<documentid="F5F428780F1A323D99124551E75F0B00"ID-DOI="10.11646/phytotaxa.509.1.6"ID-ISSN="1179-3163"ID-Zenodo-Dep="5426040"IM.materialsCitations_approvedBy="felipe"IM.metadata_approvedBy="felipe"IM.tables_requiresApprovalFor="existingObjects,plazi"IM.taxonomicNames_approvedBy="carolina"checkinTime="1630396302215"checkinUser="marcus"docAuthor="García-Jiménez, Jesús, Ayala-Vásquez, Olivia, Guevara-Guerrero, Gonzalo, Garza-Ocañas, Fortunato & Fuente, Javier Isaac De La"docDate="2021"docId="03BC8D23FFACFF8886A9FF11FB57FC47"docLanguage="en"docName="phytotaxa.509.1.6.pdf"docOrigin="Phytotaxa 509 (1)"docSource="http://dx.doi.org/10.11646/phytotaxa.509.1.6"docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.7:Phytotaxa.2014-.journal_article"docStyleId="96748F8F1B6C902996E134952A3A36B9"docStyleName="Phytotaxa.2014-.journal_article"docStyleVersion="7"docTitle="Tuber mixtecorum J. Garcia, Ayala-Vasquez & de la Fuente 2021, sp. nov."docType="treatment"docVersion="4"lastPageNumber="118"masterDocId="FF85F55BFFAFFF8D8621FFE5FF8FFFB3"masterDocTitle="Tuber mixtecorum (Tuberaceae, Pezizales) a new truffle in the Maculatum clade from Mexico"masterLastPageNumber="120"masterPageNumber="113"pageNumber="116"updateTime="1719947619869"updateUser="carolina"zenodo-license-figures="UNSPECIFIED"zenodo-license-treatments="UNSPECIFIED">
<mods:titleid="8D0A4DEF58D90A63CEC76D223743EE25">Tuber mixtecorum (Tuberaceae, Pezizales) a new truffle in the Maculatum clade from Mexico</mods:title>
<mods:affiliationid="3B006190EA53DA699798C9984C5955C2">Tecnológico Nacional de México. Instituto Tecnológico de Ciudad Victoria. Blvd. Emilio Portes Gil # 1301 Pte. CP 87010, Ciudad Victoria, Tamaulipas, Mexico. & jgarjim @ yahoo. com. mx; https: // orcid. org / 0000 - 0001 - 9290 - 1460</mods:affiliation>
<mods:affiliationid="A91BDF780986A9D8E1EDA66E84096622">Tecnológico Nacional de México. Instituto Tecnológico de Ciudad Victoria. Blvd. Emilio Portes Gil # 1301 Pte. CP 87010, Ciudad Victoria, Tamaulipas, Mexico. & yootspooj @ gmail. com; https: // orcid. org / 0000 - 0002 - 8970 - 9571</mods:affiliation>
<mods:affiliationid="A58D889036F6F6A5AF75F5D9BB3422E3">Tecnológico Nacional de México. Instituto Tecnológico de Ciudad Victoria. Blvd. Emilio Portes Gil # 1301 Pte. CP 87010, Ciudad Victoria, Tamaulipas, Mexico. & guevaragg @ hotmail. com; https: // orcid. org / 0000 - 0002 - 2707 - 4531</mods:affiliation>
<mods:affiliationid="6E5F59B365076B1285FCD437C1D6B32B">Universidad de Quintana Roo, División de Ciencias de la Salud, Av. Erick Paolo Martínez S / N, Chetumal, Quintana Roo, CP 77039, Mexico. & jdelafuenteitcv @ gmail. com; https: // orcid. org / 0000 - 0003 - 4667 - 1574</mods:affiliation>
<taxonomicNameid="4C1547B6FFACFF8E86A9FF11FCA0FEBC"ID-CoL="B2ZHL"authority="J. Garcia, Ayala-Vasquez & de la Fuente"authorityName="J. Garcia, Ayala-Vasquez & de la Fuente"authorityYear="2021"box="[136,815,244,271]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Fungi"order="Pezizales"pageId="3"pageNumber="116"phylum="Ascomycota"rank="species"species="mixtecorum"status="sp. nov.">
<figureCitationid="132E20B0FFACFF8E85BFFF11FC6CFEBD"box="[926,995,244,270]"captionStart="FIGURE 2"captionStartId="4.[136,229,1824,1846]"captionTargetBox="[151,1436,190,1800]"captionTargetId="figure-25@4.[151,1436,190,1800]"captionTargetPageId="4"captionText="FIGURE 2. Tuber mixtecorum (Holotype). A. Details of the gleba. B. Ascospore showing the alveolate ornamentation. C. Asci and ascospores. D–E. Details of the terminal elements of the peridium. Scale bar: 10 mm (A); 20 µm (B–E)."figureDoi="http://doi.org/10.5281/zenodo.5426044"httpUri="https://zenodo.org/record/5426044/files/figure.png"pageId="3"pageNumber="116">Fig. 2</figureCitation>
