<documentid="052AB78B3722B6CECBC446CBFFF34EF7"ID-DOI="10.11646/phytotaxa.365.1.1"ID-ISSN="1179-3163"ID-Zenodo-Dep="13704923"IM.bibliography_approvedBy="felipe"IM.illustrations_approvedBy="felipe"IM.materialsCitations_approvedBy="felipe"IM.metadata_approvedBy="felipe"IM.tables_requiresApprovalFor="GgImagineBatch"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="felipe"checkinTime="1725584015738"checkinUser="felipe"docAuthor="Mai, Truc, Johansen, Jeffrey R., Pietrasiak, Nicole, Bohunická, Markéta & Martin, Michael P."docDate="2018"docId="03BFDE646C4FFFA7009AF8BAFCEEFBF7"docLanguage="en"docName="phytotaxa.365.1.1.pdf"docOrigin="Phytotaxa 365 (1)"docSource="http://dx.doi.org/10.11646/phytotaxa.365.1.1"docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article"docStyleId="96748F8F1B6C902996E134952A3A36B9"docStyleName="Phytotaxa.2014-.journal_article"docStyleVersion="13"docTitle="Timaviella obliquedivisa Mai, Johansen et Bohunicka 2018, sp. nov."docType="treatment"docVersion="2"lastPageNumber="36"masterDocId="FF86A61C6C6EFF840012FFAAFF98FF96"masterDocTitle="Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species"masterLastPageNumber="59"masterPageNumber="1"pageNumber="34"updateTime="1725596029488"updateUser="ExternalLinkService"zenodo-license-figures="UNSPECIFIED"zenodo-license-treatments="UNSPECIFIED">
<mods:titleid="86671CEA40BBFE832E9C748F805FDB85">Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species</mods:title>
<mods:affiliationid="740B578B99FBBF9AB6296D1BDB79EBBC">Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA & Department of Plant and Environmental Sciences, New Mexico State University, Skeen Hall Room N 127, P. O Box 30003 MSC 3 Q, Las Cruces, New Mexico 88003, USA.</mods:affiliation>
<mods:affiliationid="512C9FD0AA792EF21A9D620CF95FE9CF">Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA & Department of Botany, Faculty of Science, University of South Bohemia, 31 Branišovská, 37005 České Budějovice, Czech Republic</mods:affiliation>
<mods:affiliationid="1190BD6EC824F05CB5BC3DB55B09B430">Department of Plant and Environmental Sciences, New Mexico State University, Skeen Hall Room N 127, P. O Box 30003 MSC 3 Q, Las Cruces, New Mexico 88003, USA.</mods:affiliation>
<mods:namePartid="D12FD65855A0B21E488324CC4D4F799B">Martin, Michael P.</mods:namePart>
<mods:affiliationid="9FFAE672CF282025A564B1A072AFA765">Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA</mods:affiliation>
<taxonomicNameid="4C1614F16C4FFFA5009AF8BAFD62F8BF"authority="Mai, Johansen et Bohunicka"authorityName="Mai, Johansen et Bohunicka"authorityYear="2018"box="[136,762,1808,1833]"class="Cyanobacteriia"family="Oculatellaceae"genus="Timaviella"higherTaxonomySource="GBIF"kingdom="Bacteria"order="Synechococcales"pageId="33"pageNumber="34"phylum="Cyanobacteria"rank="species"species="obliquedivisa"status="sp. nov.">
but differing in the profuse single and double false-branching and the coloration of trichomes, as well as the shorter D1-D1’ helix. V3 helix almost identical in shape to V3 helix of
<figureCitationid="132D73F76C4FFFA50274F8D6FD2EF803"box="[614,694,1916,1941]"captionStart="FIGURE 9"captionStartId="19.[136,229,1958,1979]"captionTargetBox="[229,912,769,1871]"captionTargetId="figure-135@19.[151,1436,768,1875]"captionTargetPageId="19"captionText="FIGURE 9. V3 helices of species described in Oculatellaceae. All species described have this structure. The V3 helix of Cartusia aeruginosa is missing because we do not have the full length of its ITS region."figureDoi="http://doi.org/10.5281/zenodo.13704943"httpUri="https://zenodo.org/record/13704943/files/figure.png"pageId="33"pageNumber="34">Fig. 9j</figureCitation>
