135 lines
14 KiB
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135 lines
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<document id="D3525FE9399B8A6E31FEBC81909C26B9" ID-DOI="10.1016/j.phytochem.2021.112852" ID-ISSN="1873-3700" ID-Zenodo-Dep="8259265" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1692308480071" checkinUser="felipe" docAuthor="Pu, Xue-Juan, Hu, Qian-Yi, Li, Su-Su, Li, Guo-Hong & Zhao, Pei-Ji" docDate="2021" docId="F77187DEFFA88653100FFEAEA257FB0D" docLanguage="en" docName="Phytochemistry.189.112852.pdf" docOrigin="Phytochemistry (112852) 189" docSource="http://dx.doi.org/10.1016/j.phytochem.2021.112852" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Stereum hirsutum" docType="treatment" docVersion="3" lastPageNumber="3" masterDocId="0B48FFA6FFAA8651106BFFAAA040FFA2" masterDocTitle="Sesquiterpenoids and their quaternary ammonium hybrids from the mycelium of mushroom Stereum hirsutum by medium optimization" masterLastPageNumber="8" masterPageNumber="1" pageNumber="3" updateTime="1692728562026" updateUser="julia">
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<mods:title id="73B3369DA5A6A5E891701516F9BA9224">Sesquiterpenoids and their quaternary ammonium hybrids from the mycelium of mushroom Stereum hirsutum by medium optimization</mods:title>
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<mods:namePart id="E5C91293E7C20E8E8D1DF70BE4D983CA">Pu, Xue-Juan</mods:namePart>
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<mods:affiliation id="2D06EB8B3261E580EB23AEABC874EE21">*</mods:affiliation>
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<mods:namePart id="E0CF5E765C809556AFECB05E033D0B3D">Hu, Qian-Yi</mods:namePart>
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</mods:name>
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<mods:name id="9E10185CA3FE3E1C0E1F8186AB065468" type="personal">
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<mods:namePart id="CDF859FE48824E0EC8C0D890BB81A6BC">Li, Su-Su</mods:namePart>
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<mods:namePart id="3F4C3B18DE605329A83D4BE69BD33B41">Li, Guo-Hong</mods:namePart>
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<mods:name id="3F3EA0C7615DE34171C250E38608556E" type="personal">
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<mods:namePart id="6EA43F3F0B7A9F50522D43EA011042F7">Zhao, Pei-Ji</mods:namePart>
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<mods:typeOfResource id="9553567F1270CC9E4BB5E50D183BFD05">text</mods:typeOfResource>
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<mods:title id="51C6CCA89F43C819D48560E6B0304DFB">Phytochemistry</mods:title>
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<mods:part id="1B91774D8BFB875E5056B6962746DEE3">
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<mods:date id="36F22E193DB737DDD1D56FC99331AC6C">2021</mods:date>
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<mods:detail id="E2BA1A75410820BABAD273DA478EAC6A" type="series">
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<mods:title id="80475F8268F28ED5696AF0BBDD1E95CA">112852</mods:title>
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</mods:detail>
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<mods:number id="807071F359FCED44E55F1C8A71D3BB8C">2021-09-30</mods:number>
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<mods:number id="B2719C2B163D9E58C22FAABE02838DA3">189</mods:number>
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<mods:start id="82FA202E5600318B5E4D26DFEEE2E3D1">1</mods:start>
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<mods:url id="90BF897D12FF1BC4D83A4EFB213EFD48">http://dx.doi.org/10.1016/j.phytochem.2021.112852</mods:url>
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<mods:classification id="7F7C3B9287E7AB15C2105373E0019E5E">journal article</mods:classification>
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<mods:identifier id="281766B82FAC9D1623A36363348BF984" type="DOI">10.1016/j.phytochem.2021.112852</mods:identifier>
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<mods:identifier id="CEA8DDBEA9F1804EB9AC23F64908C8D9" type="ISSN">1873-3700</mods:identifier>
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<mods:identifier id="6747A672974ADBFE7043636C9E8C2516" type="Zenodo-Dep">8259265</mods:identifier>
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</mods:mods>
