<documentID-CLB-Dataset="24201"ID-DOI="http://dx.doi.org/10.3897/mycokeys.62.39699"ID-GBIF-Dataset="cbd9d6e5-d75b-4155-8c1b-b14717d0aea7"ID-PMC="PMC7010843"ID-Pensoft-Pub="1314-4049-62-53"ID-Pensoft-UUID="6ECEEE5E152356598937B06C4AC0B2CC"ID-PubMed="32076382"ModsDocID="1314-4049-62-53"checkinTime="1580810321805"checkinUser="pensoft"docAuthor="Sulzbacher, Marcelo A., Orihara, Takamichi, Grebenc, Tine, Wartchow, Felipe, Smith, Matthew E., Martin, Maria P., Giachini, Admir J. & Baseia, Iuri G."docDate="2020"docId="A0845E6B1CDF5CB3A7D1A0F4785D6D50"docLanguage="en"docName="MycoKeys 62: 53-73"docOrigin="MycoKeys 62"docSource="http://dx.doi.org/10.3897/mycokeys.62.39699"docTitle="Longistriata flava Sulzbacher, Orihara, Grebenc, M. P. Martin & Baseia 2020, sp. nov."docType="treatment"docVersion="7"id="6ECEEE5E152356598937B06C4AC0B2CC"lastPageNumber="53"masterDocId="6ECEEE5E152356598937B06C4AC0B2CC"masterDocTitle="Longistriata flava (Boletaceae, Basidiomycota) - a new monotypic sequestrate genus and species from Brazilian Atlantic Forest"masterLastPageNumber="73"masterPageNumber="53"pageNumber="53"updateTime="1719971504746"updateUser="ExternalLinkService">
<mods:titleid="28EBBE2C9A81CB14D63717566FAE7853">Longistriata flava (Boletaceae, Basidiomycota) - a new monotypic sequestrate genus and species from Brazilian Atlantic Forest</mods:title>
<taxonomicNameid="728CB5348E516722FE54535E24386B14"ID-CoL="B2MS6"LSID="A0845E6B-1CDF-5CB3-A7D1-A0F4785D6D50"authority="Sulzbacher, Orihara, Grebenc, M. P. Martín & Baseia"authorityName="Sulzbacher, Orihara, Grebenc, M. P. Martin & Baseia"authorityYear="2020"class="Agaricomycetes"family="Boletaceae"genus="Longistriata"higherTaxonomySource="CoL"kingdom="Fungi"lsidName="Longistriata flava"order="Boletales"pageId="0"pageNumber="53"phylum="Basidiomycota"rank="species"species="flava"status="sp. nov.">
<figureCitationid="CD30D4E30B1B0B00F439F8D49C2C2B35"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. A-G Longistriata flava (UFRN-Fungus 1756, holotype) A-B fresh mature basidioma C hymenophoral trama mounted in 3 % KOH with Congo Red D interwoven hyphae of peridium (upper left) and hymenophoral trama mounted in 3 % KOH with Congo Red E hymenial cystidia mounted in 3 % KOH with Congo Red F basidiospores mounted in Melzer's reagent G basidiospores mounted in 3 % KOH."figureDoi="10.3897/mycokeys.62.39699.figure3"httpUri="https://binary.pensoft.net/fig/375566"pageId="0"pageNumber="53">Figs 3</figureCitation>
<figureCitationid="9DC4F05C70CFC2182264A3A1E6310B90"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. A-D Longistriata flava (UFRN-Fungus 1756, holotype) A hymenial cystidia B basidioles and basidium C details of the peridium with interwoven hyphae D polar and longitudinal view of basidiospores."figureDoi="10.3897/mycokeys.62.39699.figure4"httpUri="https://binary.pensoft.net/fig/375567"pageId="0"pageNumber="53">, 4</figureCitation>
<figureCitationid="878DDDE50E571B0FE097E14A6680D6CA"captionStart="Figure 5"captionStartId="F5"captionText="Figure 5. A-D Basidiospores of Longistriata flava (UFRN-Fungus 1756, holotype) as observed with scanning electron microscopy. Note the persistent sterigmal attachment and a series of thinner longitudinal ridges (on average> 10 complete ridges across the longitudinal axis of the spore) with additional irregular, thin, low and bifurcated or fused ridges."figureDoi="10.3897/mycokeys.62.39699.figure5"httpUri="https://binary.pensoft.net/fig/375568"pageId="0"pageNumber="53">, 5</figureCitation>
Basidiomata hypogeous to subhypogeous, 11-24 mm wide, 13-16 mm high; subglobose, depressed subglobose to oblong in older stages, with small folds at the base; with a short stipe (Fig.