<figureCitationid="132D73F76C4FFFA502EBF80AFCCEF82F"box="[761,854,1952,1977]"captionStart="FIGURE 6"captionStartId="15.[136,229,1976,1997]"captionTargetBox="[157,1425,273,1857]"captionTargetId="figure-14@15.[151,1436,267,1882]"captionTargetPageId="15"captionText="FIGURE 6. D1-D1’ stems of species described in Oculatellaceae.The stem structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here."figureDoi="http://doi.org/10.5281/zenodo.13704935"httpUri="https://zenodo.org/record/13704935/files/figure.png"pageId="33"pageNumber="34">Figs. 6k</figureCitation>
<figureCitationid="132D73F76C4FFFA50370F80AFCE2F82F"box="[866,890,1952,1977]"captionStart="FIGURE 7"captionStartId="17.[136,229,1928,1949]"captionTargetBox="[314,1274,759,1806]"captionTargetId="figure-238@17.[311,1276,751,1831]"captionTargetPageId="17"captionText="FIGURE 7. Box B helices of species described in Oculatellaceae. The structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here."figureDoi="http://doi.org/10.5281/zenodo.13704937"httpUri="https://zenodo.org/record/13704937/files/figure.png"pageId="33"pageNumber="34">7j</figureCitation>
<figureCitationid="132D73F76C4FFFA50394F80AFC3CF82F"box="[902,932,1952,1977]"captionStart="FIGURE 8"captionStartId="18.[136,229,1187,1208]"captionTargetBox="[152,1434,425,1107]"captionTargetId="figure-143@18.[151,1436,422,1162]"captionTargetPageId="18"captionText="FIGURE 8. V2 helices of species described in Oculatellaceae. Several species do not have this structure, including Pegethrix convoluta, P. indistincta, Antartic Pegethrix species, Cartusia fontana¸ Kaiparowitsia implicata."figureDoi="http://doi.org/10.5281/zenodo.13704941"httpUri="https://zenodo.org/record/13704941/files/figure.png"pageId="33"pageNumber="34">8h</figureCitation>
<figureCitationid="132D73F76C4FFFA503A2F80AFC51F82F"box="[944,969,1952,1977]"captionStart="FIGURE 9"captionStartId="19.[136,229,1958,1979]"captionTargetBox="[229,912,769,1871]"captionTargetId="figure-135@19.[151,1436,768,1875]"captionTargetPageId="19"captionText="FIGURE 9. V3 helices of species described in Oculatellaceae. All species described have this structure. The V3 helix of Cartusia aeruginosa is missing because we do not have the full length of its ITS region."figureDoi="http://doi.org/10.5281/zenodo.13704943"httpUri="https://zenodo.org/record/13704943/files/figure.png"pageId="33"pageNumber="34">9j</figureCitation>
Colony radially spreading, compact, firm, leathery, sometimes mounded, dark green, with yellowed margins near senescence. Filaments, untapered to slightly tapered (
<figureCitationid="132D73F76C4FFFA503E4F842FBE7F797"box="[1014,1151,2024,2049]"captionStart="FIGURE 17"captionStartId="34.[136,229,1993,2014]"captionTargetBox="[245,1342,350,1965]"captionTargetId="figure-93@34.[245,1342,350,1965]"captionTargetPageId="34"captionText="FIGURE 17. Timaviella obliquedivisa. A–B. Slightly tapering filaments. C–E. Consecutive double and single false-branches in filaments resulting in branch-like structures. F–G. Cell division in oblique angles, causing geminate, knot-like branching or resembling to truebranching. H. Compact coiling of trichomes within sheath. Scale bar 10μm in 1000X magnification."figureDoi="http://doi.org/10.5281/zenodo.13704963"httpUri="https://zenodo.org/record/13704963/files/figure.png"pageId="33"pageNumber="34">Figs. 17a–b</figureCitation>
