179 lines
24 KiB
XML
179 lines
24 KiB
XML
|
<document id="B85BB74B039098AB45D6853C138BCF25" ID-DOI="10.1016/j.phytochem.2018.05.015" ID-ISSN="1873-3700" ID-Zenodo-Dep="10483880" IM.bibliography_approvedBy="juliana" IM.illustrations_approvedBy="juliana" IM.materialsCitations_approvedBy="juliana" IM.metadata_approvedBy="juliana" IM.tables_approvedBy="juliana" IM.taxonomicNames_approvedBy="juliana" IM.treatments_approvedBy="juliana" checkinTime="1704937900439" checkinUser="felipe" docAuthor="Maciel, Elisabete, Lillebø, Ana, Domingues, Pedro, Costa, Elisabete da, Calado, Ricardo & Domingues, M. Rosário M." docDate="2018" docId="03A487FFFFB3EC15047CEE4DCAD5FBA1" docLanguage="en" docName="Phytochemistry.153.94-101.pdf" docOrigin="Phytochemistry 153" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.05.015" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Halimione portulacoides" docType="treatment" docVersion="3" lastPageNumber="97" masterDocId="FF9DFF87FFB0EC160418ED70CE5CFFD2" masterDocTitle="Polar lipidome profiling of Salicornia ramosissima and Halimione portulacoides and the relevance of lipidomics for the valorization of halophytes" masterLastPageNumber="101" masterPageNumber="94" pageNumber="97" updateTime="1705326886214" updateUser="juliana">
|
|||
|
|
<mods:mods id="B2567AFD959E7C6C32D1914CC9D9B5DF" xmlns:mods="http://www.loc.gov/mods/v3">
|
|||
|
|
<mods:titleInfo id="92F24343B3A4D943B19A5F4DCDEE0B9E">
|
|||
|
|
<mods:title id="307DC5CC32E278B615ABFBC8994B6432">Polar lipidome profiling of Salicornia ramosissima and Halimione portulacoides and the relevance of lipidomics for the valorization of halophytes</mods:title>
|
|||
|
|
</mods:titleInfo>
|
|||
|
|
<mods:name id="922648EA736FF876E4B72E22F94469F4" type="personal">
|
|||
|
|
<mods:role id="A51F385FD373EE2EAD5ED86BB5302C32">
|
|||
|
|
<mods:roleTerm id="5D45016159C607436481D161D67CDBDB">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="68A09FABEC95B97553AD17464F73A105">Maciel, Elisabete</mods:namePart>
|
|||
|
|
<mods:affiliation id="7546D8DC9AE8FE9CBF4DC139D3B32D24">Centro de Espetrometria de Massa, Departamento de Química & QOPNA, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal & ∗ & Departamento de Biologia & CESAM & ECOMARE, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
<mods:nameIdentifier id="A97ADE74BA2EE307CF97442D538D8F14" type="email">elisabete.maciel@ua.pt</mods:nameIdentifier>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:name id="5613F234DB3D631FF36877C2B1DC9744" type="personal">
|
|||
|
|
<mods:role id="1B84B3B4E44E3263E8764689FA13CBA3">
|
|||
|
|
<mods:roleTerm id="B20FF16AEFF88AE3D09C1C82927C842F">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="45FD327FEB2A11C04021F5D8A4F34A30">Lillebø, Ana</mods:namePart>
|
|||
|
|
<mods:affiliation id="E0E4B2244EA1325FFD36672B3E9AFD89">Departamento de Biologia & CESAM & ECOMARE, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:name id="A9593052BA700F5B3D1917EA754A94AF" type="personal">
|
|||
|
|
<mods:role id="DA92CF16AC7C4D5A878FEE9037C58525">
|
|||
|
|
<mods:roleTerm id="35D62EDE050B89540DAC9DCA4BE40395">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="57C51F678ED940F62D1658CA644853DF">Domingues, Pedro</mods:namePart>
|
|||
|
|
<mods:affiliation id="CBD086404A3C51250D2E3460255952BA">Centro de Espetrometria de Massa, Departamento de Química & QOPNA, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:name id="F06C8F04CD4CF3D2BFE82191677C3BC3" type="personal">
