From 41ea1eb3cb93b49a31f8292a92e4b3950fd43f64 Mon Sep 17 00:00:00 2001 From: ggserver Date: Thu, 20 Feb 2025 00:35:02 +0000 Subject: [PATCH] Add updates up until 2025-02-20 00:28:55 --- .../5D/03AC5D376843FFE8FE7B5329BBCBFCA2.xml | 816 +++++++ .../5D/03AC5D376848FFEEFE7C574DBBBEFCC7.xml | 1898 ----------------- .../5D/03AC5D376848FFF5FE7C574DBBD2FCA1.xml | 565 +++++ .../5D/03AC5D37684CFFFFFD9856A6BBE7FAED.xml | 100 +- .../5D/03AC5D37685CFFEEFE045329BBBEFCC7.xml | 602 ++++++ 5 files changed, 2035 insertions(+), 1946 deletions(-) create mode 100644 data/03/AC/5D/03AC5D376843FFE8FE7B5329BBCBFCA2.xml delete mode 100644 data/03/AC/5D/03AC5D376848FFEEFE7C574DBBBEFCC7.xml create mode 100644 data/03/AC/5D/03AC5D376848FFF5FE7C574DBBD2FCA1.xml create mode 100644 data/03/AC/5D/03AC5D37685CFFEEFE045329BBBEFCC7.xml diff --git a/data/03/AC/5D/03AC5D376843FFE8FE7B5329BBCBFCA2.xml b/data/03/AC/5D/03AC5D376843FFE8FE7B5329BBCBFCA2.xml new file mode 100644 index 00000000000..3aea18502dd --- /dev/null +++ b/data/03/AC/5D/03AC5D376843FFE8FE7B5329BBCBFCA2.xml @@ -0,0 +1,816 @@ + + + +Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) + + + +Author + +Holovachov, Oleksandr +89D30ED8-CFD2-42EF-B962-30A13F97D203 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +oleksandr.holovachov@nrm.se + + + +Author + +Boström, Sven +528300CC-D0F0-4097-9631-6C5F75922799 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +sven.bostrom@nrm.se + +text + + +European Journal of Taxonomy + + +2018 + +2018-08-29 + + +458 + + +1 +34 + + + +journal article +22251 +10.5852/ejt.2018.458 +4a6349ea-1928-45b5-855f-bd13b697bb49 +3826987 +16C565AB-22A6-496F-AE95-0A876066F958 + + + + + +Neodiplopeltula barentsi +( +Steiner, 1916 +) + +gen. et comb. nov. +Figs 5–7 +, +14–15 +; +Table 3 + + + + +Diagnosis +(based on combined data) + + + +Neodiplopeltula barentsi + +gen. et comb. nov. +is characterised by a +0.94–1.67 mm +long body; refractive plates underlying cephalic cuticle around amphids present, elongated ovoid in shape, not joined, +23.5– 32 µm +long and +12–16 µm +wide; cephalic setae +3.5–7.5 µm +long; amphidial fovea +21–32 µm +long and +4–7 µm +wide; oral opening 1–2 from anterior end, posterior to cephalic setae bases; secretory-excretory + + + +Fig. 5. + +Neodiplopeltula barentsi +( +Steiner, 1916 +) + +gen. et comb. nov. +(SMNH-169268). +A +. Male anterior end, surface view. +B +. Anterior part of female reproductive system. +C +. Female anterior end, surface view. +D +. Female pharyngeal region, optical median section. +E +. Male tail. +F +. Female tail. Scale bar = 20 µm. + + + +pore located opposite to posterior part of pharynx; tail 1.6–3.3 anal body diameters long; vagina with epiptygmata; spicules +45–73 µm +long. + + + + + + +Material examined + + + + + +SWEDEN +: +1 ♀ +, +1 ♂ +, +Skagerrak +, +58°20′06″ N +, +11°09′24″ E +, muddy sediment at a deep of + + +53 m + + +, + +9 Aug. 2011 + +, +O. Holovachov +leg. (SMNH-169264) + +; + +1 ♀ +, +1 ♂ +, +Skagerrak +, +58°22′19″ N +, +11°04′55″ E +, muddy sediment at a deep of + + +55– +70 m + + +, + +9 Aug. 2011 + +, +O. Holovachov +leg. (SMNH-169266); + + +3 ♀♀ +, +1 ♂ +, +Gullmarn Fjord near Östersidan +, +58°15′09″ N +, +11°27′54″ E +, shells, gravel sand and mud, + +14 Aug. 2014 + +, +O. Holovachov +leg. (SMNH-169268); + + +1 ♀ +lectotype +of + + +Diplopeltis +asymmetricus + +, +Öresund + +, west of +Valgrundet +, at a deep of + + +30– +36 m + + +, + + +Zostera + +and + +Echinocardium + + +, + +7 Aug. 1926 + +( +SMNH +Type-9015). + + + + +GREENLAND +: +1 ♀ +, +holotype +of + +Diplopeltis ovalis +, Godhavn + +, mud, +17 Aug. 1926 +, Reisinger and Steinböck leg. ( +NHMD +102171) + +. + + + + + +Description + + + +Adult + + +Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body, except for visually smooth labial region and terminal part of tail (striation can be observed under LM but very fine and shallow and cannot be measured with confidence); longitudinal striation not observed. Somatic sensilla visible along pharyngeal region (cervical sensilla, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates elongated ovoid in shape (plate edges not incised), not connected with each other on ventral and on dorsal sides. Inner labial sensilla indistinct. Outer labial sensilla papilliform, located on anterior surface of lips. Cephalic sensilla setiform, bases of dorsosublateral setae located posterior to oral opening. Cervical sensilla papilliform, arranged in four sublateral rows starting at level with middle of amphid and ending at level with posterior edge of refractive plate, one or two per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch usually 0.5–2.0 µm longer than ventral branch (in two specimens ventral branch 0.5–1.0 µm longer than dorsal branch). Oral opening shifted towards dorsal side of body. Stoma barrel-shaped: cheilostom broad; gymnostom subcylindrical, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia ovoid, entirely embedded into intestinal tissue. Secretoryexcretory system present; secretory-excretory pore along ventral body line opposite to 3/5 +th +of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail conoid with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. + + + +Fig. 6. + +Neodiplopeltula barentsi +( +Steiner, 1916 +) + +gen. et comb. nov. +(SMNH-169268). +A +. ♂, entire specimen. +B +. ♀, entire specimen. +C +. Anterior testis. +D +. Posterior testis. Scale bars: A–B = 100 µm; C–D = 50 µm. + + + + +Table 3 +(continued on next page). Measurements (in µm) of + +Neodiplopeltula barentsi +( +Steiner, 1916 +) + +gen. et comb. nov. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + + +D. ovalis + + + +D. asymmetricus + + + + +D. onustus +sensu Sergeeva (1974) + + + + +data for + +D. cuspidiboja + + + +recent specimens +
♂♀ holotype♀ lectotype♀♂♂5 ♀♀3 ♂♂
Body length152612289701057114711471207 ± 258 (935–1532)1487 ± 285 (1159–1670)
Body diameter (BD)?616539454452 ± 13 (41–74)55 ± 15 (38–65)
Pharynx length205197159169180175176 ± 11 (168–193)182 ± 15 (165–195)
Tail length122956473796784 ± 8 (76–96)98 ± 9 (91–108)
Anal body diameter (ABD)?384022343235 ± 6 (29–43)41 ± 6 (34–46)
a?20.114.926.825.52623.3 ± 4.0 (19.1–29.6)27.7 ± 2.9 (25.1–30.8)
b7.46.26.16.36.476.8 ± 1.1 (5.5–8.0)8.1 ± 1.0 (7.0–8.9)
c12.512.915.214.513.61714.3 ± 2.0 (12.0–16.0)15.2 ± 2.6 (12.7–17.9)
c’?2.51.63.32.32.12.4 ± 0.3 (2.2–2.8)2.4 ± 0.3 (2.1–2.7)
V(%) / T(%)?60.764.262.3??61.5 ± 1.9 (59.6–63.5)48.1 ± 4.7 (43.6–53.1)
Labial region diameter?161812171714.9 ± 0.8 (14.0–16.0)15.0 ± 1.1 (14.0–16.0)
Cephalic setae length???7.57.545.5 ± 1.7 (3.5–7.5)7.4 ± 0.4 (7.0–7.5)
Subdorsal cephalic setae from anterior end?????116.6 ± 0.6 (5.5–7.0)7.6 ± 0.7 (7.0–8.0)
Subventral cephalic setae from anterior end?????96.5 ± 0.6 (5.5–7.0)7.1 ± 0.4 (7.0–7.5)
Cephalic setae length / labial region diam.??????0.4 ± 0.1 (0.2–0.5)0.5
Anterior end of shield from anterior end?10????7.5 ± 0.6 (7.0–8.0)10.0 ± 1.5 (9.0–11.0)
Refractive plate length?25???3225.7 ± 1.5 (23.5–27.0)24.5 ± 0.5 (24.0–25.0)
Refractive plate width?12???12.513.9 ± 1.3 (12.5–16.0)15.2 ± 1.0 (14.5–16.0)
Anterior end of amphid from anterior end?76??65.5 ± 0.7 (5.0–6.0)6.4 ± 1.1 (5.5–7.5)
Dorsal amphid branch length?262629293223.3 ± 2.4 (21.0–25.5)22.5 ± 1.1 (21.5–23.5)
Ventral amphid branch length?2324??3022.6 ± 1.6 (21.0–24.0)21.9 ± 0.4 (21.5–22.0)
Amphid width?676675.1 ± 0.6 (4.0–5.5)5.8 ± 0.4 (5.5–6.0)
Amphid length / width?4.33.74.84.84.64.6 ± 0.9 (3.8–6.2)3.9 ± 0.3 (3.7–4.3)
Anterior edge of stoma from anterior end?11???1.1 ± 0.4 (1.0–1.5)1.4 ± 0.7 (1.0–2.0)
+ + + +Table 3 +(continued). + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + + +D. ovalis + + + +D. asymmetricus + + + + +D. onustus +sensu Sergeeva (1974) + + + + +data for + +D. cuspidiboja + + + +recent specimens +