<figureCitationid="132D73F76C4FFFA50185F7A6FD84F7B3"box="[407,540,2060,2085]"captionStart="FIGURE 17"captionStartId="34.[136,229,1993,2014]"captionTargetBox="[245,1342,350,1965]"captionTargetId="figure-93@34.[245,1342,350,1965]"captionTargetPageId="34"captionText="FIGURE 17. Timaviella obliquedivisa. A–B. Slightly tapering filaments. C–E. Consecutive double and single false-branches in filaments resulting in branch-like structures. F–G. Cell division in oblique angles, causing geminate, knot-like branching or resembling to truebranching. H. Compact coiling of trichomes within sheath. Scale bar 10μm in 1000X magnification."figureDoi="http://doi.org/10.5281/zenodo.13704963"httpUri="https://zenodo.org/record/13704963/files/figure.png"pageId="33"pageNumber="34">Figs. 17c–e</figureCitation>
), 2.0–3.2 (3.9) μm wide. Sheath usually thin, soft, colorless, rarely extended past trichome apex), up to 1.3 μm wide. Trichomes false branched, with some branches erect and almost perpendicular to the original axis of the trichome (
<figureCitationid="132D73F76C4CFFA60210FF6EFDE4FF4B"box="[514,636,196,221]"captionStart="FIGURE 17"captionStartId="34.[136,229,1993,2014]"captionTargetBox="[245,1342,350,1965]"captionTargetId="figure-93@34.[245,1342,350,1965]"captionTargetPageId="34"captionText="FIGURE 17. Timaviella obliquedivisa. A–B. Slightly tapering filaments. C–E. Consecutive double and single false-branches in filaments resulting in branch-like structures. F–G. Cell division in oblique angles, causing geminate, knot-like branching or resembling to truebranching. H. Compact coiling of trichomes within sheath. Scale bar 10μm in 1000X magnification."figureDoi="http://doi.org/10.5281/zenodo.13704963"httpUri="https://zenodo.org/record/13704963/files/figure.png"pageId="34"pageNumber="35">Fig. 17f–h</figureCitation>
<figureCitationid="132D73F76C4CFFA60384FF42FC6FFE97"box="[918,1015,232,257]"captionStart="FIGURE 17"captionStartId="34.[136,229,1993,2014]"captionTargetBox="[245,1342,350,1965]"captionTargetId="figure-93@34.[245,1342,350,1965]"captionTargetPageId="34"captionText="FIGURE 17. Timaviella obliquedivisa. A–B. Slightly tapering filaments. C–E. Consecutive double and single false-branches in filaments resulting in branch-like structures. F–G. Cell division in oblique angles, causing geminate, knot-like branching or resembling to truebranching. H. Compact coiling of trichomes within sheath. Scale bar 10μm in 1000X magnification."figureDoi="http://doi.org/10.5281/zenodo.13704963"httpUri="https://zenodo.org/record/13704963/files/figure.png"pageId="34"pageNumber="35">Fig. 17f</figureCitation>
), 2.0–2.9 μm wide. Necridia present, hormogonia rare. Cells cylindrical, shorter than wide to longer than wide, with parietal thylakoids, often with one large central granule, 1.8–2.7–(3.7) μm long.
<taxonomicNameid="4C1614F16C4CFFA6011DF863FE63F848"authorityName="Mai, Johansen et Bohunicka"authorityYear="2018"box="[271,507,1993,2014]"class="Cyanobacteriia"family="Oculatellaceae"genus="Timaviella"higherTaxonomySource="GBIF"kingdom="Bacteria"order="Synechococcales"pageId="34"pageNumber="35"phylum="Cyanobacteria"rank="species"species="obliquedivisa">
. A–B. Slightly tapering filaments. C–E. Consecutive double and single false-branches in filaments resulting in branch-like structures. F–G. Cell division in oblique angles, causing geminate, knot-like branching or resembling to truebranching. H. Compact coiling of trichomes within sheath. Scale bar 10μm in 1000X magnification.