<figureCitationid="6B53C4B2BA29A059B75BA867C4F7E7D9"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. A-G Longistriata flava (UFRN-Fungus 1756, holotype) A-B fresh mature basidioma C hymenophoral trama mounted in 3 % KOH with Congo Red D interwoven hyphae of peridium (upper left) and hymenophoral trama mounted in 3 % KOH with Congo Red E hymenial cystidia mounted in 3 % KOH with Congo Red F basidiospores mounted in Melzer's reagent G basidiospores mounted in 3 % KOH."figureDoi="10.3897/mycokeys.62.39699.figure3"httpUri="https://binary.pensoft.net/fig/375566"pageId="0"pageNumber="53">3A-B</figureCitation>
). Peridium <0.8 mm thick, at younger stages yellow (2A6) to light yellow (1A5) then yellowish brown (5D8) to brownish yellow (5C8) at maturity; smooth and glabrous, sometimes finely fibrillose. Sterile base present, short, 6-8
3-4 mm, clavate with a bulbous slightly developed base; color vivid yellow (3A8), brownish yellow (5C8) when bruised; surface glabrous, with small folds and depressions; the inner part is full, subgelatinous and yellowish brown (5D8); connected by scattered and short, thin (0.3-0.5 mm diam), orange (6B8) rhizomorphs. Gleba loculate, non-gelatinized to gelatinized, with irregular locules (0.5-1 mm diam); white (1A1) at younger stages, to finally yellowish brown (5F4) at maturity, immediately turning deep green (30F7) to black when cut in older basidiomata.
<taxonomicNameid="BABECAA3491B09AEBB45CE67B81A4DF3"authorityName="Sulzbacher, Orihara, Grebenc, M. P. Martin & Baseia"authorityYear="2020"class="Chromadorea"family="Trichostrongylidae"genus="Longistriata"higherTaxonomySource="CoL"kingdom="Animalia"lsidName="Longistriata flava"order="Rhabditida"pageId="0"pageNumber="53"phylum="Nematoda"rank="species"species="flava">
<figureCitationid="881B43A801BAD8E6E37744D3A748F201"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. A-D Longistriata flava (UFRN-Fungus 1756, holotype) A hymenial cystidia B basidioles and basidium C details of the peridium with interwoven hyphae D polar and longitudinal view of basidiospores."figureDoi="10.3897/mycokeys.62.39699.figure4"httpUri="https://binary.pensoft.net/fig/375567"pageId="0"pageNumber="53">4C</figureCitation>
diam., with rounded, thin-walled, smooth, terminal hyphae, not readily separable from gleba. Hymenophoral trama formed by parallel to subparallel, smooth and thin-walled, hyaline hyphae, inamyloid, gelatinized in the central part, 3-6
<figureCitationid="55D5C7577D779A5AF0BC9EB5F6241105"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. A-G Longistriata flava (UFRN-Fungus 1756, holotype) A-B fresh mature basidioma C hymenophoral trama mounted in 3 % KOH with Congo Red D interwoven hyphae of peridium (upper left) and hymenophoral trama mounted in 3 % KOH with Congo Red E hymenial cystidia mounted in 3 % KOH with Congo Red F basidiospores mounted in Melzer's reagent G basidiospores mounted in 3 % KOH."figureDoi="10.3897/mycokeys.62.39699.figure3"httpUri="https://binary.pensoft.net/fig/375566"pageId="0"pageNumber="53">3C-D</figureCitation>
