<mods:affiliationid="D015D9D58B3321F711F9196B53B9D9E3">University of Innsbruck, Research Department for Limnology, A- 5310 Mondsee, Austria</mods:affiliation>
<mods:affiliationid="751855D967693019FE575E5E22780738">University of Göttingen, Experimental Phycology and Culture Collection of Algae, D- 37073 Göttingen, Germany & M. G. Kholodny Institute of Botany, National Academy Science of Ukraine, Kyiv 01601, Ukraine</mods:affiliation>
<mods:affiliationid="28B05D84573BE21945863B506F5BF9E8">Leibniz Institute of Freshwater Ecology and Inland Fisheries, Department of Limnology of Stratified Lakes, D- 16775 Stechlin-Neuglobsow, Germany</mods:affiliation>
<taxonomicNameid="4C415CBFFFC96638FF62FDCB54A8B2C4"authority="Proschold & Darienko"authorityName="Proschold & Darienko"authorityYear="2018"box="[136,722,634,661]"class="Chlorophyceae"family="Chlamydomonadaceae"genus="Chlamydomonas"kingdom="Plantae"order="Chlamydomonadales"pageId="4"pageNumber="25"phylum="Chlorophyta"rank="species"species="schloesseri"status="sp. nov.">
<figureCitationid="137A3BB9FFC96638FCAAFDCD55F9B2C4"box="[832,899,636,661]"captionStart="FIGURE 1"captionStartId="4.[136,229,2017,2038]"captionTargetBox="[155,1432,1150,1988]"captionTargetId="figure-357@4.[155,1433,1150,1988]"captionTargetPageId="4"captionText="FIGURE 1. Morphology and phenotypic plasticity of Chlamydomonas schloesseri sp. nov. A–J. vegetative cells, arrow in F. marked the eyespot; K–O. different sporangia; scale bar = 10 μm."figureDoi="http://doi.org/10.5281/zenodo.13702994"httpUri="https://zenodo.org/record/13702994/files/figure.png"pageId="4"pageNumber="25">
: Mature vegetative cells are spherical, broadly ellipsoidal, ovoid or sometimes obovoid 8.7 x 9.3 to 9.6 x 11.9 μm in size. Cell wall is thin, without papilla, two apical contractile vacuoles. Chloroplast is cup-shaped, with thickened basal part. The surface of chloroplast is smooth, sometimes small incisions could be observed. The single pyrenoid is medium large, spherical or broadly ellipsoidal, surrounded by several starch grains and placed in the thick part of chloroplast. Nucleus is placed in the anterior part of cell. Eyespot is small, dot-like, anterior to equator. Protoplast is often withdrawn from the cell wall. The vegetative cells (flagellated and immobile) are surrounded by layered mucilage and frequently immobile. Asexual reproduction by zoospores formation. Zoosporangia contain mostly 2–8, rarely 16 zoospores, and are 13.3–22.7 μm in diameter. The morphology of zoospores is similar to that of the vegetative cells. Sexual reproduction not observed.
