Add updates up until 2024-06-14 13:11:53
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<mods:namePart id="90551BD8E5A43F41F0483B6410958EF9">Ramessur, Anusha Devi</mods:namePart>
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<mods:namePart id="05EDCBAC4A0BABF8264EBF7D4D014D50">Bothwell, John H.</mods:namePart>
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<mods:namePart id="9D2D562060405ECB460B13859BD42B29">Maggs, Christine A.</mods:namePart>
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<mods:namePart id="72BA05DBA6D65379C1337BC5E19F0153">Gan, Sook Yee</mods:namePart>
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<mods:namePart id="D19FA34A0AE6613C25AB9556EA5AC1CA">Phang, Siew Moi</mods:namePart>
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<mods:namePart id="A7EF2E20CA0B57AE6FD67DB0DF61B3B8">Phang, Siew Moi</mods:namePart>
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<mods:date id="F21E630DEB1351326903939EA8186726">2018</mods:date>
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<mods:title id="EAC90E709C126FC31205DA33AAC573AE">Warsaw, Poland</mods:title>
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<mods:date id="AC3D35FC4F0C5D31D1FF211857B3CC5C">2018</mods:date>
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<mods:number id="D9A4C764A816CE9E98E10059A29E8CDC">2018-09-12</mods:number>
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<mods:number id="506290FC03E64194EBC96001F8D5CA25">2018-09-12</mods:number>
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<treatment id="038F146EFFCEA12F507596C46B70DF8F" LSID="urn:lsid:plazi:treatment:038F146EFFCEA12F507596C46B70DF8F" httpUri="http://treatment.plazi.org/id/038F146EFFCEA12F507596C46B70DF8F" lastPageId="5" lastPageNumber="503" pageId="3" pageNumber="502">
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<treatment id="038F146EFFCEA12F507596C46B70DF8F" ID-DOI="http://doi.org/10.5281/zenodo.11494493" ID-GBIF-Taxon="232143092" ID-Zenodo-Dep="11494493" LSID="urn:lsid:plazi:treatment:038F146EFFCEA12F507596C46B70DF8F" httpUri="http://treatment.plazi.org/id/038F146EFFCEA12F507596C46B70DF8F" lastPageId="5" lastPageNumber="503" pageId="3" pageNumber="502">
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<subSubSection id="C33CF6F3FFCEA129507596C46D89DF89" pageId="3" pageNumber="502" type="nomenclature">
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<paragraph id="8B99A578FFCEA129507596C46D89DF89" blockId="3.[824,1412,1673,1744]" pageId="3" pageNumber="502">
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@ -128,7 +130,7 @@ confirmed that this cefotaxime-supplemented wash protocol ensured that residual
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<emphasis id="B952796AFFC9A12E51CB93136999DADC" bold="true" italics="true" pageId="4" pageNumber="503">Agrobacterium</emphasis>
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</taxonomicName>
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and other culturable bacteria were eliminated on the thalli within 6 days (
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<tableCitation id="C6A490C3FFC9A12E522B93E86897DAFB" box="[358,436,933,957]" captionStart="Table 1" captionStartId="4.[136,186,1325,1345]" captionTargetPageId="4" captionText="Table 1: Confirmation of the suitability of 500 and 800 mg l−1 cefotaxime in eliminating bacteria." pageId="4" pageNumber="503">Table 1</tableCitation>
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<tableCitation id="C6A490C3FFC9A12E522B93E86897DAFB" box="[358,436,933,957]" captionStart="Table 1" captionStartId="4.[136,186,1325,1345]" captionTargetPageId="4" captionText="Table 1: Confirmation of the suitability of 500 and 800 mg l−1 cefotaxime in eliminating bacteria." httpUri="http://table.plazi.org/id/DF59F5F0FFC9A12E53C5956068EBDC18" pageId="4" pageNumber="503" tableUuid="DF59F5F0FFC9A12E53C5956068EBDC18">Table 1</tableCitation>
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).
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</paragraph>
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<paragraph id="8B99A578FFC9A12E53C594456B49DD08" blockId="4.[136,655,1032,1102]" pageId="4" pageNumber="503">
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@ -184,7 +186,7 @@ As shown in
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<figureCitation id="131DB9FDFFC9A12E50EA955C6D21DC6F" box="[935,1026,1297,1321]" captionStart="Figure 4" captionStartId="5.[167,224,975,995]" captionTargetBox="[167,792,654,944]" captionTargetId="figure-449@5.[306,788,676,873]" captionTargetPageId="5" captionText="Figure 4: Effect of cultivation medium, wounding method and acetosyringone on Agrobacterium transformation efficiency. The transformation efficiencies of Chondrus thalli segments co-cultivated with pCAMBIA 1301-transformed Agrobacterium in seawater and Induction Medium (IM) were compared. Using seawater as co-cultivation medium, the wound-related treatments, namely biolistics wounding, wounding using pin-pricks and no wounding, were compared. The effect of acetosyringone (100 µM) was also studied. Data are expressed as means ± SE (n = 4). The Mann-Whitney non-parametric test was conducted to compare between two groups." figureDoi="http://doi.org/10.5281/zenodo.11360097" httpUri="https://zenodo.org/record/11360097/files/figure.png" pageId="4" pageNumber="503">Figure 4</figureCitation>
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, among the pin-pricked thalli, a significant increase in transformation efficiency (higher percentage of thallus segments stained blue) was observed when seawater was used in preference to Induction Medium (Mann-Whitney test, p = 0.029) in the presence of acetosyringone. However, no transformation was detected in the unwounded thallus segments or those wounded by bombardment when co-cultivation was conducted using seawater. In addition, without the presence of acetosyringone, transformation occurred at a significantly lower efficiency of less than 10% (Mann-Whitney, p = 0.004).
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</paragraph>
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<caption id="DF59F5F0FFC9A12E53C5956068EBDC18" ID-Table-UUID="DF59F5F0FFC9A12E53C5956068EBDC18" httpUri="http://table.plazi.org/id/DF59F5F0FFC9A12E53C5956068EBDC18" pageId="4" pageNumber="503" startId="4.[136,186,1325,1345]" targetBox="[136,764,1417,1690]" targetIsTable="true" targetPageId="4" targetType="table">
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<paragraph id="8B99A578FFC9A12E53C5956068EBDC18" blockId="4.[136,694,1324,1374]" pageId="4" pageNumber="503">
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<emphasis id="B952796AFFC9A12E53C595606B91DC7F" bold="true" box="[136,690,1324,1345]" pageId="4" pageNumber="503">
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Table 1: Confirmation of the suitability of 500 and 800 mg
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