D1-D1’ helix 63 nucleotides long, with a 3’ unilateral bulge of 8 nucleotides (5’-CAUCCCAA-3’). Mid-helix with a pair of nucleotide mismatch of U/U and two internal loops at position 14–15/41–42 and 20–23/32–36 which is separated from the terminal loop by a 5’-GC:GC-3’ clamp. Terminal loop having sequence 5’-GAAA-3’ (
<figureCitationid="132D73F76C4DFFA70558FF42FA07FE97"box="[1354,1439,232,257]"captionStart="FIGURE 6"captionStartId="15.[136,229,1976,1997]"captionTargetBox="[157,1425,273,1857]"captionTargetId="figure-14@15.[151,1436,267,1882]"captionTargetPageId="15"captionText="FIGURE 6. D1-D1’ stems of species described in Oculatellaceae.The stem structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here."figureDoi="http://doi.org/10.5281/zenodo.13704935"httpUri="https://zenodo.org/record/13704935/files/figure.png"pageId="35"pageNumber="36">Fig. 6k</figureCitation>
). Box B helix 50 nucleotides long, with several internal loops at position 5/44–46, 8–9/41 and 25–25/31–35, an internal mismatch 5/44–46 probably due to an insertion of an adenine residue. Terminal loop 5 nucleotides long, 5’-UUAAU-3’ (
<figureCitationid="132D73F76C4DFFA70082FEFEFF46FEFB"box="[144,222,340,365]"captionStart="FIGURE 7"captionStartId="17.[136,229,1928,1949]"captionTargetBox="[314,1274,759,1806]"captionTargetId="figure-238@17.[311,1276,751,1831]"captionTargetPageId="17"captionText="FIGURE 7. Box B helices of species described in Oculatellaceae. The structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here."figureDoi="http://doi.org/10.5281/zenodo.13704937"httpUri="https://zenodo.org/record/13704937/files/figure.png"pageId="35"pageNumber="36">Fig. 7j</figureCitation>
<figureCitationid="132D73F76C4DFFA7042EFEFEFB17FEFB"box="[1084,1167,340,365]"captionStart="FIGURE 8"captionStartId="18.[136,229,1187,1208]"captionTargetBox="[152,1434,425,1107]"captionTargetId="figure-143@18.[151,1436,422,1162]"captionTargetPageId="18"captionText="FIGURE 8. V2 helices of species described in Oculatellaceae. Several species do not have this structure, including Pegethrix convoluta, P. indistincta, Antartic Pegethrix species, Cartusia fontana¸ Kaiparowitsia implicata."figureDoi="http://doi.org/10.5281/zenodo.13704941"httpUri="https://zenodo.org/record/13704941/files/figure.png"pageId="35"pageNumber="36">Fig. 8h</figureCitation>
). V3 helix 62 nucleotides long, with several internal loops at positions 4/55–57, 11–12/47–50 and 23/34–35, separated from the terminal loop by a 5’-GU:AG-3’ clamp (
<figureCitationid="132D73F76C4DFFA7019CFE36FE44FE23"box="[398,476,412,437]"captionStart="FIGURE 9"captionStartId="19.[136,229,1958,1979]"captionTargetBox="[229,912,769,1871]"captionTargetId="figure-135@19.[151,1436,768,1875]"captionTargetPageId="19"captionText="FIGURE 9. V3 helices of species described in Oculatellaceae. All species described have this structure. The V3 helix of Cartusia aeruginosa is missing because we do not have the full length of its ITS region."figureDoi="http://doi.org/10.5281/zenodo.13704943"httpUri="https://zenodo.org/record/13704943/files/figure.png"pageId="35"pageNumber="36">Fig. 9j</figureCitation>
<dateid="FFA849B26C4DFFA70154FD86FE66FDD3"box="[326,510,556,581]"pageId="35"pageNumber="36"value="2006-08-15">15 August 2006</date>
by Markéta Bohunická. Hanging garden (concave wet rock face in a grotto with vascular plants hanging from the rock) on a sandstone rock wall in the Carmel-Page formation, partly covered with organic debris, mosses and vascular plants, in the
<collectingRegionid="49D2A1906C4DFFA703DAFDDEFB99FD1B"box="[968,1025,628,653]"country="United States of America"name="Utah"pageId="35"pageNumber="36">Utah</collectingRegion>
,
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<materialsCitationid="3B7E652F6C4DFFA700AFFD32FE8FFD43"collectionCode="BRY"collectorName="Monte L. Bean Museum"location="Utah"pageId="35"pageNumber="36"specimenCode="BRY37787"specimenCount="1"stateProvince="Utah"typeStatus="holotype">
