<documentid="CC21C1B2717B145C38BE668B9C28BFDA"ID-DOI="10.5852/ejt.2023.870.2115"ID-GBIF-Dataset="b9eee532-7f47-4602-aee6-83572065d777"ID-ISSN="2118-9773"ID-Zenodo-Dep="7938573"IM.bibliography_approvedBy="julia"IM.illustrations_approvedBy="julia"IM.materialsCitations_approvedBy="felipe"IM.metadata_approvedBy="valdenar"IM.tables_approvedBy="julia"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="valdenar"checkinTime="1684177557466"checkinUser="felipe"docAuthor="Raghoonundon, Bhavesh, Vadthanarat, Santhiti, Hu, Yuwei & Raspé, Olivier"docDate="2023"docId="737D8341B614FFD1FE30F990FBAFFC9F"docLanguage="en"docName="ejt-870_30-45.pdf"docOrigin="European Journal of Taxonomy 870"docSource="http://dx.doi.org/10.5852/ejt.2023.870.2115"docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1"docStyleId="EF2B578F1D15862ADE45B0C07C620911"docStyleName="EJT.2018-.journal_article.type1"docStyleVersion="14"docTitle="Inocybe hopeae Raghoonundon & Raspe 2023, sp. nov."docType="treatment"docVersion="6"lastPageNumber="39"masterDocId="8F44FB39B610FFD8FFC6FFA4FF9AFFCD"masterDocTitle="Inocybe hopeae sp. nov. and first record of Pseudosperma keralense (Inocybaceae) from Thailand"masterLastPageNumber="45"masterPageNumber="30"pageNumber="34"updateTime="1732566758404"updateUser="ExternalLinkService"zenodo-license-document="CC-BY-4.0"zenodo-license-figures="CC-BY-4.0"zenodo-license-treatments="UNSPECIFIED">
<mods:titleid="7FB06BDF0DE951F6D14D342C1973E396">Inocybe hopeae sp. nov. and first record of Pseudosperma keralense (Inocybaceae) from Thailand</mods:title>
<mods:affiliationid="4979270CF14D5CD84590874E1EF072F3">School of Science, Mae Fah Luang University, Chiang Rai, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand.</mods:affiliation>
<mods:affiliationid="86FDDE50C702F85B417E2777297AC22D">School of Science, Mae Fah Luang University, Chiang Rai, Thailand. & Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand. & School of Science, Mae Fah Luang University, Chiang Rai, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand. & Centre for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.</mods:affiliation>
<mods:affiliationid="E2FB108CFD28666202F6E65F428587CF">School of Science, Mae Fah Luang University, Chiang Rai, Thailand. & Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand. & School of Science, Mae Fah Luang University, Chiang Rai, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand. & Centre for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.</mods:affiliation>
<taxonomicNameid="3CD449D4B614FFDCFE30F990FC46F983"ID-CoL="CB8JC"authority="Raghoonundon & Raspe"authorityName="Raghoonundon & Raspe"authorityYear="2023"box="[502,988,1588,1615]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="4"pageNumber="34"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
<figureCitationid="63EF2ED2B614FFDCFD21F9D9FCD6F95A"box="[743,844,1661,1687]"captionStart-0="Fig"captionStart-1="Fig"captionStart-2="Fig"captionStartId-0="7.[189,233,1928,1954]"captionStartId-1="8.[189,233,1929,1955]"captionStartId-2="9.[189,234,1847,1873]"captionTargetBox-0="[190,1397,466,1888]"captionTargetBox-1="[230,1320,285,1843]"captionTargetBox-2="[189,1398,911,1809]"captionTargetId-0="figure-57@7.[189,1398,465,1890]"captionTargetId-1="figure-11@8.[218,1369,265,1892]"captionTargetId-2="figure-277@9.