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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.44.24998" ID-GBIF-Dataset="26bbead9-f466-4886-bace-a5ed686e5223" ID-PMC="PMC6302069" ID-Pensoft-Pub="1314-4049-44-1" ID-PubMed="30588166" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-44-1" ModsDocOrigin="MycoKeys 44" ModsDocTitle="Three new species of EntolomasubgenusPouzarella from China based on morphological and molecular data" checkinTime="1555333451517" checkinUser="pensoft" docAuthor="He, Xiao-Lan, Horak, Egon, Wang, Di, Peng, Wei-Hong &amp; Gan, Bing-Cheng" docDate="2018" docId="5E4D4E27A88801D84BC24FC49763503D" docLanguage="en" docName="MycoKeys 44: 1-18" docOrigin="MycoKeys 44" docSource="http://dx.doi.org/10.3897/mycokeys.44.24998" docTitle="Entoloma griseocarpum Xiao L. He &amp; E. Horak, sp. nov." docType="treatment" docVersion="4" lastPageNumber="1" masterDocId="5E6D7C57080B056DFFCCD231FFF6FFE9" masterDocTitle="Three new species of EntolomasubgenusPouzarella from China based on morphological and molecular data" masterLastPageNumber="18" masterPageNumber="1" pageNumber="1" updateTime="1668136119543" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>Three new species of EntolomasubgenusPouzarella from China based on morphological and molecular data</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>He, Xiao-Lan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Horak, Egon</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Di</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Peng, Wei-Hong</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Gan, Bing-Cheng</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:title>MycoKeys</mods:title>
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<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>44</mods:number>
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<mods:start>1</mods:start>
<mods:end>18</mods:end>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.44.24998</mods:url>
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<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.44.24998</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-44-1</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="156202143" LSID="urn:lsid:plazi:treatment:5E4D4E27A88801D84BC24FC49763503D" httpUri="http://treatment.plazi.org/id/5E4D4E27A88801D84BC24FC49763503D" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
<paragraph pageId="0" pageNumber="1">
<taxonomicName LSID="828701" authority="Xiao L. He &amp; E. Horak" class="Agaricomycetes" family="Entolomataceae" genus="Entoloma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Entoloma griseocarpum" order="Agaricales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="species" species="griseocarpum">Entoloma griseocarpum Xiao L. He &amp; E. Horak</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
Figs 1c, d, 3
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="diagnosis">
<paragraph pageId="0" pageNumber="1">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="1">
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
is characterised by the greyish-brown pileus, large basidiospores (12.5-15.5
<normalizedToken originalValue="×">x</normalizedToken>
7.5-9
<normalizedToken originalValue="µm">µm</normalizedToken>
) and broadly clavate, ovoid to lageniform cheilocystidia.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="materials_examined">
<paragraph pageId="0" pageNumber="1">Type.</paragraph>
<paragraph pageId="0" pageNumber="1">
CHINA. TIBET: Linzhi, Lulang,
<geoCoordinate direction="north" orientation="latitude" precision="925" value="30.566668">29°94'N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="925" value="95.316666">94°79'E</geoCoordinate>
, elevation ca. 3800 m, 18 September 2014, He X.L. (SAAS 1328, holotype).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="etymology">
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="1">griseocarpum, refers to the greyish-brown coloured basidiomes.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
<paragraph pageId="0" pageNumber="1">
Pileus 5-20 mm broad, hemispherical, convex, bluntly conic to broadly campanulate, dry, not hygrophanous, greyish-brown to brown (4D3-4E3), densely covered by suberect hispid or minutely squamulose overall, denser in centre; fibrils dark grey, pale grey brownish or concolorous with pileal surface (4D2-4D3), striate from entire margin to near centre. Lamellae sinuate with short decurrent tooth, ventricose, distant, moderately thick, up to 3 mm broad, with two tiers of lamellulae, dark grey to brownish-grey, with entire and concolorous edges. Stipe 20-50
<normalizedToken originalValue="×">x</normalizedToken>
0.7-1.5 mm, cylindrical, equal, dry, concolorous with pileus, densely covered with pale yellow brownish flocculose hairs, hollow, with a dirty yellowish to pale yellow brownish strigose base. Context thin, concolorous with pileus. Odour and taste not distinctive.