<figureCitationid="2F27BE08730BA83E5A06EAE1C1CFE41E"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. A-G Longistriata flava (UFRN-Fungus 1756, holotype) A-B fresh mature basidioma C hymenophoral trama mounted in 3 % KOH with Congo Red D interwoven hyphae of peridium (upper left) and hymenophoral trama mounted in 3 % KOH with Congo Red E hymenial cystidia mounted in 3 % KOH with Congo Red F basidiospores mounted in Melzer's reagent G basidiospores mounted in 3 % KOH."figureDoi="10.3897/mycokeys.62.39699.figure3"httpUri="https://binary.pensoft.net/fig/375566"pageId="0"pageNumber="53">3E</figureCitation>
<figureCitationid="35B98A99B1617C9A69390CEB93F8ED33"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. A-D Longistriata flava (UFRN-Fungus 1756, holotype) A hymenial cystidia B basidioles and basidium C details of the peridium with interwoven hyphae D polar and longitudinal view of basidiospores."figureDoi="10.3897/mycokeys.62.39699.figure4"httpUri="https://binary.pensoft.net/fig/375567"pageId="0"pageNumber="53">4A</figureCitation>
<figureCitationid="D3B30C4A626B6102C84F2EF34A1C3CB6"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. A-D Longistriata flava (UFRN-Fungus 1756, holotype) A hymenial cystidia B basidioles and basidium C details of the peridium with interwoven hyphae D polar and longitudinal view of basidiospores."figureDoi="10.3897/mycokeys.62.39699.figure4"httpUri="https://binary.pensoft.net/fig/375567"pageId="0"pageNumber="53">4B</figureCitation>
width); with a series of thinner longitudinal ridges, in average> 10 complete ridges across the longitudinal axis of the spore with additional irregular, thin and low ridges that are sometimes bifurcated (Figs
<figureCitationid="BE736FA4ACE0100E587E8A362DCC3F24"captionStart="Figure 3"captionStartId="F3"captionText="Figure 3. A-G Longistriata flava (UFRN-Fungus 1756, holotype) A-B fresh mature basidioma C hymenophoral trama mounted in 3 % KOH with Congo Red D interwoven hyphae of peridium (upper left) and hymenophoral trama mounted in 3 % KOH with Congo Red E hymenial cystidia mounted in 3 % KOH with Congo Red F basidiospores mounted in Melzer's reagent G basidiospores mounted in 3 % KOH."figureDoi="10.3897/mycokeys.62.39699.figure3"httpUri="https://binary.pensoft.net/fig/375566"pageId="0"pageNumber="53">3F-G</figureCitation>
<figureCitationid="F12A6B80EDC48875F9344D13EA43A05E"captionStart="Figure 4"captionStartId="F4"captionText="Figure 4. A-D Longistriata flava (UFRN-Fungus 1756, holotype) A hymenial cystidia B basidioles and basidium C details of the peridium with interwoven hyphae D polar and longitudinal view of basidiospores."figureDoi="10.3897/mycokeys.62.39699.figure4"httpUri="https://binary.pensoft.net/fig/375567"pageId="0"pageNumber="53">4D</figureCitation>
<figureCitationid="F99070D1CF7B44D5C2C89E39F79F6616"captionStart="Figure 5"captionStartId="F5"captionText="Figure 5. A-D Basidiospores of Longistriata flava (UFRN-Fungus 1756, holotype) as observed with scanning electron microscopy. Note the persistent sterigmal attachment and a series of thinner longitudinal ridges (on average> 10 complete ridges across the longitudinal axis of the spore) with additional irregular, thin, low and bifurcated or fused ridges."figureDoi="10.3897/mycokeys.62.39699.figure5"httpUri="https://binary.pensoft.net/fig/375568"pageId="0"pageNumber="53">5C</figureCitation>