Stoma width??????5.4 ± 0.6 (5.0–6.0)5.3 ± 0.4 (5.0–5.5)
Nerve ring from anterior end???84967290 ± 5 (84–97)96 ± 10 (86–104)
Nerve ring from anterior end (% pharynx)?????4151.1 ± 1.0 (50.3–52.7)52.7 ± 0.8 (51.7–53.2)
Secretory-excretory pore from ant. end?115???104109 ± 3 (106–112)110 ± 7 (103–115)
Secr.-excr. pore from ant. end (% pharynx)?58.4???6062.2 ± 2.8 (58.0–65.5)60.3 ± 1.6 (59.1–62.1)
Vagina or spicules length????455818.2 ± 1.1 (17.0–19.5)63.8 ± 8.2 (57.0–73.0)
Vagina / BD or spicules / ABD????1.1?0.4 ± 0.1 (0.3–0.5)1.6 ± 0.3 (1.3–1.8)
Rectum or gubernaculum length?????2023.7 ± 5.0 (20.5–32.0)11.0 ± 0.7 (10.0–11.5)
Rectum / ABD or apophysis length??????0.7 ± 0.1 (0.6–0.7)18.6 ± 2.4 (16.0–20.5)
Caudal gland openings from tail terminus?????32.0 ± 0.6 (1.0–2.0)1.9 ± 0.7 (1.0–2.0)
+ + +Female + + +Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube +179–269 µm +long, situated to either right (n = 3) or left (n = 2) of intestine; posterior genital tube +173–286 µm +long, situated to either left (n = 3) or right (n = 2) of intestine. Vulva transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; +pars refringens vaginae +absent. Sacklike spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. + + +Male + + +Reproductive system diorchic, testes opposed; anterior testis +145–314 µm +long, outstretched and posterior testis +117–207 µm +long, reflexed. Spicules paired and symmetrical, strongly curved, with weakly defined elongated manubrium and shaft, cylindrical along most of its length. Gubernaculum plate-like, with pair of strong, closely set caudal apophyses variable in shape ( +Fig. 7 +C–E). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length (often difficult to observe). + + + + + +Remarks + + + +The original description of this species by +Steiner (1916) +is rather short, with a few measurements given. The present population has a shorter pharynx and tail compared to the +type +specimen, but in all other respects both match very well, including the position of the oral opening and the shape of the underlying refractive plates. + + + + \ No newline at end of file diff --git a/data/03/AC/5D/03AC5D376848FFEEFE7C574DBBBEFCC7.xml b/data/03/AC/5D/03AC5D376848FFEEFE7C574DBBBEFCC7.xml deleted file mode 100644 index 51f73246a32..00000000000 --- a/data/03/AC/5D/03AC5D376848FFEEFE7C574DBBBEFCC7.xml +++ /dev/null @@ -1,1898 +0,0 @@ - - - -Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) - - - -Author - -Holovachov, Oleksandr -89D30ED8-CFD2-42EF-B962-30A13F97D203 -Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. -oleksandr.holovachov@nrm.se - - - -Author - -Boström, Sven -528300CC-D0F0-4097-9631-6C5F75922799 -Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. -sven.bostrom@nrm.se - -text - - -European Journal of Taxonomy - - -2018 - -2018-08-29 - - -458 - - -1 -34 - - - -journal article -22251 -10.5852/ejt.2018.458 -4a6349ea-1928-45b5-855f-bd13b697bb49 -3826987 -16C565AB-22A6-496F-AE95-0A876066F958 - - - - - - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen et. comb. nov. - - - - - -Figs 1–4 -; -Table 2 - - - - -Diagnosis -(based on combined data) - - - -Neodiplopeltula incisa - -gen. et comb. nov. -is characterised by a -1.52–2.24 mm -long body; refractive plates underlying cephalic cuticle around amphids present, irregular in shape, not joined, -28–33 µm -long and -21–25 µm -wide; cephalic setae -8–14 µm -long; amphidial fovea -22–28 µm -long and -5.5–7 µm -wide; oral opening -1–3 µm -from anterior end, posterior to cephalic setae bases; secretory-excretory pore located opposite to posterior part of pharynx; tail 2.5–3.9 anal body diameters long; vagina with epiptygmata; spicules -66–79 µm -long. - - - - - - -Material examined - - - - -SWEDEN -: -4 ♀♀ -, 3 ♂♂, Skagerrak, -58°12′37″ N -, -11°18′53″ E -, shells and sand at a deep of -15–22 m -, -10 Oct. 2012 -, O. Holovachov leg. (SMNH-169255); -1 ♀ -, 1 ♂, Skagerrak, -58°20′21″ N -, -11°12′42″ E -, coarse shell sand at a deep of -14–17 m -, -19 Aug. 2014 -, O. Holovachov leg. (SMNH-169258). - - - - -Fig. 1. - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen. et comb. nov. -(SMNH-169255). -A -. Anterior part of female reproductive system. -B -. Male tail. -C -. Female pharyngeal region, optical median section. -D -. Female anterior end, surface view. -E -. Male anterior end, surface view. -F -. Female tail. Scale bar = 20 µm. - - - - - -Description - - - -Adult - - -Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body as seen under SEM (striation can be observed under LM but very fine and shallow and cannot be measured with confidence), except for visually smooth labial region and terminal part of tail; longitudinal striation not observed under the light microscope, but distinct under the scanning electron microscope, covers cuticle over entire body length. Somatic sensilla visible along pharyngeal region (cervical setae, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates irregular in shape (with incised edges), not connected with each other on ventral and dorsal sides. Inner labial sensilla small pore-like, located on anterior surface of lips, discernible under SEM only. Outer labial sensilla papilliform, located on anterior surface of lips, clearly visible under SEM only. Cephalic sensilla setiform, bases of dorsosublateral setae located posterior to oral opening. Cervical sensilla papilliform, arranged in four sublateral rows starting at level with middle of amphid and ending at level with posterior edge of refractive plate, two or three per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch -0.5–3.5 µm -longer than ventral branch. Oral opening shifted towards dorsal side of body. Stoma barrel-shaped: cheilostom broad; gymnostom barrel-shaped, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia ovoid, almost entirely embedded into intestinal tissue. Secretory-excretory system present; secretory-excretory pore located along ventral body line opposite to posterior 3/5 -th -of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail cylindro-conical with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. - - - -Fig. 2. - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen. et comb. nov. -, SEM micrographs. -A–C -, -F–G -. ♂. -D–E -. ♀. -A -. Male anterior end, semi-apical view, dorsal side to the left. -B -. Male labial region, semiapical view, dorsal side down (arrows point at inner labial sensilla). -C -. Male anterior end, right lateral view. -D -. Vulval region. -E -. Tail terminus. -F -. Male cloacal region, lateral view. -G -. Male cloacal region, ventrosubventral view. Scale bars: A, C–D, F–G = 10 µm; B, E = 2 µm. - - - - -Table 2 -(continued on next page). Measurements (in µm) of - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen. et comb. nov. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - -recent specimens -
♀2 ♂♂5 ♀♀4 ♂♂
Body length20202100–22401740 ± 176 (1515–2001)1748 ± 131 (1591–1908)
Body diameter (BD)6253–5848 ± 7 (42–59)45 ± 9 (34–53)
Pharynx length270250–270204 ± 24 (186–245)216 ± 31 (178–254)
Tail length124124–136122 ± 10 (106–135)133 ± 13 (116–147)
Anal body diameter (ABD)455035 ± 3 (32–39)37 ± 1 (37–39.0)
a36.738.6–39.636.9 ± 4.3 (30.0–40.8)39.9 ± 6.1 (32.8–46.5)
b7.58.3–8.48.6 ± 0.7 (7.7–9.6)8.2 ± 0.6 (7.5–8.9)
c16.316.5–1714.4 ± 1.6 (12.4–16.8)13.1 ± 0.4 (12.9–13.7)
c’2.82.53.5 ± 0.3 (2.9–3.8)3.6 ± 0.3 (3.2–3.9)
V(%) / T(%)61?62.7 ± 0.4 (62.1–62.9)45.8 ± 5.1 (40.4–51.6)
Labial region diameter??19.6 ± 1.6 (18.0–22.0)20.9 ± 1.1 (20.0–22.0)
Cephalic setae length??10.6 ± 2.2 (8.0–14.0)11.0 ± 1.0 (10.5–12.0)
Subdorsal cephalic setae from anterior end??7.2 ± 2.0 (5.5–10.5)6.4 ± 0.8 (5.5–7.0)
Subventral cephalic setae from anterior end??7.2 ± 2.0 (5.5–10.5)6.4 ± 0.8 (5.5–7.0)
