<documentid="619C7997C1A9BA638FBE07A373F49083"ID-DOI="10.11646/phytotaxa.388.1.2"ID-ISSN="1179-3163"ID-Zenodo-Dep="13725500"IM.bibliography_approvedBy="felipe"IM.illustrations_approvedBy="felipe"IM.materialsCitations_approvedBy="felipe"IM.metadata_approvedBy="felipe"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="felipe"checkinTime="1725673815287"checkinUser="felipe"docAuthor="Darienko, Tatyana & Pröschold, Thomas"docDate="2019"docId="03BBD82074369832FF2DFC4FFEB6F9A8"docLanguage="en"docName="phytotaxa.388.1.2.pdf"docOrigin="Phytotaxa 388 (1)"docSource="http://dx.doi.org/10.11646/phytotaxa.388.1.2"docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article"docStyleId="96748F8F1B6C902996E134952A3A36B9"docStyleName="Phytotaxa.2014-.journal_article"docStyleVersion="13"docTitle="Jaagichlorella roystonensis var. epilithica Darienko & Proschold 2019, var. nov."docType="treatment"docVersion="2"lastPageNumber="64"masterDocId="FF82A05874279823FFA5FFF9FFB7FFD5"masterDocTitle="The genus Jaagichlorella Reisigl (Trebouxiophyceae, Chlorophyta) and its close relatives: an evolutionary puzzle"masterLastPageNumber="68"masterPageNumber="47"pageNumber="64"updateTime="1725685413678"updateUser="ExternalLinkService"zenodo-license-figures="UNSPECIFIED"zenodo-license-treatments="UNSPECIFIED">
<mods:titleid="D4BE727D8A457D59FA9A49DDB10ABB64">The genus Jaagichlorella Reisigl (Trebouxiophyceae, Chlorophyta) and its close relatives: an evolutionary puzzle</mods:title>
<mods:affiliationid="D9D2690685754080AEE4B67E6CF68798">University of Göttingen, Albrecht-von-Haller-Institute of Plant Sciences, Experimental Phycology and Sammlung für Algenkulturen, D- 37073 Göttingen, Germany & M. G. Kholodny Institute of Botany, National Academy Science of Ukraine, Kyiv 01601, Ukraine</mods:affiliation>
<mods:affiliationid="650FF95D41064CEF36E1DAEF64D9C21F">University of Innsbruck, Research Department for Limnology, A- 5310 Mondsee, Austria</mods:affiliation>
<taxonomicNameid="4C1212B574369832FF2DFC4FFCD9FC04"authority="Darienko & Proschold"authorityName="Darienko & Proschold"authorityYear="2019"box="[136,878,950,977]"class="Trebouxiophyceae"family="Oocystaceae"genus="Jaagichlorella"kingdom="Plantae"order="Oocystales"pageId="17"pageNumber="64"phylum="Chlorophyta"rank="variety"species="roystonensis"status="var. nov."variety="epilithica">
<figureCitationid="132975B374369832FC47FC41FBEBFC04"box="[994,1116,952,977]"captionStart="FIGURE 5"captionStartId="11.[136,229,1427,1448]"captionTargetBox="[189,1398,190,1402]"captionTargetId="figure-14@11.[189,1398,190,1402]"captionTargetPageId="11"captionText="FIGURE 5. Morphology and phenotypic plasticity of Jaagichlorella roystonensis var. epilithica (SAG 2133; A.–H.) and J. roystonensis var. handai (SAG 2198; I.–G‘.); scale bar = 10 μm."figureDoi="http://doi.org/10.5281/zenodo.13725512"httpUri="https://zenodo.org/record/13725512/files/figure.png"pageId="17"pageNumber="64">Fig. 5A–H</figureCitation>
Young cells are spherical or sometimes irregular with relative thick cell wall, 6.4–7.3 μm in size. Chloroplast by young cells saucer-shaped, covers a little bit more than a half of the cell; with a single pyrenoid surrounded by many starch grains.
