<documentID-CLB-Dataset="96204"ID-DOI="http://dx.doi.org/10.3897/evolsyst.6.86308"ID-GBIF-Dataset="e0ec3d2c-462c-4e11-ac6e-ba5af9b94062"ID-Pensoft-Pub="2535-0730-2-135"ID-Pensoft-UUID="B3A8C24BC2FA5E7FAB4690A971314A03"ID-ZooBank="4DE05AA877614DAFB32A739315B60D95"ModsDocID="2535-0730-6-2-135"checkinTime="1657813446605"checkinUser="pensoft"docAuthor="Nakano, Takafumi"docDate="2022"docId="EAF586B05E8A507D9B36AE9243F04530"docLanguage="en"docName="EvolutSyst 6(2): 135-142"docOrigin="Evolutionary Systematics 6 (2)"docPubDate="2022-07-13"docSource="http://dx.doi.org/10.3897/evolsyst.6.86308"docTitle="Orobdella ibukifukuyamai Nakano 2022, sp. nov."docType="treatment"docUuid="824E7A4A-7FBC-49D7-B457-3EE3F40BBABA"docUuidSource="ZooBank"docVersion="5"id="B3A8C24BC2FA5E7FAB4690A971314A03"lastPageNumber="135"masterDocId="B3A8C24BC2FA5E7FAB4690A971314A03"masterDocTitle="A new sexannulate species of Orobdella (Hirudinea, Arhynchobdellida, Orobdellidae) from Kii-Oshima Island, Japan"masterLastPageNumber="142"masterPageNumber="135"pageNumber="135"updateTime="1732846927745"updateUser="ExternalLinkService">
<mods:titleid="DC6990D639A892BC3F0819EED1CAE030">A new sexannulate species of Orobdella (Hirudinea, Arhynchobdellida, Orobdellidae) from Kii-Oshima Island, Japan</mods:title>
<mods:affiliationid="D32DEE12ACA47AE87FFA7ACBF010F1DA">Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606 - 8502, Japan</mods:affiliation>
<figureCitationid="DD6B1BACD63883CCC597031E4492D656"captionStart="Figure 1"captionStartId="F1"captionText="Figure 1. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view; B. Ventral view; C. Dorsal view of live animal. Scale bars: 20 mm (same bar for A, B)."httpUri="https://binary.pensoft.net/fig/714572"pageId="0"pageNumber="135">Figs 1</figureCitation>
<figureCitationid="3CCB15619416D4409F12433015D33E03"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">, 2</figureCitation>
<figureCitationid="7DEBAE0CEA11CBA4F9167BCE9AE7CA2C"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">, 3</figureCitation>
<paragraphid="3AAE0D7DCB5CA8DD8D39A0F9140CD20D"pageId="0"pageNumber="135">Body length of mature individuals reaching ~15 cm. Somite VII quinquannulate, somites VIII-XXV sexannulate, b1 = b2 <a2 = b5> c11 = c12 (especially mid-body somites). Male gonopore in slightly anterior to middle of, or in middle of somite XI c12, female gonopore in somite XIII b2/a2, posterior to gastropore, gonopores separated by 1/2 + 8 annuli. Pharynx reaching to somite XIV a2/b5-b5. Gastropore conspicuous, in somite XIII b2/a2. Gastroporal duct thick tubular. Paired epididymides in somites XVI-XVIII, occupying 11-12 annuli. Atrial cornua developed, ovate, without pre-atrial loop. Dorsal surface reddish brown in life.</paragraph>
<figureCitationid="46DCDABF4692406FD0EBF3723D0AFEAB"captionStart="Figure 1"captionStartId="F1"captionText="Figure 1. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view; B. Ventral view; C. Dorsal view of live animal. Scale bars: 20 mm (same bar for A, B)."httpUri="https://binary.pensoft.net/fig/714572"pageId="0"pageNumber="135">1A, B</figureCitation>
<figureCitationid="24D8421114BC79E2BB1C139120193C6A"captionStart="Figure 1"captionStartId="F1"captionText="Figure 1. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view; B. Ventral view; C. Dorsal view of live animal. Scale bars: 20 mm (same bar for A, B)."httpUri="https://binary.pensoft.net/fig/714572"pageId="0"pageNumber="135">1B</figureCitation>
<figureCitationid="2AFE2359C85389A95E89B70B4C1678FF"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2C, D</figureCitation>
<figureCitationid="F4CBE08FE04120A4B451EE91A8CDAEE7"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A</figureCitation>
<figureCitationid="EABDADDE7EA2EBBFCCF119125FE4150C"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A</figureCitation>
<figureCitationid="3E0326FA16730FC688157E3110667DE6"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A, B</figureCitation>