Cephalic setae length / labial region diam.??0.5 ± 0.1 (0.4–0.8)0.5
Anterior end of plate from anterior end??9.5 ± 1.0 (8.0–10.5)10.0
Refractive plate length??31.4 ± 2.1 (28.0–33.0)32.0 ± 1.1 (31.0–33.0)
Refractive plate width??23.6 ± 2.0 (21.0–25.0)23.2 ± 0.8 (23.0–24.0)
Anterior end of amphid from anterior end??6.2 ± 1.2 (4.0–7.0)6.4 ± 0.8 (5.5–7.0)
Dorsal amphid branch length??27.3 ± 1.8 (25.5–30.0)28.5 ± 1.6 (27.6–30.4)
Ventral amphid branch length??25.4 ± 2.3 (22.0–27.0)27.6 ± 0.7 (26.9–28.3)
Amphid width??5.7 ± 0.3 (5.5–6.0)6.2 ± 0.7 (5.5–6.9)
Amphid length / width??4.8 ± 0.2 (4.6–5.0)4.6 ± 0.3 (4.4–5.0)
- - - -Table 2 -(continued). - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - -recent specimens -
Stoma length??14.4 ± 1.8 (12.5–17.0)16.3 ± 0.8 (16.0–17.0)
Stoma width??5.7 ± 0.3 (5.5–6.0)6.0 ± 0.4 (5.5–6.0)
Nerve ring from anterior end?111–115102 ± 10 (94–114)106 ± 12 (91–120)
Nerve ring from anterior end (% pharynx)?42.6–44.449.7 ± 3.1 (46.5–53.8)49.4 ± 1.7 (47.1–51.3)
Secretory-excretory pore from ant. end??118 ± 13 (108–140)125 ± 19 (99–143)
Secr.-excr. pore from ant. end (% pharynx)??58.2 ± 1.4 (57.0–60.6)57.8 ± 2.2 (55.8–60.4)
Vagina or spicules length?6624.8 ± 3.3 (23.0–29.5)77.5 ± 1.6 (75.0–79.0)
Vagina / BD or spicules / ABD?1.30.52.1 ± 0.1 (1.9–2.2)
Rectum or gubernaculum length??29.6 ± 3.2 (27.5–35.0)12.3 ± 0.6 (11.5–12.5)
Rectum / ABD or apophysis length??0.8 ± 0.1 (0.8–0.9)27.4 ± 1.3 (26.0–28.5)
Caudal gland openings from tail terminus??2.9 ± 0.7 (2.0–3.5)2.0
- - -Female - - -Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube -248–280 µm -long, situated to either right (n = 1) or left (n = 4) of intestine; posterior genital tube -224–259 µm -long, situated to either left (n = 1) or right (n = 4) of intestine. Vulva a transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; -pars refringens vaginae -absent. Sack-like spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. - - - -Fig. 3. - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen. et comb. nov. -(SMNH-169255). -A -. ♂, entire specimen. -B -. ♀, entire specimen. -C -. Anterior testis. -D -. Posterior testis. Scale bars: A–B = 100 µm; C–D = 50 µm. - - - -Male - - -Reproductive system diorchic, testes opposed; anterior testis -162–286 µm -long, outstretched and posterior testis -117–162 µm -long, reflexed. Spicules paired and symmetrical, strongly curved, with ovoid manubrium and subcylindrical shaft. Gubernaculum plate-like, with pair of strong, closely set caudal apophyses variable in shape ( -Fig. 4 -C–E). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length (often difficult to observe). - - - - - -Remarks - - - -Despite the fact that the original description by -Southern (1914) -is rather brief, the present population closely resembles the -type -specimens in most morphological and morphometric features, including the shape of the refractive plates underlying the amphids (described as “shield shaped” with “front and lateral walls are notched”). Spicules are slightly shorter in the -type -specimens ( -66 µm -vs -75–79 µm -in recent specimens). - - - -Fig. 4. - -Neodiplopeltula incisa -( -Southern, 1914 -) - -gen. et comb. nov. -(SMNH-169255). -A -. Male anterior end, lateral view. -B -. Female anterior end, lateral view. -C -. Spicules and gubernaculum. -D–E -. Variability in the shape of gubernaculum apophyses in the same specimen (arrows). Scale bars = 10 µm. - - - - -Neodiplopeltula barentsi -( -Steiner, 1916 -) - -gen. et comb. nov. -Figs 5–7 -, -14–15 -; -Table 3 - - -Diagnosis -(based on combined data) - - - -Neodiplopeltula barentsi - -gen. et comb. nov. -is characterised by a -0.94–1.67 mm -long body; refractive plates underlying cephalic cuticle around amphids present, elongated ovoid in shape, not joined, -23.5– 32 µm -long and -12–16 µm -wide; cephalic setae -3.5–7.5 µm -long; amphidial fovea -21–32 µm -long and -4–7 µm -wide; oral opening 1–2 from anterior end, posterior to cephalic setae bases; secretory-excretory - - - -Fig. 5. - -Neodiplopeltula barentsi -( -Steiner, 1916 -) - -gen. et comb. nov. -(SMNH-169268). -A -. Male anterior end, surface view. -B -. Anterior part of female reproductive system. -C -. Female anterior end, surface view. -D -. Female pharyngeal region, optical median section. -E -. Male tail. -F -. Female tail. Scale bar = 20 µm. - - - -pore located opposite to posterior part of pharynx; tail 1.6–3.3 anal body diameters long; vagina with epiptygmata; spicules -45–73 µm -long. - - - - - - -Material examined - - - - -SWEDEN -: -1 ♀ -, -1 ♂ -, -Skagerrak -, -58°20′06″ N -, -11°09′24″ E -, muddy sediment at a deep of - - -53 m - - -, - -9 Aug. 2011 - -, -O. Holovachov -leg. (SMNH-169264); - -1 ♀ -, -1 ♂ -, -Skagerrak -, -58°22′19″ N -, -11°04′55″ E -, muddy sediment at a deep of - - -55– -70 m - - -, - -9 Aug. 2011 - -, -O. Holovachov -leg. (SMNH-169266); - - -3 ♀♀ -, -1 ♂ -, -Gullmarn Fjord near Östersidan -, -58°15′09″ N -, -11°27′54″ E -, shells, gravel sand and mud, - -14 Aug. 2014 - -, -O. Holovachov -leg. (SMNH-169268); - - -1 ♀ -lectotype -of - - -Diplopeltis -asymmetricus - -, -Öresund - -, west of -Valgrundet -, at a deep of - - -30– -36 m - - -, - - -Zostera - -and - -Echinocardium - - -, - -7 Aug. 1926 - -( -SMNH -Type-9015). - - - - -GREENLAND -: -1 ♀ -, -holotype -of - -Diplopeltis ovalis -, Godhavn - -, mud, -17 Aug. 1926 -, Reisinger and Steinböck leg. ( -NHMD -102171) - -. - - - - - -Description - - - -Adult - - -Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body, except for visually smooth labial region and terminal part of tail (striation can be observed under LM but very fine and shallow and cannot be measured with confidence); longitudinal striation not observed. Somatic sensilla visible along pharyngeal region (cervical sensilla, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates elongated ovoid in shape (plate edges not incised), not connected with each other on ventral and on dorsal sides. Inner labial sensilla indistinct. Outer labial sensilla papilliform, located on anterior surface of lips. Cephalic sensilla setiform, bases of dorsosublateral setae located posterior to oral opening. Cervical sensilla papilliform, arranged in four sublateral rows starting at level with middle of amphid and ending at level with posterior edge of refractive plate, one or two per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch usually 0.5–2.0 µm longer than ventral branch (in two specimens ventral branch 0.5–1.0 µm longer than dorsal branch). Oral opening shifted towards dorsal side of body. Stoma barrel-shaped: cheilostom broad; gymnostom subcylindrical, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia ovoid, entirely embedded into intestinal tissue. Secretoryexcretory system present; secretory-excretory pore along ventral body line opposite to 3/5 -th -of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail conoid with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. - - - -Fig. 6. - -Neodiplopeltula barentsi -( -Steiner, 1916 -) - -gen. et comb. nov. -(SMNH-169268). -A -. ♂, entire specimen. -B -. ♀, entire specimen. -C -. Anterior testis. -D -. Posterior testis. Scale bars: A–B = 100 µm; C–D = 50 µm. - - - - -Table 3 -(continued on next page). Measurements (in µm) of - -Neodiplopeltula barentsi -( -Steiner, 1916 -) - -gen. et comb. nov. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - - -D. ovalis - - - -D. asymmetricus - - - - -D. onustus -sensu Sergeeva (1974) - - - - -data for - -D. cuspidiboja - - - -recent specimens -
♂♀ holotype♀ lectotype♀♂♂5 ♀♀3 ♂♂
Body length152612289701057114711471207 ± 258 (935–1532)1487 ± 285 (1159–1670)
Body diameter (BD)?616539454452 ± 13 (41–74)55 ± 15 (38–65)
Pharynx length205197159169180175176 ± 11 (168–193)182 ± 15 (165–195)