[189,1398,911,1809]"captionTargetPageId-0="7"captionTargetPageId-1="8"captionTargetPageId-2="9"captionText-0="Fig. 2. a. Basidiomes of Inocybe hopeae Raghoonundon & Raspé sp. nov. (OR1665, holotype). c–d. Basidiomes of Inocybe hopeae (OR1630). b, e–f. Basidiomes of Pseudosperma keralense (K.P.D.Latha & Manim.) Matheny & Esteve-Rav. (OR1629). Scale bars: a = 10 mm, b–d = 5 mm."captionText-1="Fig. 3. Microscopic features of Inocybe hopeae Raghoonundon & Raspé sp. nov. a. Caulocystidia. b. Pleurocystidia. c. Cheilocystidia. d. Basidia. e. Pileipellis. f. Basidiospores. Scale bars: a–e = 20 µm, f = 10 µm."captionText-2="Fig. 4. Scanning electron micrograph of basidiospores from the holotype of Inocybe hopeae Raghoonundon & Raspé sp. nov. (OR1665). Scale bar = 10 µm."figureDoi-0="http://doi.org/10.5281/zenodo.7938577"figureDoi-1="http://doi.org/10.5281/zenodo.7938583"figureDoi-2="http://doi.org/10.5281/zenodo.7938587"httpUri-0="https://zenodo.org/record/7938577/files/figure.png"httpUri-1="https://zenodo.org/record/7938583/files/figure.png"httpUri-2="https://zenodo.org/record/7938587/files/figure.png"pageId="4"pageNumber="34">Figs 2–4</figureCitation>
<taxonomicNameid="3CD449D4B614FFDCFF7BF94BFEE2F8C4"authority="Raghoonundon & Vadthanarat & Raspé, 2023"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[189,376,1775,1801]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="4"pageNumber="34"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
<taxonomicNameid="3CD449D4B614FFDCFD28F94BFC46F8C4"authority="E.Horak, Matheny & Desjardin"box="[750,988,1775,1801]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="4"pageNumber="34"phylum="Basidiomycota"rank="species"species="thailandica">
by having wider brownish orange to brown pileus darker towards the margin, thicker off-white to pale brown context, light brown to dark brown stipe, pleurocystidia being different from cheilocystidia, broader and longer basidia and caulocystidia.
<taxonomicNameid="3CD449D4B614FFDCFE95F86AFE32F825"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[339,424,1998,2024]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="4"pageNumber="34"phylum="Basidiomycota"rank="species"species="hopeae">
<taxonomicNameid="3CD449D4B615FFDDFB0FFEADFCE6FE8A"authority="(K. P. D. Latha & Manim.) Matheny & Esteve-Rav."authorityName="Matheny & Esteve-Rav."baseAuthorityName="K. P. D. Latha & Manim."class="Agaricomycetes"family="Inocybaceae"genus="Pseudosperma"kingdom="Fungi"order="Agaricales"pageId="5"pageNumber="35"phylum="Basidiomycota"rank="species"species="keralense">
<taxonomicNameid="3CD449D4B615FFDDFE55F8C2FCF5F84D"authority="Matheny & Esteve-Rav."authorityName="Matheny & Esteve-Rav."box="[403,879,1894,1920]"class="Agaricomycetes"family="Inocybaceae"genus="Pseudosperma"kingdom="Fungi"order="Agaricales"pageId="5"pageNumber="35"phylum="Basidiomycota"rank="genus">
<taxonomicNameid="3CD449D4B615FFDDFBA2F8C2FED4F86E"authority="Matheny & K. P. D. Latha"authorityName="Matheny & K. P. D. Latha"authorityYear="2019"class="Agaricomycetes"family="Inocybaceae"genus="Nothocybe"kingdom="Fungi"order="Agaricales"pageId="5"pageNumber="35"phylum="Basidiomycota"rank="genus">
were used as outgroup taxa. Maximum likelihood bootstrap (MLB, left) ≥70% and Bayesian posterior probabilities (BPP, right) ≥ 0.95 are shown above supported branches. The new species and new record are in bold.