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<treatment id="F77187DEFFA88653100FFEAEA257FB0D" LSID="urn:lsid:plazi:treatment:F77187DEFFA88653100FFEAEA257FB0D" httpUri="http://treatment.plazi.org/id/F77187DEFFA88653100FFEAEA257FB0D" lastPageNumber="3" pageId="2" pageNumber="3">
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<subSubSection id="37C26543FFA88653100FFEAEA176FE90" pageId="2" pageNumber="3" type="nomenclature">
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<paragraph id="7F6736C8FFA88653100FFEAEA176FE90" blockId="2.[100,660,259,279]" lastBlockId="2.[100,310,287,306]" pageId="2" pageNumber="3">
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<emphasis id="4DACEADAFFA88653100FFEAEA176FE90" bold="true" italics="true" pageId="2" pageNumber="3">
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<heading id="242F81A4FFA88653100FFEAEA2D4FEB4" bold="true" box="[100,660,259,279]" fontSize="36" level="1" pageId="2" pageNumber="3" reason="1">
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2.1. LC-MS profiling of
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<taxonomicName id="B8D84D4BFFA886531129FEA9A1E8FEB4" ID-CoL="6ZQZP" ID-ENA="2818322" authority="(Willd.)" box="[322,424,259,278]" class="Agaricomycetes" family="Stereaceae" genus="Stereum" kingdom="Fungi" order="Russulales" pageId="2" pageNumber="3" phylum="Basidiomycota" rank="species" species="hirsutum">S. hirsutum</taxonomicName>
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FP-91666 under different
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</heading>
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<heading id="242F81A4FFA88653100FFEB5A176FE90" box="[100,310,287,306]" fontSize="8" level="3" pageId="2" pageNumber="3" reason="8">fermentation conditions</heading>
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</emphasis>
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</paragraph>
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</subSubSection>
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<subSubSection id="37C26543FFA8865310EFFEFDA257FB0D" pageId="2" pageNumber="3" type="description">
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<paragraph id="7F6736C8FFA8865310EFFEFDA2DCFD53" blockId="2.[100,770,343,1200]" pageId="2" pageNumber="3">
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Microorganisms are susceptible to culture conditions, such as medium constituents, temperature, medium status, and so on. Altering these factors can lead to changes in the chemical diversity of specialised metabolites. In this study,
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<taxonomicName id="B8D84D4BFFA886531130FE01A180FE1C" authority="(Willd.)" box="[347,448,427,446]" class="Agaricomycetes" family="Stereaceae" genus="Stereum" kingdom="Fungi" order="Russulales" pageId="2" pageNumber="3" phylum="Basidiomycota" rank="species" species="hirsutum">
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<emphasis id="4DACEADAFFA886531130FE01A180FE1C" bold="true" box="[347,448,427,446]" italics="true" pageId="2" pageNumber="3">S. hirsutum</emphasis>
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</taxonomicName>
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FP-91666 was grown in
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<specimenCount id="69DEFD41FFA8865312C3FE01A342FE1C" box="[680,770,427,446]" pageId="2" pageNumber="3" type="generic" typeStatus="types">five types</specimenCount>
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of media (CMB, GPY, PDB, WG, and YMG) together with their solid condition (CMA, GPYA, PDA, WGA, and YMGA), and then LC-MS analyses of the extracts were used to reveal the metabolite diversity of
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<taxonomicName id="B8D84D4BFFA88653100FFDB0A08CFD8F" authority="(Willd.)" box="[100,204,538,557]" class="Agaricomycetes" family="Stereaceae" genus="Stereum" kingdom="Fungi" order="Russulales" pageId="2" pageNumber="3" phylum="Basidiomycota" rank="species" species="hirsutum">
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<emphasis id="4DACEADAFFA88653100FFDB0A08CFD8F" bold="true" box="[100,204,538,557]" italics="true" pageId="2" pageNumber="3">S. hirsutum</emphasis>
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</taxonomicName>