</paragraph>
<paragraph pageId="0" pageNumber="1">
Basidiospores 12.5-15.5 (-17)
<normalizedToken originalValue="×">x</normalizedToken>
(6.5-) 7.5-9 (-9.5)
<normalizedToken originalValue="µm">µm</normalizedToken>
(x = 13.8
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="×">x</normalizedToken>
8.3
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="µm">µm</normalizedToken>
), Q = 1.60-1.94 (Q = 1.71
<normalizedToken originalValue="±">+/-</normalizedToken>
0.02), heterodiametric, strongly angled in profile and face view with 6-10 facets, appearing nodulose, pale yellow brownish, thick-walled. Basidia 35-55
<normalizedToken originalValue="×">x</normalizedToken>
11-13 (-15)
<normalizedToken originalValue="µm">µm</normalizedToken>
, subclavate to clavate, 4-spored. Aborted basidia scattered in the hymenium, filled with dark brown amorphous cytoplasmic pigment. Lamellar trama dark brown, composed of parallel, cylindrical, heavily encrusted and thin-walled elements. Lamellar edges sterile. Cheilocystidia 23-50
<normalizedToken originalValue="×">x</normalizedToken>
(10-) 12 20
<normalizedToken originalValue="µm">µm</normalizedToken>
, broadly clavate, ovoid to lageniform; with brownish, intracellular pigment, slightly thick-walled. Pileipellis a trichoderm composed of yellow brown, suberect and multiseptate hyphae, walls heavily encrusted with brown pigment; terminal cells 35-105
<normalizedToken originalValue="×">x</normalizedToken>
8-27
<normalizedToken originalValue="µm">µm</normalizedToken>
, cylindrical, subclavate or bullet-shaped, thin to moderately thick-walled; subpellis composed of cylindrical encrusted hyphae, up to 25
<normalizedToken originalValue="µm">µm</normalizedToken>
diam. Stipitipellis composed of yellow-brown encrusted hyphae; terminal cells 40-80
<normalizedToken originalValue="×">x</normalizedToken>
4-10
<normalizedToken originalValue="µm">µm</normalizedToken>
, slender cylindrical with obtuse apex, thin-walled, sparsely encrusted with pale yellowish-brown pigment. Oleiferous hyphae absent. Clamp connections absent.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="habitat">
<paragraph pageId="0" pageNumber="1">Habitat.</paragraph>
<paragraph pageId="0" pageNumber="1">
Scattered on soil amongst decaying litter in mixed conifer-broadleaf forest dominated by
<taxonomicName class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Betulaceae" genus="Betula" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Betula" order="Fagales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Betula</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Ericaceae" genus="Rhododendron" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Rhododendron" order="Ericales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Rhododendron</taxonomicName>
and
<taxonomicName class="Pinopsida" family="Pinaceae" genus="Abies" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Abies" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Abies</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="additional collections examined">
<paragraph pageId="0" pageNumber="1">Additional collections examined.</paragraph>
<paragraph pageId="0" pageNumber="1">
CHINA. TIBET: Linzhi, Lulang,
<geoCoordinate direction="north" orientation="latitude" precision="925" value="30.566668">29°94'N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="925" value="95.316666">94°79'E</geoCoordinate>
, elevation ca. 3800 m, 18 September 2014, He X.L. (SAAS 1230, SAAS 1657, SAAS 1751, SAAS 1871). SICHUAN PROV.: Jiuzhaigou,
<geoCoordinate direction="north" orientation="latitude" precision="925" value="33.466667">33°28'N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="925" value="103.98333">103°59'E</geoCoordinate>
, elevation ca. 3000 m, 20 July 2013, He X.L. (SAAS 951).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="comments">
<paragraph pageId="0" pageNumber="1">Comments.</paragraph>
<paragraph pageId="0" pageNumber="1">
The greyish-brown pileus covered by suberect hispid or minutely squamulose, the brown external encrustations on pileipellis and stipitipellis and the cylindrical terminal cells of pileipellis and stipitipellis indicate
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
belongs to the sect.
<taxonomicName class="Agaricomycetes" family="Entolomataceae" genus="Dysthales" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Dysthales" order="Agaricales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="section" section="Dysthales">Dysthales</taxonomicName>
. It is very similar to
<taxonomicName lsidName="E. albostrigosum" pageId="0" pageNumber="1" rank="species" species="albostrigosum">E. albostrigosum</taxonomicName>
(Largent &amp; Abell-Davis) Blanco-Dios and
<taxonomicName lsidName="E. lasium" pageId="0" pageNumber="1" rank="species" species="lasium">E. lasium</taxonomicName>
(Berk. &amp; Broome) Noordel. &amp; Co-David (
<bibRefCitation author="Largent, DL" journalOrPublisher="The Korean Journal of Mycology" pageId="0" pageNumber="1" url="https://doi.org/10.5248/117.435" year="2011">Largent et al. 2011</bibRefCitation>
). However,
<taxonomicName lsidName="E. albostrigosum" pageId="0" pageNumber="1" rank="species" species="albostrigosum">E. albostrigosum</taxonomicName>
is distinguished by the white strigose base and
<taxonomicName lsidName="E. lasium" pageId="0" pageNumber="1" rank="species" species="lasium">E. lasium</taxonomicName>
differs by the smaller basidiospores (8.9-14.5
<normalizedToken originalValue="×">x</normalizedToken>
5.1-8.7
<normalizedToken originalValue="μm">μm</normalizedToken>
,
<bibRefCitation author="Largent, DL" journalOrPublisher="The Korean Journal of Mycology" pageId="0" pageNumber="1" url="https://doi.org/10.5248/117.435" year="2011">Largent et al. 2011</bibRefCitation>
). In addition, the two species are distant from
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
following phylogenetic analysis.