Tail length122956473796784 ± 8 (76–96)98 ± 9 (91–108)
Anal body diameter (ABD)?384022343235 ± 6 (29–43)41 ± 6 (34–46)
a?20.114.926.825.52623.3 ± 4.0 (19.1–29.6)27.7 ± 2.9 (25.1–30.8)
b7.46.26.16.36.476.8 ± 1.1 (5.5–8.0)8.1 ± 1.0 (7.0–8.9)
c12.512.915.214.513.61714.3 ± 2.0 (12.0–16.0)15.2 ± 2.6 (12.7–17.9)
c’?2.51.63.32.32.12.4 ± 0.3 (2.2–2.8)2.4 ± 0.3 (2.1–2.7)
V(%) / T(%)?60.764.262.3??61.5 ± 1.9 (59.6–63.5)48.1 ± 4.7 (43.6–53.1)
Labial region diameter?161812171714.9 ± 0.8 (14.0–16.0)15.0 ± 1.1 (14.0–16.0)
Cephalic setae length???7.57.545.5 ± 1.7 (3.5–7.5)7.4 ± 0.4 (7.0–7.5)
Subdorsal cephalic setae from anterior end?????116.6 ± 0.6 (5.5–7.0)7.6 ± 0.7 (7.0–8.0)
Subventral cephalic setae from anterior end?????96.5 ± 0.6 (5.5–7.0)7.1 ± 0.4 (7.0–7.5)
Cephalic setae length / labial region diam.??????0.4 ± 0.1 (0.2–0.5)0.5
Anterior end of shield from anterior end?10????7.5 ± 0.6 (7.0–8.0)10.0 ± 1.5 (9.0–11.0)
Refractive plate length?25???3225.7 ± 1.5 (23.5–27.0)24.5 ± 0.5 (24.0–25.0)
Refractive plate width?12???12.513.9 ± 1.3 (12.5–16.0)15.2 ± 1.0 (14.5–16.0)
Anterior end of amphid from anterior end?76??65.5 ± 0.7 (5.0–6.0)6.4 ± 1.1 (5.5–7.5)
Dorsal amphid branch length?262629293223.3 ± 2.4 (21.0–25.5)22.5 ± 1.1 (21.5–23.5)
Ventral amphid branch length?2324??3022.6 ± 1.6 (21.0–24.0)21.9 ± 0.4 (21.5–22.0)
Amphid width?676675.1 ± 0.6 (4.0–5.5)5.8 ± 0.4 (5.5–6.0)
Amphid length / width?4.33.74.84.84.64.6 ± 0.9 (3.8–6.2)3.9 ± 0.3 (3.7–4.3)
Anterior edge of stoma from anterior end?11???1.1 ± 0.4 (1.0–1.5)1.4 ± 0.7 (1.0–2.0)
- - - -Table 3 -(continued). - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - - -D. ovalis - - - -D. asymmetricus - - - - -D. onustus -sensu Sergeeva (1974) - - - - -data for - -D. cuspidiboja - - - -recent specimens -
Stoma width??????5.4 ± 0.6 (5.0–6.0)5.3 ± 0.4 (5.0–5.5)
Nerve ring from anterior end???84967290 ± 5 (84–97)96 ± 10 (86–104)
Nerve ring from anterior end (% pharynx)?????4151.1 ± 1.0 (50.3–52.7)52.7 ± 0.8 (51.7–53.2)
Secretory-excretory pore from ant. end?115???104109 ± 3 (106–112)110 ± 7 (103–115)
Secr.-excr. pore from ant. end (% pharynx)?58.4???6062.2 ± 2.8 (58.0–65.5)60.3 ± 1.6 (59.1–62.1)
Vagina or spicules length????455818.2 ± 1.1 (17.0–19.5)63.8 ± 8.2 (57.0–73.0)
Vagina / BD or spicules / ABD????1.1?0.4 ± 0.1 (0.3–0.5)1.6 ± 0.3 (1.3–1.8)
Rectum or gubernaculum length?????2023.7 ± 5.0 (20.5–32.0)11.0 ± 0.7 (10.0–11.5)
Rectum / ABD or apophysis length??????0.7 ± 0.1 (0.6–0.7)18.6 ± 2.4 (16.0–20.5)
Caudal gland openings from tail terminus?????32.0 ± 0.6 (1.0–2.0)1.9 ± 0.7 (1.0–2.0)
- - -Female - - -Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube -179–269 µm -long, situated to either right (n = 3) or left (n = 2) of intestine; posterior genital tube -173–286 µm -long, situated to either left (n = 3) or right (n = 2) of intestine. Vulva transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; -pars refringens vaginae -absent. Sacklike spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. - - -Male - - -Reproductive system diorchic, testes opposed; anterior testis -145–314 µm -long, outstretched and posterior testis -117–207 µm -long, reflexed. Spicules paired and symmetrical, strongly curved, with weakly defined elongated manubrium and shaft, cylindrical along most of its length. Gubernaculum plate-like, with pair of strong, closely set caudal apophyses variable in shape ( -Fig. 7 -C–E). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length (often difficult to observe). - - - - - -Remarks - - - -The original description of this species by -Steiner (1916) -is rather short, with a few measurements given. The present population has a shorter pharynx and tail compared to the -type -specimen, but in all other respects both match very well, including the position of the oral opening and the shape of the underlying refractive plates. - - - -Fig. 7. - -Neodiplopeltula barentsi -( -Steiner, 1916 -) - -gen. et comb. nov. -(SMNH-169266 and SMNH-169268). -A -. Female anterior end, lateral view. -B -. Male anterior end, lateral view. -C -. Spicules and gubernaculum. -D–E -. Variability in the shape of thegubernaculum apophyses in the same specimen (arrows). Scale bars = 10 µm. - - - - -Neodiplopeltula onusta -( -Wieser, 1956 -) - -gen. et comb. nov. -Figs 8–10 -; -Table 4 - - -Diagnosis -(based on combined data) - - - -Neodiplopeltula onusta - -gen. et comb. nov. -is characterised by a -0.99–1.48 mm -long body; refractive plates underlying cephalic cuticle around amphids present and joined, -32.5–48 µm -long and -18.5–24 µm -wide; cephalic setae -3–6.5 µm -long; amphidial fovea -32–44 µm -long and -5–8 µm -wide; oral opening 2–5 from anterior end, at level with cephalic setae bases; secretory-excretory pore located opposite to posterior part of pharynx; tail 1.4–2.3 anal body diameters long; vagina with epiptygmata; spicules -52–77 µm -long. - - - - -Fig. 8. - -Neodiplopeltula onusta -( -Wieser, 1956 -) - -gen. et comb. nov. -(SMNH-169282). -A -. Male anterior end, surface view. -B -. Female anterior end, surface view. -C -. Female pharyngeal region, optical median section. -D -. Anterior part of female reproductive system. -E -. Male tail. -F -. Female tail. Scale bar = 20 µm. - - - - -Table 4 -(continued on next page). Measurements (in µm) of - -Neodiplopeltula onusta -( -Wieser, 1956 -) - -gen. et comb. nov. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - - -data for - -Diplopeltula tchesunovi - - - -recent specimens -
♀4 ♀♀3 ♂♂4 ♀♀3 ♂♂
Body length9901348–14811003–13231064 ± 104 (947–1199)1056 ± 50 (999–1094)
Body diameter (BD)?65–8848–5847 ± 9 (38–55)46 ± 7 (39–53)
Pharynx length?201–223172–212170 ± 17 (149–187)174 ± 15 (159–189)
Tail length?75–7765–8158 ± 7 (51–68)74 ± 12 (65–88)
Anal body diameter (ABD)?44–5237–4134 ± 3 (32–37)37 ± 3 (34–39)
a2617–2321–2323.6 ± 5.9 (19.3–31.9)23.4 ± 4.4 (20.5–28.4)
b5.16.3–7.35.9–6.36.3 ± 0.8 (5.3–7.2)6.1 ± 0.3 (5.8–6.3)
c14.217.5–19.212.5–17.418.7 ± 3.1 (15.2–21.4)14.6 ± 2.8 (11.4–16.9)
c’1.7??1.7 ± 0.3 (1.4–2.2)2.0 ± 0.2 (1.8–2.3)
V(%) / T(%)65.465.1–67.6?66.1 ± 1.5 (64.7–68.0)41.0 ± 7.9 (35.4–46.6)
Labial region diameter1512–16.514–1511.2 ± 1.7 (9.0–13.0)12.0 ± 0.8 (11.0–12.5)
Cephalic setae length6.53.5–5.43.5–4.54.0 ± 1.2 (3.0–5.5)3.9 ± 0.8 (3.5–5.0)
Subdorsal cephalic setae from anterior end8??6.4 ± 0.7 (5.5–7.0)7.4 ± 1.1 (6.0–8.0)
Subventral cephalic setae from anterior end???5.9 ± 0.7 (5.0–6.0)6.2 ± 0.7 (5.5–7.0)
Cephalic setae length / labial region diam.???0.4 ± 0.1 (0.3–0.5)0.3 ± 0.1 (0.3–0.4)
Anterior end of shield from anterior end???9.2 ± 0.4 (9.0–10.0)10.1 ± 0.4 (10.0–10.5)
Refractive plate length?45–4841–4736.1 ± 3.2 (32.5–38.0)35.0 ± 1.1 (34.0–36.0)
Refractive plate width?20–2419–2021.6 ± 1.5 (19.0–23.0)20.0 ± 1.4 (18.5–21.5)
Anterior end of amphid from anterior end?4–75–75.9 ± 0.9 (5.0–7.0)7.4 ± 0.8 (7.0–8.0)
Dorsal amphid branch length4041–4436–4336.2 ± 2.6 (32.5–38.0)35.9 ± 0.7 (35.0–36.5)
Ventral amphid branch length4039–4334–4135.0 ± 2.5 (32.0–37.0)34.3 ± 1.1 (33.0–35.0)
Amphid width85–75–66.6 ± 0.9 (5.5–7.5)6.7 ± 0.4 (6.0–7.0)
- - - -Table 4 -(continued). - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
-original description - - -data for - -Diplopeltula tchesunovi - - - -recent specimens -
Amphid length / width???5.6 ± 0.7 (5.0–6.6)5.4 ± 0.2 (5.2–5.7)
Anterior edge of stoma from anterior end?552.8 ± 0.8 (2.0–3.5)3.7 ± 1.1 (3.0–5.0)
Stoma length???13.6 ± 0.7 (13.0–14.5)13.6 ± 0.4 (13.0–14.0)
Stoma width???4.8 ± 0.6 (4.0–5.5)5.0
Nerve ring from anterior end?96–10690–10392 ± 6 (84–96)95 ± 6 (91–102)
Nerve ring from anterior end (% pharynx)???54.4 ± 2.9 (50.5–56.8)54.3 ± 2.6 (52.0–57.1)
Secretory-excretory pore from ant. end115110–12585–87104 ± 8 (95–112)107 ± 4 (103–112)
Secr.-excr. pore from ant. end (% pharynx)66??61.1 ± 2.9 (57.0–63.5)61.7 ± 2.9 (59.3–64.8)
