103 lines
6.2 KiB
Turtle
103 lines
6.2 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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@prefix xlink: <http://www.w3.org/1999/xlink/> .
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<http://treatment.plazi.org/id/038F146EFFCEA12F507596C46B70DF8F>
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cito:cites <http://dx.doi.org/10.5281/zenodo.11360093>, <http://dx.doi.org/10.5281/zenodo.11360095>, <http://dx.doi.org/10.5281/zenodo.11360097>, <http://dx.doi.org/10.5281/zenodo.11360099> ;
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dc:creator "Ramessur, Anusha Devi; Bothwell, John H.; Maggs, Christine A.; Gan, Sook Yee; Phang, Siew Moi" ;
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dc:title "Agrobacterium LBA" ;
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trt:publishedIn <http://dx.doi.org/10.1515/bot-2018-0028> ;
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trt:treatsTaxonName <http://taxon-name.plazi.org/id/Bacteria/Agrobacterium> ;
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a trt:Treatment .
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<http://dx.doi.org/10.1515/bot-2018-0028>
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bibo:endPage "510" ;
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bibo:issue "5" ;
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bibo:journal "Botanica Marina" ;
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bibo:pubDate "2018-09-12" ;
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bibo:series "Warsaw, Poland" ;
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bibo:startPage "499" ;
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bibo:volume "61" ;
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dc:creator "Ramessur, Anusha Devi; Bothwell, John H.; Maggs, Christine A.; Gan, Sook Yee; Phang, Siew Moi" ;
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dc:date "2018" ;
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dc:title "Agrobacterium-mediated gene delivery and transient expression in the red macroalga Chondrus crispus" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.11360093>, <http://dx.doi.org/10.5281/zenodo.11360095>, <http://dx.doi.org/10.5281/zenodo.11360097>, <http://dx.doi.org/10.5281/zenodo.11360099> ;
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a fabio:JournalArticle .
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<http://taxon-name.plazi.org/id/Bacteria/Agrobacterium>
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dwc:class "Alphaproteobacteria" ;
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dwc:family "Rhizobiaceae" ;
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dwc:genus "Agrobacterium" ;
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dwc:kingdom "Bacteria" ;
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dwc:order "Rhizobiales" ;
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dwc:phylum "Proteobacteria" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Bacteria/Rhizobiaceae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Bacteria/Rhizobiaceae>
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dwc:class "Alphaproteobacteria" ;
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dwc:family "Rhizobiaceae" ;
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dwc:kingdom "Bacteria" ;
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dwc:order "Rhizobiales" ;
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dwc:phylum "Proteobacteria" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Bacteria/Rhizobiales> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Bacteria/Rhizobiales>
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dwc:class "Alphaproteobacteria" ;
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dwc:kingdom "Bacteria" ;
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dwc:order "Rhizobiales" ;
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dwc:phylum "Proteobacteria" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Bacteria/Alphaproteobacteria> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Bacteria/Alphaproteobacteria>
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dwc:class "Alphaproteobacteria" ;
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dwc:kingdom "Bacteria" ;
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dwc:phylum "Proteobacteria" ;
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dwc:rank "class" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Bacteria/Proteobacteria> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Bacteria/Proteobacteria>
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dwc:kingdom "Bacteria" ;
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dwc:phylum "Proteobacteria" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Bacteria> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Bacteria>
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dwc:kingdom "Bacteria" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.11360093>
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dc:description "Figure 2: Intimate contact and adhesion of bacteria to wound sites of Chondrus thalli. Thalli co-cultivated for 48 h in induction medium were fixed and viewed under a scanning electron microscope: (A) control thallus incubated without bacteria showing a clean bacterium-free surface; (B) close up view of rod-shaped bacteria attached to thallus surface at wound site; (C) bacterial film at wound sites on thallus surface." ;
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fabio:hasRepresentation <https://zenodo.org/record/11360093/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.11360095>
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dc:description "Figure 3: Histochemical staining and low-power microscopic analysis of Chondrus thallus segments for transient expression of GUS 7 days after co-cultivation. (A) Pin-pricked thalli co-cultivated without bacteria, (B) pin-pricked thalli co-cultivated with untransformed LBA4404 bacteria, (C) unwounded and untransformed thalli, (D) pin-pricked thalli co-cultivated with LBA4404/pRI 910 (no GUS gene), (E) thalli transformed with LBA4404 (pCAMBIA 1301), scale bar: 1 cm." ;
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fabio:hasRepresentation <https://zenodo.org/record/11360095/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.11360097>
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dc:description "Figure 4: Effect of cultivation medium, wounding method and acetosyringone on Agrobacterium transformation efficiency. The transformation efficiencies of Chondrus thalli segments co-cultivated with pCAMBIA 1301-transformed Agrobacterium in seawater and Induction Medium (IM) were compared. Using seawater as co-cultivation medium, the wound-related treatments, namely biolistics wounding, wounding using pin-pricks and no wounding, were compared. The effect of acetosyringone (100 µM) was also studied. Data are expressed as means ± SE (n = 4). The Mann-Whitney non-parametric test was conducted to compare between two groups." ;
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fabio:hasRepresentation <https://zenodo.org/record/11360097/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.11360099>
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dc:description "Figure 5: GUS expression in Chondrus thallus segments transformed with LBA 4404/pRI 910 (Ac-GUS). (A) Surface view of control thallus not co-cultivated with Agrobacterium, (B) surface view of transformed thallus showing GUS expression at wound sites, (C) section through untransformed thallus, (D) thallus section within wounded area showing blue medullary cells at wound spots, (E) cortical cells from untransformed control thallus, (F) GUS-expressing cortical cells in transformed thallus, (G) medullary filaments from untransformed control thallus, (H) medullary filaments from transformed thallus." ;
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fabio:hasRepresentation <https://zenodo.org/record/11360099/files/figure.png> ;
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a fabio:Figure .
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