<taxonomicName lsidName="E. puertoricense" pageId="0" pageNumber="1" rank="species" species="puertoricense">E. puertoricense</taxonomicName>
Blanco-Dios (as
<taxonomicName lsidName="P. caribaea" pageId="0" pageNumber="1" rank="species" species="caribaea">P. caribaea</taxonomicName>
T.J. Baroni &amp; B. Ortiz in
<bibRefCitation author="Baroni, TJ" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="269 - 279" title="New species of Oudemansiella and Pouzarella (Basidiomycetes: Agaricales) from Puerto Rico." volume="82" year="2002">Baroni and Ortiz 2002</bibRefCitation>
) resembles
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
by the brownish-grey coloured basidiomes but is separated by its broader basidiospores (12.5-16.5
<normalizedToken originalValue="×">x</normalizedToken>
8.3-11.3
<normalizedToken originalValue="μm">μm</normalizedToken>
, Q = 1.26-1.65,
<bibRefCitation author="Baroni, TJ" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="269 - 279" title="New species of Oudemansiella and Pouzarella (Basidiomycetes: Agaricales) from Puerto Rico." volume="82" year="2002">Baroni and Ortiz 2002</bibRefCitation>
). Moreover,
<taxonomicName lsidName="E. puertoricense" pageId="0" pageNumber="1" rank="species" species="puertoricense">E. puertoricense</taxonomicName>
was discovered in a tropical habitat in Puerto Rico (
<bibRefCitation author="Baroni, TJ" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="269 - 279" title="New species of Oudemansiella and Pouzarella (Basidiomycetes: Agaricales) from Puerto Rico." volume="82" year="2002">Baroni and Ortiz 2002</bibRefCitation>
). The similar
<taxonomicName lsidName="E. japonicum" pageId="0" pageNumber="1" rank="species" species="japonicum">E. japonicum</taxonomicName>
(Hongo) Hongo, described from Japan, is also reminiscent of
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
in the brownish pileus but is distinguished by the much larger basidiospores (15-18.5
<normalizedToken originalValue="×">x</normalizedToken>
9-10.5
<normalizedToken originalValue="μm">μm</normalizedToken>
,
<bibRefCitation author="Hongo, T" journalOrPublisher="Journal of Japanese Botany" pageId="0" pageNumber="1" pagination="239 - 246" title="Notes on Japanese larger fungi." volume="34" year="1959">Hongo 1959</bibRefCitation>
). The well-known
<taxonomicName lsidName="E. dysthales" pageId="0" pageNumber="1" rank="species" species="dysthales">E. dysthales</taxonomicName>
(Peck) Sacc. also differs by the larger basidiospores (14-20
<normalizedToken originalValue="×">x</normalizedToken>
7.5-10
<normalizedToken originalValue="µm">µm</normalizedToken>
,
<bibRefCitation author="Mazzer, SJ" journalOrPublisher="Bibliotheca Mycologica" pageId="0" pageNumber="1" pagination="1 - 134" title="A monographic study of the genus Pouzarella." volume="46" year="1976">Mazzer 1976</bibRefCitation>
).
<taxonomicName lsidName="E. fulvolanatum" pageId="0" pageNumber="1" rank="species" species="fulvolanatum">E. fulvolanatum</taxonomicName>
(Berk. &amp; Broome) Blanco-Dios from Sri Lanka is not only separated by its type locality but also by the narrower basidiospores measuring 12-16
<normalizedToken originalValue="×">x</normalizedToken>
7-8
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<bibRefCitation author="Mazzer, SJ" journalOrPublisher="Bibliotheca Mycologica" pageId="0" pageNumber="1" pagination="1 - 134" title="A monographic study of the genus Pouzarella." volume="46" year="1976">Mazzer 1976</bibRefCitation>
). Two species in subgen.