<figureCitationid="C5FE17E3F383CECE9012E3118DF97B80"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A, B</figureCitation>
<figureCitationid="3ED59054305CDF6E8C913F5FDAF57926"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A, B</figureCitation>
<figureCitationid="ED4295503097FFE8F31773941C37544E"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A-E</figureCitation>
<figureCitationid="0F9FE4F021EC5764F5F20FA3A11B9C6B"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2E</figureCitation>
<figureCitationid="8444E246E13472E712427C04B68A306A"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2C, D</figureCitation>
<figureCitationid="28A5D9884DDFEFFF4D41556C3D2538F6"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2C</figureCitation>
<figureCitationid="2F134FD33345776D58E9AF2EA1E71F9D"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2F</figureCitation>
<figureCitationid="BDD5C8F481649F901DD57E98CD4620B2"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2F, G</figureCitation>
<figureCitationid="5804446D034EDD280D56AC3FCC639CA0"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2F</figureCitation>
Anterior ganglionic mass in somite VI a2 and a3. Ganglion VII in somite VII b2. Ganglia VIII and IX, of each somite, in b2 and a2. Ganglia X and XI, of each somite, in a2 (Fig.
<figureCitationid="E81153F6DF9BE27217ADF61123FAE1DB"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
<figureCitationid="B5ED671C0A76A1E1B107A9D4D3D6FB4D"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
<figureCitationid="B4F6397F26904BBB2582491BA66EF5F3"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
<figureCitationid="C6875CAC3ABE33CBC5467E74D9C5EAA5"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
<figureCitationid="1A269BD99D390ABE9970913B66FFB731"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
<figureCitationid="BF33D3E843D4ED15FD8C9B6764E2837D"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
). Ganglia XXIII and XXIV, of each somite, in a2. Ganglion XXV in somite XXV b2 and a2. Ganglion XXVI in somite XXVI b1 and b2. Posterior ganglionic mass in somite XXVI c11 and c12.
<figureCitationid="AB38DB0CFFBB42C210116154C120D5B0"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2A</figureCitation>
), 2nd and 3rd pairs dorsolaterally on posterior margin of somite V (a1 + a2). Papillae numerous, minute, hardly visible, 1 row on dorsal surface of every annulus.
<figureCitationid="ED16FDA679B6BCFE1E330498930B4BF8"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2B, D, F</figureCitation>
<figureCitationid="76DE8BEE925E4F2A24D6F49BECA70828"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3A</figureCitation>
<figureCitationid="64FF4EB1C82EEF4B6C1DE42C5E01A516"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view of somites I-VIII; B. Ventral view of somites I-VIII; C. Dorsal view of somites XXIV-XXVII and caudal sucker; D. Ventral view of somites XXIV-XXVII and caudal sucker; E. Dorsal view of somite XVI; F. Ventral view of somites XI-XIII; G. Ventral view of gastropore and female gonopore. Scale bars: 2.5 mm (A, B, E); 5 mm (C, D, F); 0.5 mm (G)."httpUri="https://binary.pensoft.net/fig/714573"pageId="0"pageNumber="135">2F, G</figureCitation>
). Gastroporal duct thick, tubular, slightly winding at junction with gastropore, reaching to somite XIV a2 and connecting with anteriormost part of crop (Fig.
<figureCitationid="4AB5910E1BEB719038E97EEAC5251F59"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3A</figureCitation>
<figureCitationid="FEC905913D24A8AEAD35B120941395ED"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
); on right side, in total ~83 testisacs, 13 in XIX, 14 in XX, 13 in XXI, 11 in XXII, 12 in XXIII, 11 in XXIV, 9 in XXV; on left side, in total ~80 testisacs, 12 in XIX, 12 in XX, 13 in XXI, 14 in XXII, 11 in XXIII, 10 in XXIV, 8 in XXV. Paired epididymides; right epididymis in somite XVII b1 to somite XVIII/XIX, occupying 12 annuli; left epididymis in somite XVII b1/b2 to somite XVIII/XIX, occupying 11 annuli (Fig.