<collectingDateid="EFEFE31DFFACFF8E82E5FE81FAD1FECA"box="[1220,1374,356,377]"pageId="3"pageNumber="116"value="2018-08-19">19 August 2018</collectingDate>
<collectionCodeid="ED04A4F0FFACFF8E8740FE6DFE16FE2E"box="[353,409,392,413]"country="Mexico"httpUri="http://biocol.org/urn:lsid:biocol.org:col:13347"lsid="urn:lsid:biocol.org:col:13347"name="Instituto Tecnológico de Ciudad Victoria"pageId="3"pageNumber="116"type="Herbarium">ITCV</collectionCode>
[JGARCIA-21880-
<collectionCodeid="ED04A4F0FFACFF8E847DFE62FD19FE2E"box="[604,662,391,413]"country="Mexico"httpUri="http://biocol.org/urn:lsid:biocol.org:col:13347"lsid="urn:lsid:biocol.org:col:13347"name="Instituto Tecnológico de Ciudad Victoria"pageId="3"pageNumber="116"type="Herbarium">ITCV</collectionCode>
<taxonomicNameid="4C1547B6FFACFF8E872AFE49FE30FE71"authorityName="J. Garcia, Ayala-Vasquez & de la Fuente"authorityYear="2021"box="[267,447,428,450]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="3"pageNumber="116"phylum="Foraminifera"rank="species"species="mixtecorum">Tuber mixtecorum</taxonomicName>
clade by the whitish to brownish grey peridium, composed of interwoven or postrate cells, with erect claviform terminal elements of 19–24 µm, ellipsoid ascospores of 15–33.9 × 11.3–23.3 µm and the putative association to
. Phylogenetic analysis based on Bayesian maximum likelihood and posterior probability phylogenetic analysis generated from ITS rDNA, showing
<taxonomicNameid="4C1547B6FFACFF8E8770F8DEFD8FF8E2"authorityName="J. Garcia, Ayala-Vasquez & de la Fuente"authorityYear="2021"box="[337,512,1851,1873]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="3"pageNumber="116"phylum="Foraminifera"rank="species"species="mixtecorum">
, globular, subglobose, slightly hunched over, whitish or brownish gray in color (30A2), stains dark brown when handled, subtomentose, in some areas somewhat glabrous, dry texture, no rhizomorphs at the base.
, without changes in color when cut, marbled gleba, blackish or dark violet color (18F5), with violet white veins (18A2), some of them ending in peridium. Fungoid smell, soft. Fungoid taste, oily.
<taxonomicNameid="4C1547B6FFABFF898728F8C5FDBEF885"authority="(Holotype)"authorityName="J. Garcia, Ayala-Vasquez & de la Fuente"authorityYear="2021"baseAuthorityName="Holotype"box="[265,561,1824,1846]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="4"pageNumber="117"phylum="Foraminifera"rank="species"species="mixtecorum">
of 173–239 µm in thickness. Outer layer 52–89 µm thick, formed by interwoven tubular or prosenchymal hyphae, 3–6 µm in diameter, with some loosely interwoven with emerging cytidioid elements, claviform or subclaviform, 19–24 µm long, hyaline in KOH, immutable in Melzer, without intracellular content, thick-walled. Internal layer 114– 138 µm thick, formed by interwoven hyphae or in some areas subglobose hyphae, or rarely prostrate, 4–9 µm wide, hyaline, without intracellular content, smooth and thin walled.
subglobose to ellipsoid of 15–33.9 × 11.3–23.3 µm without ornamentation, alveoli 2–4 µm long, polygonal (4–7) isodiametric, 4–8 alveoli lengthwise, 3–5 widthwise, brown to pale brown, thick wall up to 3 µm.