Vagina or spicules length?35–4062–7219.7 ± 2.7 (16.5–23.0)63.7 ± 11.8 (56.5–77.0)
Vagina / BD or spicules / ABD??1.3–1.50.4 ± 0.1 (0.3–0.6)1.7 ± 0.3 (1.5–2.0)
Rectum or gubernaculum length??15–2024.1 ± 3.6 (21.0–28.0)10.0 ± 0.5 (10.0–10.5)
Rectum / ABD or apophysis length???0.7 ± 0.1 (0.6–0.9)16.1 ± 1.1 (15.0–17.0)
Caudal gland openings from tail terminus???terminalterminal
- - - - - -Material examined - - - -SWEDEN -: -1 ♀ -, Skagerrak, 58°19′15.6″–20.9″ N, 10°29′33.5″–34.0″ E, soft bottom at a deep of -352–374 m -, -10 Sep. 2012 -, “Inventering Bratten” leg. (SMNH-169284); 1 ♂, Skagerrak, 58°23′00.8″– 22′00.8″ N, 10°20′28.8″–38.3″ E, soft bottom at a deep of -390–428 m -, -10 Sep. 2012 -, “Inventering Bratten” leg. (SMNH-169280); 1 ♂, Skagerrak, 58°28′21.2″–19.2″ N, 10°29′35.6″–43.6″ E, soft bottom at a deep of -248–316 m -, -11 Sep. 2012 -, “Inventering Bratten” leg. (SMNH-169281); -3 ♀♀ -, 1 ♂, Skagerrak, 58°27′36.7″–43.3″ N, 10°32′52.0″–59.4″ E, soft bottom at a deep of -232–240 m -, -12 Sep. 2012 -, “Inventering Bratten” leg. (SMNH-169282 – SMNH-169283). - - - - - - -Description - - - -Adult - - -Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body, except for visually smooth labial region and terminal part of tail (striation can be observed under LM but very fine and shallow and cannot be measured with confidence); longitudinal striation not observed.Somatic sensilla visible along pharyngeal region (cervical setae, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates connected with each other on ventral and on dorsal sides. Inner labial sensilla indistinct. Outer labial sensilla papilliform, located on anterior surface of lips. Cephalic sensilla setiform, bases of dorsosublateral setae located at level with oral opening. Cervical sensilla papilliform, arranged in four sublateral rows at level with amphid, one or two per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch 1.0–2.0 µm longer than ventral branch. Oral opening shifted towards dorsal side of body. Stoma subcylindrical: cheilostom broad; gymnostom barrel-shaped, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Secretory-excretory system present; secretory-excretory pore located along ventral body line opposite to 3/5 -th -of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail conoid with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. - - - -Fig. 9. - -Neodiplopeltula onusta -( -Wieser, 1956 -) - -gen. et comb. nov. -(SMNH-169282). -A -. ♀, entire specimen. -B -. ♂, entire specimen. -C -. Testes. Scale bars: A–B = 100 µm; C = 50 µm. - - - -Female - - -Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube -109–180 µm -long, situated to either right (n = 3) or left (n = 1) of intestine; posterior genital tube -109–145 µm -long, situated to either left (n = 3) or right (n = 1) of intestine. Vulva transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; -pars refringens vaginae -absent. Sack-like spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. - - - -Fig. 10. - -Neodiplopeltula onusta -( -Wieser, 1956 -) - -gen. et comb. nov. -(SMNH-169282). -A -. Male anterior end, lateral view. -B -. Male stoma, lateral view. -C -. Female stoma, lateral view. -D -. Male anterior end, dorsal view. -E–G -. Variability in the shape of spicules and gubernaculum in different specimens. Scale bars = 10 µm. - - - -Male - - -Reproductive system diorchic, testes opposed; anterior testis outstretched and posterior testis reflexed (poorly discernible in most specimens due to highly granular and dark content of overlapping intestine and cannot be measured). Spicules paired and symmetrical, strongly curved, with elongated ovoid manubrium and subcylindrical shaft. Gubernaculum plate-like, with pair of strong closely set caudal apophyses variable in shape ( -Fig. 10 -E–G). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length. - - - - - -Remarks - - - -The single female used by -Wieser (1956) -to describe this species has a slightly broader labial region and longer cephalic setae than the population from -Sweden -, but the specimens described under the name - -Diplopeltula tchesunovi - -fill this gap. The position of the oral opening is at level with the cephalic setae bases and the shape of the refractive plates underlying the cephalic cuticle around the amphids (joined along the dorsal and ventral body sides) in both -type -female and recent specimens are identical and confirm the conspecificity. - - - - \ No newline at end of file diff --git a/data/03/AC/5D/03AC5D376848FFF5FE7C574DBBD2FCA1.xml b/data/03/AC/5D/03AC5D376848FFF5FE7C574DBBD2FCA1.xml new file mode 100644 index 00000000000..400935b9596 --- /dev/null +++ b/data/03/AC/5D/03AC5D376848FFF5FE7C574DBBD2FCA1.xml @@ -0,0 +1,565 @@ + + + +Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) + + + +Author + +Holovachov, Oleksandr +89D30ED8-CFD2-42EF-B962-30A13F97D203 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +oleksandr.holovachov@nrm.se + + + +Author + +Boström, Sven +528300CC-D0F0-4097-9631-6C5F75922799 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +sven.bostrom@nrm.se + +text + + +European Journal of Taxonomy + + +2018 + +2018-08-29 + + +458 + + +1 +34 + + + +journal article +22251 +10.5852/ejt.2018.458 +4a6349ea-1928-45b5-855f-bd13b697bb49 +3826987 +16C565AB-22A6-496F-AE95-0A876066F958 + + + + + + +Neodiplopeltula incisa +( +Southern, 1914 +) + +gen et. comb. nov. + + + + + +Figs 1–4 +; +Table 2 + + + + +Diagnosis +(based on combined data) + + + +Neodiplopeltula incisa + +gen. et comb. nov. +is characterised by a +1.52–2.24 mm +long body; refractive plates underlying cephalic cuticle around amphids present, irregular in shape, not joined, +28–33 µm +long and +21–25 µm +wide; cephalic setae +8–14 µm +long; amphidial fovea +22–28 µm +long and +5.5–7 µm +wide; oral opening +1–3 µm +from anterior end, posterior to cephalic setae bases; secretory-excretory pore located opposite to posterior part of pharynx; tail 2.5–3.9 anal body diameters long; vagina with epiptygmata; spicules +66–79 µm +long. + + + + + + +Material examined + + + + + +SWEDEN +: +4 ♀♀ +, 3 ♂♂, Skagerrak, +58°12′37″ N +, +11°18′53″ E +, shells and sand at a deep of +15–22 m +, +10 Oct. 2012 +, O. Holovachov leg. (SMNH-169255); + + +1 ♀ +, 1 ♂, Skagerrak, +58°20′21″ N +, +11°12′42″ E +, coarse shell sand at a deep of +14–17 m +, +19 Aug. 2014 +, O. Holovachov leg. (SMNH-169258). + + + + + +Fig. 1. + +Neodiplopeltula incisa +( +Southern, 1914 +) + +gen. et comb. nov. +(SMNH-169255). +A +. Anterior part of female reproductive system. +B +. Male tail. +C +. Female pharyngeal region, optical median section. +D +. Female anterior end, surface view. +E +. Male anterior end, surface view. +F +. Female tail. Scale bar = 20 µm. + + + + + +Description + + + +Adult + + +Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body as seen under SEM (striation can be observed under LM but very fine and shallow and cannot be measured with confidence), except for visually smooth labial region and terminal part of tail; longitudinal striation not observed under the light microscope, but distinct under the scanning electron microscope, covers cuticle over entire body length. Somatic sensilla visible along pharyngeal region (cervical setae, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates irregular in shape (with incised edges), not connected with each other on ventral and dorsal sides. Inner labial sensilla small pore-like, located on anterior surface of lips, discernible under SEM only. Outer labial sensilla papilliform, located on anterior surface of lips, clearly visible under SEM only. Cephalic sensilla setiform, bases of dorsosublateral setae located posterior to oral opening. Cervical sensilla papilliform, arranged