<collectingRegionid="3910FCB5B616FFDEFEBDFEC5FD19FEB6"box="[379,643,353,379]"country="Thailand"name="Chiang Rai"pageId="6"pageNumber="36">Chiang Rai Province</collectingRegion>
<collectingRegionid="3910FCB5B616FFDEFEA2FDBDFDD5FDFE"box="[356,591,537,563]"country="Thailand"name="Chiang Rai"pageId="6"pageNumber="36">Chiang Rai province</collectingRegion>
<collectingRegionid="3910FCB5B616FFDEFB9DFD9BFAD5FD94"box="[1115,1359,575,601]"country="Thailand"name="Chiang Rai"pageId="6"pageNumber="36">Chiang Rai Province</collectingRegion>
in diameter, at first obtusely conical, becoming umbonate to plano-umbonate when mature, margin deflexed; surface dull and dry, fibrillose to striate with recurved scales, brownish orange (5C4–5C7) to brown (6D5–6E6), dark brown (6F6–7F6) near the margin; thin context
, slightly bulbous; surface even, dull and dry, with minute squamules at the top, elsewhere glabrous, light brown (6D4–7D5) to dark brown (7F5), darker towards the top, unchanging when bruised; basal mycelium off-white (6A1), turning orange when bruised (6A7–6A8); context solid or fibrillose, fleshy, pale brown (6C4), staining slightly orange (5A3–5A4) when bruised. Hymenophore lamellate, adnexed to adnate with a decurrent tooth; lamellae 35–54 reaching stipe, 5–7 lamellulae, close, thick, ventricose, forked mostly near the margin, yellowish gray (4B1–4B2), becoming brown (5D5–6E5) with age, lamellae edge even. Smell and taste not distinctive.
Basidiospores [125/6/4] (5.3–)5.8–8.2–11.1(–14) µm in diam., globose to ovoid, with conical-spinose projections up to 3.9 µm long, sub–globose to globose, brownish in KOH and NH 4 OH. Basidia (33.2–)33.9–44–51.9(–52.3) × (9.8–)10.1–14.6–17.6(–17.7) µm, 4-spored, clavate, hyaline, sterigmata up to 8 µm long. Cheilocystidia (39–)39.5–47.3–71(–71) × (10.2–)10.2–14.9–20.2(–20.2) µm, frequent, thick-walled, fusiform to broadly fusiform with some scattered crystals at the top, hyaline, yellowish in KOH and NH
<taxonomicNameid="3CD449D4B616FFDEFF29F8FCFCA8F8BF"authority="Rehder & E. H. Wilson"authorityName="Rehder & E. H. Wilson"box="[239,818,1880,1906]"class="Magnoliopsida"family="Fagaceae"genus="Castanopsis"kingdom="Plantae"order="Fagales"pageId="6"pageNumber="36"phylum="Tracheophyta"rank="species"species="calathiformis">
<taxonomicNameid="3CD449D4B617FFDFFF7BFE93FEEEFE9C"authority="Raghoonundon & Vadthanarat & Raspé, 2023"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[189,372,311,337]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="7"pageNumber="37"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
is macro-morphologically characterized by medium sized basidiomes, brownish orange to brown pileus becoming darker towards the margin, off-white to pale brown context, light brown to dark brown stipe with off-white basal mycelium and pale brown to grayish brown lamellae. Molecular data (
<figureCitationid="63EF2ED2B619FFD1FEC4FEADFED0FEEE"box="[258,330,265,291]"captionStart="Fig"captionStartId="5.[189,232,1858,1884]"captionTargetBox="[189,1353,565,1806]"captionTargetId="figure-68@5.[189,1398,548,1820]"captionTargetPageId="5"captionText="Fig. 1. Maximum likelihood phylogenetic tree inferred from the three gene dataset (tef1, LSU, rpb2). Seven species of Pseudosperma Matheny & Esteve-Rav. and one species of Nothocybe Matheny & K.P.D.Latha were used as outgroup taxa. Maximum likelihood bootstrap (MLB, left) ≥70% and Bayesian posterior probabilities (BPP, right) ≥ 0.95 are shown above supported branches. The new species and new record are in bold."figureDoi="http://doi.org/10.5281/zenodo.7938575"httpUri="https://zenodo.org/record/7938575/files/figure.png"pageId="9"pageNumber="39">Fig. 1</figureCitation>
) confirmed a close relationship between
<taxonomicNameid="3CD449D4B619FFD1FC8FFEAEFC26FEEE"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[841,956,265,291]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
<taxonomicNameid="3CD449D4B619FFD1FF79FE89FEFBFE8B"authority="E.Horak, Matheny & Desjardin"box="[191,353,300,326]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">I. thailandica</taxonomicName>
.
</emphasis>
However, when compared to the latter, our species is morphologically different.
