diff --git a/data/30/ED/65/30ED651F014B52878F4E5ABCF5069BE4.xml b/data/30/ED/65/30ED651F014B52878F4E5ABCF5069BE4.xml new file mode 100644 index 00000000000..4b709451002 --- /dev/null +++ b/data/30/ED/65/30ED651F014B52878F4E5ABCF5069BE4.xml @@ -0,0 +1,1203 @@ + + + +New species and a fascinating diversity of Chironomidae (Diptera, Insecta) in and around an overlooked urban vernal pool + + + +Author + +Namayandeh, Armin +0000-0003-2136-0497 +Department of Environmental and Life Sciences, Trent University, 1600 West Bank Drive, Peterborough, ON, Canada & Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Guerra, Sergio +https://orcid.org/0009-0009-7614-5314 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Islam, Natasha +https://orcid.org/0009-0009-8270-4004 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +James, Taylor +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Hudson, Patrick L. +0000-0002-7646-443X +5597 New Meadow Drive, Ypsilanti, MI 48197, USA + + + +Author + +Ghaderi, Edris +0000-0002-9875-7735 +Department of Fisheries Sciences, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran & Department of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran + + + +Author + +Yusuf, Thameena +https://orcid.org/0009-0000-6296-6693 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Vasquez, Adrian A. +0000-0002-2434-3388 +Department of Biology, College of Liberal Arts and Sciences, Mercer University, 1501 Mercer University Drive, Macon, GA 31207, USA + + + +Author + +Ram, Jeffrey L. +0000-0002-1063-546X +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + +text + + +ZooKeys + + +2024 + +2024-07-29 + + +1208 + + +133 +163 + + + +journal article +10.3897/zookeys.1208.124495 +FFB06B11-084A-4C08-A050-AFBA7655DF2F + + + + + +Rheocricotopus (s. s.) angustus +Namayandeh & Hudson + +sp. nov. + + + + +Figs 10 +, +11 +, +12 + + + + +Type material. + + + + +Holotype + +1 male +; +Canada +, +Newfoundland +, +Terra Nova National Park +; +Blue Hill Road +, + +48.598 ° N +, - +53.9702 ° W + +; leg. +E. Perry +; + +21. v. 2013 + +, dep. + +CBG + + +. + +Paratypes +2 females +, +CANADA +, +Yukon Territory +, +Ivvavik National Park +, + +69.169 ° N +, - +140.167 ° W + +; leg. +N. Perry +; + +29. vi. 2014 + +, dep. + +CBG + + +. + + +Paratype + +1 larva +; +USA +, +Michigan +, +Detroit +, +Palmer Park, Pond A +; + +42.42766 ° N +, +83.11741 ° W + +; leg. +P. L. Hudson +; + +30. vi. 2022 + +, dep. + +ARC + + +. + + + + +Additional material examined. + + + +Rheocricotopus +( +s. s. +) +effusus +(Walker, 1856) + +, +1 male +, associated pupa and larval exuviae; +USA +, +South Dakota +, Yankton, Ed’s Creek, Gavins Point National Fish Hatchery, leg. P. L. Hudson, det. O. A. Sæther, dep. +PLH +. + +Rheocricotopus + +( +s. s. +) + +effusus +(Walker, 1856) + +, +1 male +, associated pupa and larva exuviae; +USA +, +South Dakota +, Yankton, Ed’s Creek, Gavins Point National Fish Hatchery, +11. x. 1971 +, leg. P. L. Hudson, dep. +PLH +. + +Rheocricotopus + +( +s. s. +) + +effusoides +Sæther, 1985 + +, +1 male +, associated pupa and larva exuviae; +USA +, +South Dakota +, Yankton, Marne Creek, +12. iii. 1972 +, leg. P. L. Hudson, dep. +PLH +. + +Rheocricotopus + +( +s. s. +) + +unidentatus +Sæther & Schnell, 1988 + +, +1 larva +. +GERMANY +, Federal State +Hessen +, Freiensteinau, Nature Park Vogelsberg, (north-east f. Frankfurt), forest spring, +02. iv. 2017 +, leg. +T +. Bendt, dep. +TB +. + +Rheocricotopus + +( +s. s. +) + +pauciseta +Sæther, 1969 + +, +holotype +, +1 male +, associated pupa and larval exuviae; +CANADA +, +British Columbia +, Marion Lake, University of +British