47 lines
5.8 KiB
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47 lines
5.8 KiB
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@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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@prefix xlink: <http://www.w3.org/1999/xlink/> .
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<http://treatment.plazi.org/id/70107B13F6538567FC8B569146A4FBE4>
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# Warning: Could not add treatment taxon because sigEpithet "" contains invalid characters ;
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cito:cites <http://dx.doi.org/10.5281/zenodo.8234974>, <http://dx.doi.org/10.5281/zenodo.8234976>, <http://dx.doi.org/10.5281/zenodo.8234978> ;
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dc:creator "Guo, Shaobin; Wang, Mingdi; Xu, Wen; Zou, Fuxian; Lin, Jingjing; Peng, Qin; Xu, Wei; Xu, Shaohua; Shi, Xianai" ;
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dc:title "Arabidopsis thaliana (L.) Heynh." ;
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trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2021.113007> ;
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a trt:Treatment .
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<http://dx.doi.org/10.1016/j.phytochem.2021.113007>
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bibo:endPage "9" ;
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bibo:journal "Phytochemistry" ;
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bibo:pubDate "2022-01-31" ;
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bibo:series "113007" ;
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bibo:startPage "1" ;
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bibo:volume "193" ;
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dc:creator "Guo, Shaobin; Wang, Mingdi; Xu, Wen; Zou, Fuxian; Lin, Jingjing; Peng, Qin; Xu, Wei; Xu, Shaohua; Shi, Xianai" ;
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dc:date "2022" ;
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dc:title "Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.8234974>, <http://dx.doi.org/10.5281/zenodo.8234976>, <http://dx.doi.org/10.5281/zenodo.8234978> ;
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a fabio:JournalArticle .
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<http://dx.doi.org/10.5281/zenodo.8234974>
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dc:description "Fig. 1. Rapid screening of plant glycosyltransferases using the LET-based-TX-TL system. We can either use long primers which contain a promoter, a ribosome binding site, and a terminator to generate expressible linear DNAs or use short primers to amplify the targeted gene fragments and then ligate them with a promoter, a ribosome binding site, a terminator, and a backbone; then another pair of primers is used to generate expressible linear DNAs. Afterward, combine TX-TL extracts, buffers, and expressible linear DNAs to start protein expression. Then this TX-TL mixture is directly added with substrates (such as quercetin) to start glycosylation reactions. Finally, UPLC-MS is used to analyze the reaction mixture to examine whether targeted products (such as isoquercitrin) are generated." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234974/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234976>
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dc:description "Fig. 2. UPLC-MS analysis of isoquercitrin converted from quercetin by AtUGTs expressed in TX-TL.(A) A glycosylation reaction catalyzed by a UDP-glucose glycosyltransferase. (B) The chromatogram of the blank sample, which only has 50% methanol solvent. (C) The chromatogram of the negative group, which has TX-TL, quercetin, UDPglucose but no additional DNA. (D) The chromatogram of the quercetin standard. (E) The chromatogram of the isoquercitrin standard. (F–O) Chromatograms of products from the catalysis of quercetin by different AtUGTs (the final concentrations of the linear DNAs used for each AtUGTs are listed below in brackets): (F) AT1G07250 (32.25 nM), (G) AT1G07260 (29.24 nM), (H) AT2G36790 (20.64 nM), (I) AT2G15480 (29.98 nM), (J) AT2G15490 (29.50 nM), (K) AT3G16520 (25.03 nM), (L) AT3G21750 (23.7 nM), (M) AT3G46660 (24.14 nM), (N) AT4G15280 (24.47 nM), and (O) AT4G34138 (22.52 nM), see Supplementary Fig. S4 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 3.38e7 in [E]) indicate the ion counts represented by “100” relative abundance. The retention time of the isoquercitrin standard is 4.0 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)" ;
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fabio:hasRepresentation <https://zenodo.org/record/8234976/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234978>
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dc:description "Fig. 3. UPLC-MS analysis of isoquercitrin converted from quercetin by ArUGTs expressed in TX-TL. (A) A glycosylation reaction catalyzed by a UDP-glucose glycosyltransferase. (B) The chromatogram of the blank sample is 50% methanol solvent. (C) The chromatogram of the negative group, which has TX- TL, quercetin, UDP-glucose but no additional DNA. (D) The chromatogram of quercetin standard. (E) The chromatogram of isoquercitrin standard. (F–K) Chromatograms of products from the catalysis of quercetin by different ArUGTs (the final concentrations of the linear DNAs used for each ArUGTs are 30 nM): (F) AR14572, (G) AR11662, (H) AR43718, (I) AR06047, (J) AR06981, and (K) AR07558, see Supplementary Fig. S5 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 2.21e7 in [E]) indicate the ion counts represented by “100” relative abundance. The retention time of the isoquercitrin standard is 4.08 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. The numbers under the protein names are the peak intensities of the product. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)" ;
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fabio:hasRepresentation <https://zenodo.org/record/8234978/files/figure.png> ;
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a fabio:Figure .
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