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20), acute conic, hyaline, infrequently light yellow, thin-walled, smooth; three median cells each 2.57.6 μm long (
<emphasis id="B95EF622535CFF98D1E66B19FDFFFEED" box="[145,158,303,328]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 4.9 μm,
<emphasis id="B95EF622535CFF98D06D6B19FC49FEED" box="[282,296,303,328]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20), doliiform, olivaceous, concolorous, verruculose, periclinal walls darker than the rest of the cell; second cell from base 4.47.6 μm long; third cell 3.56.4 μm long; fourth cell 2.56.3 μm long; apical cell 1.85.0 μm long (
<emphasis id="B95EF622535CFF98D1806B41FC62FE35" box="[247,259,375,400]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 3.5 μm,
<emphasis id="B95EF622535CFF98D0F76B41FCEFFE35" box="[384,398,375,400]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20), hyaline, subcylindrical to obconic, with 24 (often 3) tubular appendages arising from the apex of the apical cell, unbranched, flexuous, filiform, 4.129.9 μm (
<emphasis id="B95EF622535CFF98D2C56BADFEDFFE11" box="[946,958,411,436]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 13.8 μm,
<emphasis id="B95EF622535CFF98D5326BADF932FE11" box="[1093,1107,411,436]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20); basal appendage single, tubular, unbranched, centric, 2.311.2 μm long, (
<emphasis id="B95EF622535CFF98D3D96B89FFDBFE7D" box="[686,698,447,472]" italics="true" pageId="11" pageNumber="102"></emphasis>
= 3 μm,
<emphasis id="B95EF622535CFF98D2576B89FE4FFE7D" box="[800,814,447,472]" italics="true" pageId="11" pageNumber="102">n</emphasis>
= 20).
<emphasis id="B95EF622535CFF98D2086B88F951FE7D" bold="true" box="[895,1072,446,472]" pageId="11" pageNumber="102">Sexual morph:</emphasis>
Not observed.
</paragraph>
</subSubSection>
<caption id="DF557AB8535CFF98D1FF6D4EFC03F7BB" ID-DOI="http://doi.org/10.5281/zenodo.13215610" ID-Zenodo-Dep="13215610" httpUri="https://zenodo.org/record/13215610/files/figure.png" pageId="11" pageNumber="102" startId="11.[136,229,1912,1934]" targetBox="[291,1292,494,1885]" targetPageId="11" targetType="figure">
<paragraph id="8B952A30535CFF98D1FF6D4EFC03F7BB" blockId="11.[136,1451,1912,2079]" pageId="11" pageNumber="102">
<emphasis id="B95EF622535CFF98D1FF6D4EFD98F828" bold="true" box="[136,249,1912,1934]" pageId="11" pageNumber="102">FIGURE 3</emphasis>
.
<taxonomicName id="4C2A51B3535CFF98D0716D4FFF54F82B" box="[262,565,1913,1934]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="11" pageNumber="102" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535CFF98D0716D4FFF54F82B" box="[262,565,1913,1934]" italics="true" pageId="11" pageNumber="102">Neopestalotiopsis saprophytica</emphasis>
</taxonomicName>
(MFLU 23-0396 (a, b), MFLU 23-0397 (c, d), MFLUCC 23-0259 (e, j, p), MFLUCC 23- 0260 (fh, kn, p)
<emphasis id="B95EF622535CFF98D04F6DABFCBBF817" bold="true" box="[312,474,1949,1970]" pageId="11" pageNumber="102">new host record</emphasis>
). a, c Diseased leaves of
<taxonomicName id="4C2A51B3535CFF98D3BF6DABFE99F817" box="[712,1016,1949,1970]" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" pageId="11" pageNumber="102" phylum="Tracheophyta" rank="variety" species="sinensis" variety="assamica">
<emphasis id="B95EF622535CFF98D3BF6DABFE10F817" box="[712,881,1949,1970]" italics="true" pageId="11" pageNumber="102">Camellia sinensis</emphasis>
var.
<emphasis id="B95EF622535CFF98D2E86DABFE99F817" box="[927,1016,1949,1970]" italics="true" pageId="11" pageNumber="102">assamica</emphasis>
</taxonomicName>
. b, d Close-up of lesions. e Upper and reverse views of the PDA (MFLUCC 23-0259) (at 25 °C). f Upper and reverse views of the PDA (MFLUCC 23-0260) (at 25 °C). On culture: gi Pycnidial conidiomata. j Mycelium. k, l Conidial attachment to conidiogenous cells. kl Conidiogenous cells. mp Conidia. Scale bars: h = 50 μm, jp = 20 μm.
</paragraph>
</caption>
<subSubSection id="C33079BB535BFF9FD1CA6AA8FD95FE7D" pageId="12" pageNumber="103" type="biology_ecology">
<paragraph id="8B952A30535BFF9FD1CA6AA8FEADFEA5" blockId="12.[136,1452,158,1301]" pageId="12" pageNumber="103">
Culture characteristics:—Colonies on
<collectionCode id="ED3BB2F5535BFF9FD31F6AA8FFC1FF1D" box="[616,672,158,184]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="12" pageNumber="103" type="Herbarium">PDA</collectionCode>
filamentous to circular, slightly undulate to filiform margin, attaining
<quantity id="4CD287D5535BFF9FD1FF6AF4FC61FF78" box="[136,256,194,221]" metricMagnitude="-2" metricUnit="m" metricValue="3.5" metricValueMax="4.0" metricValueMin="3.0" pageId="12" pageNumber="103" unit="mm" value="35.0" valueMax="40.0" valueMin="30.0">3040 mm</quantity>
diam. after 7 days at 25 °
<collectionCode id="ED3BB2F5535BFF9FD36E6AF4FF4EFF79" box="[537,559,194,220]" country="Denmark" name="University of Copenhagen" pageId="12" pageNumber="103" type="Herbarium">C</collectionCode>
, surface white, cottony, aerial mycelium on surface, flat elevation, reverse white to pale yellowish. Sporulates after 10 days; conidiomata black, gregarious.