|
|||
|
|
<mods:role id="BEDDD4B5A52753A714267DC502DC700D">
|
|||
|
|
<mods:roleTerm id="5199F6EA46BC613653E2EBCDF73EAA91">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="94ACF8B6C7B492B07C87884CD09CA11C">Costa, Elisabete da</mods:namePart>
|
|||
|
|
<mods:affiliation id="E39BDA654A639C5C1C389355BF91898B">Centro de Espetrometria de Massa, Departamento de Química & QOPNA, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:name id="78A51A96C1E3FD42E2EC583CFAD85C75" type="personal">
|
|||
|
|
<mods:role id="796B2E3D75AF70CB229973EAA559F7A0">
|
|||
|
|
<mods:roleTerm id="1D558A8C767AF34DEDDA9942A28F73D2">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="E24C8EE108000CC040CA9A5B79421AE3">Calado, Ricardo</mods:namePart>
|
|||
|
|
<mods:affiliation id="0490782EDE19820CA3FECC97A2D76C1E">Departamento de Biologia & CESAM & ECOMARE, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:name id="76BF8A7AED5062B705D1D4EA6BC4865B" type="personal">
|
|||
|
|
<mods:role id="6768C86B79B6A5E91A7B88D4D7D42053">
|
|||
|
|
<mods:roleTerm id="F9AFE40000C32EF86F9AD7D9D354F128">Author</mods:roleTerm>
|
|||
|
|
</mods:role>
|
|||
|
|
<mods:namePart id="36DC8EEC95771F04842FA083585A0C20">Domingues, M. Rosário M.</mods:namePart>
|
|||
|
|
<mods:affiliation id="66E7F432480FCE4AA229EB3415B23BF8">Centro de Espetrometria de Massa, Departamento de Química & QOPNA, Universidade de Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal</mods:affiliation>
|
|||
|
|
</mods:name>
|
|||
|
|
<mods:typeOfResource id="EE4EEFF33CF6A543EB09445B932DDB6E">text</mods:typeOfResource>
|
|||
|
|
<mods:relatedItem id="87CB8D9528D552AA0545D6C9C55C01D0" type="host">
|
|||
|
|
<mods:titleInfo id="30BD6FAB01E388FC1C58763980E81BB5">
|
|||
|
|
<mods:title id="67520855D8D4831F71B7015644263130">Phytochemistry</mods:title>
|
|||
|
|
</mods:titleInfo>
|
|||
|
|
<mods:part id="69C6F3E8F60F0D2D912461C25B862C23">
|
|||
|
|
<mods:date id="FE4B5B288A07B3A056FBF7EE7BFB6A7F">2018</mods:date>
|
|||
|
|
<mods:detail id="EB66FD6179DB08525B8A6FC78C90C998" type="pubDate">
|
|||
|
|
<mods:number id="B0DE661D13A2A2100282D28D7EB420D8">2018-09-30</mods:number>
|
|||
|
|
</mods:detail>
|
|||
|
|
<mods:detail id="417FDD58C00298C1621A294FC2F1D37B" type="volume">
|
|||
|
|
<mods:number id="7656D46018F5C54771722AE31E25BA21">153</mods:number>
|
|||
|
|
</mods:detail>
|
|||
|
|
<mods:extent id="B424ECC027D8F9AA723E3F5E6169265A" unit="page">
|
|||
|
|
<mods:start id="C42D39F7EF36BB3855EB9A53CD3F2B7B">94</mods:start>
|
|||
|
|
<mods:end id="31A609C9CEDBC59CF29E4C6DB6ABA965">101</mods:end>
|
|||
|
|
</mods:extent>
|
|||
|
|
</mods:part>
|
|||
|
|
</mods:relatedItem>
|
|||
|
|
<mods:location id="955C526BC328C32438D5F4245B5FCF06">
|
|||
|
|
<mods:url id="1968E0EAFAB333AA3E8D2497B017EBBC">http://dx.doi.org/10.1016/j.phytochem.2018.05.015</mods:url>
|
|||
|
|
</mods:location>
|
|||
|
|
<mods:classification id="26F3856FBFAC70E6E126B0E75ABF6FF4">journal article</mods:classification>
|
|||
|
|
<mods:identifier id="A076C65E3C941C19F377C978F81FEE8E" type="DOI">10.1016/j.phytochem.2018.05.015</mods:identifier>
|
|||
|
|
<mods:identifier id="42EC166D3636064F69BB40134306C9FA" type="ISSN">1873-3700</mods:identifier>
|
|||
|
|
<mods:identifier id="C8DE25136F01D1AFB95856C2B14AF90E" type="Zenodo-Dep">10483880</mods:identifier>
|
|||
|
|
</mods:mods>
|
|||
|
|
