<documentid="702C4B421DB2B431EAE7D1CA291FB9BA"ID-DOI="10.1080/00222933.2024.2345927"ID-ISSN="1464-5262"ID-Zenodo-Dep="13219746"IM.bibliography_approvedBy="carolina"IM.illustrations_approvedBy="felipe"IM.materialsCitations_approvedBy="carolina"IM.metadata_approvedBy="felipe"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="carolina"checkinTime="1722843405104"checkinUser="plazi"docAuthor="Araujo, Thiago Q., Walsh, Elizabeth J., Wallace, Robert L. & Hochberg, Rick"docDate="2024"docId="03D3D10EFFA0FFE3FF4DF997D756FDE1"docLanguage="en"docName="JNATHIST.58.25-28.784-795.pdf"docOrigin="Journal of Natural History (J. Nat. Hist.) 58 (25 - 28)"docSource="http://dx.doi.org/10.1080/00222933.2024.2345927"docStyle="DocumentStyle:3C762F9783B768A90048B82ACD135A62.3:JNatHist.2017-.journal_article.0cover"docStyleId="3C762F9783B768A90048B82ACD135A62"docStyleName="JNatHist.2017-.journal_article.0cover"docStyleVersion="3"docTitle="Pompholyx faciemlarva Araujo, Walsh, Wallace and Hochberg 2024, sp. n."docType="treatment"docVersion="3"lastPageNumber="793"masterDocId="FFEAA976FFA2FFEAFFBFFFA2D61EFFA9"masterDocTitle="A new species of Pompholyx (Rotifera: Monogononta: Testudinellidae) from the United States"masterLastPageNumber="795"masterPageNumber="784"pageNumber="786"updateTime="1723486086844"updateUser="carolina"zenodo-license-document="CLOSED">
<mods:titleid="4F7876A2744FE1D2C5D9BD6EFA81A3E3">A new species of Pompholyx (Rotifera: Monogononta: Testudinellidae) from the United States</mods:title>
<mods:namePartid="4633D0B15A5FA00E63548E0D42153265">Walsh, Elizabeth J.</mods:namePart>
<mods:affiliationid="27DB03E5A57840D7D013ECFC9355B8B6">Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA;</mods:affiliation>
<taxonomicNameid="4C7A1B9BFFA0FFE8FF4DF997D5FCF9F9"authority="Araujo, Walsh, Wallace and Hochberg"authorityName="Araujo, Walsh, Wallace and Hochberg"authorityYear="2024"box="[242,994,1589,1616]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="2"pageNumber="786"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<locationid="8EA536C3FFA1FFE9FEAEFF55D724FEB9"LSID="urn:lsid:plazi:treatment:03D3D10EFFA0FFE3FF4DF997D756FDE1:8EA536C3FFA1FFE9FEAEFF55D724FEB9"box="[273,314,247,272]"municipality="The International Code of Zoological Nomenclature"name="The"pageId="3"pageNumber="787">The</location>
type specimen was documented, as is permitted digitally recorded: digital type documentation is allowed by
<collectingMunicipalityid="6BA1FA62FFA1FFE9FD8EFEB8D29EFE9A"box="[561,1152,282,307]"pageId="3"pageNumber="787">The International Code of Zoological Nomenclature</collectingMunicipality>
<locationid="8EA536C3FFA1FFE9FE16FE9ED224FEFC"LSID="urn:lsid:plazi:treatment:03D3D10EFFA0FFE3FF4DF997D756FDE1:8EA536C3FFA1FFE9FE16FE9ED224FEFC"box="[425,1082,316,341]"name="The International Commission on Zoological Nomenclature"pageId="3"pageNumber="787">The International Commission on Zoological Nomenclature</location>
<locationid="8EA536C3FFA1FFE9FCAAFEFDD520FED1"LSID="urn:lsid:plazi:treatment:03D3D10EFFA0FFE3FF4DF997D756FDE1:8EA536C3FFA1FFE9FCAAFEFDD520FED1"box="[789,830,351,376]"municipality="Smithsonian National Museum of Natural History"name="The"pageId="3"pageNumber="787">The</location>
type photomicrographs are available at the
<collectingMunicipalityid="6BA1FA62FFA1FFE9FEE9FE20D59FFE32"box="[342,897,386,411]"pageId="3"pageNumber="787">Smithsonian National Museum of Natural History</collectingMunicipality>
under accession numbers
<specimenCodeid="DBDCC863FFA1FFE9FF64FE06D792FE14"box="[219,396,420,445]"collectionCode="USNM"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871"lsid="urn:lsid:biocol.org:col:34871"name="Smithsonian Institution, National Museum of Natural History"pageId="3"pageNumber="787"type="Museum">USNM 1606876</specimenCode>
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,
<specimenCodeid="DBDCC863FFA1FFE9FCB3FE06D5DEFE14"box="[780,960,420,445]"collectionCode="USNM"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871"lsid="urn:lsid:biocol.org:col:34871"name="Smithsonian Institution, National Museum of Natural History"pageId="3"pageNumber="787"type="Museum">USNM 1606878</specimenCode>