</paragraph>
<paragraph id="8B952A30535BFF9FD1CA6B3DFD95FE7D" blockId="12.[136,1452,158,1301]" pageId="12" pageNumber="103">
Known hosts:—Saprobic on leaves of
<taxonomicName id="4C2A51B3535BFF9FD3E36B3CFE51FE80" box="[660,816,266,293]" class="Magnoliopsida" family="Magnoliaceae" genus="Magnolia" kingdom="Plantae" order="Magnoliales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="undetermined">
<emphasis id="B95EF622535BFF9FD3E36B3CFE60FE81" box="[660,769,266,292]" italics="true" pageId="12" pageNumber="103">Magnolia</emphasis>
sp.
</taxonomicName>
(
<taxonomicName id="4C2A51B3535BFF9FD23F6B3CFE89FE81" authorityName="de Jussieu" authorityYear="1789" box="[840,1000,266,292]" class="Magnoliopsida" family="Magnoliaceae" kingdom="Plantae" order="Magnoliales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="family">
<emphasis id="B95EF622535BFF9FD23F6B3CFE89FE81" box="[840,1000,266,292]" italics="true" pageId="12" pageNumber="103">Magnoliaceae</emphasis>
</taxonomicName>
) and on fruits of
<taxonomicName id="4C2A51B3535BFF9FD5A56B3DF8CDFE81" box="[1234,1452,266,292]" class="Magnoliopsida" family="Lauraceae" genus="Litsea" kingdom="Plantae" order="Laurales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="rotundifolia">
<emphasis id="B95EF622535BFF9FD5A56B3DF8CDFE81" box="[1234,1452,266,292]" italics="true" pageId="12" pageNumber="103">Litsea rotundifolia</emphasis>
</taxonomicName>
(
<bibRefCitation id="EFBB57C1535BFF9FD1F86B18FF70FEED" author="Maharachchikumbura, S. S. N. &amp; Guo, L. D. &amp; Cai, L. &amp; Chukeatirote, E. &amp; Wu, W. P. &amp; Sun, X. &amp; Crous, P. W. &amp; Bhat, D. J. &amp; McKenzie, E. H. &amp; Bahkali, A. H. &amp; Hyde, K. D." box="[143,529,302,329]" pageId="12" pageNumber="103" pagination="95 - 129" refId="ref14892" refString="Maharachchikumbura, S. S. N., Guo, L. D., Cai, L., Chukeatirote, E., Wu, W. P., Sun, X., Crous, P. W., Bhat, D. J., McKenzie, E. H., Bahkali, A. H. &amp; Hyde, K. D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95 - 129. https: // doi. org / 10.1007 / s 13225 - 012 - 0198 - 1" type="journal article" year="2012">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD0E66B19FCA4FEED" box="[401,453,302,328]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2012
</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD3686B18FF3DFEED" author="Maharachchikumbura, S. S. N. &amp; Hyde, K. D. &amp; Groenewald, J. Z. &amp; Xu, J. &amp; Crous, P. W." box="[543,604,302,328]" pageId="12" pageNumber="103" pagination="121 - 186" refId="ref15008" refString="Maharachchikumbura, S. S. N., Hyde, K. D., Groenewald, J. Z., Xu, J. and Crous, P. W. (2014) Pestalotiopsis revisited. Studies in Mycology 79: 121 - 186. https: // doi. org / 10.1016 / j. simyco. 2014.09.005" type="journal article" year="2014">2014</bibRefCitation>
); pathogenic causing leaf spots on Oil palm (
<taxonomicName id="4C2A51B3535BFF9FD5196B18FD90FEC9" authority="(Ismail et al. 2017)" baseAuthorityName="Ismail" baseAuthorityYear="2017" class="Liliopsida" family="Arecaceae" genus="Elaeis" kingdom="Plantae" order="Arecales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="guineensis">
<emphasis id="B95EF622535BFF9FD5196B18F855FEED" box="[1134,1332,302,328]" italics="true" pageId="12" pageNumber="103">Elaeis guineensis</emphasis>
(
<bibRefCitation id="EFBB57C1535BFF9FD4326B18FD88FEC9" author="Ismail, S. I. &amp; Zulperi, D. &amp; Norddin, S. &amp; Ahmad-Hamdani, S." pageId="12" pageNumber="103" refId="ref13617" refString="Ismail, S. I., Zulperi, D., Norddin, S. &amp; Ahmad-Hamdani, S. (2017) First report of Neopestalotiopsis saprophytica causing leaf spot of oil palm (Elaeis guineensis) in Malaysia. Plant Disease 101: e 1821. https: // doi. org / 10.1094 / PDIS- 02 - 17 - 0271 - PDN" type="journal volume" year="2017">
Ismail
<emphasis id="B95EF622535BFF9FD4E06B19FDFFFEC9" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2017
</bibRefCitation>
)
</taxonomicName>
,
<taxonomicName id="4C2A51B3535BFF9FD1896B64FE65FEC9" authority="(Qin et al. 2020)" baseAuthorityName="Qin" baseAuthorityYear="2020" box="[254,772,338,365]" class="Liliopsida" family="Orchidaceae" genus="Paphiopedilum" kingdom="Plantae" order="Asparagales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="micranthum">
<emphasis id="B95EF622535BFF9FD1896B64FF58FEC9" box="[254,569,338,364]" italics="true" pageId="12" pageNumber="103">Paphiopedilum micranthum</emphasis>
(