<taxonomicName class="Agaricomycetes" family="Entolomataceae" genus="Dysthales" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Dysthales (Pouzarella)" order="Agaricales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="subGenus" subGenus="Pouzarella">Pouzarella</taxonomicName>
, recently described from geographically neighbouring India, viz.
<taxonomicName lsidName="E. peechiense" pageId="0" pageNumber="1" rank="species" species="peechiense">E. peechiense</taxonomicName>
K. N. A. Raj &amp; Manim. and
<taxonomicName lsidName="E. silvanum" pageId="0" pageNumber="1" rank="species" species="silvanum">E. silvanum</taxonomicName>
K. N. A. Raj &amp; Manim., have somewhat similar basidiomes as compared to
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
; however, their ITS and LSU sequences are distinctly different (
<bibRefCitation author="Raj, KNA" journalOrPublisher="Phytotaxa" pageId="0" pageNumber="1" pagination="101 - 112" title="Four new species of EntolomasubgenusPouzarella from India." url="https://doi.org/10.11646/phytotaxa.307.2.1" volume="307" year="2017">Raj and Manimohan 2017</bibRefCitation>
). The third Indian species
<taxonomicName lsidName="E. lomapadum" pageId="0" pageNumber="1" rank="species" species="lomapadum">E. lomapadum</taxonomicName>
Manim., Joseph &amp; Leelav. is readily recognised by the much smaller basidiospores measuring 11-13
<normalizedToken originalValue="×">x</normalizedToken>
6-9
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<bibRefCitation author="Manimohan, P" journalOrPublisher="Mycological Research" pageId="0" pageNumber="1" pagination="1083 - 1097" title="The genus Entoloma in Kerala State, India." url="https://doi.org/10.1016/S0953-7562(09)80777-6" volume="99" year="1995">Manimohan et al. 1995</bibRefCitation>
). There were four other species in subgen.
<taxonomicName class="Agaricomycetes" family="Entolomataceae" genus="Dysthales" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Dysthales (Pouzarella)" order="Agaricales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="subGenus" subGenus="Pouzarella">Pouzarella</taxonomicName>
which showed some similarities to
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
.
<taxonomicName lsidName="E. fibrillosipes" pageId="0" pageNumber="1" rank="species" species="fibrillosipes">E. fibrillosipes</taxonomicName>
(Murrill) Noordel. &amp; Co-David is distinguished by the much larger basidiospores (17-22
<normalizedToken originalValue="×">x</normalizedToken>
7.5-10
<normalizedToken originalValue="μm">μm</normalizedToken>
,
<bibRefCitation author="Mazzer, SJ" journalOrPublisher="Bibliotheca Mycologica" pageId="0" pageNumber="1" pagination="1 - 134" title="A monographic study of the genus Pouzarella." volume="46" year="1976">Mazzer 1976</bibRefCitation>
).
<taxonomicName lsidName="E. subdeceptivum" pageId="0" pageNumber="1" rank="species" species="subdeceptivum">E. subdeceptivum</taxonomicName>
Courtec. and
<taxonomicName lsidName="E. rotula" pageId="0" pageNumber="1" rank="species" species="rotula">E. rotula</taxonomicName>
(Romagn.) Noordel. &amp; Co-David are lignicolous (
<bibRefCitation author="Mazzer, SJ" journalOrPublisher="Bibliotheca Mycologica" pageId="0" pageNumber="1" pagination="1 - 134" title="A monographic study of the genus Pouzarella." volume="46" year="1976">Mazzer 1976</bibRefCitation>
).
<taxonomicName lsidName="E. homomorphum" pageId="0" pageNumber="1" rank="species" species="homomorphum">E. homomorphum</taxonomicName>
(Romagn.) Singer differs by the larger basidiospores (15-19
<normalizedToken originalValue="×">x</normalizedToken>
9-11.5
<normalizedToken originalValue="μm">μm</normalizedToken>
,
<bibRefCitation author="Mazzer, SJ" journalOrPublisher="Bibliotheca Mycologica" pageId="0" pageNumber="1" pagination="1 - 134" title="A monographic study of the genus Pouzarella." volume="46" year="1976">Mazzer 1976</bibRefCitation>
).
</paragraph>
<caption pageId="0" pageNumber="1">
<paragraph pageId="0" pageNumber="1">
Figure 3. Microscopic structures of
<taxonomicName lsidName="E. griseocarpum" pageId="0" pageNumber="1" rank="species" species="griseocarpum">E. griseocarpum</taxonomicName>
(drawn from the holotype). a Basidiospores b Cheilocystidia c Pileipellis.
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>