,
<specimenCodeid="DBDCC863FFA1FFE9FC72FE06D29FFE14"box="[973,1153,420,445]"collectionCode="USNM"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871"lsid="urn:lsid:biocol.org:col:34871"name="Smithsonian Institution, National Museum of Natural History"pageId="3"pageNumber="787"type="Museum">USNM 1606879</specimenCode>
,
<specimenCodeid="DBDCC863FFA1FFE9FF1FFE65D744FE49"box="[160,346,455,480]"collectionCode="USNM"country="USA"httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871"lsid="urn:lsid:biocol.org:col:34871"name="Smithsonian Institution, National Museum of Natural History"pageId="3"pageNumber="787"type="Museum">USNM 1606880</specimenCode>
,
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<taxonomicNameid="4C7A1B9BFFA1FFE9FF1FFDC0D7BCFDD2"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[160,418,610,635]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="3"pageNumber="787"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
by the presence of transverse furrows on its lorica, the structure of the stalk gland (3-lobed), and the number and symmetrical arrangement of unci teeth in the trophi. There are 17 teeth on each side in the new species, compared to 17–20/18–21 (right/left) in
The description is based on an adult specimen of 110 µm (95–125 µm) body length. The body shape is ellipsoid, but with a flat anterior margin and rounded triangular posterior end. The corona is complete, ∞-shaped, and bears two dorsolateral red eyes (
<figureCitationid="13417C9DFFA1FFE9FF75FB28D6F2FB0A"box="[202,236,1162,1187]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="3"pageNumber="787">4D</figureCitation>
). Coronal cilia are approximately 8–20 µm long. In cross section, the smooth and mostly featureless lorica is arched dorsally and with flared lateral edges (
). A large transverse furrow extends laterally across the body on both dorsal and ventral sides around mid-body length. The furrow is present in all living specimens when swimming and does not change when animals are anesthetized; the furrow remains in preserved animals. An occipital convexity (edge) is present just posterior of the corona on the dorsal side. The ventral lorica surface is smooth, but narrower in width than the dorsal surface: it is 80 µm long and has an inverted triangle shape with a 53 µm wide anterior border and a 19 µm wide posterior tip. A pectoral concavity (notch) just posterior to the corona is present on the ventral surface (
). A ciliated sub-labium extends from the pectoral concavity during locomotion. The posterior end has a terminal 6 µm diameter cavity that leads to the cloaca (
<figureCitationid="13417C9DFFA1FFE9FCDEF9EED560F9CC"box="[865,894,1612,1637]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="3"pageNumber="787">4B</figureCitation>
). The cloaca has three openings to the blastocoel: one for the stalk gland, one to the anus, and one for oviposition (
<taxonomicNameid="4C7A1B9BFFA6FFEEFE41F9CDD4C3F92F"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[510,733,1647,1670]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="4"pageNumber="788"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<taxonomicNameid="4C7A1B9BFFA7FFEFFE4EF98BD4D1F9E9"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[497,719,1577,1600]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="5"pageNumber="789"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<taxonomicNameid="4C7A1B9BFFA4FFECFD7AFCC5D5BBFCD7"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[709,933,871,894]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="6"pageNumber="790"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
The corona is ∞-shaped (i.e., it resembles a number 8 lying on its side or a scuba diving mask) and ciliation is complete. Cilia appear to be of a mostly uniform length of 6– 18 μm. Small patches of presumable sensory cilia are present in the apical field. A ciliated sub-labium is present on the ventral surface just posterior of the mouth within the pectoral notch.
