131 lines
8.8 KiB
Turtle
131 lines
8.8 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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@prefix xlink: <http://www.w3.org/1999/xlink/> .
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<http://treatment.plazi.org/id/03A98783675CFFF8140D8AC9FD5AFBA7>
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cito:cites <http://dx.doi.org/10.5281/zenodo.10994457>, <http://dx.doi.org/10.5281/zenodo.10994459>, <http://dx.doi.org/10.5281/zenodo.10994461>, <http://dx.doi.org/10.5281/zenodo.10994463>, <http://dx.doi.org/10.5281/zenodo.10994465>, <http://dx.doi.org/10.5281/zenodo.10994467>, <http://dx.doi.org/10.5281/zenodo.10994469> ;
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dc:creator "Ikeda, Risa; Kurokawa, Miki; Murai, Momoka; Saito, Noboru; Ando, Motonori" ;
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dc:title "Raphidiophrys contractilis Kinoshita, Suzaki, Shigenaka & Sugiyama 1995" ;
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trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Chromista/Raphidiophrys_contractilis_Kinoshita_1995> ;
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trt:publishedIn <http://dx.doi.org/10.4467/16890027ap.20.001.12157> ;
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a trt:Treatment .
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<http://dx.doi.org/10.4467/16890027ap.20.001.12157>
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bibo:endPage "12" ;
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bibo:issue "1" ;
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bibo:journal "Acta Protozoologica" ;
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bibo:startPage "1" ;
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bibo:volume "59" ;
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dc:creator "Ikeda, Risa; Kurokawa, Miki; Murai, Momoka; Saito, Noboru; Ando, Motonori" ;
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dc:date "2020" ;
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dc:title "Immunocytochemical Analysis of α-Tubulin Distribution Before and After Rapid Axopodial Contraction in the Centrohelid Raphidocystis contractilis" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.10994457>, <http://dx.doi.org/10.5281/zenodo.10994459>, <http://dx.doi.org/10.5281/zenodo.10994461>, <http://dx.doi.org/10.5281/zenodo.10994463>, <http://dx.doi.org/10.5281/zenodo.10994465>, <http://dx.doi.org/10.5281/zenodo.10994467>, <http://dx.doi.org/10.5281/zenodo.10994469> ;
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a fabio:JournalArticle .
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<http://taxon-concept.plazi.org/id/Chromista/Raphidiophrys_contractilis_Kinoshita_1995>
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dwc:authority "Kinoshita, Suzaki, Shigenaka & Sugiyama, 1995" ;
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dwc:authorityName "Kinoshita, Suzaki, Shigenaka & Sugiyama" ;
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dwc:authorityYear "1995" ;
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dwc:family "Raphidiophryidae" ;
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dwc:genus "Raphidiophrys" ;
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dwc:kingdom "Chromista" ;
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dwc:order "Centrohelida" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "species" ;
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dwc:scientificNameAuthorship "Kinoshita, Suzaki, Shigenaka & Sugiyama, 1995" ;
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dwc:species "contractilis" ;
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trt:hasTaxonName <http://taxon-name.plazi.org/id/Chromista/Raphidiophrys_contractilis> ;
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a dwcFP:TaxonConcept .
