243 lines
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243 lines
27 KiB
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<document id="F720C218075C5B6AE2F4E6D5021F1E5C" ID-DOI="10.1016/j.ijppaw.2017.12.001" ID-ISSN="2213-2244" ID-PMC="PMC5730365" ID-PubMed="29270360" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1708029473736" checkinUser="felipe" docAuthor="Gjerde, Bjørn, Vikøren, Turid & Hamnes, Inger Sofie" docDate="2018" docId="03CF87A3FF9DFFD0747CFA13FA8674B7" docLanguage="en" docName="IntJParasitolParasitesWildlife.7.1.1-11.pdf" docOrigin="International Journal for Parasitology: Parasites and Wildlife 7 (1)" docSource="http://dx.doi.org/10.1016/j.ijppaw.2017.12.001" docStyle="DocumentStyle:77CC2CF2FBA7BCD129DD16D0D4F96931.1:IntJParasitolParasitesWildlife.2012-.journal_article" docStyleId="77CC2CF2FBA7BCD129DD16D0D4F96931" docStyleName="IntJParasitolParasitesWildlife.2012-.journal_article" docStyleVersion="1" docTitle="Sarcocystis halieti Gjerde & Vikøren & Hamnes 2018" docType="treatment" docVersion="2" lastPageNumber="6" masterDocId="FFF6FFDBFF98FFD57418FFB0FFA3720F" masterDocTitle="Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway" masterLastPageNumber="11" masterPageNumber="1" pageNumber="6" updateTime="1708029787049" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-NC-ND-4.0">
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<mods:title id="B265867FE5B46CFB1DE1E84A4B29D959">Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway</mods:title>
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</mods:role>
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<mods:namePart id="539DE4D7E253F4540DDCA23DBE882FCA">Gjerde, Bjørn</mods:namePart>
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<mods:affiliation id="4A58E641E1A118FB69E11CE4BEAF4835">Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P. O. Box 8146 Dep., NO- 0033 Oslo, Norway</mods:affiliation>
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</mods:name>
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</mods:role>
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<mods:namePart id="E5717B91B5D74C8E50672C5EFE15BD3B">Vikøren, Turid</mods:namePart>
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<mods:affiliation id="0F59D28BF9371E5D6ACBE630340E4EDD">Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO- 0106 Oslo, Norway</mods:affiliation>
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</mods:name>
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</mods:role>
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<mods:namePart id="59BAE890C8AB8CE57FD9FF7542E440AD">Hamnes, Inger Sofie</mods:namePart>
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<mods:affiliation id="35873B39FF7E51A88DE64EED88BA2367">Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO- 0106 Oslo, Norway</mods:affiliation>
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<mods:title id="5E3B9A23B69187A6ECFF38DA575363A1">International Journal for Parasitology: Parasites and Wildlife</mods:title>
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<mods:part id="70DCCB923FD748218CE0E3426FDEE76D">
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<mods:date id="AC4A19B61806CCD367AB6A0CCD88D089">2018</mods:date>
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<mods:number id="FB4F8FCA597EC064E21939C3EDE181E8">2018-04-30</mods:number>
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<mods:number id="715EAEE1313D437A6C0FC1FDED29E898">1</mods:number>
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<mods:url id="B496645518CA6E1A434FC25246E3D45B">http://dx.doi.org/10.1016/j.ijppaw.2017.12.001</mods:url>
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<mods:classification id="D3EBC63B81DDD033D05DCB4108A81721">journal article</mods:classification>
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<mods:identifier id="B815F45DCADA08C589F997E6B80B12B5" type="DOI">10.1016/j.ijppaw.2017.12.001</mods:identifier>
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<mods:identifier id="5E82AE2DD55117F4ED216A5D80B46D61" type="ISSN">2213-2244</mods:identifier>