<figureCitationid="874CDFF618712C3D4FC532BAC9A66D31"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B</figureCitation>
). Paired ejaculatory ducts; right duct in somite XI b5 to somite XVII b1; left duct in somite XI b5 to somite XVII b1/b2; coiled in position posterior to ovisacs; each duct crossing ventrally beneath each ovisac, then nearly straight in position anterior to ovisacs; each widening from respective junction with epididymis, narrowing at junction with atrial cornua, then turning proximally toward atrial cornua without pre-atrial loop (Fig.
<figureCitationid="ECEC918EF463ADDFE0357C7B70A84300"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B, C</figureCitation>
<figureCitationid="1DF6993D1DE0E4DA027D046ACE75068D"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B-E</figureCitation>
<figureCitationid="BB2066AE9EA54C867E300B7DE26CAC98"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3C-E</figureCitation>
<figureCitationid="621AF31FAFBB1CB8AC781AC36026959E"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B, F</figureCitation>
<figureCitationid="C20D8261A9F5112D8EEDDB224FD3DB44"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3B, F</figureCitation>
<figureCitationid="16B56E650883846ED2EA65F8BA2F3696"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Ventral view of gastroporal duct; B. Dorsal view of reproductive system, including ventral nervous system; C. Dorsal view of male atrium including positions of ganglia XI and XII; D. Left lateral view of male atrium; E. Ventral view of male atrium; F. Dorsal view of female reproductive system, including position of ganglion XIII. Scale bars: 5 mm (A); 10 mm (B); 2 mm (C, E); 1 mm (D, F)."httpUri="https://binary.pensoft.net/fig/714574"pageId="0"pageNumber="135">3F</figureCitation>
<emphasisid="BE6E885F35E53493E8B37CC91969D634"bold="true"pageId="0"pageNumber="135">(based on the holotype KUZ Z4031 and paratype KUZ Z4032).</emphasis>
<figureCitationid="68B5E8CB1573058904278B839059640D"captionStart="Figure 1"captionStartId="F1"captionText="Figure 1. Orobdella ibukifukuyamai sp. nov., holotype, KUZ Z 4031. A. Dorsal view; B. Ventral view; C. Dorsal view of live animal. Scale bars: 20 mm (same bar for A, B)."httpUri="https://binary.pensoft.net/fig/714572"pageId="0"pageNumber="135">1C</figureCitation>
); lateral surface ocher; ventral surface whitish yellow. Color faded in preservative; dark mid-dorsal line present from somite VII b6 to somite XXVII only in paratype.
Measurements: BL 121.1 mm, BW 6.3 mm, CL 3.3 mm, CW 4.1 mm. Somites XVI-XXI sexannulate, b1 = b2 <a2 (b3 = b4 dorsally) = b5 (c9 = c10 dorsally)> c11> c12. Pharynx reaching to somite XIV b5. Crop reaching to somite XXI/XXII. Gastroporal duct reaching to somite XIV a2/b5. Intestine reaching to somite XXV b1. Paired epididymides in somite XVI b2/a2 to somite XVIII b1/b2, occupying 11 annuli. Paired ejaculatory ducts in somite XI b5 to somite XVI b2/a2; coiled in position anterior to ovisacs. Paired ovisacs; right ovisac in somite XIII a2-c11; left ovisac in somite XIII a2 and b5. Right oviduct crossing ventrally beneath nerve cord.