<bibRefCitation id="EFBB57C1535BFF9FD33E6B64FF9DFEC9" author="Qin, Q. &amp; Lu, Z. &amp; Lu, Z. &amp; Ding, L. &amp; Chi, Z. &amp; Shan, B." box="[585,764,338,365]" pageId="12" pageNumber="103" refId="ref16304" refString="Qin, Q., Lu, Z., Lu, Z., Ding, L., Chi, Z. &amp; Shan, B. (2020) First report of leaf spot on Paphiopedilum micranthum caused by Neopestalotiopsis saprophytica in China. Plant Disease 104: e 2738. https: // doi. org / 10.1094 / PDIS- 02 - 20 - 0275 - PDN" type="journal volume" year="2020">
Qin
<emphasis id="B95EF622535BFF9FD3096B65FFD1FEC9" box="[638,688,338,364]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2020
</bibRefCitation>
)
</taxonomicName>
, and
<taxonomicName id="4C2A51B3535BFF9FD2366B64F84BFEC9" authority="(Yang et al. 2021)" baseAuthorityName="Yang" baseAuthorityYear="2021" box="[833,1322,338,364]" class="Magnoliopsida" family="Erythropalaceae" genus="Erythropalum" kingdom="Plantae" order="Santalales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="scandens">
<emphasis id="B95EF622535BFF9FD2366B64F92FFEC9" box="[833,1102,338,364]" italics="true" pageId="12" pageNumber="103">Erythropalum scandens</emphasis>
(
<bibRefCitation id="EFBB57C1535BFF9FD5296B64F842FEC9" author="Yang, Q. &amp; Zeng, X. Y. &amp; Yuan, J. &amp; Zhang, Q. &amp; He, Y. K. &amp; Wang, Y." box="[1118,1315,338,364]" pageId="12" pageNumber="103" refId="ref18042" refString="Yang, Q., Zeng, X. Y., Yuan, J., Zhang, Q., He, Y. K. &amp; Wang, Y. (2021) Two new species of Neopestalotiopsis from southern China. Biodiversity Data Journal 9: e 70446. https: // doi. org / 10.3897 / BDJ. 9. e 70446" type="journal volume" year="2021">
Yang
<emphasis id="B95EF622535BFF9FD5D26B65F9BFFEC9" box="[1189,1246,338,364]" italics="true" pageId="12" pageNumber="103">et al.</emphasis>
2021
</bibRefCitation>
)
</taxonomicName>
, leaf blight on
<taxonomicName id="4C2A51B3535BFF9FD1DA6B41FF2CFE35" authority="(Ismail et al. 2022)" baseAuthorityName="Ismail" baseAuthorityYear="2022" box="[173,589,374,401]" class="Liliopsida" family="Arecaceae" genus="Roystonea" kingdom="Plantae" order="Arecales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="regia">
<emphasis id="B95EF622535BFF9FD1DA6B41FC04FE35" box="[173,357,375,400]" italics="true" pageId="12" pageNumber="103">Roystonea regia</emphasis>
(
<bibRefCitation id="EFBB57C1535BFF9FD0016B40FF24FE35" author="Ismail, S. I. &amp; Zulperi, D. &amp; Jamian, S. &amp; Mohd Hata, E." box="[374,581,374,401]" pageId="12" pageNumber="103" pagination="869 - 870" refId="ref13683" refString="Ismail, S. I., Zulperi, D., Jamian, S. &amp; Mohd Hata, E. (2022) First report of Neopestalotiopsis saprophytica causing leaf blight on royal palm in Malaysia. Journal of Plant Pathology 104: 869 - 870. https: // doi. org / 10.1094 / PDIS- 02 - 17 - 0271 - PDN" type="journal article" year="2022">
Ismail
<emphasis id="B95EF622535BFF9FD0B16B41FC98FE35" box="[454,505,374,400]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2022
</bibRefCitation>
)
</taxonomicName>
, fruit rot on Persimmon (
<taxonomicName id="4C2A51B3535BFF9FD2066B41F97DFE35" box="[881,1052,374,400]" class="Magnoliopsida" family="Ebenaceae" genus="Diospyros" kingdom="Plantae" order="Ericales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="kaki">
<emphasis id="B95EF622535BFF9FD2066B41F97DFE35" box="[881,1052,374,400]" italics="true" pageId="12" pageNumber="103">Diospyros kaki</emphasis>
</taxonomicName>
) (
<bibRefCitation id="EFBB57C1535BFF9FD5416B40F988FE35" author="Qin, R. &amp; Li, Q. &amp; Huang, S. &amp; Chen, X. &amp; Mo, J. &amp; Guo, T. &amp; Huang, H. &amp; Tang, L. &amp; Yu, Z." box="[1078,1257,374,401]" pageId="12" pageNumber="103" refId="ref16375" refString="Qin, R., Li, Q., Huang, S., Chen, X., Mo, J., Guo, T., Huang, H., Tang, L. &amp; Yu, Z., (2023) Fruit rot on persimmon caused by Neopestalotiopsis saprophytica and Neopestalotiopsis ellipsospora in Guangxi, China. Plant Disease 107: e 2531. https: // doi. org / 10.1094 / PDIS- 05 - 22 - 1168 - PDN" type="journal volume" year="2023">
Qin
<emphasis id="B95EF622535BFF9FD51D6B41F9FCFE35" box="[1130,1181,374,400]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2023
</bibRefCitation>
), associated with diseased leaves of
<taxonomicName id="4C2A51B3535BFF9FD02D6BACFFA3FE11" box="[346,706,410,437]" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="variety" species="sinensis" variety="assamica">
<emphasis id="B95EF622535BFF9FD02D6BACFF40FE11" box="[346,545,410,436]" italics="true" pageId="12" pageNumber="103">Camellia sinensis</emphasis>
var.