<taxonomicNameid="4C415CBFFFC96638FEBBFBB95499B470"authority="Korshikov ex Pascher"authorityName="Pascher"box="[337,739,1032,1057]"class="Chlorophyceae"family="Chlamydomonadaceae"genus="Chlamydomonas"kingdom="Plantae"order="Chlamydomonadales"pageId="4"pageNumber="25"phylum="Chlorophyta"rank="species"species="gelatinosa">
<taxonomicNameid="4C415CBFFFC96638FD79F85055DFB7A7"authorityName="Pröschold & Darienko & Krienitz & Coleman"authorityYear="2018"box="[659,933,2017,2038]"class="Chlorophyceae"family="Chlamydomonadaceae"genus="Chlamydomonas"kingdom="Plantae"order="Chlamydomonadales"pageId="4"pageNumber="25"phylum="Chlorophyta"rank="species"species="schloesseri"status="sp. nov.">
<materialsCitationid="3B292D61FFC86639FF57FF2E5222B08D"collectionCode="SAG"country="Germany"location="University of Gottingen"pageId="5"pageNumber="26"specimenCode="SAG 2485"specimenCount="1"typeStatus="holotype">
<specimenCodeid="DBE78F47FFC86639FD77FF2E556CB0E9"box="[669,790,159,184]"collectionCode="SAG"country="Germany"httpUri="http://grbio.org/cool/2vvw-hn58"name="Sammlung von Algenkulturen at Universitat Gottingen"pageId="5"pageNumber="26">SAG 2485</specimenCode>
is permanently cryopreserved in a metabolically inactive state (cryopreserved in liquid nitrogen) in the
<collectionCodeid="ED50BFF9FFC86639FD60FF7254BFB08D"box="[650,709,195,220]"country="Germany"httpUri="http://grbio.org/cool/2vvw-hn58"name="Sammlung von Algenkulturen at Universitat Gottingen"pageId="5"pageNumber="26">SAG</collectionCode>
,
<locationid="8E9E71E7FFC86639FD3AFF7255A5B08D"LSID="urn:lsid:plazi:treatment:03E8962AFFC96639FF62FDCB542FB285:8E9E71E7FFC86639FD3AFF7255A5B08D"box="[720,991,195,220]"country="Germany"name="University of Gottingen"pageId="5"pageNumber="26">University of Göttingen</location>
<figureCitationid="137A3BB9FFC86639FCA3FF5655E7B151"box="[841,925,231,256]"captionStart="FIGURE 1"captionStartId="4.[136,229,2017,2038]"captionTargetBox="[155,1432,1150,1988]"captionTargetId="figure-357@4.[155,1433,1150,1988]"captionTargetPageId="4"captionText="FIGURE 1. Morphology and phenotypic plasticity of Chlamydomonas schloesseri sp. nov. A–J. vegetative cells, arrow in F. marked the eyespot; K–O. different sporangia; scale bar = 10 μm."figureDoi="http://doi.org/10.5281/zenodo.13702994"httpUri="https://zenodo.org/record/13702994/files/figure.png"pageId="5"pageNumber="26">Fig. 1A</figureCitation>
<emphasisid="B935FB2EFFC86639FF57FEBA57D4B175"bold="true"box="[189,430,267,292]"pageId="5"pageNumber="26">ITS-2 DNA Barcode</emphasis>
:
<collectionCodeid="ED50BFF9FFC86639FE57FEBA579DB175"box="[445,487,267,292]"country="Spain"lsid="urn:lsid:biocol.org:col:13287"name="Institut Botànic de Barcelona"pageId="5"pageNumber="26"type="Herbarium">BC</collectionCode>
<figureCitationid="137A3BB9FFC86639FDFEFEBA5422B175"box="[532,600,267,292]"captionStart="FIGURE 7"captionStartId="11.[136,229,1872,1893]"captionTargetBox="[170,1418,187,1843]"captionTargetId="figure-14@11.[170,1418,187,1844]"captionTargetPageId="11"captionText="FIGURE 7. Comparison of the conserved region of ITS-2 among the species of Chlamydomonas. Extraction of this region and translation into a number code for its usage as barcode (extracted bases highlighted with an asterisk). Number code for each base pair: 1 = A-U; 2 = U-A; 3 = G-C; 4 = C-G; 5 = G•U; 6 = U•G; 7 = mismatch."figureDoi="http://doi.org/10.5281/zenodo.13703008"httpUri="https://zenodo.org/record/13703008/files/figure.png"pageId="5"pageNumber="26">Fig. 7</figureCitation>
: The species was named in honor of Prof. Dr. Uwe Gert Schlösser for his contribution to phycology and his further development of the Culture Collection of Algae, University of Göttingen (SAG).