<paragraphid="37CBD83701530D381AC69A795BD9706D"pageId="0"pageNumber="135">The specific name is dedicated to Mr Ibuki Fukuyama who collected the specimens of the new species.</paragraph>
<figureCitationid="A657AB0C0AD1DC78759B49725BCB591A"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. Bayesian inference tree for 8090 bp of nuclear 18 S rRNA, 28 S rRNA and H 3, and mitochondrial COI, tRNACys, tRNAMet, 12 S rRNA, tRNAVal, 16 S rRNA, tRNALeu and ND 1 markers. Numbers on nodes indicate bootstrap (BS) values for maximum likelihood ≥ 60 % and Bayesian posterior probabilities (PP) ≥ 0.90. Double asterisks denote the node with BS = 100 % and PP = 1.0; single asterisk denotes the node with BS ≥ 80 %, PP ≥ 0.95. Numbers in parentheses represent the mid-body somite annulation of each species. The geographical range of each group / lineage within Orobdella is also indicated."httpUri="https://binary.pensoft.net/fig/714575"pageId="0"pageNumber="135">4</figureCitation>
<bibRefCitationid="44C6023729C279D246CED5B34829FF2E"DOI="https://doi.org/10.25674/so93iss2id154"author="Nakano, T"journalOrPublisher="Soil Organisms"pageId="0"pageNumber="135"pagination="105 - 113"refId="B12"refString="Nakano, T, 2021. A new species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from the Tsukuba Mountains in Japan. Soil Organisms 93: 105 - 113, DOI: https://doi.org/10.25674/so93iss2id154"title="A new species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from the Tsukuba Mountains in Japan."url="https://doi.org/10.25674/so93iss2id154"volume="93"year="2021">Nakano 2021</bibRefCitation>
Bayesian inference tree for 8090 bp of nuclear 18S rRNA, 28S rRNA and H3, and mitochondrial COI, tRNACys, tRNAMet, 12S rRNA, tRNAVal, 16S rRNA, tRNALeu and ND1 markers. Numbers on nodes indicate bootstrap (BS) values for maximum likelihood ≥ 60% and Bayesian posterior probabilities (PP) ≥ 0.90. Double asterisks denote the node with BS = 100% and PP = 1.0; single asterisk denotes the node with BS ≥ 80%, PP ≥ 0.95. Numbers in parentheses represent the mid-body somite annulation of each species. The geographical range of each group/lineage within
<tableCitationid="7DAAC68F1AAF9A73DECAF7163BD7CC08"captionStart="Table 1"captionStartId="T1"captionText="Table 1. Uncorrected p - distances for the 1267 bp COI sequences of O. ibukifukuyamai sp. nov. and the closely related sexannulate species, O. okanoi and O. yamaneae. Acronym: KUZ, Zoological Collection of Kyoto University."httpUri="http://table.plazi.org/id/D771932DEBA9B54B09299216A08B71FF"pageId="0"pageNumber="135"tableUuid="D771932DEBA9B54B09299216A08B71FF">1</tableCitation>
<bibRefCitationid="872EE45303A95EEE09B9A2C15F022E27"DOI="https://doi.org/10.3897/zse.91.7616"author="Nakano, T"journalOrPublisher="Zoosystematics and Evolution"pageId="0"pageNumber="135"pagination="79 - 102"refId="B9"refString="Nakano, T, 2016. Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae). Zoosystematics and Evolution 92: 79 - 102, DOI: https://doi.org/10.3897/zse.91.7616"title="Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae)."url="https://doi.org/10.3897/zse.91.7616"volume="92"year="2016">Nakano 2016</bibRefCitation>
. Only two specimens of the new species could be examined in this study, but nonetheless, their morphological characteristics and DNA sequences successfully ensured the distinct species status of
<bibRefCitationid="F5FB7BB7125898F7B60AB783C3CC4BA5"DOI="https://doi.org/10.11646/zootaxa.2998.1.1"author="Nakano, T"journalOrPublisher="Zootaxa"pageId="0"pageNumber="135"pagination="1 - 15"refId="B7"refString="Nakano, T, 2011. Redescription of Orobdella ijimai (Hirudinida: Arhynchobdellida: Gastrostomobdellidae), and two new species of Orobdella from the Ryukyu Archipelago, Japan. Zootaxa 2998: 1 - 15, DOI: https://doi.org/10.11646/zootaxa.2998.1.1"title="Redescription of Orobdella ijimai (Hirudinida: Arhynchobdellida: Gastrostomobdellidae), and two new species of Orobdella from the Ryukyu Archipelago, Japan."url="https://doi.org/10.11646/zootaxa.2998.1.1"volume="2998"year="2011">Nakano 2011</bibRefCitation>