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<mods:identifier id="3ECC7089C4C85CE737E6A00A5A303103" type="PMC">PMC5730365</mods:identifier>
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<mods:identifier id="C634B92624026EF47819C7DAAD4A18B5" type="PubMed">29270360</mods:identifier>
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<treatment id="03CF87A3FF9DFFD0747CFA13FA8674B7" ID-DOI="http://doi.org/10.5281/zenodo.10668372" ID-Zenodo-Dep="10668372" LSID="urn:lsid:plazi:treatment:03CF87A3FF9DFFD0747CFA13FA8674B7" httpUri="http://treatment.plazi.org/id/03CF87A3FF9DFFD0747CFA13FA8674B7" lastPageNumber="6" pageId="5" pageNumber="6">
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<subSubSection id="C37C653EFF9DFFD0747CFA13FE6F77B9" box="[100,460,1443,1462]" pageId="5" pageNumber="6" type="nomenclature">
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<paragraph id="8BD936B5FF9DFFD0747CFA13FE6F77B9" blockId="5.[100,770,1443,1992]" box="[100,460,1443,1462]" pageId="5" pageNumber="6">
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<heading id="D09181D9FF9DFFD0747CFA13FE6F77B9" box="[100,460,1443,1462]" fontSize="36" level="2" pageId="5" pageNumber="6" reason="8">
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<emphasis id="B912EAA7FF9DFFD0747CFA13FE6F77B9" bold="true" box="[100,460,1443,1462]" italics="true" pageId="5" pageNumber="6">
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3.3.2. Ribosomal DNA unit of
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<taxonomicName id="4C664D36FF9DFFD07565FA13FE6F77B9" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[381,460,1443,1462]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">S. halieti</taxonomicName>
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C37C653EFF9DFFD0749DFA0EFA8674B7" pageId="5" pageNumber="6" type="description">
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<paragraph id="8BD936B5FF9DFFD0749DFA0EFB7B70E8" blockId="5.[100,770,1443,1992]" lastBlockId="5.[818,1488,585,1720]" pageId="5" pageNumber="6">
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As stated above, one species from the sea eagle, which we have named
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<taxonomicName id="4C664D36FF9DFFD074A8FA6AFEA777E2" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[176,260,1498,1517]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
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<emphasis id="B912EAA7FF9DFFD074A8FA6AFEA777E2" bold="true" box="[176,260,1498,1517]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
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</taxonomicName>
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, showed a high identity with GenBank sequences JQ733511–JQ733513 of an unnamed species from the great cormorant in
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<collectingCountry id="F3717625FF9DFFD0749AF9A2FF78742A" box="[130,219,1554,1573]" name="Lithuania" pageId="5" pageNumber="6">Lithuania</collectingCountry>
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(unpublished study by Prakas and co-workers), and is considered to be identical with that species. As regards the complete 18S rRNA gene, which was 1803 bp long, the new sequence (GenBank no.
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<collectionCode id="ED77AE70FF9DFFD07490F9D6FF097476" box="[136,170,1638,1657]" pageId="5" pageNumber="6">MF</collectionCode>
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946587) was 100% identical with GenBank sequence JQ733511 from the great cormorant, but it was also completely identical with sequences of
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<taxonomicName id="4C664D36FF9DFFD074C7F92EFE3174BE" box="[223,402,1694,1713]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="wobeseri">
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<emphasis id="B912EAA7FF9DFFD074C7F92EFE3174BE" bold="true" box="[223,402,1694,1713]" italics="true" pageId="5" pageNumber="6">Sarcocystis wobeseri</emphasis>
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</taxonomicName>
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(GenBank nos.