<emphasis id="B95EF622535BFF9FD32D6BADFFA3FE11" box="[602,706,411,436]" italics="true" pageId="12" pageNumber="103">assamica</emphasis>
</taxonomicName>
(this study); endophytic on leaves of
<taxonomicName id="4C2A51B3535BFF9FD51D6BACFD8FFE7D" authority="(Reddy et al. 2016)" baseAuthorityName="Reddy" baseAuthorityYear="2016" class="Magnoliopsida" family="Cordiaceae" genus="Cordia" kingdom="Plantae" order="Boraginales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="dichotoma">
<emphasis id="B95EF622535BFF9FD51D6BACF857FE11" box="[1130,1334,410,436]" italics="true" pageId="12" pageNumber="103">Cordia dichotoma</emphasis>
(
<bibRefCitation id="EFBB57C1535BFF9FD4326BACFD87FE7D" author="Reddy, M. S. &amp; Murali, T. S. &amp; Suryanarayanan, T. S. &amp; Rajulu, M. G. &amp; Thirunavukkarasu, N." pageId="12" pageNumber="103" pagination="70 - 75" refId="ref16628" refString="Reddy, M. S., Murali, T. S., Suryanarayanan, T. S., Rajulu, M. G. &amp; Thirunavukkarasu, N. (2016) Pestalotiopsis species occur as generalist endophytes in trees of Western Ghats forests of southern India. Fungal Ecology 24: 70 - 75. https: // doi. org / 10.1016 / j. funeco. 2016.09.002" type="journal article" year="2016">
Reddy
<emphasis id="B95EF622535BFF9FD4E06BADFDFFFE7D" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2016
</bibRefCitation>
)
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection id="C33079BB535BFF9FD1CA6BD5F958FD85" pageId="12" pageNumber="103" type="distribution">
<paragraph id="8B952A30535BFF9FD1CA6BD5F958FD85" blockId="12.[136,1452,158,1301]" pageId="12" pageNumber="103">
Known distribution:—
<collectingCountry id="F33D6AA0535BFF9FD0CB6BD4FF61FE59" box="[444,512,482,508]" name="China" pageId="12" pageNumber="103">China</collectingCountry>
(
<bibRefCitation id="EFBB57C1535BFF9FD3786BD4FEEBFE59" author="Maharachchikumbura, S. S. N. &amp; Guo, L. D. &amp; Cai, L. &amp; Chukeatirote, E. &amp; Wu, W. P. &amp; Sun, X. &amp; Crous, P. W. &amp; Bhat, D. J. &amp; McKenzie, E. H. &amp; Bahkali, A. H. &amp; Hyde, K. D." box="[527,906,482,509]" pageId="12" pageNumber="103" pagination="95 - 129" refId="ref14892" refString="Maharachchikumbura, S. S. N., Guo, L. D., Cai, L., Chukeatirote, E., Wu, W. P., Sun, X., Crous, P. W., Bhat, D. J., McKenzie, E. H., Bahkali, A. H. &amp; Hyde, K. D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95 - 129. https: // doi. org / 10.1007 / s 13225 - 012 - 0198 - 1" type="journal article" year="2012">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD2796BD5FE21FE59" box="[782,832,482,508]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2012
</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD2E16BD4FEB0FE59" author="Maharachchikumbura, S. S. N. &amp; Hyde, K. D. &amp; Groenewald, J. Z. &amp; Xu, J. &amp; Crous, P. W." box="[918,977,482,508]" pageId="12" pageNumber="103" pagination="121 - 186" refId="ref15008" refString="Maharachchikumbura, S. S. N., Hyde, K. D., Groenewald, J. Z., Xu, J. and Crous, P. W. (2014) Pestalotiopsis revisited. Studies in Mycology 79: 121 - 186. https: // doi. org / 10.1016 / j. simyco. 2014.09.005" type="journal article" year="2014">2014</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD2AA6BD4F9EAFE59" author="Qin, Q. &amp; Lu, Z. &amp; Lu, Z. &amp; Ding, L. &amp; Chi, Z. &amp; Shan, B." box="[989,1163,482,509]" pageId="12" pageNumber="103" refId="ref16304" refString="Qin, Q., Lu, Z., Lu, Z., Ding, L., Chi, Z. &amp; Shan, B. (2020) First report of leaf spot on Paphiopedilum micranthum caused by Neopestalotiopsis saprophytica in China. Plant Disease 104: e 2738. https: // doi. org / 10.1094 / PDIS- 02 - 20 - 0275 - PDN" type="journal volume" year="2020">
Qin
<emphasis id="B95EF622535BFF9FD5786BD5F920FE59" box="[1039,1089,482,508]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2020
</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD5E06BD4F9B3FE59" author="Qin, R. &amp; Li, Q. &amp; Huang, S. &amp; Chen, X. &amp; Mo, J. &amp; Guo, T. &amp; Huang, H. &amp; Tang, L. &amp; Yu, Z." box="[1175,1234,482,508]" pageId="12" pageNumber="103" refId="ref16375" refString="Qin, R., Li, Q., Huang, S., Chen, X., Mo, J., Guo, T., Huang, H., Tang, L. &amp; Yu, Z., (2023) Fruit rot on persimmon caused by Neopestalotiopsis saprophytica and Neopestalotiopsis ellipsospora in Guangxi, China. Plant Disease 107: e 2531. https: // doi. org / 10.1094 / PDIS- 05 - 22 - 1168 - PDN" type="journal volume" year="2023">2023</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD5A96BD5F8FEFE59" author="Yang, Q. &amp; Zeng, X. Y. &amp; Yuan, J. &amp; Zhang, Q. &amp; He, Y. K. &amp; Wang, Y." box="[1246,1439,482,509]" pageId="12" pageNumber="103" refId="ref18042" refString="Yang, Q., Zeng, X. Y., Yuan, J., Zhang, Q., He, Y. K. &amp; Wang, Y. (2021) Two new species of Neopestalotiopsis from southern China. Biodiversity Data Journal 9: e 70446. https: // doi. org / 10.3897 / BDJ. 9. e 70446" type="journal volume" year="2021">