<treatment id="03A487FFFFB3EC15047CEE4DCAD5FBA1" LSID="urn:lsid:plazi:treatment:03A487FFFFB3EC15047CEE4DCAD5FBA1" httpUri="http://treatment.plazi.org/id/03A487FFFFB3EC15047CEE4DCAD5FBA1" lastPageNumber="97" pageId="3" pageNumber="97">
|
|||
|
|
<subSubSection id="C3176562FFB3EC15047CEE4DCFA9FC82" box="[100,501,829,848]" pageId="3" pageNumber="97" type="nomenclature">
|
|||
|
|
<paragraph id="8BB236E9FFB3EC15047CEE4DCFA9FC82" blockId="3.[100,501,829,848]" box="[100,501,829,848]" pageId="3" pageNumber="97">
|
|||
|
|
<heading id="D0FA8185FFB3EC15047CEE4DCFA9FC82" bold="true" box="[100,501,829,848]" fontSize="36" level="1" pageId="3" pageNumber="97" reason="1">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15047CEE4DCFA9FC82" bold="true" box="[100,501,829,848]" italics="true" pageId="3" pageNumber="97">
|
|||
|
|
2.2. Lipid profile of
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC150506EE4DCFA9FC82" authority="(L.)" baseAuthorityName="L." box="[286,501,829,848]" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">Halimione portulacoides</taxonomicName>
|
|||
|
|
</emphasis>
|
|||
|
|
</heading>
|
|||
|
|
</paragraph>
|
|||
|
|
</subSubSection>
|
|||
|
|
<subSubSection id="C3176562FFB3EC15049DEE05CAD5FBA1" pageId="3" pageNumber="97" type="description">
|
|||
|
|
<paragraph id="8BB236E9FFB3EC15049DEE05CCB3FB99" blockId="3.[100,771,884,1992]" pageId="3" pageNumber="97">
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC15049DEE05CF03FC5A" box="[133,351,885,904]" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15049DEE05CF03FC5A" bold="true" box="[133,351,885,904]" italics="true" pageId="3" pageNumber="97">Halimione portulacoides</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
exhibited nineteen different FA (
|
|||
|
|
<tableCitation id="C68F0352FFB3EC1506B2EE05CCA8FC5A" box="[682,756,885,904]" captionStart="Table 1" captionStartId="1.[818,868,161,178]" captionTargetPageId="1" captionText="Table 1 Fatty acid composition of total lipids in leaves of two halophytes: Halimione portulacoides and Salicornia ramosissima (% of total FA)." pageId="3" pageNumber="97">Table 1</tableCitation>
|
|||
|
|
). The FA profile was characterized by a high percentage of PUFA (∼60%), in particular, omega-3 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505A0EEDCCF9FFC6D" bold="true" box="[440,451,940,959]" italics="true" pageId="3" pageNumber="97">n</emphasis>
|
|||
|
|
-3) FA (nearly 45%). The most abundant fatty acids contained 16 and 18 carbon atoms, and C18:3
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1506FCEEB8CCB3FC09" bold="true" box="[740,751,968,987]" italics="true" pageId="3" pageNumber="97">n</emphasis>
|
|||
|
|
-3 (43.5%), which was the main FA present in
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC150601EE94CCF0FC25" box="[537,684,996,1015]" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150601EE94CCF0FC25" bold="true" box="[537,684,996,1015]" italics="true" pageId="3" pageNumber="97">H. portulacoides</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
. The FA 16:0 was the second most abundant FA (19.2%), being the most represented SFA. MUFA accounted for ∼12% of total FA, with oleic acid C18:1
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150483E948CEF1FB99" bold="true" box="[155,173,1080,1099]" italics="true" pageId="3" pageNumber="97">n-</emphasis>
|
|||
|
|
9 being the most abundant (∼10% of the total pool of FAs).
