102 lines
6.5 KiB
Turtle
102 lines
6.5 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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@prefix xlink: <http://www.w3.org/1999/xlink/> .
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<http://treatment.plazi.org/id/8D608791DF671365FFCD2C4AFABDB246>
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cito:cites <http://dx.doi.org/10.5281/zenodo.8291748>, <http://dx.doi.org/10.5281/zenodo.8291750>, <http://dx.doi.org/10.5281/zenodo.8291742>, <http://dx.doi.org/10.5281/zenodo.8291744> ;
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dc:creator "Miller, Justin C.; Hollatz, Allison J.; Schuler, Mary A." ;
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dc:title "Camptotheca SLAS" ;
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trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2020.112626> ;
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trt:treatsTaxonName <http://taxon-name.plazi.org/id/Plantae/Camptotheca> ;
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a trt:Treatment .
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<http://dx.doi.org/10.1016/j.phytochem.2020.112626>
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bibo:endPage "13" ;
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bibo:journal "Phytochemistry" ;
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bibo:pubDate "2021-03-31" ;
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bibo:series "112626" ;
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bibo:startPage "1" ;
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bibo:volume "183" ;
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dc:creator "Miller, Justin C.; Hollatz, Allison J.; Schuler, Mary A." ;
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dc:date "2021" ;
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dc:title "P 450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.8291748>, <http://dx.doi.org/10.5281/zenodo.8291750>, <http://dx.doi.org/10.5281/zenodo.8291742>, <http://dx.doi.org/10.5281/zenodo.8291744> ;
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a fabio:JournalArticle .
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<http://taxon-name.plazi.org/id/Plantae/Camptotheca>
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dwc:class "Magnoliopsida" ;
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dwc:family "Nyssaceae" ;
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dwc:genus "Camptotheca" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Cornales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Nyssaceae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Nyssaceae>
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dwc:class "Magnoliopsida" ;
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dwc:family "Nyssaceae" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Cornales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Cornales> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Cornales>
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dwc:class "Magnoliopsida" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Cornales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Magnoliopsida> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Magnoliopsida>
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dwc:class "Magnoliopsida" ;
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dwc:kingdom "Plantae" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "class" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Tracheophyta> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Tracheophyta>
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dwc:kingdom "Plantae" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae>
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dwc:kingdom "Plantae" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.8291748>
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dc:description "Fig. 7. Molecular models of Catharanthus CYP72A1 and Camptotheca CYP72A564 and CYP72A565. (A) Backbone overlays of Catharanthus CYP72A1 and Camptotheca CYP72A564 and CYP72A565 models are shown with the alpha-carbon RMSD amongst CYP72A1, CYP72A564 and CYP72A565 depicted from green (0.0 Å) to yellow (3.0 Å) to red (4.5 Å). (B) SRS regions in CYP72A proteins shown with predicted substrate contact residues (gray fill). (C) Identical versus (D) different side chain residues predicted within 4.5 Å of loganin (aqua) docked in Catharanthus CYP72A1 (blue) and loganic acid (gray) docked in Camptotheca CYP72A564 (orange). (E) Identical versus (F) different side chain residues predicted within 4.5 Å of loganin (aqua) docked in Catharanthus CYP72A1 (blue) and loganic acid (gray) docked in Camptotheca CYP72A565 (rose)." ;
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fabio:hasRepresentation <https://zenodo.org/record/8291748/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8291750>
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dc:description "Fig. 8. Molecular models of Camptotheca CYP72A564, CYP72A565 and CYP72A730. (A) Backbone overlays of Camptotheca CYP72A730, CYP72A564 and CYP72A565 models are shown with the RMSD variance of CYP72A564 and CYP72A565 from the CYP72A730 backbone depicted in green (0.0 Å), yellow (3.0 Å) and red (4.5 Å). (B) SRS regions in CYP72A proteins shown with predicted substrate contact residues (gray fill). (C) Identical versus (D) different side chain residues predicted within 4.5 Å of loganic acid (gray) docked in Camptotheca CYP72A564 (orange) versus loganic acid (aqua) docked in CYP72A730 (magenta). (E) Identical versus (F) different side chain residues predicted within 4.5 Å of loganic acid (gray) docked in Camptotheca CYP72A565 (rose) versus loganic acid (aqua) docked in CYP72A730 (magenta)." ;
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fabio:hasRepresentation <https://zenodo.org/record/8291750/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8291742>
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dc:description "Fig. 4. LC-MS analyses of in vitro assays with purified His6-tagged CYP72A proteins reconstituted with His6-tagged CPR proteins. Reactions containing purified His6- tagged CYP72A protein, full-length His6-tagged Caa CPR1 protein (A, B) or full-length His6-tagged Caa CPR2 protein (C, D), and 250 μM loganic acid (A,C) or loganin (B,D), were incubated at 30◦C and analyzed by LC-MS as described in experimental procedures. Extracted ion chromatograms for loganic acid (m/z 375.1297), secologanic acid (m/z 373.1140), secoxyloganic acid (m/z 389.1089); loganin sodium salt (m/z +413.1418), secologanin sodium salt (m/z +411.1262), secoxyloganin (m/z 403.1246) are given with stacked chromatograms as marked." ;
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fabio:hasRepresentation <https://zenodo.org/record/8291742/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8291744>
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dc:description "Fig. 5. Area of loganic acid, loganin and products from in vitro reconstitution assays conducted with full-length Camptotheca His6-tagged CPR1. Integrated areas from LC-MS analyses of purified His6-tagged CYP72A proteins reconstituted with full-length His6-tagged Caa CPR1 are shown for no NADPH (gray) and plus NADPH (gray slashed) reactions." ;
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fabio:hasRepresentation <https://zenodo.org/record/8291744/files/figure.png> ;
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a fabio:Figure .
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