127 lines
9.5 KiB
Turtle
127 lines
9.5 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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<http://treatment.plazi.org/id/039ADC33D904FF897C6C8ACDFABDFB7A>
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cito:cites <http://dx.doi.org/10.5281/zenodo.10530253>, <http://dx.doi.org/10.5281/zenodo.10530255>, <http://dx.doi.org/10.5281/zenodo.10530249>, <http://dx.doi.org/10.5281/zenodo.10530257>, <http://dx.doi.org/10.5281/zenodo.10530259>, <http://dx.doi.org/10.5281/zenodo.10530251>, <http://dx.doi.org/10.5281/zenodo.10530261> ;
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dc:creator "Ricigliano, Vincent; Kumar, Santosh; Kinison, Scott; Brooks, Christopher; Nybo, S. Eric; Chappell, Joe; Howarth, Dianella G." ;
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dc:title "Valeriana officinalis subsp. hairy" ;
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trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Plantae/Valeriana_officinalis_Linnaeus_> ;
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trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2016.02.011> ;
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a trt:Treatment .
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<http://dx.doi.org/10.1016/j.phytochem.2016.02.011>
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bibo:endPage "53" ;
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bibo:journal "Phytochemistry" ;
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bibo:pubDate "2016-05-31" ;
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bibo:startPage "43" ;
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bibo:volume "125" ;
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dc:creator "Ricigliano, Vincent; Kumar, Santosh; Kinison, Scott; Brooks, Christopher; Nybo, S. Eric; Chappell, Joe; Howarth, Dianella G." ;
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dc:date "2016" ;
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dc:title "Regulation of sesquiterpenoid metabolism in recombinant and elicited Valeriana officinalis hairy roots" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.10530253>, <http://dx.doi.org/10.5281/zenodo.10530255>, <http://dx.doi.org/10.5281/zenodo.10530249>, <http://dx.doi.org/10.5281/zenodo.10530257>, <http://dx.doi.org/10.5281/zenodo.10530259>, <http://dx.doi.org/10.5281/zenodo.10530251>, <http://dx.doi.org/10.5281/zenodo.10530261> ;
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a fabio:JournalArticle .
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<http://taxon-concept.plazi.org/id/Plantae/Valeriana_officinalis_Linnaeus_>
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dwc:authority "L." ;
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dwc:class "Magnoliopsida" ;
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dwc:family "Caprifoliaceae" ;
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dwc:genus "Valeriana" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Dipsacales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "species" ;
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dwc:scientificNameAuthorship "Linnaeus" ;
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dwc:species "officinalis" ;
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rdfs:seeAlso <https://www.catalogueoflife.org/data/taxon/7FCMV> ;
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trt:hasTaxonName <http://taxon-name.plazi.org/id/Plantae/Valeriana_officinalis> ;
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a dwcFP:TaxonConcept .
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<http://taxon-name.plazi.org/id/Plantae/Valeriana_officinalis>
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dwc:class "Magnoliopsida" ;
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dwc:family "Caprifoliaceae" ;
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dwc:genus "Valeriana" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Dipsacales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "species" ;
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dwc:species "officinalis" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Valeriana> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Valeriana>
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dwc:genus "Valeriana" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Caprifoliaceae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Caprifoliaceae>
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dwc:family "Caprifoliaceae" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Dipsacales> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Dipsacales>
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dwc:order "Dipsacales" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Magnoliopsida> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Magnoliopsida>
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dwc:class "Magnoliopsida" ;
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dwc:rank "class" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Tracheophyta> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Tracheophyta>
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dwc:phylum "Tracheophyta" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae>
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dwc:kingdom "Plantae" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.10530253>