Terminal mouth is somewhat ventral in position, but still within the coronal field. The mouth leads to a 30 µm long ciliated buccal cavity that has an inverted pear-shape. The ciliated stomach has two salivary/gastric glands connected to the anterior part of the stomach. The intestine is 20 µm in total length and has two sphincters: one sphincter demarcates the transition from the stomach to the intestine; the second sphincter demarcates the transition from the intestine to the cloaca (
<figureCitationid="13417C9DFFA4FFECFF1FFA06D6CFFA14"box="[160,209,1444,1469]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="6"pageNumber="790">4B,E</figureCitation>
Trophi malleoramate. The rami are 13 µm long and have an elongatetriangular shape with rounded latero-ventral margins, delimiting large latero-ventral fenestrae (
<figureCitationid="13417C9DFFA4FFECFEA3F99CD791F9FE"box="[284,399,1598,1623]"captionStart="Figure 5"captionStartId="7.[160,225,874,897]"captionTargetBox="[165,1152,144,841]"captionTargetId="figure-219@7.[163,1153,142,843]"captionTargetPageId="7"captionText="Figure 5. Trophi of Pompholyx faciemlarva sp. n. (A–C) DIC microphotographs. (A) Frontal view; (B) focal plane between frontal and caudal view; (C) caudal view. (D,E) SEM false colour-coded microphotographs. (D) Caudal view; (E) slightly internal view of the ramus. Abbreviations: as, arched rami scleropili; dc, dorsal chamber; fu, fulcrum; mc, median chamber; r, ramus; rf, ramus fenestra; svc, subventral chambers; u, uncus; vc, ventral chamber."figureDoi="http://doi.org/10.5281/zenodo.13219756"httpUri="https://zenodo.org/record/13219756/files/figure.png"pageId="6"pageNumber="790">Figure 5D</figureCitation>
). The fulcrum is elongate and straight (11 µm). The unci plates consist of 16 right and 17 left (frontal view) weakly curved and strongly webbed asymmetrical teeth (lengths: 5–7 µm). The crescent-shaped manubria are 15 µm long and composed of superimposed dorsal, median, ventral, and small sub-ventral chambers (
<taxonomicNameid="4C7A1B9BFFA5FFEDFED9FCC8D459FC28"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[358,583,874,897]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="7"pageNumber="791"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<figureCitationid="13417C9DFFA5FFEDFF1FFB1CD6A1FB7E"box="[160,191,1214,1239]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="7"pageNumber="791">4D</figureCitation>
). Germarium not observed. Amictic eggs are oviposited one at a time and retained by the female at her posterior end. Each egg is approximately 50 µm in diameter and connected to an adhesive string that is secreted internally by a stalk gland and protrudes out the cloaca (
<figureCitationid="13417C9DFFA5FFEDFE61FA84D40DFA96"box="[478,531,1318,1343]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="7"pageNumber="791">4A,D</figureCitation>
<figureCitationid="13417C9DFFA5FFEDFC55FA32D219FA00"box="[1002,1031,1424,1449]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="7"pageNumber="791">4C</figureCitation>
<figureCitationid="13417C9DFFA5FFEDFC23FA58D5A6F9BA"box="[924,952,1530,1555]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="7"pageNumber="791">4B</figureCitation>
). The cells are not ova; their function is undetermined.