<figureCitationid="3E35B825B37CA97619F9252BF776A49F"captionStart="Figure 5"captionStartId="F5"captionText="Figure 5. A-D Basidiospores of Longistriata flava (UFRN-Fungus 1756, holotype) as observed with scanning electron microscopy. Note the persistent sterigmal attachment and a series of thinner longitudinal ridges (on average> 10 complete ridges across the longitudinal axis of the spore) with additional irregular, thin, low and bifurcated or fused ridges."figureDoi="10.3897/mycokeys.62.39699.figure5"httpUri="https://binary.pensoft.net/fig/375568"pageId="0"pageNumber="53">5A-D</figureCitation>
<taxonomicNameid="6BA9CD2F2F65A5F75BA3D35020D3684B"authorityName="Sulzbacher, Orihara, Grebenc, M. P. Martin & Baseia"authorityYear="2020"class="Chromadorea"family="Trichostrongylidae"genus="Longistriata"higherTaxonomySource="CoL"kingdom="Animalia"lsidName="Longistriata flava"order="Rhabditida"pageId="0"pageNumber="53"phylum="Nematoda"rank="species"species="flava">
<taxonomicNameid="34446D9BA414C57B03A75FC48F3E15CE"authorityName="Sulzbacher, Orihara, Grebenc, M. P. Martin & Baseia"authorityYear="2020"class="Chromadorea"family="Trichostrongylidae"genus="Longistriata"higherTaxonomySource="CoL"kingdom="Animalia"lsidName="Longistriata flava"order="Rhabditida"pageId="0"pageNumber="53"phylum="Nematoda"rank="species"species="flava">
(UFRN-Fungus 1756, holotype) as observed with scanning electron microscopy. Note the persistent sterigmal attachment and a series of thinner longitudinal ridges (on average> 10 complete ridges across the longitudinal axis of the spore) with additional irregular, thin, low and bifurcated or fused ridges.
<bibRefCitationid="75F182D7264B0E6A3C1C0C32D9844AC1"DOI="https://doi.org/10.1038/ismej.2009.131"author="Tedersoo, L"journalOrPublisher="The ISME journal"pageId="0"pageNumber="53"pagination="1 - 465"refId="B68"refString="Tedersoo, L, Sadam, A, Zambrano, M, Valencia, R, Bahram, M, 2010b. Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot. The ISME journal 4 (4): 1 - 465, DOI: https://doi.org/10.1038/ismej.2009.131"title="Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot."url="https://doi.org/10.1038/ismej.2009.131"volume="4"year="2010 b">Tedersoo et al. 2010b</bibRefCitation>
). All known specimens were found in silicate silt to sandy soils, with moderately low pH (4.5-5.5), low available nutrients and low water capacity. Despite the close vicinity of the ocean, the absence of halophilic vegetation indicates a lack of salinification or accumulation of NaCl in soils.
The specimens UFRN-fungos 1756 and UFRN-fungos 2110 are sequestrate to emergent basidiomes that fruit in small groups. The basidiomes have a smooth and vivid yellow peridium that becomes dark green when exposed to air. They also have a central sterile base that is attached to short orange rhizomorphs, a white gleba formed of distinct locules that turns dark green to black when cut and hyaline to light brown, broadly ellipsoid basidiospores covered with a series of thin, dextrinoid longitudinal striations and ridges. These ridges and striations are sometimes bifurcated or irregular and they also cover the entire spore surface. The clavate basidia can be either 2-spored and 4-spored and the lageniform to ventricose cystidia are a notable feature in the hymenium. This combination of morphological features is unique within the