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<http://taxon-name.plazi.org/id/Chromista/Raphidiophrys_contractilis>
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dwc:family "Raphidiophryidae" ;
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dwc:genus "Raphidiophrys" ;
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dwc:kingdom "Chromista" ;
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dwc:order "Centrohelida" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "species" ;
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dwc:species "contractilis" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Raphidiophrys> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Chromista/Raphidiophrys>
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dwc:family "Raphidiophryidae" ;
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dwc:genus "Raphidiophrys" ;
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dwc:kingdom "Chromista" ;
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dwc:order "Centrohelida" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Raphidiophryidae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Chromista/Raphidiophryidae>
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dwc:family "Raphidiophryidae" ;
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dwc:kingdom "Chromista" ;
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dwc:order "Centrohelida" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Centrohelida> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Chromista/Centrohelida>
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dwc:kingdom "Chromista" ;
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dwc:order "Centrohelida" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Heliozoa> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Chromista/Heliozoa>
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dwc:kingdom "Chromista" ;
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dwc:phylum "Heliozoa" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Chromista> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Chromista>
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dwc:kingdom "Chromista" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.10994457>
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dc:description "Fig. 1. (A, B) Rapid axopodial contraction induced by mechanical stimulation in R. contractilis. Images (A) before and (B) after rapid axopodial contraction. Arrowheads indicate kinetocysts. Note the synchronized retraction of all axopodia and the apparent increases in the widths of contracted axopodia relative to the features observed before the onset of axopodial contraction (an arrow). Scale bar: 20 µm (A, B)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994457/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994459>
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dc:description "Fig. 2. (A, B) Fine structures associated with axopodial microtubules. (A) Centroplast in the center of the cell. (B) Cross-section of an axopo- dium in the peripheral region of the cell. Note that bundles of microtubules radiate from the centroplast (arrowheads) and that the axopodium comprises six microtubules. Scale bars: 500 nm (A), 100 nm (B)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994459/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994461>
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dc:description "Fig. 3. Schematic illustration of the experimental setup. The fixative is injected into the cell suspension via a micro flow-through chamber with a syringe pump. Next, the cells are fixed by injecting the fixative at a rate below the threshold required for inducing rapid contraction. The cells are then observed under a microscope." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994461/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994463>
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dc:description "Fig. 4. (A–D) Evaluation of the maintenance of the original lengths of axopodia in R. contractilis using different combinations of fixative and buffer. Cells were fixed with (A) 4% paraformaldehyde in phosphate buffer, (B) 4% paraformaldehyde in PHEM, (C) 0.2% glutaralde- hyde in phosphate buffer, or (D) 0.2% glutaraldehyde in PHEM. Note that only fixation with 0.2% glutaraldehyde in PHEM maintained the axopodial length. Scale bar: 20 µm." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994463/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994465>
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dc:description "Fig. 5. (A, B) The distribution of α-tubulin in the cell before rapid axopodial contraction. (A) Low-magnification images of a cell without the induction of rapid axopodial contraction.A projection image of the whole cell was constructed from 192 optical sections obtained at 0.5 µm intervals (left); the corresponding light micrograph is also shown (right). (B) High-magnification images of the same cell shown in (A). A projection image of the equatorial plane of the cell body constructed from 5 optical sections taken at 0.5 µm intervals, and the correspond- ing light micrograph. The asterisk indicates a centroplast. Note that positive signals were detected along the fully extended axopodia. Scale bars: 20 μm (A), 10 μm (B)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994465/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994467>
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dc:description "Fig. 6. Confocal analysis of α-tubulin-immunolabeled extended axopodia. Fine reconstructed projection image from 161 optical sections taken at a 0.05 µm (top) and the corresponding light micrograph (bottom). Note the regions of relatively low fluorescence (arrowheads) did not correspond to the location of kinetocysts (arrows). Scale bar: 10 μm." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994467/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10994469>
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dc:description "Fig. 7. (A, B) The distribution of α-tubulin in the cell after rapid axopodial contraction. (A) Low-magnification images of the cell after the induction of rapid axopodial contraction. A projection image of the whole cell constructed from 87 optical sections taken at a 0.5 µm interval (left) and the corresponding light micrograph (right). (B) High-magnification images of the same cell shown in (A). A projection image of the equatorial plane of the cell body constructed from 5 optical sections taken at a 0.5 µm interval (left), and the corresponding light micrograph (right). Note that the positive signals in the distal parts of the axopodia have a branched appearance (arrowheads). Scale bars: 20 μm (A), 10 μm (B)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10994469/files/figure.png> ;
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a fabio:Figure .
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