Columbia +Forestry Farm, Haney, small mountain stream, +15. vii. 1967 +, leg., A. L. Hamilton and O. A. Sæther, dep. + +CNC + +, No. 9990. + + + + +Diagnostic characters. + + + +R. angustus + +sp. nov. +can be separated from other + +Rheocricotopus + +by the combination of the following characteristics: Adults with elongate ellipsoid humeral pits, without smaller basal pit, narrowing at the base for the male. Adult male with AR 1.4, anal point very short with 6 lateral setae, superior volsella with caudomedian projection strongly bent, thick, short and triangular. Adult female with AR 0.29, costa extension 115–119 µm long, notum 207–228 µm long. Fourth instar larva with AR 2.2, SI bifid with equal branches, SII long and thin, 37 μm long, mentum’s cardinal beard with 27 setae, seta submenti very long. + + + + +Description. + + +Male +( +n += 1). Total length +3.3 mm +. Wing +1.8 mm +long and +0.55 mm +wide. + + + +Coloration +. + +Head, thorax, halters, legs, tergites, sternites, and hypopygium blackish brown. Wings pale brown. + + +Head +(Fig. +10 A, B +). Antenna with 13 flagellomeres, last flagellomere with 14 sensilla chaetica, groove starts at 4 +th +flagellomere (Fig. +10 A +), AR 1.4 ( +n += 1). Eyes hairy, without dorsomedial extension. Temporal setae 5, including 2 frontals, 2 postoculars and 1 outer vertical. Tentorium 178 μm long (Fig. +10 B +). Clypeus rectangular, 89 μm long and 136 μm wide, bearing 18 setae, setae 56–65, 60 μm long. Palpal segment lengths (in μm): 97, 69, 122, 135, 190. Third palpomere with single thin sensilla clavata. + + + + + + + +Rheocricotopus +( +s. s. +) +angustus + +sp. nov. +, adult male +A +antenna +B +head +C +thorax +D +wing +E +hypopygium. Scale bars: 100 µm. + + + +Thorax +(Fig. +10 C +). Acrostichals 12, start close to antepronotum; dorsocentrals 19–20; prealars 5; scutellars +12 in +single row; antepronotals 7. Humeral pit elongate ellipsoid narrowing at the base, about half the size of antepronotum, and no smaller basal pit. + + +Wing +(Fig. +10 D +). Brachiolum with 1 seta. Squama with 13 setae. R with 10 setae; all other veins bare. Costa extension is 44 μm long. Anal lobe developed. + + + +Legs +. + +Fore tibia spur and tarsal segments missing, mid tibia spurs 26 and 22 μm long, hind tibia spurs 55 and 17 μm long, hind tibia comb with 13 spines. Lengths and proportions of legs as in Table +5 +. + + + + + + +Male leg lengths (μm) and proportions of + +Rheocricotopus +( +s. s. +) +angustus + +sp. nov. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
-feti +ta +1 + +ta +2 + +ta +3 + +ta +4 + +ta +5 +LRBVSV
+ +P +1 + +824857––––––––
+ +P +2 + +767782375219144951040.503.44.1
+ +P +3 + +7399125162912291211160.602.93.2
+ + +Hypopygium +(Fig. +10 E +). Laterosternite IX 62 μm long, with 12 setae. Anal point very short, 19 μm long and 14 μm wide at the base, triangular with apex pointed and with 6 lateral setae. Transverse sternapodeme with well-developed oral projections; sternapodeme 135 μm long. Phallapodeme 48 μm long. Superior volsella with caudomedian projection tick, short and triangular, parallel but not touching in middle, strongly bent, 35 μm long. Inferior volsella triangular lobe covered in numerous simple setae, apex slightly bent. Gonocoxite 208 μm long. Gonostylus 98 μm long; crista + +dorsalis + +very large covering more than half of gonostylus, mega setae 12 μm long. HR 2.1, HV 3.4. + + +Female +( +n += 2). Total length 1.8–2.5, +2.2 mm +. Wing 1.5–1.9, +1.7 mm +long and 0.56–0.61, +0.58 mm +wide. + + + +Coloration +. + +Same as the male. + + +Head +(Fig. +11 A +). Antenna with 5 flagellomeres, last flagellomere with 6 