in four sublateral rows starting at level with middle of amphid and ending at level with posterior edge of refractive plate, two or three per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch +0.5–3.5 µm +longer than ventral branch. Oral opening shifted towards dorsal side of body. Stoma barrel-shaped: cheilostom broad; gymnostom barrel-shaped, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia ovoid, almost entirely embedded into intestinal tissue. Secretory-excretory system present; secretory-excretory pore located along ventral body line opposite to posterior 3/5 +th +of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail cylindro-conical with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. + + + +Fig. 2. + +Neodiplopeltula incisa +( +Southern, 1914 +) + +gen. et comb. nov. +, SEM micrographs. +A–C +, +F–G +. ♂. +D–E +. ♀. +A +. Male anterior end, semi-apical view, dorsal side to the left. +B +. Male labial region, semiapical view, dorsal side down (arrows point at inner labial sensilla). +C +. Male anterior end, right lateral view. +D +. Vulval region. +E +. Tail terminus. +F +. Male cloacal region, lateral view. +G +. Male cloacal region, ventrosubventral view. Scale bars: A, C–D, F–G = 10 µm; B, E = 2 µm. + + + + +Table 2 +(continued on next page). Measurements (in µm) of + +Neodiplopeltula incisa +( +Southern, 1914 +) + +gen. et comb. nov. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + +recent specimens +
♀2 ♂♂5 ♀♀4 ♂♂
Body length20202100–22401740 ± 176 (1515–2001)1748 ± 131 (1591–1908)
Body diameter (BD)6253–5848 ± 7 (42–59)45 ± 9 (34–53)
Pharynx length270250–270204 ± 24 (186–245)216 ± 31 (178–254)
Tail length124124–136122 ± 10 (106–135)133 ± 13 (116–147)
Anal body diameter (ABD)455035 ± 3 (32–39)37 ± 1 (37–39.0)
a36.738.6–39.636.9 ± 4.3 (30.0–40.8)39.9 ± 6.1 (32.8–46.5)
b7.58.3–8.48.6 ± 0.7 (7.7–9.6)8.2 ± 0.6 (7.5–8.9)
c16.316.5–1714.4 ± 1.6 (12.4–16.8)13.1 ± 0.4 (12.9–13.7)
c’2.82.53.5 ± 0.3 (2.9–3.8)3.6 ± 0.3 (3.2–3.9)
V(%) / T(%)61?62.7 ± 0.4 (62.1–62.9)45.8 ± 5.1 (40.4–51.6)
Labial region diameter??19.6 ± 1.6 (18.0–22.0)20.9 ± 1.1 (20.0–22.0)
Cephalic setae length??10.6 ± 2.2 (8.0–14.0)11.0 ± 1.0 (10.5–12.0)
Subdorsal cephalic setae from anterior end??7.2 ± 2.0 (5.5–10.5)6.4 ± 0.8 (5.5–7.0)
Subventral cephalic setae from anterior end??7.2 ± 2.0 (5.5–10.5)6.4 ± 0.8 (5.5–7.0)
Cephalic setae length / labial region diam.??0.5 ± 0.1 (0.4–0.8)0.5
Anterior end of plate from anterior end??9.5 ± 1.0 (8.0–10.5)10.0
Refractive plate length??31.4 ± 2.1 (28.0–33.0)32.0 ± 1.1 (31.0–33.0)
Refractive plate width??23.6 ± 2.0 (21.0–25.0)23.2 ± 0.8 (23.0–24.0)
Anterior end of amphid from anterior end??6.2 ± 1.2 (4.0–7.0)6.4 ± 0.8 (5.5–7.0)
Dorsal amphid branch length??27.3 ± 1.8 (25.5–30.0)28.5 ± 1.6 (27.6–30.4)
Ventral amphid branch length??25.4 ± 2.3 (22.0–27.0)27.6 ± 0.7 (26.9–28.3)
Amphid width??5.7 ± 0.3 (5.5–6.0)6.2 ± 0.7 (5.5–6.9)
Amphid length / width??4.8 ± 0.2 (4.6–5.0)4.6 ± 0.3 (4.4–5.0)
+ + + +Table 2 +(continued). + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + +recent specimens +
Stoma length??14.4 ± 1.8 (12.5–17.0)16.3 ± 0.8 (16.0–17.0)
Stoma width??5.7 ± 0.3 (5.5–6.0)6.0 ± 0.4 (5.5–6.0)
Nerve ring from anterior end?111–115102 ± 10 (94–114)106 ± 12 (91–120)
Nerve ring from anterior end (% pharynx)?42.6–44.449.7 ± 3.1 (46.5–53.8)49.4 ± 1.7 (47.1–51.3)
Secretory-excretory pore from ant. end??118 ± 13 (108–140)125 ± 19 (99–143)
Secr.-excr. pore from ant. end (% pharynx)??58.2 ± 1.4 (57.0–60.6)57.8 ± 2.2 (55.8–60.4)
Vagina or spicules length?6624.8 ± 3.3 (23.0–29.5)77.5 ± 1.6 (75.0–79.0)
Vagina / BD or spicules / ABD?1.30.52.1 ± 0.1 (1.9–2.2)
Rectum or gubernaculum length??29.6 ± 3.2 (27.5–35.0)12.3 ± 0.6 (11.5–12.5)
Rectum / ABD or apophysis length??0.8 ± 0.1 (0.8–0.9)27.4 ± 1.3 (26.0–28.5)
Caudal gland openings from tail terminus??2.9 ± 0.7 (2.0–3.5)2.0
+ + +Female + + +Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube +248–280 µm +long, situated to either right (n = 1) or left (n = 4) of intestine; posterior genital tube +224–259 µm +long, situated to either left (n = 1) or right (n = 4) of intestine. Vulva a transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; +pars refringens vaginae +absent. Sack-like spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. + + + +Fig. 3. + +Neodiplopeltula incisa +( +Southern, 1914 +) + +gen. et comb. nov. +(SMNH-169255). +A +. ♂, entire specimen. +B +. ♀, entire specimen. +C +. Anterior testis. +D +. Posterior testis. Scale bars: A–B = 100 µm; C–D = 50 µm. + + + +Male + + +Reproductive system diorchic, testes opposed; anterior testis +162–286 µm +long, outstretched and posterior testis +117–162 µm +long, reflexed. Spicules paired and symmetrical, strongly curved, with ovoid manubrium and subcylindrical shaft. Gubernaculum plate-like, with pair of strong, closely set caudal apophyses variable in shape ( +Fig. 4 +C–E). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length (often difficult to observe). + + + + + +Remarks + + + +Despite the fact that the original description by +Southern (1914) +is rather brief, the present population closely resembles the +type +specimens in most morphological and morphometric features, including the shape of the refractive plates underlying the amphids (described as “shield shaped” with “front and lateral walls are notched”). Spicules are slightly shorter in the +type +specimens ( +66 µm +vs +75–79 µm +in recent specimens). + + + + \ No newline at end of file diff --git a/data/03/AC/5D/03AC5D37684CFFFFFD9856A6BBE7FAED.xml b/data/03/AC/5D/03AC5D37684CFFFFFD9856A6BBE7FAED.xml index 09b064978ae..6a77679d7ba 100644 --- a/data/03/AC/5D/03AC5D37684CFFFFFD9856A6BBE7FAED.xml +++ b/data/03/AC/5D/03AC5D37684CFFFFFD9856A6BBE7FAED.xml @@ -1,54 +1,54 @@ - - - -Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) + + + +Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) - - -Author + + +Author -Holovachov, Oleksandr -89D30ED8-CFD2-42EF-B962-30A13F97D203 -Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. -oleksandr.holovachov@nrm.se +Holovachov, Oleksandr +89D30ED8-CFD2-42EF-B962-30A13F97D203 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +oleksandr.holovachov@nrm.se - - -Author + + +Author -Boström, Sven -528300CC-D0F0-4097-9631-6C5F75922799 -Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. -sven.bostrom@nrm.se +Boström, Sven +528300CC-D0F0-4097-9631-6C5F75922799 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +sven.bostrom@nrm.se -text - - -European Journal of Taxonomy +text + + +European Journal of Taxonomy - -2018 - -2018-08-29 + +2018 + +2018-08-29 - -458 + +458 - -1 -34 + +1 +34 -journal article -22251 -10.5852/ejt.2018.458 -4a6349ea-1928-45b5-855f-bd13b697bb49 -3826987 -16C565AB-22A6-496F-AE95-0A876066F958 +journal article +22251 +10.5852/ejt.2018.458 +4a6349ea-1928-45b5-855f-bd13b697bb49 +3826987 +16C565AB-22A6-496F-AE95-0A876066F958 - - + + Genus @@ -58,6 +58,8 @@ Genus gen. nov. + + urn:lsid:zoobank.org:act: @@ -65,7 +67,7 @@ urn:lsid:zoobank.org:act: - + @@ -125,14 +127,14 @@ partim (note #2) -Cuticle transversely striated; striae visibly smooth under light microscope, may have fine longitudinal incisures visible under SEM. Lateral alae absent. Body pores and epidermal glands absent. Somatic sensilla present, most prominent along anterior part of pharyngeal region and on tail. Labial region bluntly rounded; lips fused. Six inner labial sensilla small, pore-like, located on anterior surface of lips, discernible under SEM only. Six outer labial sensilla papilliform, located on anterior surface of lips. Four cephalic sensilla setiform; their bases located at base of labial region, at level with anteriormost part of amphid. Bases of dorsosublateral setae often located more posteriorly than