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<collectionCode id="ED77AE70FF9DFFD0763CF92EFDE574BE" box="[548,582,1694,1713]" pageId="5" pageNumber="6">GQ</collectionCode>
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922885, GQ922886,
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<collectionCode id="ED77AE70FF9DFFD0747CF909FF2974C3" box="[100,138,1721,1740]" country="0" name="Hastings Museum" pageId="5" pageNumber="6">HM</collectionCode>
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159419, EU502869). Moreover, the new sequence of
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<taxonomicName id="4C664D36FF9DFFD07692F909FD7974C3" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[650,730,1721,1740]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
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<emphasis id="B912EAA7FF9DFFD07692F909FD7974C3" bold="true" box="[650,730,1721,1740]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
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</taxonomicName>
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differed at only one nucleotide position (A rather than G in position 9 from the 3′ end) from GenBank sequence GQ245670 of
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<taxonomicName id="4C664D36FF9DFFD07653F941FD08750B" box="[587,683,1777,1796]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="calchasi">
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<emphasis id="B912EAA7FF9DFFD07653F941FD08750B" bold="true" box="[587,683,1777,1796]" italics="true" pageId="5" pageNumber="6">S. calchasi</emphasis>
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</taxonomicName>
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, but this difference seems to be due to the inclusion of the terminal reverse primer (Primer B) in that sequence (
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<bibRefCitation id="EFF74B44FF9DFFD075D0F899FDD87533" author="Olias, P. & Olias, L. & Lierz, M. & Mehlhorn, H. & Gruber, A. D." box="[456,635,1833,1852]" pageId="5" pageNumber="6" pagination="7 - 14" refId="ref14072" refString="Olias, P., Olias, L., Lierz, M., Mehlhorn, H., Gruber, A. D., 2010 b. Sarcocystis calchasi is distinct to Sarcocystis columbae sp. nov. from the wood pigeon (Columba palumbus) and Sarcocystis sp. from the sparrowhawk (Accipiter nisus). Vet. Parasitol. 171, 7 - 14. https: // doi. org / 10.1016 / j. vetpar. 2010.03.021." type="journal article" year="2010">Olias et al., 2010b</bibRefCitation>
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). This primer does not match sequences of various
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<taxonomicName id="4C664D36FF9DFFD075D7F8F5FDC37557" box="[463,608,1861,1880]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="undetermined">
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<emphasis id="B912EAA7FF9DFFD075D7F8F5FD927557" bold="true" box="[463,561,1861,1880]" italics="true" pageId="5" pageNumber="6">Sarcocystis</emphasis>
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spp.
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</taxonomicName>
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in that position, and it was therefore modified to Primer BSarc (
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<bibRefCitation id="EFF74B44FF9DFFD07607F8D1FD06757B" author="Gjerde, B." box="[543,677,1889,1908]" pageId="5" pageNumber="6" pagination="1591 - 1604" refId="ref12633" refString="Gjerde, B., 2014 b. Morphological and molecular characteristics of four Sarcocystis spp. in Canadian moose (Alces alces), including Sarcocystis taeniata n. sp. Parasitol. Res. 113, 1591 - 1604. https: // doi. org / 10.1007 / s 00436 - 014 - 3806 - z." type="journal article" year="2014">Gjerde, 2014b</bibRefCitation>
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). Thus,
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<taxonomicName id="4C664D36FF9DFFD076E9F8D1FF08759F" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="calchasi">
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<emphasis id="B912EAA7FF9DFFD076E9F8D1FF08759F" bold="true" italics="true" pageId="5" pageNumber="6">S. calchasi</emphasis>
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</taxonomicName>
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actually has a G in this position as can be ascertained from GenBank sequence KC733718. Hence, at least two species,
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<taxonomicName id="4C664D36FF9DFFD07685F829FCA175A3" box="[669,770,1945,1964]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="wobeseri">
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<emphasis id="B912EAA7FF9DFFD07685F829FCA175A3" bold="true" box="[669,770,1945,1964]" italics="true" pageId="5" pageNumber="6">S. wobeseri</emphasis>
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</taxonomicName>
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and
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<taxonomicName id="4C664D36FF9DFFD07496F805FF4F75C7" box="[142,236,1973,1992]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="calchasi">
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<emphasis id="B912EAA7FF9DFFD07496F805FF4F75C7" bold="true" box="[142,236,1973,1992]" italics="true" pageId="5" pageNumber="6">S. calchasi</emphasis>
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</taxonomicName>
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, were identical with
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<taxonomicName id="4C664D36FF9DFFD075AEF805FDA675C7" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[438,517,1973,1992]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
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<emphasis id="B912EAA7FF9DFFD075AEF805FDA675C7" bold="true" box="[438,517,1973,1992]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
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</taxonomicName>
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at the complete 18S rRNA gene. Moreover, sequences of this gene from several other
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<taxonomicName id="4C664D36FF9DFFD07175FDF9FCFA7077" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="undetermined">
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<emphasis id="B912EAA7FF9DFFD07175FDF9FA6C7053" bold="true" box="[1389,1487,585,604]" italics="true" pageId="5" pageNumber="6">Sarcocystis</emphasis>
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spp.