<taxonomicNameid="3CD449D4B619FFD1FADCFE88FED9FEA4"authority="E.Horak, Matheny & Desjardin"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
has a dark brown pileus becoming pale brown towards the margin, which is the opposite of
<taxonomicNameid="3CD449D4B619FFD1FF7BFED7FEB0FE41"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[189,298,370,396]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
, in which the brownish orange pileus is darker towards the margin. Moreover,
<taxonomicNameid="3CD449D4B619FFD1FB01FED7FAAEFE41"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[1223,1332,370,396]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
has a wider pileus and thicker context as compared to
<taxonomicNameid="3CD449D4B619FFD1FD3EFE33FC0CFE7D"authority="E.Horak, Matheny & Desjardin"box="[760,918,406,432]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
<taxonomicNameid="3CD449D4B619FFD1FBC3FE33FB3EFE7D"authority="E.Horak, Matheny & Desjardin"box="[1029,1188,406,432]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
has a light brown stipe that is darker towards the base, whereas in
<taxonomicNameid="3CD449D4B619FFD1FD32FE1EFCFBFE1E"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[756,865,441,467]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
<taxonomicNameid="3CD449D4B617FFDFFE38F82CFC65F86F"authority="Raghoonundon & Raspe"authorityName="Raghoonundon & Raspe"authorityYear="2023"box="[510,1023,1928,1954]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="7"pageNumber="37"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
<taxonomicNameid="3CD449D4B617FFDFFB87F80FFC9FF824"authority="(K. P. D. Latha & Manim.) Matheny & Esteve-Rav."authorityName="Matheny & Esteve-Rav."baseAuthorityName="K. P. D. Latha & Manim."class="Agaricomycetes"family="Inocybaceae"genus="Pseudosperma"kingdom="Fungi"order="Agaricales"pageId="7"pageNumber="37"phylum="Basidiomycota"rank="species"species="keralense">
<taxonomicNameid="3CD449D4B618FFD0FD80F82DFBADF869"authority="Raghoonundon & Raspe"authorityName="Raghoonundon & Raspe"authorityYear="2023"box="[582,1079,1929,1956]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="8"pageNumber="38"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
<taxonomicNameid="3CD449D4B619FFD1FD5FFE5FFC92FDD9"authority="Raghoonundon & Vadthanarat & Raspé, 2023"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[665,776,506,532]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae"status="sp. nov.">
<taxonomicNameid="3CD449D4B619FFD1FB17FE5FFAF5FDD9"authority="E.Horak, Matheny & Desjardin"box="[1233,1391,506,532]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
<taxonomicNameid="3CD449D4B619FFD1FD9CFDBAFD5FFDF5"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[602,709,542,568]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
have broader and longer basidia (33.2–52.3 × 9.8–17.7 µm) compared to
<taxonomicNameid="3CD449D4B619FFD1FE92FDE6FE68FD96"authority="E.Horak, Matheny & Desjardin"box="[340,498,577,603]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
<taxonomicNameid="3CD449D4B619FFD1FC08FDE6FBA1FD96"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[974,1083,577,603]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
(32–58.1 × 13.7–23.2 µm) also are longer and broader than those of
<taxonomicNameid="3CD449D4B619FFD1FD4FFDC1FCB8FDB3"authority="E.Horak, Matheny & Desjardin"box="[649,802,612,638]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
(20–36 × 6–12 µm). Furthermore, the cheilocystidia of
<taxonomicNameid="3CD449D4B619FFD1FF1AFD2CFEE3FD6C"authority="E.Horak, Matheny & Desjardin"box="[220,377,647,673]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
(2015) mentioned the pleurocystidia and cheilocystidia of
<taxonomicNameid="3CD449D4B619FFD1FB6AFD0FFAD6FD09"authority="E.Horak, Matheny & Desjardin"box="[1196,1356,682,708]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
<taxonomicNameid="3CD449D4B619FFD1FDF2FD6AFD3BFD25"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[564,673,718,744]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">
, whereby the pleurocystidia are narrower and shorter than the cheilocystidia. The basidiomes of
<taxonomicNameid="3CD449D4B619FFD1FDF9FD56FD43FCC6"authority="E.Horak, Matheny & Desjardin"box="[575,729,753,779]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="thailandica">
<taxonomicNameid="3CD449D4B619FFD1FD97FCB1FD26FCE3"authorityName="Raghoonundon & Vadthanarat & Raspé"authorityYear="2023"box="[593,700,788,814]"class="Agaricomycetes"family="Inocybaceae"genus="Inocybe"kingdom="Fungi"order="Agaricales"pageId="9"pageNumber="39"phylum="Basidiomycota"rank="species"species="hopeae">