Yang
<emphasis id="B95EF622535BFF9FD4556BD5F835FE59" box="[1314,1364,482,508]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2021
</bibRefCitation>
),
<collectingCountry id="F33D6AA0535BFF9FD1FF6830FD8EFD85" box="[136,239,518,544]" name="Malaysia" pageId="12" pageNumber="103">Malaysia</collectingCountry>
(
<bibRefCitation id="EFBB57C1535BFF9FD18A6830FCA7FD85" author="Ismail, S. I. &amp; Zulperi, D. &amp; Norddin, S. &amp; Ahmad-Hamdani, S." box="[253,454,518,545]" pageId="12" pageNumber="103" refId="ref13617" refString="Ismail, S. I., Zulperi, D., Norddin, S. &amp; Ahmad-Hamdani, S. (2017) First report of Neopestalotiopsis saprophytica causing leaf spot of oil palm (Elaeis guineensis) in Malaysia. Plant Disease 101: e 1821. https: // doi. org / 10.1094 / PDIS- 02 - 17 - 0271 - PDN" type="journal volume" year="2017">
Ismail
<emphasis id="B95EF622535BFF9FD03B6831FC1CFD85" box="[332,381,518,544]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2017
</bibRefCitation>
,
<bibRefCitation id="EFBB57C1535BFF9FD0A66830FF6CFD85" author="Ismail, S. I. &amp; Zulperi, D. &amp; Jamian, S. &amp; Mohd Hata, E." box="[465,525,518,544]" pageId="12" pageNumber="103" pagination="869 - 870" refId="ref13683" refString="Ismail, S. I., Zulperi, D., Jamian, S. &amp; Mohd Hata, E. (2022) First report of Neopestalotiopsis saprophytica causing leaf blight on royal palm in Malaysia. Journal of Plant Pathology 104: 869 - 870. https: // doi. org / 10.1094 / PDIS- 02 - 17 - 0271 - PDN" type="journal article" year="2022">2022</bibRefCitation>
),
<collectingCountry id="F33D6AA0535BFF9FD3576830FF3BFD85" box="[544,602,518,544]" name="India" pageId="12" pageNumber="103">India</collectingCountry>
(
<bibRefCitation id="EFBB57C1535BFF9FD31F6830FE54FD85" author="Reddy, M. S. &amp; Murali, T. S. &amp; Suryanarayanan, T. S. &amp; Rajulu, M. G. &amp; Thirunavukkarasu, N." box="[616,821,518,545]" pageId="12" pageNumber="103" pagination="70 - 75" refId="ref16628" refString="Reddy, M. S., Murali, T. S., Suryanarayanan, T. S., Rajulu, M. G. &amp; Thirunavukkarasu, N. (2016) Pestalotiopsis species occur as generalist endophytes in trees of Western Ghats forests of southern India. Fungal Ecology 24: 70 - 75. https: // doi. org / 10.1016 / j. funeco. 2016.09.002" type="journal article" year="2016">
Reddy
<emphasis id="B95EF622535BFF9FD3CD6831FF8AFD85" box="[698,747,518,544]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2016
</bibRefCitation>
),
<collectingCountry id="F33D6AA0535BFF9FD23E6830FECCFD85" box="[841,941,518,544]" name="Thailand" pageId="12" pageNumber="103">Thailand</collectingCountry>
(this study).
</paragraph>
</subSubSection>
<subSubSection id="C33079BB535BFF9FD1CA681CFCDDFD15" pageId="12" pageNumber="103" type="materials_examined">
<paragraph id="8B952A30535BFF9FD1CA681CFCDDFD15" blockId="12.[136,1452,158,1301]" pageId="12" pageNumber="103">
Materials examined:—
<collectingCountry id="F33D6AA0535BFF9FD0B3681CFF4BFDE1" box="[452,554,554,580]" name="Thailand" pageId="12" pageNumber="103">Thailand</collectingCountry>
,
<collectingRegion id="49EEE4D2535BFF9FD34E681CFE54FDE1" box="[569,821,554,580]" country="Thailand" name="Chiang Mai" pageId="12" pageNumber="103">Chiang Mai Province</collectingRegion>
, Mae Taeng District, Ban Pang Makluay Community Forest, alt.
<quantity id="4CD287D5535BFF9FD0786878FC3CFDCC" box="[271,349,590,617]" metricMagnitude="2" metricUnit="m" metricValue="5.3" pageId="12" pageNumber="103" unit="m" value="530.0">530 m</quantity>
, on symptomatic leaf of
<taxonomicName id="4C2A51B3535BFF9FD3FE6878FE9FFDCD" box="[649,1022,590,617]" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="variety" species="sinensis" variety="assamica">
<emphasis id="B95EF622535BFF9FD3FE6878FE34FDCD" box="[649,853,590,616]" italics="true" pageId="12" pageNumber="103">Camellia sinensis</emphasis>
var.