sensilla chaetica, Antennal segments 1–5 (in μm): 71–101, 86; 42–55, 48; 39–48, 44; 62; 78; AR 0.29. Eyes hairy, reniform. Temporal setae 5 including 1 frontal, 2 outer verticals, and 2 postoculars. Tentorium 144–174, 159 μm long. Clypeus rectangular, 65–82, 74 μm long and 96–110, 104 μm wide, bearing 12 setae, setae 53–63, 59 μm long. Palpal segment lengths (in μm): 27–31, 29; 31–34, 32; 57–71, 64; 61; 72–90, 81. + + + + + + + +Rheocricotopus +( +s. s. +) +angustus + +sp. nov. +, adult female +A +head +B +thorax +C +wing +D +genitalia ventral +E +genitalia dorsal. Scale bars: 100 µm. + + + +Thorax +(Fig. +11 B +). Acrostichals 21; dorsocentrals 12–18, 15, in a single row; prealars 3; scutellars +6 in +single row; antepronotals 5. The humeral pit is similar to that of the male but not narrow at the base. + + +Wing +(Fig. +11 C +). Brachiolum with 1 seta. Squama with 10 setae. R with 10 setae; R +1 +with 5–8, 7 setae; R +4 + 5 +9–14, 12 setae; other veins without setae. Costa extension 115–119, 117 μm. + + + +Legs +. + +Fore tibia spur missing, mid tibia spurs 17 and 21 μm long, hind tibia spurs 17 and 36 μm long; hind tibia comb with around 13 spines. The lengths and proportions of the legs are shown in Table +6 +. + + + + + + +Female leg lengths (μm) and proportions of + +Rheocricotopus +( +s. s. +) +angustus + +sp. nov. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
-feti +ta +1 + +ta +2 + +ta +3 + +ta +4 + +ta +5 +LRBVSV
+ +P +1 + +491513––––––––
+ +P +2 + +5765222131309859750.413.65.2
+ +P +3 + +610666––––––––
+ + +Genitalia +(Fig. +11 D, E +). Seminal capsules ovoid, 82–119, 102 μm long, and 56–80, 66 μm wide; spermathecal ducts without loop, with well-developed bulb. Notum 207–228, 218 µm long, notum and ramus 256–285, 270 µm long. Gonapophysis VIII divided into large ventrolateral covering smaller dorsomesal lobe. Apodeme lobe distinct. Gonocoxite developed, with around 8 setae (Fig. +11 E +). Tergite IX divided in two pale ovoid sections each bearing around 8–9 setae. Cercus large, base semi-circular, apex pediform, 66–89, 78 µm long, and 71–96, 83 µm wide. + + +Immatures. +The pupa is unknown. The larva is associated by molecular DNA-barcoding. + + +4 +th +instar larva ( +n += 1). Total length +7.1 mm +. Head 354 μm long and 381 μm wide. + + + +Coloration of the mounted specimen +. + +Head capsule yellowish brown with occipital region darker than rest of the head capsule, body greyish brown. + + +Head +(Fig. +12 A – D +). Antenna 5 segmented; segments length in μm: 86, 14, 10, 7, 7; AR 2.2; basal antennal segment 20 μm wide, distance from the ring organ to base of basal segment 8 μm. Lauterborn organ robust covering the 3 +rd +segment, blade damaged. Labral SI bifid with equal branch, SII – +SIII +simple, SII long and thin (Fig. +12 B +). Premandible simple, 78 μm long (Fig. +12 B +). Mandible much paler in basal half, apical tooth shorter than combined width of three inner teeth; seta subdentalis reaches the base of first inner tooth; setae interna with several long branches (Fig. +12 C +), mandible 137 μm long. Mentum dark, with large median tooth and 5 pairs of lateral teeth, median tooth slightly notched (possibly worn off), median tooth 35 μm wide, 3.6 × the 1 +st +lateral teeth; seta submenti very long, upright reaching the base of median tooth, base aligned with the 4 +th +inner teeth (Fig. +12 D +); mentum 92 μm long and 134 μm wide; ventromental plate large, extended beyond the mentum, 116 μm long, and 29 μm wide, cardinal beard with 27 lateral setae. Postmentum 242 μm long. + + + + + + + +Rheocricotopus +( +s. s. +) +angustus + +sp. nov. +, fourth instar