bases of ventrosublateral setae. Subcephalic sensilla absent. Cervical sensilla present in some species, setiform or papilliform, arranged in dorsosublateral and ventrosublateral rows at level with amphid and subcuticular periamphideal plates. Deirid and ocelli absent. Amphids in some species on strongly refractive lateral subcuticular periamphideal plates that can be connected together on ventral and dorsal sides forming a ‘cephalic framework’. Amphidial fovea loop-shaped (inverted U-shaped), dorsal branch often longer than ventral branch; both branches of amphideal fovea closely adjacent. Secretory- excretory system present; renette cell located opposite to posterior part of pharynx or anterior part of intestine. Secretory-excretory ampulla present, located at level of pharynx. Cuticularised secretoryexcretory duct very short, opens to exterior either anterior or posterior to nerve ring level. Oral opening apical or shifted towards dorsal side of body. Buccal cavity usually well developed, subcylindrical and often asymmetrical: cheilostom usually broad, flexible; gymnostom subcylindrical, with weakly cuticularised walls; stegostom short conoid, its lining is uniform with lining of pharynx.Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided into distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia glandular, variable in shape and size, its posterior part embedded in intestinal tissue. Female reproductive system didelphic-amphidelphic, with equally developed branches, ovaries outstretched. Spermatheca present. Vulva equatorial or postquatorial. Vagina straight; +Cuticle transversely striated; striae visibly smooth under light microscope, may have fine longitudinal incisures visible under SEM. Lateral alae absent. Body pores and epidermal glands absent. Somatic sensilla present, most prominent along anterior part of pharyngeal region and on tail. Labial region bluntly rounded; lips fused. Six inner labial sensilla small, pore-like, located on anterior surface of lips, discernible under SEM only. Six outer labial sensilla papilliform, located on anterior surface of lips. Four cephalic sensilla setiform; their bases located at base of labial region, at level with anteriormost part of amphid. Bases of dorsosublateral setae often located more posteriorly than bases of ventrosublateral setae. Subcephalic sensilla absent. Cervical sensilla present in some species, setiform or papilliform, arranged in dorsosublateral and ventrosublateral rows at level with amphid and subcuticular periamphideal plates. Deirid and ocelli absent. Amphids in some species on strongly refractive lateral subcuticular periamphideal plates that can be connected together on ventral and dorsal sides forming a ‘cephalic framework’. Amphidial fovea loop-shaped (inverted U-shaped), dorsal branch often longer than ventral branch; both branches of amphideal fovea closely adjacent. Secretoryexcretory system present; renette cell located opposite to posterior part of pharynx or anterior part of intestine. Secretory-excretory ampulla present, located at level of pharynx. Cuticularised secretoryexcretory duct very short, opens to exterior either anterior or posterior to nerve ring level. Oral opening apical or shifted towards dorsal side of body. Buccal cavity usually well developed, subcylindrical and often asymmetrical: cheilostom usually broad, flexible; gymnostom subcylindrical, with weakly cuticularised walls; stegostom short conoid, its lining is uniform with lining of pharynx.Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided into distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Cardia glandular, variable in shape and size, its posterior part embedded in intestinal tissue. Female reproductive system didelphic-amphidelphic, with equally developed branches, ovaries outstretched. Spermatheca present. Vulva equatorial or postquatorial. Vagina straight; pars proximalis vaginae encircled by large sphincter muscle; pars refringens vaginae absent, epiptygmata present or absent. Male reproductive system diorchic, testes opposed (anterior testis outstretched and posterior testis reflexed). Spicules symmetrical, arcuate; gubernaculum present, usually with strongly developed apophyses. Supplements, precloacal and postcloacal sensilla absent. Three caudal glands present, open via three separate openings, their cells and nuclei incaudal. Spinneret absent. - + Etymology @@ -149,6 +151,8 @@ The genus name is composed of a prefix ‘neo–’, meaning ‘new’ and . + + Relationships @@ -213,6 +217,8 @@ is also considered species inquirenda et incertae sedis for the same reasons (incomplete description and unclear systematic position). + + Valid species @@ -518,13 +524,13 @@ Boucher & Helléouët, 1977 (note #12) + + Nomenclatorial changes and notes - - 1) The description of @@ -625,8 +631,6 @@ and synonimised with gen. et comb. nov. - - 4) The lectotype @@ -690,7 +694,7 @@ is identical to gen. et comb. nov. in the position of the oral opening, size and shape of refractive plates and the great majority of quantitative characters (see -Table 3 +Table 3 ), except for a slightly longer amphid ( 32 µm vs @@ -771,7 +775,7 @@ is identical to D. onusta in all qualitative and quantitative characters ( -Table 4 +Table 4 ), with the exception of longer refractive plates ( 41–48 µm vs diff --git a/data/03/AC/5D/03AC5D37685CFFEEFE045329BBBEFCC7.xml b/data/03/AC/5D/03AC5D37685CFFEEFE045329BBBEFCC7.xml new file mode 100644 index 00000000000..18f5b6623bf --- /dev/null +++ b/data/03/AC/5D/03AC5D37685CFFEEFE045329BBBEFCC7.xml @@ -0,0 +1,602 @@ + + + +Neodiplopeltula gen. nov. from the west coast of Sweden and reappraisal of the genus Diplopeltula Gerlach, 1950 (Nematoda, Diplopeltidae) + + + +Author + +Holovachov, Oleksandr +89D30ED8-CFD2-42EF-B962-30A13F97D203 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +oleksandr.holovachov@nrm.se + + + +Author + +Boström, Sven +528300CC-D0F0-4097-9631-6C5F75922799 +Department of Zoology, Swedish Museum of Natural History, Box 50007, SE- 104 05 Stockholm, Sweden. +sven.bostrom@nrm.se + +text + + +European Journal of Taxonomy + + +2018 + +2018-08-29 + + +458 + + +1 +34 + + + +journal article +22251 +10.5852/ejt.2018.458 +4a6349ea-1928-45b5-855f-bd13b697bb49 +3826987 +16C565AB-22A6-496F-AE95-0A876066F958 + + + + + +Neodiplopeltula onusta +( +Wieser, 1956 +) + +gen. et comb. nov. +Figs 8–10 +; +Table 4 + + + + +Diagnosis +(based on combined data) + + + +Neodiplopeltula onusta + +gen. et comb. nov. +is characterised by a +0.99–1.48 mm +long body; refractive plates underlying cephalic cuticle around amphids present and joined, +32.5–48 µm +long and +18.5–24 µm +wide; cephalic setae +3–6.5 µm +long; amphidial fovea +32–44 µm +long and +5–8 µm +wide; oral opening 2–5 from anterior end, at level with cephalic setae bases; secretory-excretory pore located opposite to posterior part of pharynx; tail 1.4–2.3 anal body diameters long; vagina with epiptygmata; spicules +52–77 µm +long. + + + + +Fig. 8. + +Neodiplopeltula onusta +( +Wieser, 1956 +) + +gen. et comb. nov. +(SMNH-169282). +A +. Male anterior end, surface view. +B +. Female anterior end, surface view. +C +. Female pharyngeal region, optical median section. +D +. Anterior part of female reproductive system. +E +. Male tail. +F +. Female tail. Scale bar = 20 µm. + + + + +Table 4 +(continued on next page). Measurements (in µm) of + +Neodiplopeltula onusta +( +Wieser, 1956 +) + +gen. et comb. nov. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + + +data for + +Diplopeltula tchesunovi + + + +recent specimens +
♀4 ♀♀3 ♂♂4 ♀♀3 ♂♂
Body length9901348–14811003–13231064 ± 104 (947–1199)1056 ± 50 (999–1094)
Body diameter (BD)?65–8848–5847 ± 9 (38–55)46 ± 7 (39–53)
Pharynx length?201–223172–212170 ± 17 (149–187)174 ± 15 (159–189)
