<documentid="A7782F2338FE55E769935C5190F7AEF8"ID-CLB-Dataset="306268"ID-DOI="10.11646/zootaxa.5541.4.7"ID-GBIF-Dataset="9f6fdc28-1801-41f4-9f73-dd0c9bb1f42a"ID-ISSN="1175-5326"ID-Zenodo-Dep="14241302"ID-ZooBank="8436A6AB-981D-4C2C-97E8-5BDFC7657A36"IM.bibliography_approvedBy="karina"IM.illustrations_approvedBy="karina"IM.materialsCitations_approvedBy="karina"IM.metadata_approvedBy="felipe"IM.tables_approvedBy="karina"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="karina"checkinTime="1732836557263"checkinUser="plazi"docAuthor="Justo, Marcia Cristina Nascimento, Martins, Williane Maria De Oliveira & Cohen, Simone Chinicz"docDate="2024"docId="8C6987B2FFAE2D2BFF26BEC6FD2BB849"docLanguage="en"docName="zootaxa.5541.4.7.pdf"docOrigin="Zootaxa 5541 (4)"docSource="http://dx.doi.org/10.11646/zootaxa.5360.3.8"docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article"docStyleId="647186512141C8FC8976D5BCC54AEB7D"docStyleName="Zootaxa.2013-.journal_article"docStyleVersion="9"docTitle="Demidospermus bifurcatus Justo, Martins & Cohen, 2024, sp. nov."docType="treatment"docVersion="6"lastPageNumber="552"masterDocId="7050FFCAFFAD2D28FFB1BC61FFEABF0A"masterDocTitle="Three new species of Demidospermus (Monopisthocotyla: Dactylogyridae) parasitizing Ageneiosus inermis (Siluriformes: Auchenipteridae) from western Amazon, Brazil"masterLastPageNumber="562"masterPageNumber="549"pageNumber="552"updateTime="1733758152690"updateUser="ExternalLinkService"zenodo-license-document="CLOSED">
<mods:titleid="592FCB8B1552DE88B3A41719BED91D33">Three new species of Demidospermus (Monopisthocotyla: Dactylogyridae) parasitizing Ageneiosus inermis (Siluriformes: Auchenipteridae) from western Amazon, Brazil</mods:title>
<mods:affiliationid="96FBEA00BA45A3D55DA1BFE91DB73281">Laboratório de Helmintos Parasitos de Peixes, Instituto Oswaldo Cruz, FIOCRUZ. Avenida Brasil 4365, Rio de Janeiro, RJ, 21040 - 900, Brazil</mods:affiliation>
<mods:namePartid="DE9995E8CE9C8D913E08B90171F77F21">Martins, Williane Maria De Oliveira</mods:namePart>
<mods:affiliationid="D8466DE9158722724FD1AC2CF9445600">Instituto Federal do Acre, IFAC. Rodovia AC- 90, Transacreana, KM 20, Rio Branco-Acre, 69912 - 290, Brazil.</mods:affiliation>
<mods:affiliationid="D968AE25385B044EF332CE8BAA0F3ED2">Laboratório de Helmintos Parasitos de Peixes, Instituto Oswaldo Cruz, FIOCRUZ. Avenida Brasil 4365, Rio de Janeiro, RJ, 21040 - 900, Brazil</mods:affiliation>
<taxonomicNameid="C3C04D27FFAE2D2BFF26BEC6FE34BDC8"authorityName="Justo & Martins & Cohen"authorityYear="2024"box="[151,478,679,706]"class="Monogenea"family="Ancylodiscoididae"genus="Demidospermus"kingdom="Animalia"order="Dactylogyridea"pageId="3"pageNumber="552"phylum="Platyhelminthes"rank="species"species="bifurcatus"status="sp. nov.">
<figureCitationid="9CFB2A21FFAE2D2BFF2EBEADFEC0BDEC"box="[159,298,716,742]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Figs. 2 A–G</figureCitation>
<paragraphid="047F36A4FFAE2D2BFF76BF59FE39BC58"blockId="3.[151,1437,788,1859]"box="[199,467,824,850]"pageId="3"pageNumber="552">Site of infestation: Gills</paragraph>
<paragraphid="047F36A4FFAE2D2BFF76BFE1FA6ABC90"blockId="3.[151,1437,788,1859]"box="[199,1408,896,922]"pageId="3"pageNumber="552">Parasitological indexes: Total number of hosts: 1; number of infested hosts: 1; total number of parasites: 10.</paragraph>
<collectionCodeid="62D1AE61FFAE2D2BFC34BFC5FC08BCB4"box="[901,994,932,958]"country="Brazil"httpUri="http://grbio.org/cool/sv7j-t59d"name="Helminthological Collection of Oswaldo Cruz Institute (Coleccion. Helmintologica del Instituto Oswaldo Cruz)"pageId="3"pageNumber="552">CHIOC</collectionCode>
<paragraphid="047F36A4FFAE2D2BFF76BFA9FE94BB0C"blockId="3.[151,1437,788,1859]"pageId="3"pageNumber="552">Etymology: The specific name is a Latin adjective (bifurcatus = forked), due to the bifurcation in the distal portion of the MCO.</paragraph>