|
|||
|
|
</paragraph>
|
|||
|
|
<paragraph id="8BB236E9FFB3EC15049DE924CC8FF8EE" blockId="3.[100,771,884,1992]" pageId="3" pageNumber="97">
|
|||
|
|
The LC− MS− based approach employed allowed the identification of 160 PL molecular species distributed over five classes: 69 PC, 43 PE, 20 PA, 21 PG and 7 PI molecular species; three glycolipid classes: SQDG, MGDG and DGDG, and one glycosphingolipid HexCer in the lipid extracts of
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC1504E3E9B3CFD7FB04" box="[251,395,1219,1238]" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1504E3E9B3CFD7FB04" bold="true" box="[251,395,1219,1238]" italics="true" pageId="3" pageNumber="97">H. portulacoides</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
. The distribution of acyl chains differed between PL classes, as shown in
|
|||
|
|
<tableCitation id="C68F0352FFB3EC150589E9AFCF8AFB20" box="[401,470,1247,1266]" captionStart="Table 4" captionStartId="4.[100,150,161,178]" captionTargetBox="[116,1471,226,1269]" captionTargetPageId="4" captionText="Table 4 Phospholipid molecular species identified by HILIC−Q−Exactive−MS from total lipid extract of Halimione portulacoides. [M + H]+ Phosphatidylcholine 685.378 PA 16:0/19:2; 17:0/18:2; 17:1/18:1 732.554 PC 16:0/16:1; 14:0/18:1 691.433 PA 18:3/18:3 734.569 PC 16:0/16:0 693.448 PA 18:2/18:3 756.556 PC 16:0/18:3; 16:1/18:2 695.464 PA 18:2/18:2; 18:1/18:3 758.571 PC 16:0/18:2,16:1/18:1 697.479 PA 18:1/18:2 760.585 PC 16:0/16:1 699.495 PA 18:1/18:1; 18:0/18:2 768.554 PC 17:1/18:3; 17:2/18:2; 17:3/18:1 701.504 PA 18:0/18:1 770.569 PC 17:0/18:3; 17:1/18:2; 17:2/18:1 [M − H]− Phosphatidylethanolamine 772.585 PC 17:0/18:2; 17:1/18:1; 17:2/18:0; 16:0/19:2 700.49 PE 15:0/18:2; 15:1/18:1; 17:1/16:1; 17:2/16:0 774.601 PC 17:0/18:1 712.489 PE 16:0/18:3; 16:1/18:2 778.538 PC 18:3/18:3 714.505 PE 16:0/18:2; 16:1/18:1 780.554 PC 18:2/18:3, 16:1/20:4 726.505 PE 17:1/18:2; 17:0/18:3; 16:0/19:3 782.566 PC 18:1/18:3; 18:2/18:2 728.520 PE 16:0/19:2; 17:0/18.2; 17:1/18:1 784.586 PC 18:1/18:2; 18:0/18:3 734.474 PE 18:3/18:3 786.601 PC 18:1/18:1, 18:0/18:2, 16:2/20:0 736.489 PE 18:2/18:3 788.606 PC 18:0/18:1, 16:1/20:0 738.505 PE 18:2/18:2; 18:1/18:3 794.569 PC 18:3/19:2 740.520 PE 18:1/18:2; 18:0/18:3 796.585 PC 18:2/19:2; 18:3/19:2 742.535 PE 18:0/18:2 798.601 PC 118:1/19:2; 18:2/19:1; 18:3/19:0 760.474 PE 16:0/21:0 800.522 PC 1 18:0/19:2; 18:1/19:1; 18:2/19:0 766.536 PE 18:3/20:1; 18:2/20:2; 18:1/20:3 802.538 PC 18:1/19:0 768.552 PE 18:3/20:0; 18:2/20:1; 18:1/20:2 804.552 PC 16:0/19:0 782.459 PE 18:3/21:0; 19:1/20:2; 18:2/21:1; 16:0/23:3 806.569 PC 16:0/22:6 784.474 PE 18:2/21:0; 19:0/20:2; 19:1/20:1 808.583 PC 18:3/20:2; 18:2/20:3 794.491 PE 18:2/22:2 810.596 PC 18:3/20:1; 18:2/20:2; 18:1/20:3 796.508 PE 18:3/22:0; 18:2/22:1 812.616 PC 18:3/20:0; 18:2/20:1; 18:1/20:2 798.522 PE 18:2/22:0; 18:1/22:1 814.632 PC 18:1/20:1; 18:2/20:0 812.506 PE 17:2/24:0; 18:2/23:0; 19:1/22:1 826.538 PC18:3/21:0 [M − H]− Phosphatidylglycerol 828.553 PC 18:2/21:0 719.485 PG 16:0/16:1; 14:0/18:1 836.616 PC 20:2/20:3 721.499 PG 16:0/16:0; 14:0/18:0 838.601 PC 18:3/22:1; 18:2/22:2 741.468 PG 16:0/18:3; 16:0/18:4 840.617 PC 18:3/22:0; 18:2/22:1 743.485 PG 16:0/18:3; 16:1/18:2 842.631 PC 18:2/22:0; 18:1/22:1 745.499 PG 16:0/18:2; 16:1/18:1 844.647 PC 18:1/22:0 747.516 PG 16:0/18:1 868.649 PC 18:3/24:0; 18:2/24:1 749.525 PG 16:0/18:0 870.664 PC 18:1/24:1; 18:2/24:0 765.475 PG 18:3/18:3; 16:1/20:5 872.680 PC 18:1/24:0 767.485 PG 18:2/18:3 874.725 PC 18:0/24:0 769.543 PG 18:1/18:3; 18:2/18:2 [M − H]− Phosphatidic acid 771.515 PG 18:0/18:3; 18:1/18:2 667.433 PA 16:1/18:3; 16:0/18:4; 16:2/18:2; 16:3/18:1 773.532 PG 18:1/18:1; 18:0/18:2 669.448 PA 16:0/18:3; 16:1/18:2 [M − H]− Phosphatidylinositol 671.464 PA 16:0/18:2 831.499 PI 16:0/18:3 673.479 PA 16:0/18.1 833.515 PI 16:0/18:2 675.486 PA 16:0/18:0 855.498 PI 18:2/18:3 857.513 PI 18:1/18:3; 18:2/18:2 861.545 PI 18:0/18:2; 18:1/18:1" pageId="3" pageNumber="97">Table 4</tableCitation>