Tail length?75–7765–8158 ± 7 (51–68)74 ± 12 (65–88)
Anal body diameter (ABD)?44–5237–4134 ± 3 (32–37)37 ± 3 (34–39)
a2617–2321–2323.6 ± 5.9 (19.3–31.9)23.4 ± 4.4 (20.5–28.4)
b5.16.3–7.35.9–6.36.3 ± 0.8 (5.3–7.2)6.1 ± 0.3 (5.8–6.3)
c14.217.5–19.212.5–17.418.7 ± 3.1 (15.2–21.4)14.6 ± 2.8 (11.4–16.9)
c’1.7??1.7 ± 0.3 (1.4–2.2)2.0 ± 0.2 (1.8–2.3)
V(%) / T(%)65.465.1–67.6?66.1 ± 1.5 (64.7–68.0)41.0 ± 7.9 (35.4–46.6)
Labial region diameter1512–16.514–1511.2 ± 1.7 (9.0–13.0)12.0 ± 0.8 (11.0–12.5)
Cephalic setae length6.53.5–5.43.5–4.54.0 ± 1.2 (3.0–5.5)3.9 ± 0.8 (3.5–5.0)
Subdorsal cephalic setae from anterior end8??6.4 ± 0.7 (5.5–7.0)7.4 ± 1.1 (6.0–8.0)
Subventral cephalic setae from anterior end???5.9 ± 0.7 (5.0–6.0)6.2 ± 0.7 (5.5–7.0)
Cephalic setae length / labial region diam.???0.4 ± 0.1 (0.3–0.5)0.3 ± 0.1 (0.3–0.4)
Anterior end of shield from anterior end???9.2 ± 0.4 (9.0–10.0)10.1 ± 0.4 (10.0–10.5)
Refractive plate length?45–4841–4736.1 ± 3.2 (32.5–38.0)35.0 ± 1.1 (34.0–36.0)
Refractive plate width?20–2419–2021.6 ± 1.5 (19.0–23.0)20.0 ± 1.4 (18.5–21.5)
Anterior end of amphid from anterior end?4–75–75.9 ± 0.9 (5.0–7.0)7.4 ± 0.8 (7.0–8.0)
Dorsal amphid branch length4041–4436–4336.2 ± 2.6 (32.5–38.0)35.9 ± 0.7 (35.0–36.5)
Ventral amphid branch length4039–4334–4135.0 ± 2.5 (32.0–37.0)34.3 ± 1.1 (33.0–35.0)
Amphid width85–75–66.6 ± 0.9 (5.5–7.5)6.7 ± 0.4 (6.0–7.0)
+ + + +Table 4 +(continued). + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+original description + + +data for + +Diplopeltula tchesunovi + + + +recent specimens +
Amphid length / width???5.6 ± 0.7 (5.0–6.6)5.4 ± 0.2 (5.2–5.7)
Anterior edge of stoma from anterior end?552.8 ± 0.8 (2.0–3.5)3.7 ± 1.1 (3.0–5.0)
Stoma length???13.6 ± 0.7 (13.0–14.5)13.6 ± 0.4 (13.0–14.0)
Stoma width???4.8 ± 0.6 (4.0–5.5)5.0
Nerve ring from anterior end?96–10690–10392 ± 6 (84–96)95 ± 6 (91–102)
Nerve ring from anterior end (% pharynx)???54.4 ± 2.9 (50.5–56.8)54.3 ± 2.6 (52.0–57.1)
Secretory-excretory pore from ant. end115110–12585–87104 ± 8 (95–112)107 ± 4 (103–112)
Secr.-excr. pore from ant. end (% pharynx)66??61.1 ± 2.9 (57.0–63.5)61.7 ± 2.9 (59.3–64.8)
Vagina or spicules length?35–4062–7219.7 ± 2.7 (16.5–23.0)63.7 ± 11.8 (56.5–77.0)
Vagina / BD or spicules / ABD??1.3–1.50.4 ± 0.1 (0.3–0.6)1.7 ± 0.3 (1.5–2.0)
Rectum or gubernaculum length??15–2024.1 ± 3.6 (21.0–28.0)10.0 ± 0.5 (10.0–10.5)
Rectum / ABD or apophysis length???0.7 ± 0.1 (0.6–0.9)16.1 ± 1.1 (15.0–17.0)
Caudal gland openings from tail terminus???terminalterminal
+ + + + +Material examined + + + + +SWEDEN +: +1 ♀ +, Skagerrak, 58°19′15.6″–20.9″ N, 10°29′33.5″–34.0″ E, soft bottom at a deep of +352–374 m +, +10 Sep. 2012 +, “Inventering Bratten” leg. (SMNH-169284); + + +1 ♂, Skagerrak, 58°23′00.8″– 22′00.8″ N, 10°20′28.8″–38.3″ E, soft bottom at a deep of +390–428 m +, +10 Sep. 2012 +, “Inventering Bratten” leg. (SMNH-169280); + + +1 ♂, Skagerrak, 58°28′21.2″–19.2″ N, 10°29′35.6″–43.6″ E, soft bottom at a deep of +248–316 m +, +11 Sep. 2012 +, “Inventering Bratten” leg. (SMNH-169281); + + +3 ♀♀ +, 1 ♂, Skagerrak, 58°27′36.7″–43.3″ N, 10°32′52.0″–59.4″ E, soft bottom at a deep of +232–240 m +, +12 Sep. 2012 +, “Inventering Bratten” leg. (SMNH-169282 – SMNH-169283). + + + + + + +Description + + + +Adult + + +Body cylindrical, posteriorly tapering in tail region, straight or weakly ventrally curved upon fixation. Cuticle finely transversely striated along entire body, except for visually smooth labial region and terminal part of tail (striation can be observed under LM but very fine and shallow and cannot be measured with confidence); longitudinal striation not observed.Somatic sensilla visible along pharyngeal region (cervical setae, see below) and on tail. Labial region bluntly rounded, lips fused. Refractive plates underlying cephalic cuticle around amphids present (periamphideal), extending from level of anteriormost edge of amphid posteriorly some distance behind posteriormost edge of amphid; refractive plates connected with each other on ventral and on dorsal sides. Inner labial sensilla indistinct. Outer labial sensilla papilliform, located on anterior surface of lips. Cephalic sensilla setiform, bases of dorsosublateral setae located at level with oral opening. Cervical sensilla papilliform, arranged in four sublateral rows at level with amphid, one or two per row. Amphids similar in shape and size between sexes: amphidial fovea inverted U-shape with dorsal branch 1.0–2.0 µm longer than ventral branch. Oral opening shifted towards dorsal side of body. Stoma subcylindrical: cheilostom broad; gymnostom barrel-shaped, with weakly cuticularised walls; stegostom short conoid, its lining uniform with lining of pharynx. Pharyngeal tubes absent. Pharynx subcylindrical, muscular, with evenly distributed myofilaments, gradually expanding towards posterior end; not subdivided in distinct sections; pharyngeal lumen uniform in thickness along entire pharynx length; valves absent. Secretory-excretory system present; secretory-excretory pore located along ventral body line opposite to 3/5 +th +of pharynx; secretory-excretory duct very short, leading from pore to ampulla; renette cell small, its body adjacent and ventral to posterior part of pharynx. Tail conoid with bluntly rounded terminus. Caudal glands opening via three separate subterminal openings, spinneret absent. + + + +Fig. 9. + +Neodiplopeltula onusta +( +Wieser, 1956 +) + +gen. et comb. nov. +(SMNH-169282). +A +. ♀, entire specimen. +B +. ♂, entire specimen. +C +. Testes. Scale bars: A–B = 100 µm; C = 50 µm. + + + +Female + + +Reproductive system didelphic, amphidelphic, ovary branches outstretched and symmetrical, on opposite sides of intestine. Anterior genital tube +109–180 µm +long, situated to either right (n = 3) or left (n = 1) of intestine; posterior genital tube +109–145 µm +long, situated to either left (n = 3) or right (n = 1) of intestine. Vulva transverse slit, located posterior to midbody. Vagina straight, with developed sphincter muscle surrounding proximal part and distinct epiptygmata in distal part; +pars refringens vaginae +absent. Sack-like spermatheca present, filled with oval spermatozoa in fertilized specimens. Rectum short. + + + +Fig. 10. + +Neodiplopeltula onusta +( +Wieser, 1956 +) + +gen. et comb. nov. +(SMNH-169282). +A +. Male anterior end, lateral view. +B +. Male stoma, lateral view. +C +. Female stoma, lateral view. +D +. Male anterior end, dorsal view. +E–G +. Variability in the shape of spicules and gubernaculum in different specimens. Scale bars = 10 µm. + + + +Male + + +Reproductive system diorchic, testes opposed; anterior testis outstretched and posterior testis reflexed (poorly discernible in most specimens due to highly granular and dark content of overlapping intestine and cannot be measured). Spicules paired and symmetrical, strongly curved, with elongated ovoid manubrium and subcylindrical shaft. Gubernaculum plate-like, with pair of strong closely set caudal apophyses variable in shape ( +Fig. 10 +E–G). Caudal setae present, several ventrosublateral and dorsosublateral pairs arranged in rows along entire tail length. + + + + + +Remarks + + + +The single female used by +Wieser (1956) +to describe this species has a slightly broader labial region and longer cephalic setae than the population from +Sweden +, but the specimens described under the name + +Diplopeltula tchesunovi + +fill this gap. The position of the oral opening is at level with the cephalic setae bases and the shape of the refractive plates underlying the cephalic cuticle around the amphids (joined along the dorsal and ventral body sides) in both +type +female and recent specimens are identical and confirm the conspecificity. + + + + \ No newline at end of file