<emphasis id="B95EF622535BFF9FD2E16879FE9FFDCD" box="[918,1022,591,616]" italics="true" pageId="12" pageNumber="103">assamica</emphasis>
</taxonomicName>
(
<taxonomicName id="4C2A51B3535BFF9FD5646878F91FFDCD" box="[1043,1150,590,616]" class="Magnoliopsida" family="Theaceae" kingdom="Plantae" order="Ericales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="family">
<emphasis id="B95EF622535BFF9FD5646878F91FFDCD" box="[1043,1150,590,616]" italics="true" pageId="12" pageNumber="103">Theaceae</emphasis>
</taxonomicName>
),
<date id="FF940CF0535BFF9FD5EC6878F8E0FDCD" box="[1179,1409,590,616]" pageId="12" pageNumber="103" value="2022-09-28">28 September 2022</date>
,
<collectionCode id="ED3BB2F5535BFF9FD4E76879F8C6FDCC" box="[1424,1447,591,617]" country="Canada" lsid="urn:lsid:biocol.org:col:13946" name="Royal British Columbia Museum - Herbarium" pageId="12" pageNumber="103" type="Museum">V</collectionCode>
. Koodalugodaarchchi P6 (
<collectionCode id="ED3BB2F5535BFF9FD0DB6844FC9DFD29" box="[428,508,626,652]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="12" pageNumber="103">MFLU</collectionCode>
23-0396), living culture
<collectionCode id="ED3BB2F5535BFF9FD2526844FEF9FD29" box="[805,920,626,652]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0259;
<emphasis id="B95EF622535BFF9FD5636844F921FD29" box="[1044,1088,626,652]" italics="true" pageId="12" pageNumber="103">ibid</emphasis>
., P11 (
<collectionCode id="ED3BB2F5535BFF9FD5E16844F987FD29" box="[1174,1254,626,652]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="12" pageNumber="103">MFLU</collectionCode>
23-0397), living culture
<collectionCode id="ED3BB2F5535BFF9FD1AA68A0FC31FD15" box="[221,336,662,688]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0260.
</paragraph>
</subSubSection>
<subSubSection id="C33079BB535BFF9FD1CA688DFC78FAB1" pageId="12" pageNumber="103" type="discussion">
<paragraph id="8B952A30535BFF9FD1CA688DFC78FAB1" blockId="12.[136,1452,158,1301]" pageId="12" pageNumber="103">
Notes:—
<taxonomicName id="4C2A51B3535BFF9FD055688CFFE2FD71" box="[290,643,698,724]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD055688CFFE2FD71" box="[290,643,698,724]" italics="true" pageId="12" pageNumber="103">Neopestalotiopsis saprophytica</emphasis>
</taxonomicName>
was introduced by
<bibRefCitation id="EFBB57C1535BFF9FD217688CF989FD71" author="Maharachchikumbura, S. S. N. &amp; Guo, L. D. &amp; Cai, L. &amp; Chukeatirote, E. &amp; Wu, W. P. &amp; Sun, X. &amp; Crous, P. W. &amp; Bhat, D. J. &amp; McKenzie, E. H. &amp; Bahkali, A. H. &amp; Hyde, K. D." box="[864,1256,698,725]" pageId="12" pageNumber="103" pagination="95 - 129" refId="ref14892" refString="Maharachchikumbura, S. S. N., Guo, L. D., Cai, L., Chukeatirote, E., Wu, W. P., Sun, X., Crous, P. W., Bhat, D. J., McKenzie, E. H., Bahkali, A. H. &amp; Hyde, K. D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95 - 129. https: // doi. org / 10.1007 / s 13225 - 012 - 0198 - 1" type="journal article" year="2012">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD52A688DF9EEFD71" box="[1117,1167,698,724]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. (2012)
</bibRefCitation>
as
<taxonomicName id="4C2A51B3535BFF9FD479688CFFCAFD5D" authority="Maharachchikumbura &amp; K. D. Hyde" authorityName="Maharachchikumbura &amp; K. D. Hyde" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophyta">
<emphasis id="B95EF622535BFF9FD479688CFC67FD5D" italics="true" pageId="12" pageNumber="103">Pestalotiopsis saprophyta</emphasis>
Maharachchikumbura &amp; K.D. Hyde
</taxonomicName>
, a saprobe on the leaves of
<taxonomicName id="4C2A51B3535BFF9FD29968E8F9E4FD5C" box="[1006,1157,734,761]" class="Magnoliopsida" family="Magnoliaceae" genus="Magnolia" kingdom="Plantae" order="Magnoliales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="species" species="undetermined">
<emphasis id="B95EF622535BFF9FD29968E8F93AFD5D" box="[1006,1115,734,760]" italics="true" pageId="12" pageNumber="103">Magnolia</emphasis>
sp.