The three investigated strains are morphologically identical and differ mainly from other closely related species by the presence of mucilage surrounding the vegetative cells in
<taxonomicNameid="4C415CBFFFC86639FCBEFE2A558AB1E5"authorityName="Pröschold & Darienko & Krienitz & Coleman"authorityYear="2018"box="[852,1008,411,436]"class="Chlorophyceae"family="Chlamydomonadaceae"genus="Chlamydomonas"kingdom="Plantae"order="Chlamydomonadales"pageId="5"pageNumber="26"phylum="Chlorophyta"rank="species"species="schloesseri">
<tableCitationid="C6C31287FFC86639FCF6FE0E5509B189"box="[796,883,447,472]"captionStart="TABLE 1"captionStartId="2.[136,224,377,400]"captionText="TABLE 1. Comparison of diacritical morphological features among the species of Chlamydomonas."pageId="5"pageNumber="26">Table 1</tableCitation>
. As shown in
<tableCitationid="C6C31287FFC86639FBCAFE0E52FDB189"box="[1056,1159,447,472]"captionStart="TABLE 1"captionStartId="2.[136,224,377,400]"captionText="TABLE 1. Comparison of diacritical morphological features among the species of Chlamydomonas."pageId="5"pageNumber="26">Table S1</tableCitation>
using the identification keys of Ettl (1976, 1983). The three strains SAG 81.72, SAG 7.73 and NIES-2462 matched in morphology with original description of
<bibRefCitationid="EFD05ACDFFC86639FE6CFD9A5457B215"author="Pascher, A."box="[390,557,555,580]"pageId="5"pageNumber="26"pagination="1 - 506"refId="ref10302"refString="Pascher, A. (1927) Volvocales. In: Pascher, A. (Ed.) Susswasser-Flora Deutschlands, Osterreichs und der Schweiz. Heft 4. Gustav Fischer, Jena, pp. 1 - 506."type="book chapter"year="1927">Pascher (1927)</bibRefCitation>
<bibRefCitationid="EFD05ACDFFC86639FBC2FD9A52E0B215"author="Ettl"box="[1064,1178,555,580]"pageId="5"pageNumber="26"pagination="271 - 430"refId="ref8670"refString="Ettl, H. (1965) Beitrag zur Kenntnis der Morphologie der Gattung Chlamydomonas Ehrenberg. Archiv fur Protistenkunde 107: 271 - 430."type="journal article"year="1965">Ettl (1965</bibRefCitation>
, 1976). The strains of
<taxonomicNameid="4C415CBFFFC86639FA78FD9A577EB239"authorityName="Pröschold & Darienko & Krienitz & Coleman"authorityYear="2018"class="Chlorophyceae"family="Chlamydomonadaceae"genus="Chlamydomonas"kingdom="Plantae"order="Chlamydomonadales"pageId="5"pageNumber="26"phylum="Chlorophyta"rank="species"species="schloesseri">
using the keys of Ettl, but the comparison with original descriptions of this species by Korshikov in
<bibRefCitationid="EFD05ACDFFC86639FE72FDC25445B2DD"author="Pascher, A."box="[408,575,627,652]"pageId="5"pageNumber="26"pagination="1 - 506"refId="ref10302"refString="Pascher, A. (1927) Volvocales. In: Pascher, A. (Ed.) Susswasser-Flora Deutschlands, Osterreichs und der Schweiz. Heft 4. Gustav Fischer, Jena, pp. 1 - 506."type="book chapter"year="1927">Pascher (1927)</bibRefCitation>
and
<bibRefCitationid="EFD05ACDFFC86639FD99FDC25540B2DD"author="Korshikov, O. A."box="[627,826,627,652]"pageId="5"pageNumber="26"pagination="1 - 183"refId="ref9370"refString="Korshikov, O. A. (1938) Volvocineae. In: Roll, Y. V. (Ed.) Vyznacnyk prisnovodnych vodorostej Ukrainskoj RSR. Akad. Nauk USSR, Kyiv, pp. 1 - 183. [in Ukrainian]"type="book chapter"year="1938">Korshikov (1938)</bibRefCitation>
showed clear differences in morphology. The vegetative cells of