larva +A +antenna +B +labrum and premandible +C +mandible +D +mentum +E +posterior portion of the larva +F +procercus, arrows indicate the tubercles. Scale bars: 50 µm. + + + +Abdomen +(Fig. +12 E, F +). Posterior parapods 301 μm long and 208 μm long, bearing around 15 simple claws. Procercus 34 μm long and 18 μm wide, bearing two small basal spurs and 4 apical setae (Fig. +12 F +), apical setae 447 μm long, supraanal setae 49 μm long. 4 anal tubules present, 78–92 μm long. + + + + +Etymology. + + +The new species is named after the city of Detroit. The name of the city comes from the French word +détroit +meaning strait or river, which translates to + +angustus + +in Latin. + + + + +Distribution. + + +Canada +( +Manitoba +, +New Brunswick +, Newfoundland, +Nunavut +, +Ontario +, +Yukon Territory +); +USA +( +Michigan +). + + + + +Remarks. + + +Based on the shape of superior volsella, + +R. angustus + +sp. nov. +belongs to the + +effusus + +group. A detailed examination and comparison of the species in this group is provided by +Namayandeh and Beresford (2018) +. Here, we mentioned that the elongate ellipsoidal shape of the humeral pit of + +R. angustus + +sp. nov. +adults are quite distinguishable from other known species in this group, except + +Rheocricotopus + +( +s. s. +) + +unidentatus +Sæther & Schnell, 1988 + +. However, in + +R. angustus + +sp. nov. +, no additional smaller basal pit is present. Another distinguishing characteristic of + +R. angustus + +sp. nov. +male is the very short anal point of the male with few lateral setae. The female of + +R. angustus + +sp. nov. +can be separated from the known females of this group by a lower antennal ratio and longer notum and costa extension. + + +The key to adult males of the + +effusus + +group we provided in this study is based on a previous key by +Namayandeh and Beresford (2018) +, which was based on a key by +Sæther (1985) +. The provided key can only partially separate the species in this group. + +Rheocricotopus + +( +s. s. +) + +effusoides +Sæther, 1985 + +and + +Rheocricotopus + +( +s. s. +) + +effusus +(Walker, 1856) + +can be separated based on the size and ratio in all life stages ( +Sæther 1985 +). + +R. unidentatus + +is quite distinguishable from other species based on the larval mentum characteristic. + + +The larva of + +R. angustus + +sp. nov. +can be separated from other known larvae in this group by a higher antennal ratio, a bifid SI with equal branches, and SII 37 μm long. Except for + +R. unidentatus + +, which has a distinguishing single median mental tooth, there are overlapping characteristics of larvae in this group, which makes their separation difficult. These include but are not limited to the length of the head, postmentum, and basal antennal segment, and number cardinal beard setae (Table +7 +). However, + +R. effusus + +and + +Rheocricotopus pauciseta +Sæther, 1969 + +are in the lower range of the basal antennal length in comparison to other known larvae of this group. + +R. unidentatus + +and + +R. effusoides + +are in the higher range of cardinal beard setae than other species in this group, and + +R. angustus + +sp. nov. +and + +R. pauciseta + +are in the lower range. We found that the length and shape of labral SII could also distinguish the larvae in this group (see Table +7 +). We examined the larval procercus of all species in this group, except for the voucher specimen of + +R. effusoides + +, in which the abdomen was missing, and consulted Mr. Thomas Bendt on the larva of + +R. unidentatus + +from +Germany +, which confirms that all larvae possess a pair of small procercal spurs. However, the shape and size of these spurs are very similar (Table +7 +). + + + + + + +Comparison of some relevant larval characteristics of + +Rheocricotopus effusus + +group. A +1 += Antennal segment 1, HL = Head length, L = Length, No. = Number, TL = Total length, W = Width; for other abbreviations, see +Sæther (1980) +. All measurements are in µm unless otherwise indicated. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