</taxonomicName>
,
<collectingRegion id="49EEE4D2535BFF9FD5E468E9F83AFD5D" box="[1171,1371,734,761]" country="China" name="Yunnan" pageId="12" pageNumber="103">Yunnan Province</collectingRegion>
,
<collectingCountry id="F33D6AA0535BFF9FD41F68E8F8CAFD5D" box="[1384,1451,734,760]" name="China" pageId="12" pageNumber="103">China</collectingCountry>
(
<bibRefCitation id="EFBB57C1535BFF9FD1F86934FF76FCB9" author="Maharachchikumbura, S. S. N. &amp; Guo, L. D. &amp; Cai, L. &amp; Chukeatirote, E. &amp; Wu, W. P. &amp; Sun, X. &amp; Crous, P. W. &amp; Bhat, D. J. &amp; McKenzie, E. H. &amp; Bahkali, A. H. &amp; Hyde, K. D." box="[143,535,770,797]" pageId="12" pageNumber="103" pagination="95 - 129" refId="ref14892" refString="Maharachchikumbura, S. S. N., Guo, L. D., Cai, L., Chukeatirote, E., Wu, W. P., Sun, X., Crous, P. W., Bhat, D. J., McKenzie, E. H., Bahkali, A. H. &amp; Hyde, K. D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95 - 129. https: // doi. org / 10.1007 / s 13225 - 012 - 0198 - 1" type="journal article" year="2012">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD0E56935FCA9FCB9" box="[402,456,770,796]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2012
</bibRefCitation>
). During the revision of
<taxonomicName id="4C2A51B3535BFF9FD2496934FEBAFCB9" box="[830,987,770,796]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="genus">
<emphasis id="B95EF622535BFF9FD2496934FEBAFCB9" box="[830,987,770,796]" italics="true" pageId="12" pageNumber="103">Pestalotiopsis</emphasis>
</taxonomicName>
,
<taxonomicName id="4C2A51B3535BFF9FD2996935F9EEFCB9" box="[1006,1167,770,796]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophyta">
<emphasis id="B95EF622535BFF9FD2996935F9EEFCB9" box="[1006,1167,770,796]" italics="true" pageId="12" pageNumber="103">P. saprophyta</emphasis>
</taxonomicName>
was synonymised to
<taxonomicName id="4C2A51B3535BFF9FD4E56935FC7BFCE5" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD4E56935FC7BFCE5" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
by
<bibRefCitation id="EFBB57C1535BFF9FD03A6910FF80FCE5" author="Maharachchikumbura, S. S. N. &amp; Hyde, K. D. &amp; Groenewald, J. Z. &amp; Xu, J. &amp; Crous, P. W." box="[333,737,806,833]" pageId="12" pageNumber="103" pagination="121 - 186" refId="ref15008" refString="Maharachchikumbura, S. S. N., Hyde, K. D., Groenewald, J. Z., Xu, J. and Crous, P. W. (2014) Pestalotiopsis revisited. Studies in Mycology 79: 121 - 186. https: // doi. org / 10.1016 / j. simyco. 2014.09.005" type="journal article" year="2014">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD3396911FFE5FCE5" box="[590,644,806,832]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. (2014)
</bibRefCitation>
based on the multi-gene phylogeny of ITS,
<emphasis id="B95EF622535BFF9FD5866910F845FCE5" box="[1265,1316,806,832]" italics="true" pageId="12" pageNumber="103">tub2</emphasis>
and
<emphasis id="B95EF622535BFF9FD4156910F8C5FCE5" box="[1378,1444,806,832]" italics="true" pageId="12" pageNumber="103">tef1-α</emphasis>
. Our strains (
<collectionCode id="ED3BB2F5535BFF9FD061697CFCEAFCC1" box="[278,395,842,868]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0259 and
<collectionCode id="ED3BB2F5535BFF9FD35C697CFFFFFCC1" box="[555,670,842,868]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0260) share similar conidial characteristics with
<taxonomicName id="4C2A51B3535BFF9FD580697DF8CAFCC1" box="[1271,1451,842,868]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD580697DF8CAFCC1" box="[1271,1451,842,868]" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
(
<collectionCode id="ED3BB2F5535BFF9FD1F86958FC65FC2D" box="[143,260,878,904]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
12-0282) by having same conidial length to width ratio (4.4), with three median cells that are dark brown to olivaceous, and 24, long apical appendages (13.8 vs 13.6 μm). However, conidial sizes of our isolates (19.450.9 × 4.612.6 μm) show significant differences from those of
<taxonomicName id="4C2A51B3535BFF9FD2DB6981F901FC75" box="[940,1120,950,976]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD2DB6981F901FC75" box="[940,1120,950,976]" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
(
<collectionCode id="ED3BB2F5535BFF9FD5076980F984FC75" box="[1136,1253,950,976]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
12-0282) (2230 × 56 μm) (
<bibRefCitation id="EFBB57C1535BFF9FD06569ECFFF0FC51" author="Maharachchikumbura, S. S. N. &amp; Guo, L. D. &amp; Cai, L. &amp; Chukeatirote, E. &amp; Wu, W. P. &amp; Sun, X. &amp; Crous, P. W. &amp; Bhat, D. J. &amp; McKenzie, E. H. &amp; Bahkali, A. H. &amp; Hyde, K. D." box="[274,657,986,1013]" pageId="12" pageNumber="103" pagination="95 - 129" refId="ref14892" refString="Maharachchikumbura, S. S. N., Guo, L. D., Cai, L., Chukeatirote, E., Wu, W. P., Sun, X., Crous, P. W., Bhat, D. J., McKenzie, E. H., Bahkali, A. H. &amp; Hyde, K. D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95 - 129. https: // doi. org / 10.1007 / s 13225 - 012 - 0198 - 1" type="journal article" year="2012">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD36569EDFF24FC51" box="[530,581,986,1012]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. 2012
</bibRefCitation>
), along with median cell length (2.57.6 μm
<emphasis id="B95EF622535BFF9FD5E169EDF9CCFC51" box="[1174,1197,987,1012]" italics="true" pageId="12" pageNumber="103">vs</emphasis>
46 μm) and conidial colour (concoloured olivaceous vs versicoloured dark brown to olivaceous). According to the multi-locus phylogeny,