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</taxonomicName>
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with birds or carnivores as intermediate hosts (
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<taxonomicName id="4C664D36FF9DFFD07130FDD5FCC2709B" authority=", GenBank" authorityName="GenBank" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="columbae">
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<emphasis id="B912EAA7FF9DFFD07130FDD5FA367077" bold="true" box="[1320,1429,613,632]" italics="true" pageId="5" pageNumber="6">S. columbae</emphasis>
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, GenBank
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</taxonomicName>
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no.
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<collectionCode id="ED77AE70FF9DFFD0778AFD31FC1B709B" box="[914,952,641,660]" country="0" name="Hastings Museum" pageId="5" pageNumber="6">HM</collectionCode>
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125054;
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<taxonomicName id="4C664D36FF9DFFD07015FD31FAB8709B" authority=", GenBank" authorityName="GenBank" box="[1037,1307,641,660]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="corvusi">
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<emphasis id="B912EAA7FF9DFFD07015FD31FB15709B" bold="true" box="[1037,1206,641,660]" italics="true" pageId="5" pageNumber="6">Sarcocystis corvusi</emphasis>
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, GenBank
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</taxonomicName>
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no. JN256117;
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<taxonomicName id="4C664D36FF9DFFD071A6FD31FC7270BF" authority=", GenBank" authorityName="GenBank" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="turdusi">
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|
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<emphasis id="B912EAA7FF9DFFD071A6FD31FCCC70BF" bold="true" italics="true" pageId="5" pageNumber="6">S. turdusi</emphasis>
|
|||
|
|
, GenBank
|
|||
|
|
</taxonomicName>
|
|||
|
|
no. JF975681;
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07073FD2DFAB970BF" authority=", GenBank" authorityName="GenBank" box="[1131,1306,669,688]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="lutrae">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07073FD2DFB1470BF" bold="true" box="[1131,1207,669,688]" italics="true" pageId="5" pageNumber="6">S. lutrae</emphasis>
|
|||
|
|
, GenBank
|
|||
|
|
</taxonomicName>
|
|||
|
|
no.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD07150FD2DFACE70BF" box="[1352,1389,669,688]" country="Russia" name="Kotel'nich Museum" pageId="5" pageNumber="6">KM</collectionCode>
|
|||
|
|
657769;
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD071A6FD2DFC1870C3" authority=", GenBank" authorityName="GenBank" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="lari">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD071A6FD2DFCF370C3" bold="true" italics="true" pageId="5" pageNumber="6">S. lari</emphasis>
|
|||
|
|
, GenBank
|
|||
|
|
</taxonomicName>
|
|||
|
|
no. JQ733508) differed by only 1–6 nucleotides (99.7–99.9% identity) from that of
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD0709BFD64FB7070E8" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[1155,1235,724,743]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD0709BFD64FB7070E8" bold="true" box="[1155,1235,724,743]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
.