|
|||
|
|
. The main PC molecular species were PC 16:0/18:2 and PC 16:1/18:1 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505C8E98BCFA8FADC" bold="true" box="[464,500,1275,1294]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
756.556), PC 16:0/18:1 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1506FFE98BCE31FAF8" bold="true" italics="true" pageId="3" pageNumber="97">m/ z</emphasis>
|
|||
|
|
760.585), PC 18:1/18:3 and PC 18:2/18:2 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150634E867CC0DFAF8" bold="true" box="[556,593,1303,1322]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
782.566) and PC (18:1/18:1), PC (18:0/18:2) and PC (16:2/20:0) (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15065BE843CC34FA94" bold="true" box="[579,616,1331,1350]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
786.601). Odd-chain FA's were found in some PC molecular species, such as C17:0, C19:0, C21:0, as well as molecular species with very long chain FA (PC 18:3/24:0; PC 18:1/24:1). The main PE molecular species in
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC1506B7E8F7CEF7FA64" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1506B7E8F7CEF7FA64" bold="true" italics="true" pageId="3" pageNumber="97">H. portulacoides</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
lipid extracts were identified at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505FEE8D3CC56FA64" bold="true" box="[486,522,1443,1462]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
714.505, identified as PE 16:0/18:2 and PE 16:1/18:1, and at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505A7E8CFCFBFFA00" bold="true" box="[447,483,1471,1490]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
738.505 assigned as PE 18:2/ 18:2 and PE 18:1/18:3. Some of the molecular species contained odd chain FAs such as PE 18:2/21:0 and PE 17:2/24:0. In the PA class, the molecular species identified were esterified to FA with 16 and 18 carbon atoms. The main molecular species in the PA class were PA 16:0/18:2 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1504C5EB3ACF5EF98F" bold="true" box="[221,258,1610,1629]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
671.464 and PA 18:2/18:2 and 18:1/18:3 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1506C5EB3ACD5EF98F" bold="true" box="[733,770,1610,1629]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
695.464. The acyl composition of PG ranged from 14 to 20 carbon atoms. PG 16:0/18:3 and PG 16:1/18:2 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505EBEBF2CC44F947" bold="true" box="[499,536,1666,1685]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
743.485) and PG 16:0/ 18:2 and 16:1/18:1 (
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150535EBEECF61F963" bold="true" box="[301,317,1694,1713]" italics="true" pageId="3" pageNumber="97">m</emphasis>
|
|||
|
|
/
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150550EBEECF0DF963" bold="true" box="[328,337,1694,1713]" italics="true" pageId="3" pageNumber="97">z</emphasis>
|
|||
|
|
745.499) were the most abundant molecular species. In this same class a molecular specie with C18:4 (PG 16:0/18:4) was detected at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1504E4EBA5CF50F93A" bold="true" box="[252,268,1749,1768]" italics="true" pageId="3" pageNumber="97">m</emphasis>
|
|||
|
|
/
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15050FEBA5CF7CF93A" bold="true" box="[279,288,1749,1768]" italics="true" pageId="3" pageNumber="97">z</emphasis>
|
|||
|
|
741.468. The molecular species of the PI class had fatty acyl groups with 16 and 18 carbon atoms and with 0–3 double bonds, as was observed for PA class. The most abundant molecular specie was found at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15053FEA59CF17F8EE" bold="true" box="[295,331,1833,1852]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
833.515, corresponding to PI 16:0/18:2.