</paragraph>
<paragraphid="8BAD693674369832FF18FB95FD88FAC0"blockId="17.[136,1452,1024,1661]"pageId="17"pageNumber="64">Mature vegetative cells are spherical 9.1–10.0 μm, sometimes 11.8 μm in diameter, with cup-shaped chloroplast, sometimes slightly removed from the cell wall in the bottom part of the cell and occupied the middle position of the cell; chloroplast relatively thick, green or yellowish-green. Cells with numerous, colorless vacuoles. Old cells are spherical, 10.9–13.6 μm in size, sometimes up to 17,3 μm, with reduced small chloroplast, cells contain several vacuoles and fill around 1/3 of the cell.</paragraph>
Reproduction is by 2-4-8 unequal autospores. Autosporangia are spherical or very often irregular, (8.2)–9.1–10.0 μm, sometimes they can reach 16.4–20.9 μm. Large autospores 6.4–7.3 μm, (up to 10.0 μm), small autospores 3.7–5.4 μm in size. SSU and ITS rDNA sequences (GenBank: MH780940) and ITS-2 Barcode BC-3a in
<figureCitationid="132975B374369832FB63FA91FABDFA54"box="[1222,1290,1384,1409]"captionStart="FIGURE 3"captionStartId="9.[136,229,1113,1134]"captionTargetBox="[171,1421,208,1067]"captionTargetId="figure-14@9.[151,1436,187,1085]"captionTargetPageId="9"captionText="FIGURE 3. Comparison of the conserved region of ITS-2 among the species of Jaagichlorella and Kalinella. Extraction of this region and translation into a number code for its usage as barcode. The ‘/’ indicated the position of the site loop in Helix III, which we excluded from the barcode. Number code for each base pair: 1 = A-U; 2 = U-A; 3 = G-C; 4 = C-G; 5 = G•U; 6 = U•G; 7 = pyrimidine-pyrimidine mismatch; 8 = deletion, single or unpaired bases. The phylogenetic tree shown was calculated using the neighbor-joining method based on the data set (69 number-coded positions of 11 haplotypes) using PAUP 4.0b10."figureDoi="http://doi.org/10.5281/zenodo.13725508"httpUri="https://zenodo.org/record/13725508/files/figure.png"pageId="17"pageNumber="64">Fig. 3</figureCitation>
<materialsCitationid="3B7A636B74369832FF18FA49FC11FA38"collectionCode="SAG"country="Germany"location="Culture Collection of Algae"municipality="University of Gottingen"pageId="17"pageNumber="64"specimenCode="SAG 2133"specimenCount="1"typeStatus="holotype">
<specimenCodeid="DBB4C14D74369832FD4EFA49FCD5FA1C"box="[747,866,1456,1481]"collectionCode="SAG"country="Germany"httpUri="http://grbio.org/cool/2vvw-hn58"name="Sammlung von Algenkulturen at Universitat Gottingen"pageId="17"pageNumber="64">SAG 2133</specimenCode>
is cryopreserved in a metabolic inactive state at the
<locationid="8ECD3FED74369832FF2DFA2DFE08FA38"LSID="urn:lsid:plazi:treatment:03BBD82074369832FF2DFC4FFEB6F9A8:8ECD3FED74369832FF2DFA2DFE08FA38"box="[136,447,1492,1517]"country="Germany"municipality="University of Gottingen"name="Culture Collection of Algae"pageId="17"pageNumber="64">Culture Collection of Algae</location>
(
<collectionCodeid="ED03F1F374369832FE68FA2DFDBDFA38"box="[461,522,1492,1517]"country="Germany"httpUri="http://grbio.org/cool/2vvw-hn58"name="Sammlung von Algenkulturen at Universitat Gottingen"pageId="17"pageNumber="64">SAG</collectionCode>
),
<collectingMunicipalityid="6BC9F34C74369832FDBBFA2DFC9AFA38"box="[542,813,1492,1517]"pageId="17"pageNumber="64">University of Göttingen</collectingMunicipality>
<figureCitationid="132975B374369832FCB0FA01FCDEF9C4"box="[789,873,1528,1553]"captionStart="FIGURE 5"captionStartId="11.[136,229,1427,1448]"captionTargetBox="[189,1398,190,1402]"captionTargetId="figure-14@11.[189,1398,190,1402]"captionTargetPageId="11"captionText="FIGURE 5. Morphology and phenotypic plasticity of Jaagichlorella roystonensis var. epilithica (SAG 2133; A.–H.) and J. roystonensis var. handai (SAG 2198; I.–G‘.); scale bar = 10 μm."figureDoi="http://doi.org/10.5281/zenodo.13725512"httpUri="https://zenodo.org/record/13725512/files/figure.png"pageId="17"pageNumber="64">Fig. 5C</figureCitation>