<bibRefCitationid="EFF946DACF9B494E0FE41DB75FC12672"DOI="https://doi.org/10.3897/zookeys.181.2932"author="Nakano, T"journalOrPublisher="ZooKeys"pageId="0"pageNumber="135"pagination="79 - 93"refId="B8"refString="Nakano, T, 2012. A new sexannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from Yakushima Island, Japan. ZooKeys 181: 79 - 93, DOI: https://doi.org/10.3897/zookeys.181.2932"title="A new sexannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from Yakushima Island, Japan."url="https://doi.org/10.3897/zookeys.181.2932"volume="181"year="2012">2012</bibRefCitation>
<bibRefCitationid="2B15E0F53989C2020141365A3718536D"DOI="https://doi.org/10.3897/zse.91.7616"author="Nakano, T"journalOrPublisher="Zoosystematics and Evolution"pageId="0"pageNumber="135"pagination="79 - 102"refId="B9"refString="Nakano, T, 2016. Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae). Zoosystematics and Evolution 92: 79 - 102, DOI: https://doi.org/10.3897/zse.91.7616"title="Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae)."url="https://doi.org/10.3897/zse.91.7616"volume="92"year="2016">2016</bibRefCitation>
<tableCitationid="3A1607DF08B13F31F3CADAEE920493F1"captionStart="Table 2"captionStartId="T2"captionText="Table 2. Comparisons of morphological characters between Orobdella ibukifukuyamai sp. nov. and six sexannulate congeners plus the octannulate O. nakahamai."httpUri="http://table.plazi.org/id/7A9CE034F681B9ACD33F0BF888C0ACF8"pageId="0"pageNumber="135"tableUuid="7A9CE034F681B9ACD33F0BF888C0ACF8">2</tableCitation>
shares several morphological features, i.e., a large body size reaching ~15 cm, a similar annulation pattern of somites VII and VIII, and a similar position of epididymides. However,
by the reddish brown dorsal surface and ocher lateral surface, the possession of 1/2 + 8 annuli between the gonopores, and the ovate atrial cornua without pre-atrial loops (in
<tableCitationid="FA0589B9E8CF083E92451418CF3E6635"captionStart="Table 2"captionStartId="T2"captionText="Table 2. Comparisons of morphological characters between Orobdella ibukifukuyamai sp. nov. and six sexannulate congeners plus the octannulate O. nakahamai."httpUri="http://table.plazi.org/id/7A9CE034F681B9ACD33F0BF888C0ACF8"pageId="0"pageNumber="135"tableUuid="7A9CE034F681B9ACD33F0BF888C0ACF8">2</tableCitation>
<tdid="8E94B3F873C0CDBBEA513D1A867ED48F"colspan="1"pageId="0"pageNumber="135"rowspan="1">anterior and posterior parts grayish purple, mid-body amber</td>
<bibRefCitationid="B20520A06CDF249E4CB043C120CB50ED"DOI="https://doi.org/10.2108/zs160167"author="Nakano, T"journalOrPublisher="Zoological Science"pageId="0"pageNumber="135"pagination="161 - 172"refId="B11"refString="Nakano, T, 2017b. A new species of Orobdella (Hirudinida: Arhynchobdellida: Orobdellidae) from Japan reveals the function of the Orobdella gastroporal duct. Zoological Science 34: 161 - 172, DOI: https://doi.org/10.2108/zs160167"title="A new species of Orobdella (Hirudinida: Arhynchobdellida: Orobdellidae) from Japan reveals the function of the Orobdella gastroporal duct."url="https://doi.org/10.2108/zs160167"volume="34"year="2017 b">Nakano 2017b</bibRefCitation>
<bibRefCitationid="2B8AE3DAA9AA687F85A23AD1F544C4A7"DOI="https://doi.org/10.25674/so93iss2id154"author="Nakano, T"journalOrPublisher="Soil Organisms"pageId="0"pageNumber="135"pagination="105 - 113"refId="B12"refString="Nakano, T, 2021. A new species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from the Tsukuba Mountains in Japan. Soil Organisms 93: 105 - 113, DOI: https://doi.org/10.25674/so93iss2id154"title="A new species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from the Tsukuba Mountains in Japan."url="https://doi.org/10.25674/so93iss2id154"volume="93"year="2021">Nakano 2021</bibRefCitation>
bears the sexannulate mid-body somites, of which annuli a2 and b5 are, respectively, subdivided dorsally, defined as b1 = b2 <a2 (b3 = b4 dorsally) = b5 (c9 = c10 dorsally)> c11 = c12, the new species may exhibit annulation that is the transitional state (= secondarily octannulate dorsally) between sexannulate and octannulate