|
|||
|
|
</paragraph>
|
|||
|
|
<paragraph id="8BB236E9FFB3EC15049DEA35CAC5FBD1" blockId="3.[100,771,884,1992]" lastBlockId="3.[818,1488,757,1139]" pageId="3" pageNumber="97">
|
|||
|
|
The analysis of the lipid extracts of
|
|||
|
|
<taxonomicName id="4C0D4D6AFFB3EC1505F8EA35CC2EF88A" box="[480,626,1861,1880]" class="Magnoliopsida" family="Chenopodiaceae" genus="Halimione" higherTaxonomySource="GBIF" kingdom="Plantae" order="Caryophyllales" pageId="3" pageNumber="97" phylum="Magnoliophyta" rank="species" species="portulacoides">
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1505F8EA35CC2EF88A" bold="true" box="[480,626,1861,1880]" italics="true" pageId="3" pageNumber="97">H. portulacoides</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
revealed three glycolipid classes: SQDG, MGDG and DGDG and one glycosphingolipid, HexCer. In total, 39 molecular species were identified: 18 SQDG, 10 MGDG, 8 DGDG and 3 HexCer (
|
|||
|
|
<tableCitation id="C68F0352FFB3EC150583EAE9CFBFF87E" box="[411,483,1945,1964]" captionStart="Table 5" captionStartId="4.[100,150,1546,1563]" captionTargetPageId="4" captionText="Table 5 Glycolipids molecular species identified by HILIC−Q−Exactive−MS from a total lipid extract of Halimione portulacoides. [M − H]− Sulfoquinovosyldiacylglycerol 932.626 DGDG 18:3/16:0 787.465 SQDG 18:3/14:0; 16:3/16:0 938.665 DGDG 18:0/16:0 789.48 SQDG 18:2/14:0 954.611 DGDG 18:3/18:3 791.497 SQDG 16:1/16:0 960.655 DGDG 18:3/18:0; 18:2/18:1;20:3/16:0 793.512 SQDG 16:0/16:0 [M + NH]+ Monogalactosyldiacylglycerol 4 801.48 SQDG 18:3/15:0 764.527 MGDG 18:3/16:3 813.481 SQDG 18:3/16:1 768.560 MGDG 18:1/16:3; 18:2/16:2; 18:3/16:1 815.497 SQDG 18:3/16:0 770.574 MGDG 18:3/16:0; 18:2/16:1 817.512 SQDG 18:1/16:1; 20:0/14:2 772.588 MGDG 18:2/16:0 837.480 SQDG 18:3/18:3 774.601 MGDG 18:1/16:0 839.496 SQDG 18:3/18:2 792.558 MGDG 18:3/18:3 843.5275 SQDG 18:2/18:1; 18:3/18:0; 20:3/16:0; 20:0/16:3 808.553 MGDG 19:2/18:3 845.5417 SQDG 18:2/18:0; 18:1/18:1 [M + H]+ Hexosylceramide [M + NH]+ Digalactosyldiacylglycerol 714.549 HexCer d18:1/h16:1 4 910.641 DGDG 16:0/16:0 816.653 HexCer t18:1/h22:0 926.580 DGDG 18:3/16:3 844.684 HexCer t18:1/h24:0" pageId="3" pageNumber="97">Table 5</tableCitation>
|
|||
|
|
). The acyl composition of GL molecular species ranged from 14 to 20 carbon atoms and 0 to 3 double bonds. The details of all GL molecular species that were identified are shown in
|
|||
|
|
<tableCitation id="C68F0352FFB3EC150797EE61CD8AFCF6" box="[911,982,785,804]" captionStart="Table 3" captionStartId="3.[100,150,161,178]" captionTargetPageId="3" captionText="Table 3 Glycolipid molecular species identified by HILIC−Q−Exactive−MS from a total lipid extract of Salicornia ramosissima. [M − H]− Sulfoquinovosyldiacylglycerol [M + NH]+ Monogalactosyldiacylglycerol 4 793.511 SQDG 16:0/16:0 784.513 MGDG 18:3/17:0; 19:3/16:0 815.496 SQDG18:3/16:0 786.525 MGDG 18:2/17:0; 19:2/16:0 819.529 SQDG 18:1/16:0 792.558 MGDG 18:3/18:3 831.49 SQDG 19:2/16:0 798.637 MGDG 18:3/18:0; 18:2/18:1 837.479 SQDG 18:3/18:3 806.537 MGDG 19:3/18:3 841.511 SQDG 18:3/18:1; 18:2/18:2 808.553 MGDG 