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dc:description "Fig. 6. Schematic diagrams of the plasmids used for A. rhizogenes-mediated binary vector co-transformation of V. officinalis hairy roots. (a) A. rhizogenes root-inducing plasmid pRi15834. The T-region comprises the aux and rol loci, which occur on separate T-DNAs. Transfer and integration of the T-region is mediated by the vir locus, whose gene products act in trans on 25-bp border repeat sequences to the right (RB) and left (LB) of T-DNA domains.(b) pBin derived binary vector Vo257.The T-DNA contains the selection marker neomycin phosphotranferase (NPTII), which is flanked by the nopaline synthase promoter (NOS-P) and nopaline synthase terminator (NOS-T). The CaMV35s promoter (35S-P) drives constitutive expression of the downstream gene of interest (GOI)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530253/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530255>
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dc:description "Fig. 7. Establishment of binary vector co-transformation conditions and heterologous gene expression. (a) Kanamycin sensitivity assay of pRi15834 control hairy roots. Root tips were inoculated to MS medium containing a range of kanamycin concentrations (0, 20, 40, 60, 80 mg/L) and qualified after 30 days. (b) GUS histochemical staining of cotransformed hairy roots. The lines harbored the pVo257 T-DNA, which contained a GUS-intron overexpression cassette, or the empty vector control (pVo257 EV). The three independent GUS lines exhibited varying degrees of staining, whereas no staining occurred in the empty vector line (C) Magnified visualization of GUS expression along the root axis including individual root hairs." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530255/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530249>
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dc:description "Fig. 4. Quantitati-ve real time polymerase chain reaction (qRT-PCR) expression profiling of VoFPS, VoVDS, VoGCS and CYP71D442 transcripts in wild-type V. officinalis plant tissues and hairy roots. (a) Relative transcript levels in leaf, stem, young root, mature root wild-type tissues and hairy root. (b) Time course of transcript levels in methyl jasmonate (MeJA) treated hairy root cultures. Actin expression levels were used as a reference for normalization. Three biological and technical replicates were used to certify the accuracy of the results and to calculate the standard error for each sample. For time course, asterisks indicate statistical significance in comparison to 0 h control assessed by one-way ANOVA (**, P <0.01; *, P <0.05)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530249/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530257>
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dc:description "Fig. 8. Representative phenotypes of binary vector co-transformed V. officinalis hairy root lines grown on solid media and in liquid cultures. Showing empty vector control (pVo257 EV) compared to lines generated for overexpression of the VoFPS (VoFPS-1) and VoVDS (VoVDS-3) genes." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530257/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530259>
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dc:description "Fig. 9. Transcriptional profiling of recombinant V.officinalis hairy root lines including vector control and three independent lines overexpressing the VoFPS gene (lines 1–3) or the VoVDS gene (lines 1–3). qRT-PCR was performed following treatment with 0 µM or 100 µM MeJA for 36 h. Actin expression levels were used as a reference for normalization. Three biological and technical replicates were used to certify the accuracy of the results and to calculate the standard error for each sample. Asterisks indicate statistical significance in comparison to empty vector control assessed by one-way ANOVA (**, P <0.01; *, P <0.05). Hashes indicate statistical significance between MeJA treatments for each line as evaluated by students t-test (##, P <0.01; #, P <0.05)." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530259/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530251>
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dc:description "Fig. 5. GC–MS profile comparisons of sesquiterpenoid chemistry of hairy root cultures treated with methyl jasmonate (MeJA). Isogenic cultures were treated with 0 µM (control) (panels A, B) or 100 µM MeJA (panels C, D) and harvested for chemical analysis after 36 h. Hexane extracts prepared from the indicated cultures were analyzed directly (A, C) and after derivatization (B, D) by GC–MS. The peaks for β-caryophyllene (a), valerenadiene (b), valerenal (c) and valerenic acid (d) are annotated to facilitate comparisons." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530251/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10530261>
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dc:description "Fig. 10. Quantification of sesquiterpenoids in recombinant V. officinalis hairy root lines, including vector control and three independent lines overexpressing the VoFPS (lines 1–3) or VoVDS (lines 1–3) genes. Cultures were treated with 0 µM or 100 µM MeJA for 36 h and hexane extracts were prepared from the indicated lines from at least two samples and profiled by GC–MS prior to quantification of the indicated sesquiterpenoid relative to an internal standard." ;
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fabio:hasRepresentation <https://zenodo.org/record/10530261/files/figure.png> ;
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a fabio:Figure .
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