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FP-91666 and obtain as many undescribed metabolites as possible. The total ion chromatogram (TIC) profiles of the extracts are almost the same for the five solid media (
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<figureCitation id="E7E32A4DFFA886531195FDF8A208FDC4" box="[510,584,594,614]" captionStart="Fig" captionStartId="1.[421,451,1964,1981]" captionTargetBox="[264,1323,472,1936]" captionTargetId="figure-233@1.[263,1324,471,1937]" captionTargetPageId="1" captionText="Fig. 1. The LC-MS profiles of extracts for S. hirsutum FP-91666 cultures on five media." figureDoi="http://doi.org/10.5281/zenodo.8259267" httpUri="https://zenodo.org/record/8259267/files/figure.png" pageId="2" pageNumber="3">Fig. 1A</figureCitation>
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), and the retention times show that these metabolites are mainly non- or low polar metabolites. However, the TIC profiles of the liquid cultural extracts (
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<figureCitation id="E7E32A4DFFA886531007FD0CA0F4FD1B" box="[108,180,678,697]" captionStart="Fig" captionStartId="1.[421,451,1964,1981]" captionTargetBox="[264,1323,472,1936]" captionTargetId="figure-233@1.[263,1324,471,1937]" captionTargetPageId="1" captionText="Fig. 1. The LC-MS profiles of extracts for S. hirsutum FP-91666 cultures on five media." figureDoi="http://doi.org/10.5281/zenodo.8259267" httpUri="https://zenodo.org/record/8259267/files/figure.png" pageId="2" pageNumber="3">Fig. 1B</figureCitation>
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) show that the metabolites from the WG extract are more abundant, and the retention times of the metabolites reveal that the metabolites are mainly medium or highly polar compounds.
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</paragraph>
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<paragraph id="7F6736C8FFA8865310EFFD50A257FB0D" blockId="2.[100,770,343,1200]" pageId="2" pageNumber="3">
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In order to further understand the metabolites in the extract chromatograms, we selected ion monitoring mode (SIM) to analyze the LC-MS chromatograms. The products of the strain cultivated on WGB medium were more diverse and abundant than those of strains grown on the other culture media (
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<figureCitation id="E7E32A4DFFA88653113FFCC3A1DBFCDE" box="[340,411,873,892]" captionStart="Fig" captionStartId="1.[421,451,1964,1981]" captionTargetBox="[264,1323,472,1936]" captionTargetId="figure-233@1.[263,1324,471,1937]" captionTargetPageId="1" captionText="Fig. 1. The LC-MS profiles of extracts for S. hirsutum FP-91666 cultures on five media." figureDoi="http://doi.org/10.5281/zenodo.8259267" httpUri="https://zenodo.org/record/8259267/files/figure.png" pageId="2" pageNumber="3">Fig. 1C</figureCitation>
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). According to the SIM profiles, some highly enriched ESIMS peaks were found in LC-MS chromatograms of extracts from
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<taxonomicName id="B8D84D4BFFA88653108FFC0BA108FC16" authority="(Willd.)" box="[228,328,929,948]" class="Agaricomycetes" family="Stereaceae" genus="Stereum" kingdom="Fungi" order="Russulales" pageId="2" pageNumber="3" phylum="Basidiomycota" rank="species" species="hirsutum">
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<emphasis id="4DACEADAFFA88653108FFC0BA108FC16" bold="true" box="[228,328,929,948]" italics="true" pageId="2" pageNumber="3">S. hirsutum</emphasis>
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</taxonomicName>
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FP-91666 on WGA medium, such as sterostrein Q
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<emphasis id="4DACEADAFFA886531017FC17A0E1FC72" bold="true" box="[124,161,957,976]" italics="true" pageId="2" pageNumber="3">m/z</emphasis>
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244 [M + H]
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<superScript id="88AD9B80FFA886531142FC13A173FC67" attach="none" box="[297,307,953,965]" fontSize="6" pageId="2" pageNumber="3">+</superScript>
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, stereumamide D
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<emphasis id="4DACEADAFFA88653118CFC17A24BFC72" bold="true" box="[487,523,957,976]" italics="true" pageId="2" pageNumber="3">m/z</emphasis>