19:3/18:2; 19:2/18:3 843.526 SQDG 18:3/18:0 824.651 MGDG 20:1/18:3 847.485 SQDG 20:1/16:0 830.683 MGDG 22:1/16:0; 17:1/21:0 853.474 SQDG 19:2/18:3 832.699 MGDG 22:0/16:0 863.48 SQDG 20:5/18:2; 22:6/16:1 840.644 MGDG 21:0/18:3; 20:0/19:3; 20:2/19:1 869.469 SQDG 18:3/20:1 848.629 MGDG 18:3/22:3 [M + NH]+ Digalactosyldiacylglycerol 852.669 MGDG 18:3/22:1 4 932.627 DGDG 18:3/16:0 854.684 MGDG 18:3/22:0; 18:2/22:1 936.653 DGDG 18:1/16:0 [M + H]+ Hexosylceramide 954.612 DGDG 18:3/18:3 714.549 HexCer d18:1/h16:1 958.644 DGDG 18:3/18:1; 18:2/18:2 816.653 HexCer t18:1/h22:0 960.656 DGDG 18:3/18:0; 18:2/18:1 830.684 HexCer t18:1/h23:0 970.607 DGDG 19:2/18:3 844.684 HexCer t18:1/h24:0 986.601 DGDG 20:1/18:3 846.69 HexCer t18:0/h24:0 1002.596 DGDG 21:0/18:3" pageId="3" pageNumber="97">Table 3</tableCitation>
|
|||
|
|
. The main SQDG molecular species was SQDG 18:3/ 16:0 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15076EEE5DCDC7FC92" bold="true" box="[886,923,813,832]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
815.497, followed by SQDG 16:0/16:0 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150134EE5DCB0CFC92" bold="true" box="[1324,1360,813,832]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
793.512 and SQDG 18:3/18:2 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1507F1EE39CA51FC8E" bold="true" box="[1001,1037,841,860]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
839.496. The most abundant molecular species in MGDG observed in LC− MS was the MGDG 18:3/18:3 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC1501B3EE15CB93FCAA" bold="true" box="[1451,1487,869,888]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
792.558. Only one MGDG molecular species with odd-chain FA was observed, the MGDG 119:2/18:3 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC15009AEEEDCAFBFC62" bold="true" box="[1154,1191,925,944]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
808.553. The fatty acyl chains of DGDG comprised 16 or 18 carbon atoms. The main molecular species was DGDG 18:3/18:3 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150001EEA5CA61FC3A" bold="true" box="[1049,1085,981,1000]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
954.611. No molecular species with odd-chain FA were identified in this class.
|
|||
|
|
</paragraph>
|
|||
|
|
<paragraph id="8BB236E9FFB3EC15074BE97CCAD5FBA1" blockId="3.[818,1488,757,1139]" pageId="3" pageNumber="97">
|
|||
|
|
The analysis of HexCer showed the presence of both di- and trihydroxy long-chain bases. The long chain bases were C18, while the fatty acids were hydroxylated. The most abundant molecular species was HexCer
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150766E910CDD8FBA1" bold="true" box="[894,900,1120,1139]" italics="true" pageId="3" pageNumber="97">t</emphasis>
|
|||
|
|
18:1/h24:0 at
|
|||
|
|
<emphasis id="B979EAFBFFB3EC150011E910CA72FBA1" bold="true" box="[1033,1070,1120,1139]" italics="true" pageId="3" pageNumber="97">m/z</emphasis>
|
|||
|
|
844.684.
|
|||
|
|
</paragraph>
|
|||
|
|
</subSubSection>
|
|||
|
|
</treatment>
|
|||
|
|
</document>
|