<taxonomicName id="4C2A51B3535BFF9FD1FF6E15FC5CFB99" box="[136,317,1058,1084]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD1FF6E15FC5CFB99" box="[136,317,1058,1084]" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
(CBS 115452) clustered with
<collectionCode id="ED3BB2F5535BFF9FD3D76E14FE72FB99" box="[672,787,1058,1084]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0259 with 98%
<collectionCode id="ED3BB2F5535BFF9FD28B6E14F92AFB99" box="[1020,1099,1058,1084]" pageId="12" pageNumber="103">MLBS</collectionCode>
, 56%
<collectionCode id="ED3BB2F5535BFF9FD5E16E14F982FB99" box="[1174,1251,1058,1084]" pageId="12" pageNumber="103">MPBS</collectionCode>
, 0.99
<collectionCode id="ED3BB2F5535BFF9FD45B6E15F814FB98" box="[1324,1397,1059,1085]" pageId="12" pageNumber="103">BYPP</collectionCode>
, and
<collectionCode id="ED3BB2F5535BFF9FD1FF6E70FD9AFBC5" box="[136,251,1094,1120]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0259 with 91%
<collectionCode id="ED3BB2F5535BFF9FD0956E70FF50FBC5" box="[482,561,1094,1120]" pageId="12" pageNumber="103">MLBS</collectionCode>
, 45%
<collectionCode id="ED3BB2F5535BFF9FD30C6E70FFA7FBC5" box="[635,710,1094,1120]" pageId="12" pageNumber="103">MPBS</collectionCode>
and 0.51
<collectionCode id="ED3BB2F5535BFF9FD24D6E71FEE5FBC4" box="[826,900,1095,1121]" pageId="12" pageNumber="103">BYPP</collectionCode>
. Base pair comparison between
<taxonomicName id="4C2A51B3535BFF9FD5816E71F8CAFBC5" box="[1270,1451,1094,1120]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD5816E71F8CAFBC5" box="[1270,1451,1094,1120]" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
(CBS 115452) and our isolate
<collectionCode id="ED3BB2F5535BFF9FD0946E5CFF37FB21" box="[483,598,1130,1156]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0259 demonstrated insignificant nucleotide differences only in the
<emphasis id="B95EF622535BFF9FD40D6E5CF8CDFB21" box="[1402,1452,1130,1156]" italics="true" pageId="12" pageNumber="103">tub2</emphasis>
(0.13%) region, while the isolate
<collectionCode id="ED3BB2F5535BFF9FD3776EB8FF12FB0D" box="[512,627,1166,1192]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="12" pageNumber="103">MFLUCC</collectionCode>
23-0260 showed slight nucleotide differences in
<emphasis id="B95EF622535BFF9FD5E96EB8F9B1FB0D" box="[1182,1232,1166,1192]" italics="true" pageId="12" pageNumber="103">tub2</emphasis>
(0.27%) and
<emphasis id="B95EF622535BFF9FD41E6EB8F8CAFB0D" box="[1385,1451,1166,1192]" italics="true" pageId="12" pageNumber="103">tef1-α</emphasis>
(0.67%) regions. Thus, we identify our strains as
<taxonomicName id="4C2A51B3535BFF9FD3D86E85FE00FB69" box="[687,865,1202,1228]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="103" phylum="Ascomycota" rank="species" species="saprophytica">
<emphasis id="B95EF622535BFF9FD3D86E85FE00FB69" box="[687,865,1202,1228]" italics="true" pageId="12" pageNumber="103">N. saprophytica</emphasis>
</taxonomicName>
, a new record on
<taxonomicName id="4C2A51B3535BFF9FD55F6E84F8EEFB69" box="[1064,1423,1202,1229]" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" pageId="12" pageNumber="103" phylum="Tracheophyta" rank="variety" species="sinensis" variety="assamica">
<emphasis id="B95EF622535BFF9FD55F6E84F98EFB69" box="[1064,1263,1202,1228]" italics="true" pageId="12" pageNumber="103">Camellia sinensis</emphasis>
var.
<emphasis id="B95EF622535BFF9FD4506E85F8EEFB69" box="[1319,1423,1203,1228]" italics="true" pageId="12" pageNumber="103">assamica</emphasis>
</taxonomicName>
in
<collectingCountry id="F33D6AA0535BFF9FD1FF6EE0FD8FFB55" box="[136,238,1238,1264]" name="Thailand" pageId="12" pageNumber="103">Thailand</collectingCountry>
, following the species recognition guidelines provided by
<bibRefCitation id="EFBB57C1535BFF9FD2F56EE0F91FFB55" author="Chethana, K. T. &amp; Manawasinghe, I. S. &amp; Hurdeal, V. G. &amp; Bhunjun, C. S. &amp; Appadoo, M. A. &amp; Gentekaki, E. &amp; Raspe, O. &amp; Promputtha, I. &amp; Hyde, K. D." box="[898,1150,1238,1265]" pageId="12" pageNumber="103" pagination="1 - 25" refId="ref12161" refString="Chethana, K. T., Manawasinghe, I. S., Hurdeal, V. G., Bhunjun, C. S., Appadoo, M. A., Gentekaki, E., Raspe, O., Promputtha, I. &amp; Hyde, K. D. (2021) What are fungal species and how to delineate them?. Fungal Diversity 109: 1 - 25. https: // doi. org / 10.1007 / s 13225 - 021 - 00483 - 9" type="journal article" year="2021">
Chethana
<emphasis id="B95EF622535BFF9FD2846EE1F944FB55" box="[1011,1061,1238,1264]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. (2021)
</bibRefCitation>
and
<bibRefCitation id="EFBB57C1535BFF9FD5C16EE0FC74FAB1" author="Maharachchikumbura, S. S. N. &amp; Yanpeng, C. &amp; Ariyawansa, H. A. &amp; Hyde, K. D. &amp; Haelewaters, D. &amp; Perera, R. H. &amp; Samarakoon, M. C. &amp; Wanasinghe, D. N. &amp; Bustamante, D. E. &amp; Liu, J. K. &amp; Lawrence, D. P." pageId="12" pageNumber="103" pagination="155 - 179" refId="ref15215" refString="Maharachchikumbura, S. S. N., Yanpeng, C., Ariyawansa, H. A., Hyde, K. D., Haelewaters, D., Perera, R. H., Samarakoon, M. C., Wanasinghe, D. N., Bustamante, D. E., Liu, J. K. &amp; Lawrence, D. P. (2021) Integrative approaches for species delimitation in Ascomycota. Fungal Diversity 109: 155 - 179. https: // doi. org / 10.1007 / s 13225 - 021 - 00486 - 6" type="journal article" year="2021">
Maharachchikumbura
<emphasis id="B95EF622535BFF9FD1FF6ECDFDD8FAB1" box="[136,185,1274,1300]" italics="true" pageId="12" pageNumber="103">et al</emphasis>
. (2021)
</bibRefCitation>
.
</paragraph>
</subSubSection>
</treatment>
</document>