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431 [M + H]
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<superScript id="88AD9B80FFA8865312FFFC13A2DEFC67" attach="none" box="[660,670,953,965]" fontSize="6" pageId="2" pageNumber="3">+</superScript>
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, and stereumamides B and H
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<emphasis id="4DACEADAFFA886531140FC73A110FC4E" bold="true" box="[299,336,985,1004]" italics="true" pageId="2" pageNumber="3">m/z</emphasis>
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358 [M + H]
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<superScript id="88AD9B80FFA8865311BFFC7FA19EFC43" attach="none" box="[468,478,981,993]" fontSize="6" pageId="2" pageNumber="3">+</superScript>
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. Several unknown peaks were also present in the product spectra of the extracts. A positive SIM peak at
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<emphasis id="4DACEADAFFA88653100FFBBBA0C9FB86" bold="true" box="[100,137,1041,1060]" italics="true" pageId="2" pageNumber="3">m/z</emphasis>
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392 was present in the product spectra (
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<figureCitation id="E7E32A4DFFA886531245FBBBA236FB86" box="[558,630,1041,1060]" captionStart="Fig" captionStartId="1.[421,451,1964,1981]" captionTargetBox="[264,1323,472,1936]" captionTargetId="figure-233@1.[263,1324,471,1937]" captionTargetPageId="1" captionText="Fig. 1. The LC-MS profiles of extracts for S. hirsutum FP-91666 cultures on five media." figureDoi="http://doi.org/10.5281/zenodo.8259267" httpUri="https://zenodo.org/record/8259267/files/figure.png" pageId="2" pageNumber="3">Fig. 1C</figureCitation>
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) of the strain cultured on the WGB medium and the relative strength of the peak was strong (NL:
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<geoCoordinate id="1AEC500FFFA8865310B7FBE2A158FBF9" box="[220,280,1096,1115]" degrees="2.16" direction="east" orientation="longitude" pageId="2" pageNumber="3" precision="555" value="2.16">2.16E</geoCoordinate>
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8). Other positive SIM peaks at
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<emphasis id="4DACEADAFFA88653123FFBE2A239FBF9" bold="true" box="[596,633,1096,1115]" italics="true" pageId="2" pageNumber="3">m/z</emphasis>
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374 and 290 (
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<figureCitation id="E7E32A4DFFA886531007FBCEA0F2FBD5" box="[108,178,1124,1143]" captionStart="Fig" captionStartId="1.[421,451,1964,1981]" captionTargetBox="[264,1323,472,1936]" captionTargetId="figure-233@1.[263,1324,471,1937]" captionTargetPageId="1" captionText="Fig. 1. The LC-MS profiles of extracts for S. hirsutum FP-91666 cultures on five media." figureDoi="http://doi.org/10.5281/zenodo.8259267" httpUri="https://zenodo.org/record/8259267/files/figure.png" pageId="2" pageNumber="3">Fig. 1C</figureCitation>
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) were also observed in the LC-MS profiles. Based on these results, the WGB medium was selected for the fermentation of
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<taxonomicName id="B8D84D4BFFA8865312F6FB2AA342FB31" authority="(Willd.)" box="[669,770,1152,1171]" class="Agaricomycetes" family="Stereaceae" genus="Stereum" kingdom="Fungi" order="Russulales" pageId="2" pageNumber="3" phylum="Basidiomycota" rank="species" species="hirsutum">
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<emphasis id="4DACEADAFFA8865312F6FB2AA342FB31" bold="true" box="[669,770,1152,1171]" italics="true" pageId="2" pageNumber="3">S. hirsutum</emphasis>
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</taxonomicName>
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FP-91666 to explore undescribed metabolites.
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</paragraph>
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</subSubSection>
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</treatment>
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</document>
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