- + +R. angustus + +sp. nov. + +R. effusus + +R. effusoides + +R. pauciseta +R. unidentatus
+TL (mm) +7.14–5.25.2–4.0 – 7.2
+HL +354430424–514400450–600
+AR +2.21.5–1.81.8–2.11.551.5–2.0
+ +A +1 +L + +8645–6472–8562–6469–87
+ +Basal A +1 +W + +20–19 – 2113–1815–24
+Distance from base to RO +8512–156–108–15
+SI +Bifid, even branchesBifid, uneven branchesBifid, uneven branchesBifid *Bifid, uneven branches
+SII L +3714–22534019–22
+Mentum median tooth +BifidBifidBifidBifidSingle
+No. of cardinal beard setae +2725–3132–332028–40
+Postmentum L +242218–226233–259234223–249
+Procercus L +3426–2841–454030–38
+Procercus spurs +PresentPresent–PresentPresent
+No. procercus apical setae +44–5–55–6
+Apical setae L +447160549–567354450–563
+ + +* The labral SI of the examined larvae appears bifid. However, the length of the branches is hard to detect due to the condition of the mount. + + + + \ No newline at end of file diff --git a/data/CB/D6/78/CBD6788BAF8F52AC8D05EFDEC3D61F79.xml b/data/CB/D6/78/CBD6788BAF8F52AC8D05EFDEC3D61F79.xml new file mode 100644 index 00000000000..25719f1bb03 --- /dev/null +++ b/data/CB/D6/78/CBD6788BAF8F52AC8D05EFDEC3D61F79.xml @@ -0,0 +1,416 @@ + + + +New species and a fascinating diversity of Chironomidae (Diptera, Insecta) in and around an overlooked urban vernal pool + + + +Author + +Namayandeh, Armin +0000-0003-2136-0497 +Department of Environmental and Life Sciences, Trent University, 1600 West Bank Drive, Peterborough, ON, Canada & Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Guerra, Sergio +https://orcid.org/0009-0009-7614-5314 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Islam, Natasha +https://orcid.org/0009-0009-8270-4004 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +James, Taylor +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Hudson, Patrick L. +0000-0002-7646-443X +5597 New Meadow Drive, Ypsilanti, MI 48197, USA + + + +Author + +Ghaderi, Edris +0000-0002-9875-7735 +Department of Fisheries Sciences, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran & Department of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran + + + +Author + +Yusuf, Thameena +https://orcid.org/0009-0000-6296-6693 +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + + + +Author + +Vasquez, Adrian A. +0000-0002-2434-3388 +Department of Biology, College of Liberal Arts and Sciences, Mercer University, 1501 Mercer University Drive, Macon, GA 31207, USA + + + +Author + +Ram, Jeffrey L. +0000-0002-1063-546X +Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA + +text + + +ZooKeys + + +2024 + +2024-07-29 + + +1208 + + +133 +163 + + + +journal article +10.3897/zookeys.1208.124495 +FFB06B11-084A-4C08-A050-AFBA7655DF2F + + + + + +Chironomus +nr. sp. +parariparius +of +Martin (2023) + + + + + +Figs 13 A – E + + + + +Material examined. + + + +1 male +; +USA +, +Michigan +, +Detroit +, +Palmer Park, Pond A +; + +42.42766 ° N +, +83.11741 ° W + +; leg. +P. L. Hudson +; + +30. vi. 2022 + +, dep. + +ARC + + +. + + + + +Diagnostic characters. + + +The adult male of +C. +nr. sp. +parariparius +can be separated from other + +Chironomus +species + +by the combination of the following characteristics: AR 3.1; frontal tubercle present, 