mounted in Hoyer’s medium). Body elongated, fusiform, 355–650 (537; n=8) long by 78–175 (126; n=8) wide at the level of the ovary. Cephalic region broad; cephalic lobes weakly developed, 3 bilateral pairs of head organs; cephalic glands indistinct. Subspherical eyespot granules scattered throughout cephalic region. Pharynx subspherical to spherical 40–62 (54; n=8) long by 28–57 (49; n=8); esophagus short, intestinal ceca confluent posterior to gonads. Male copulatory organ robust tube, inverted C-shaped, with a bisected tip at its distal end, irregular base, 69–111 (88; n=8); accessory piece robust, embracing the MCO in its full extension, 63–105 (79; n=8), proximal portion expanded forming two arms, one wider than the other, with a tendency to wrap around the base of the MCO (
<figureCitationid="9CFB2A21FFAE2D2BFDF2B96DFD74BA2C"box="[579,670,1292,1318]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2A</figureCitation>
). Gonads in tandem; testis 187 (n=1) long; vas deferens looping left intestinal cecum. Seminal vesicle long, fusiform. Germarium pretesticular 75 (n=1) long. Slightly sclerotized vagina with bifurcated canal and ornate opening (
<figureCitationid="9CFB2A21FFAE2D2BFDCEB935FD33BA64"box="[639,729,1364,1390]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2B</figureCitation>
). Oviduct, ootype, and uterus not observed. Vitelline follicles densely distributed in trunk, not overlapping the reproductive organs. Peduncle inconspicuous. Haptor width similar to the body. Haptor sub-hexagonal, 71–215 (116; n= 8) wide. Ventral bar slightly curved 42–59 (50; n=8) long (
<figureCitationid="9CFB2A21FFAE2D2BFADEB9FDFF56BAD0"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2C</figureCitation>
); dorsal bar open V-shaped 44–59 (52; n=8) long (
<figureCitationid="9CFB2A21FFAE2D2BFD56B9A1FCA1BAD0"box="[743,843,1472,1498]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Figs. 2D</figureCitation>
). Ventral anchor with elongated superficial root; deep root rounded; straight shank, straight and long point, extending slightly beyond superficial root 28–35 (32; n=16), base 17–24 (19; n=16) (
<figureCitationid="9CFB2A21FFAE2D2BFE1EBA69FDE0B928"box="[431,522,1544,1570]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2E</figureCitation>
). Dorsal anchor with well-developed superficial root, deep root rounded, short, straight point extending slightly beyond superficial root, 22–35 (31; n=16), base 16–22 (19; n=16) (
<figureCitationid="9CFB2A21FFAE2D2BFB5ABA4DFAAAB94C"box="[1259,1344,1580,1606]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2F</figureCitation>
). Hooks similar in shape and size, pair 1, 15–21 (18; n=8), pair 2, 15–21 (19; n=8), pair 3, 15–22 (19; n=7), pair 4, 16–19 (18; n=5), pair 5, 17–22 (20; n=5), pair 6, 17–20 (19; n=4), pair 7, 17–19 (19; n=4), with curved point and delicate shaft, protruding thumb, expanded shank proximally; FH loop approximately ⅔ shank length (
<figureCitationid="9CFB2A21FFAE2D2BFB08BAF9FAF9B9B8"box="[1209,1299,1688,1714]"captionStart="FIGURE 2"captionStartId="4.[151,250,1286,1310]"captionTargetBox="[184,1404,194,1249]"captionTargetId="figure-28@4.[171,1417,181,1261]"captionTargetPageId="4"captionText="FIGURE 2. Demidospermus bifurcatus sp. nov. from Ageneiosus inermis. A. Copulatory complex, ventral view; B. Vagina; C. Ventral bar; D. Dorsal bar; E. Ventral anchor; F. Dorsal anchor; G. Hook. Scale bars: A, C, D = 30 µm; B, 10 µm; E, F = 20 µm; B, G =10 µm."figureDoi="http://doi.org/10.5281/zenodo.14241306"httpUri="https://zenodo.org/record/14241306/files/figure.png"pageId="3"pageNumber="552">Fig. 2G</figureCitation>
<taxonomicNameid="C3C04D27FFAE2D2BFEF4BADDFD99B9DC"authorityName="Justo & Martins & Cohen"authorityYear="2024"box="[325,627,1724,1750]"class="Monogenea"family="Ancylodiscoididae"genus="Demidospermus"kingdom="Animalia"order="Dactylogyridea"pageId="3"pageNumber="552"phylum="Platyhelminthes"rank="species"species="bifurcatus">
is characterized by a MCO formed by a robust tube, inverted C-shaped, with a distal end presenting a bisected tip and a robust accessory piece, embracing the MCO in its full extension, proximal portion to the base of the MCO expanded forming two arms; this morphology of the copulatory complex distinguishes the new species from all congeners.