—This new species is characterized from other species within the Maculatum clade by the whitish, subtomentous peridium, with short ellipsoid spores of 15.0–33.9 × 11.3–3.3 µm. It is similar to
<bibRefCitationid="EF8441C4FFAAFF88873EFD97FE76FD3F"author="Pegler, D. N. & Spooner, B. M. & Young, T. W. K."box="[287,505,626,653]"pageId="5"pageNumber="118"refId="ref4303"refString="Pegler, D. N., Spooner, B. M. & Young, T. W. K. (1993) British truffles. A revision of British hypogeous fungi. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp."type="book"year="1993">
. (1991) has longer spores (40–80 × 40–54 µm) while the European material has wider spores (25–55 × 21–40 µm). This species has the shortest spores within the northamerican species of the Maculatum group (
<tableCitationid="C697098EFFAAFF8885F3FD3BFBA4FD4B"box="[978,1067,734,760]"captionStart="TABLE 2"captionStartId="5.[136,224,1060,1084]"captionText="TABLE 2. Comparative morphology of North American species of Tuber from the Maculatum clade."pageId="5"pageNumber="118">Table 2</tableCitation>
). Another significant difference is the absence of flat or pyramidal warts present in other species of the group (Guevara-Guerrero
can also present a smooth looking peridium, however, it is easily separated from
<taxonomicNameid="4C1547B6FFAAFF8882B6FCC2FAB9FCF3"authorityName="J. Garcia, Ayala-Vasquez & de la Fuente"authorityYear="2021"box="[1175,1334,807,832]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="5"pageNumber="118"phylum="Foraminifera"rank="species"species="mixtecorum">
<taxonomicNameid="4C1547B6FFAAFF8884DDFC8AFAEEFC3B"authority="Guevara, Bonito & Trappe (2015: 370)"authorityName="Guevara, Bonito & Trappe"authorityPageNumber="370"authorityYear="2015"box="[764,1377,878,905]"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="5"pageNumber="118"phylum="Foraminifera"rank="species"species="tequilanum">
<bibRefCitationid="EF8441C4FFAAFF888580FC8BFAEEFC3B"author="Guevara, G. & Bonito, G. & Cazares, E. & Healy, R. & Vilgalys, R. & Trappe, J. M."box="[929,1377,878,905]"pageId="5"pageNumber="118"pagination="367 - 374"refId="ref3720"refString="Guevara, G., Bonito, G., Cazares, E., Healy, R., Vilgalys, R. & Trappe, J. M. (2015) Novel Tuber spp. (Tuberaceae, Pezizales) in the Puberulum group from Mexico. Ascomycete 7: 367 - 374."type="journal article"year="2015">Guevara, Bonito & Trappe (2015: 370)</bibRefCitation>
</taxonomicName>
in the whitish ascocarps, thickness and cystidioid elements of peridium but it differs in spore size being the longer in
<taxonomicNameid="4C1547B6FFAAFF8883B6FC76FE89FC63"authorityName="Guevara, Bonito & Trappe"authorityYear="2015"class="Pezizomycetes"family="Tuberaceae"genus="Tuber"kingdom="Chromista"order="Pezizales"pageId="5"pageNumber="118"phylum="Foraminifera"rank="species"species="tequilanum">
<bibRefCitationid="EF8441C4FFAAFF88858BFC3FFB43FC47"author="Gomez-Reyes, V. M. & Vasquez-Marrufo, G. & Ortega-Gomez, A. M. & Guevara-Guerrero, G."box="[938,1228,986,1013]"pageId="5"pageNumber="118"pagination="37 - 48"refId="ref3529"refString="Gomez-Reyes, V. M., Vasquez-Marrufo, G., Ortega-Gomez, A. M. & Guevara-Guerrero, G. (2018) Ascomicetos hipogeos de la region occidental del Sistema Volcanico Transversal, Mexico. Acta Botanica Mexicana 125: 37 - 48. https: // doi. org / 10.21829 / abm 125.2018.1327"type="journal article"year="2018">