|
|||
|
|
</paragraph>
|
|||
|
|
<paragraph id="8BD936B5FF9DFFD0774BFD40FB467615" blockId="5.[818,1488,585,1720]" pageId="5" pageNumber="6">
|
|||
|
|
The new 1504-bp-long sequence of the partial 28S rRNA gene that was assigned to
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD077D2FCBCFBBB7110" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[970,1048,780,799]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD077D2FCBCFBBB7110" bold="true" box="[970,1048,780,799]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank no.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD070BBFCBCFB667110" box="[1187,1221,780,799]" pageId="5" pageNumber="6">MF</collectionCode>
|
|||
|
|
946610) differed at only one of 1474 overlapping positions (
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD07047FC98FBCE7134" box="[1119,1133,808,827]" name="Tavera, Department of Geology and Geophysics" pageId="5" pageNumber="6">T</collectionCode>
|
|||
|
|
instead of A at position 445 of new sequence; 99.9% identity) from GenBank sequence JQ733513 of the unnamed species from the great cormorant. As regards other species, the most similar ones were
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07054FCCCFB1F7180" box="[1100,1212,892,911]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="columbae">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07054FCCCFB1F7180" bold="true" box="[1100,1212,892,911]" italics="true" pageId="5" pageNumber="6">S. columbae</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank nos.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD07147FCCCFA257180" box="[1375,1414,892,911]" country="0" name="Hastings Museum" pageId="5" pageNumber="6">HM</collectionCode>
|
|||
|
|
125053, GU253887; 99.5% identity),
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07047FC28FB1D71A4" box="[1119,1214,920,939]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="corvusi">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07047FC28FB1D71A4" bold="true" box="[1119,1214,920,939]" italics="true" pageId="5" pageNumber="6">S. corvusi</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank no. JN256118; 99.5%),
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD0779AFC04FC4671C8" box="[898,997,948,967]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="wobeseri">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD0779AFC04FC4671C8" bold="true" box="[898,997,948,967]" italics="true" pageId="5" pageNumber="6">S. wobeseri</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank nos.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD07062FC04FB3F71C8" box="[1146,1180,948,967]" pageId="5" pageNumber="6">GQ</collectionCode>
|
|||
|
|
922887, GQ922888,
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD07147FC04FA2671C8" box="[1375,1413,948,967]" country="0" name="Hastings Museum" pageId="5" pageNumber="6">HM</collectionCode>
|
|||
|
|
159420,
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD0772AFC60FCEE71EC" box="[818,845,976,995]" pageId="5" pageNumber="6">EF</collectionCode>
|
|||
|
|
079886; 99.0%), and
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD0703CFC60FBDE71EC" box="[1060,1149,976,995]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="turdusi">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD0703CFC60FBDE71EC" bold="true" box="[1060,1149,976,995]" italics="true" pageId="5" pageNumber="6">S. turdusi</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank no. JF975682; 99.0%), whereas many other species with birds or carnivores as intermediate hosts showed identities between 98 and 99%.
|
|||
|
|
</paragraph>
|
|||
|
|
<paragraph id="8BD936B5FF9DFFD0774BFB93FA8674B7" blockId="5.[818,1488,585,1720]" pageId="5" pageNumber="6">
|
|||
|
|
As regards the ITS1region, a total of eight clones of
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07153FB93FA387639" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[1355,1435,1059,1078]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07153FB93FA387639" bold="true" box="[1355,1435,1059,1078]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
from one isolate were processed and sequenced (GenBank nos.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD0772AFBEBFCF07661" box="[818,851,1115,1134]" pageId="5" pageNumber="6">MF</collectionCode>
|
|||
|
|
946589–MF946596;
|
|||
|
|
<tableCitation id="C6E4030EFF9DFFD0700EFBEBFBFC7661" box="[1046,1119,1115,1134]" captionStart="Table 1" captionStartId="5.[100,145,162,177]" captionText="Table 1 Overview of the molecular characterization at different markers of ten DNA isolates (Ha1.1–Ha1.10) from the intestinal mucosa of a white-tailed sea eagle and the Sarcocystis species identified in each isolate. The GenBank accession numbers of sequences obtained from particular isolates are given in parenthesis behind the isolates of origin." pageId="5" pageNumber="6">Table 1</tableCitation>
|
|||
|
|
). The full-length sequences, including both primers, were 1083 or 1085 bp long, of which the ITS1 region comprised 828 or 830 bp, and the flanking portions of the 18S and
|
|||
|
|
<geoCoordinate id="EE525072FF9DFFD071BEFB23FA6C76A9" box="[1446,1487,1171,1190]" degrees="5.8" direction="south" orientation="latitude" pageId="5" pageNumber="6" precision="5555" value="-5.8">5.8S</geoCoordinate>
|
|||
|
|
rRNA genes, 137 and 118 bp, respectively. The eight clones differed from each other at 1–9 nucleotide positions in the ITS1 region (98.9–99.9% identity within the ITS1 region; 99.2–99.9% identity between full-length sequences). These differences were mainly due to substitutions (single nucleotide polymorphisms, SNPs), but one clone (GenBank no.