<specimenCodeid="DBB0C7096C4DFFA70204FD32FD05FD27"box="[534,669,664,689]"collectionCode="BRY"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:15673"lsid="urn:lsid:biocol.org:col:15673"name="Brigham Young University - S.L. Welsh Herbarium"pageId="35"pageNumber="36"type="Herbarium">BRY37787</specimenCode>
!, Herbarium for Nonvascular Cryptogams,
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, Provo,
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<collectionCodeid="ED07F7B76C4DFFA70217FD4AFDDAFD6F"box="[517,578,736,761]"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:15673"lsid="urn:lsid:biocol.org:col:15673"name="Brigham Young University - S.L. Welsh Herbarium"pageId="35"pageNumber="36"type="Herbarium">BRY</collectionCode>
37788!, Herbarium for Nonvascular Cryptogams, Monte L. Bean Museum, Provo,
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GSE-PSE-MK28-08A, Algal Culture Collection at John Carroll University, Cleveland,
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. Additional reference strain: GSE-PSE-MK23-08B, Algal Culture Collection at John Carroll University, Cleveland,
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Attenuated trichomes, erect false branches, and oblique division leading to biseriate trichomes are the defining morphological characteristics of
<taxonomicNameid="4C1614F16C4DFFA7032EFC12FC6AFC47"authorityName="Mai, Johansen et Bohunicka"authorityYear="2018"box="[828,1010,952,977]"class="Cyanobacteriia"family="Oculatellaceae"genus="Timaviella"higherTaxonomySource="GBIF"kingdom="Bacteria"order="Synechococcales"pageId="35"pageNumber="36"phylum="Cyanobacteria"rank="species"species="obliquedivisa">
. No previously described species is a match for these characteristics (
<bibRefCitationid="EF8712836C4DFFA701FAFC76FD2BFC63"author="Sciuto, K. & Moschin, E. & Moro, I."box="[488,691,988,1013]"pageId="35"pageNumber="36"pagination="285 - 323"refId="ref37019"refString="Sciuto, K., Moschin, E. & Moro, I. (2017) Cryptic cyanobacterial diversity in the Giant Cave (Trieste, Italy): the new genus Timaviella (Leptolyngbyaceae). Cryptogamie, Algologie 38: 285 - 323. https: // doi. org / 10.7872 / crya / v 38. iss 4.2017.285"type="journal article"year="2017">
). The secondary structures of the conserved ITS domains are unique in comparison with the same structures from other
<taxonomicNameid="4C1614F16C4DFFA702D6FBAAFCF4FB8F"authorityName="Mai et Johansen"authorityYear="2018"box="[708,876,1024,1049]"class="Cyanobacteriia"family="Oculatellaceae"higherTaxonomySource="GBIF"kingdom="Bacteria"order="Synechococcales"pageId="35"pageNumber="36"phylum="Cyanobacteria"rank="family">Oculatellaceae</taxonomicName>
, but without exceptional features; they look similar to other
<taxonomicNameid="4C1614F16C4DFFA700F7FB8EFE12FBAB"authorityName="Mai et Johansen"authorityYear="2018"box="[229,394,1060,1085]"class="Cyanobacteriia"family="Oculatellaceae"higherTaxonomySource="GBIF"kingdom="Bacteria"order="Synechococcales"pageId="35"pageNumber="36"phylum="Cyanobacteria"rank="family">Oculatellaceae</taxonomicName>
in the basal clamps and absence of side branches. The conserved domains of the 16S–23S had secondary structures unique to this species (see
<bibRefCitationid="EF8712836C4DFFA702B2FBE2FCF2FBF7"author="Sciuto, K. & Moschin, E. & Moro, I."box="[672,874,1096,1121]"pageId="35"pageNumber="36"pagination="285 - 323"refId="ref37019"refString="Sciuto, K., Moschin, E. & Moro, I. (2017) Cryptic cyanobacterial diversity in the Giant Cave (Trieste, Italy): the new genus Timaviella (Leptolyngbyaceae). Cryptogamie, Algologie 38: 285 - 323. https: // doi. org / 10.7872 / crya / v 38. iss 4.2017.285"type="journal article"year="2017">