A stalk gland is present on the ventral side. The gland has at least three apical lobes (total length: 8 µm) and secretes a sticky thread (‘Gossesche thread’) approximately 80 µm long that passes through the cloaca to attach to a single amictic egg. At least two muscles insert on or close to the stalk gland and function to release or retract the adhesive thread, thereby moving the egg farther from or closer to the cloaca (
<figureCitationid="13417C9DFFABFFE3FBA8FF2CD253FF0E"box="[1047,1101,142,167]"captionStart="Figure 4"captionStartId="6.[160,225,871,894]"captionTargetBox="[164,1150,143,837]"captionTargetId="figure-228@6.[163,1153,142,839]"captionTargetPageId="6"captionText="Figure 4. DIC microphotograph of internal anatomy of Pompholyx faciemlarva sp. n. (A–D) Different focal planes of ventrolateral body view. Abbreviations: co, corona; e, eye; i, intestine; n, nucleus; pn, protonephridia; rc, round (unidentified) cells; sg, stalk gland; st, stomach; t, trophi; vit, vitellarium."figureDoi="http://doi.org/10.5281/zenodo.13219754"httpUri="https://zenodo.org/record/13219754/files/figure.png"pageId="9"pageNumber="793">4A,D</figureCitation>
). No more than one amictic egg was ever observed being carried by an adult female.
<taxonomicNameid="4C7A1B9BFFAAFFE2FD97F987D50CF995"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[552,786,1573,1596]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="8"pageNumber="792"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<emphasisid="B90EBC0AFFABFFE3FF1FFF54D7DCFEB9"bold="true"box="[160,450,246,272]"italics="true"pageId="9"pageNumber="793">Ecology and distribution</emphasis>
<taxonomicNameid="4C7A1B9BFFABFFE3FF1FFEBBD783FE9B"authorityName="Araujo & Walsh & Wallace & Hochberg"authorityYear="2024"box="[160,413,281,306]"class="Eurotatoria"family="Testudinellidae"genus="Pompholyx"kingdom="Animalia"order="Flosculariaceae"pageId="9"pageNumber="793"phylum="Rotifera"rank="species"species="faciemlarva"status="sp. nov.">
<collectingCountryid="F36D2088FFABFFE3FC6AFEBBD219FE9B"box="[981,1031,281,306]"name="United States of America"pageId="9"pageNumber="793">USA</collectingCountry>
). This pond has a rich submerged macrophyte community and the plankton was abundant, comprising at least 11 genera of rotifers and numerous other zooplanktonic taxa. The new species is common, but not abundant. Its collection from the surface waters (<
<bibRefCitationid="EFEB1DE9FFABFFE3FEA8FE4BD7BCFDAB"author="Gosse PH"box="[279,418,489,514]"pageId="9"pageNumber="793"pagination="18 - 24"refId="ref4374"refString="Gosse PH. 1850. Description of Asplanchna priodonta, an animal of the class rotifera. Ann Mag Nat Hist. 2: 18 - 24."type="journal article"year="1850">Gosse, 1850</bibRefCitation>
<bibRefCitationid="EFEB1DE9FFABFFE3FF1FFDAED72CFD8C"author="Gosse PH"box="[160,306,524,549]"pageId="9"pageNumber="793"pagination="197 - 203"refId="ref4402"refString="Gosse PH. 1851. XVIII. - A catalogue of Rotifera found in Britain; with descriptions of five new genera and thirty-two new species. Ann Mag Nat Hist. 8: 197 - 203. doi: 10.1080 / 03745486109496205."type="journal article"year="1851">Gosse, 1851</bibRefCitation>
<bibRefCitationid="EFEB1DE9FFABFFE3FD4CFDAED599FD8C"author="Gosse PH"box="[755,903,524,549]"pageId="9"pageNumber="793"pagination="197 - 203"refId="ref4402"refString="Gosse PH. 1851. XVIII. - A catalogue of Rotifera found in Britain; with descriptions of five new genera and thirty-two new species. Ann Mag Nat Hist. 8: 197 - 203. doi: 10.1080 / 03745486109496205."type="journal article"year="1851">Gosse, 1851</bibRefCitation>