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD077A0FA8AFC797742" box="[952,986,1338,1357]" pageId="5" pageNumber="6">MF</collectionCode>
|
|||
|
|
946596) differed from the seven others in having two deletions. Similarly, at the positions displaying substitutions, only one sequence deviated from the others. Hence, these variations might have been due to polymerase errors during the PCR amplification prior to cloning. The eight clones differed from GenBank sequence JQ733513 of the unnamed species from the great cormorant, which comprised the ITS1 region only, at 11–15 of 830 positions (98.2–98.7% identity). At 11 of these positions, all the eight new sequences differed from that sequence. With respect to other species, the ITS1 region of
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07166F9A9FA6C7423" authorityName="Gjerde & Vikøren & Hamnes" authorityYear="2018" box="[1406,1487,1561,1580]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="halieti">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07166F9A9FA6C7423" bold="true" box="[1406,1487,1561,1580]" italics="true" pageId="5" pageNumber="6">S. halieti</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
showed the highest identity with this region of an unnamed
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07175F985FCEE746B" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="undetermined">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07175F985FA6C7447" bold="true" box="[1389,1487,1589,1608]" italics="true" pageId="5" pageNumber="6">Sarcocystis</emphasis>
|
|||
|
|
sp.
|
|||
|
|
</taxonomicName>
|
|||
|
|
from the intestine of
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07006F9E1FB23746B" baseAuthorityName="Bonaparte" baseAuthorityYear="1828" box="[1054,1152,1617,1636]" class="Aves" family="Accipitridae" genus="Accipiter" kingdom="Animalia" order="Accipitriformes" pageId="5" pageNumber="6" phylum="Chordata" rank="species" species="cooperii">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07006F9E1FB23746B" bold="true" box="[1054,1152,1617,1636]" italics="true" pageId="5" pageNumber="6">A. cooperii</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank no. KY348755; 93%),
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD071A6F9E1FC25748F" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="columbae">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD071A6F9E1FC25748F" bold="true" italics="true" pageId="5" pageNumber="6">S. columbae</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank nos. GU253885,
|
|||
|
|
<collectionCode id="ED77AE70FF9DFFD0708FF9DDFB1E748F" box="[1175,1213,1645,1664]" country="0" name="Hastings Museum" pageId="5" pageNumber="6">HM</collectionCode>
|
|||
|
|
125052; 92%) and
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD0716FF9DDFA6C748F" box="[1399,1487,1645,1664]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="corvusi">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD0716FF9DDFA6C748F" bold="true" box="[1399,1487,1645,1664]" italics="true" pageId="5" pageNumber="6">S. corvusi</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
(GenBank no. JN256119; 92%), followed by identities of about 84% with several sequences of
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD07033F915FB2974B7" box="[1067,1162,1701,1720]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="calchasi">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD07033F915FB2974B7" bold="true" box="[1067,1162,1701,1720]" italics="true" pageId="5" pageNumber="6">S. calchasi</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
and
|
|||
|
|
<taxonomicName id="4C664D36FF9DFFD070A3F915FABC74B7" box="[1211,1311,1701,1720]" family="Sarcocystidae" genus="Sarcocystis" kingdom="Chromista" order="Eucoccidiida" pageId="5" pageNumber="6" phylum="Miozoa" rank="species" species="wobeseri">
|
|||
|
|
<emphasis id="B912EAA7FF9DFFD070A3F915FABC74B7" bold="true" box="[1211,1311,1701,1720]" italics="true" pageId="5" pageNumber="6">S. wobeseri</emphasis>
|
|||
|
|
</taxonomicName>
|
|||
|
|
.
|
|||
|
|
</paragraph>
|
|||
|
|
</subSubSection>
|
|||
|
|
</treatment>
|
|||
|
|
</document>
|