12 μm long; wing without any pattern; fore tibia scale 60 μm long; tergite IX with 9 median setae on two adjacent pale patches; superior volsella S-type, with robust apex and base with 5 long setae. + + + + +Description. + + +Male +( +n += 1). Total length 7.0 mm. Wing +3.6 mm +long and +0.90 mm +wide. + + + +Coloration of the mounted specimen +. + +Head brown. Thorax brown, with scutellum and humeral region pale yellowish. Abdominal tergites with posterior 2 / 3 +rd +brown and the anterior 1 / 3 +rd +pale yellowish (Fig. +13 A +). Halter and wing pale brown. + + + + + + + +Chironomus +nr. sp. +parariparius +of +Martin (2023) + +, adult male +A +abdominal tergites III – V +B +antenna +C +head +D +wing +E +hypopygium. Scale bars: 100 µm. + + + +Head +(Fig. +13 B, C +). Antenna with 11 flagellomeres, the last flagellomere with 4 sensilla chaetica (Fig. +13 B +), each of 2 +nd +– 4 +th +antennal flagellomere with a pair of long sensilla chaetica; groove starts at 4 +th +flagellomere, AR 3.1. Eyes bare, with well-extended dorsomedial extension. Frontal tubercle present, 12 μm long. Temporal setae 25, uniserial. Tentorium 201 μm long. Clypeus nearly squared, 130 μm long and 146 μm wide, bearing 36 setae, setae 108 μm long. Palpal segment lengths (in μm): 216, 116, 272, 234, 337. Third palpomere with 7 sensilla clavata. + + + +Thorax +. + +Acrostichals 2, close to antepronotum; dorsocentrals +14 in +single row; prealars 5; scutellars +14 in +double rows; antepronotals 1. Mid-scutum hump is present. + + +Wing +(Fig. +13 D +). Wing without any pattern. Brachiolum with 1 seta, squama with 17 setae, R with 40 setae, R +1 +with 47 setae, R +4 + 5 +with 65 setae. Anal lobe developed. + + + +Legs +. + +Mid and hind legs missing. Fore tibia scale 60 μm long. The lengths and proportions of the legs as in Table +8 +. + + + + + + +Male leg lengths (μm) and proportions of + +Chironomus +nr. sp. +parariparius +by +Martin, 2023 + +. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
-feti +ta +1 + +ta +2 + +ta +3 + +ta +4 + +ta +5 +LRBVSV
+ +P +1 + +15521328214412169037223381.61.61.3
+ +P +2 + +––––––––––
+ +P +3 + +––––––––––
+ + +Hypopygium +(Fig. +13 E +). Tergite IX with 12 median setae, seven on adjacent small pale patches and 5 more posteriorly without pale patches, all pointing toward anal point. Anal point broad, parallel-sided with a round apex, 119 μm long. Sternapodeme squared, 129 μm long. Phallapodeme 202 μm long. Superior volsella S-type with robust apex, and base with 5 long setae, 83 μm long. Inferior volsella 178 μm long, base slightly bent, apex reaches the mid-section of the anal point. Gonocoxite 111 μm long. Gonostylus 204 μm long, with 5 long setae at apex; HR 0.5, HV 2.6. + + + + +Remarks. + + +The single adult male of the + +Chironomus +species + +we collected from Pond A closely resembles the + +Chironomus +species + +Martin (2023) +described as Species 2 c. or + +Chironomus +species + +parariparius +. According to +Martin (2023) +, only the images of the adult male are available based on the reared collection of specimens by J. E. Sublette, now kept in the Zoological Museum of the University of +Minnesota +, St. Paul. Pupa is only described by the caudolateral spur of segment VIII; however, larva morphology and cytology are described by +Martin (2023) +in detail. +Martin (2023) +describes the species’ habitat as snow pools, similar to Pond A. The adult male from pond A is quite distinguishable from other species in the + +C. riparius + +group based on the characteristics of the hypopygium (Jon Martin, pers. comm. +18 March 2024 +). + + + + \ No newline at end of file