131 lines
8 KiB
Turtle
131 lines
8 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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<http://treatment.plazi.org/id/8825E57427755368A16ABD80FC378063>
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cito:cites <http://dx.doi.org/10.5281/zenodo.8234551>, <http://dx.doi.org/10.5281/zenodo.8234553>, <http://dx.doi.org/10.5281/zenodo.8234555>, <http://dx.doi.org/10.5281/zenodo.8234557>, <http://dx.doi.org/10.5281/zenodo.8234559>, <http://dx.doi.org/10.5281/zenodo.8234561> ;
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dc:creator "Rathi, Divya; Verma, Jitendra Kumar; Chakraborty, Subhra; Chakraborty, Niranjan" ;
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dc:title "Lathyrus sativus subsp. suspension L." ;
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trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Plantae/Lathyrus_sativus_suspension_Linnaeus_> ;
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trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2022.113296> ;
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a trt:Treatment .
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<http://dx.doi.org/10.1016/j.phytochem.2022.113296>
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bibo:endPage "13" ;
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bibo:journal "Phytochemistry" ;
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bibo:pubDate "2022-10-31" ;
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bibo:series "113296" ;
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bibo:startPage "1" ;
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bibo:volume "202" ;
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dc:creator "Rathi, Divya; Verma, Jitendra Kumar; Chakraborty, Subhra; Chakraborty, Niranjan" ;
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dc:date "2022" ;
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dc:title "Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.8234551>, <http://dx.doi.org/10.5281/zenodo.8234553>, <http://dx.doi.org/10.5281/zenodo.8234555>, <http://dx.doi.org/10.5281/zenodo.8234557>, <http://dx.doi.org/10.5281/zenodo.8234559>, <http://dx.doi.org/10.5281/zenodo.8234561> ;
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a fabio:JournalArticle .
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<http://taxon-concept.plazi.org/id/Plantae/Lathyrus_sativus_suspension_Linnaeus_>
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dwc:authority "L." ;
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dwc:authorityName "L." ;
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dwc:class "Magnoliopsida" ;
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dwc:family "Fabaceae" ;
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dwc:genus "Lathyrus" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Fabales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "subSpecies" ;
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dwc:scientificNameAuthorship "Linnaeus" ;
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dwc:species "sativus" ;
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dwc:subSpecies "suspension" ;
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trt:hasTaxonName <http://taxon-name.plazi.org/id/Plantae/Lathyrus_sativus_suspension> ;
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a dwcFP:TaxonConcept .
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<http://taxon-name.plazi.org/id/Plantae/Lathyrus_sativus_suspension>
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dwc:class "Magnoliopsida" ;
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dwc:family "Fabaceae" ;
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dwc:genus "Lathyrus" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Fabales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "subSpecies" ;
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dwc:species "sativus" ;
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dwc:subSpecies "suspension" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Lathyrus_sativus> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Lathyrus_sativus>
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dwc:rank "species" ;
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dwc:species "sativus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Lathyrus> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Lathyrus>
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dwc:genus "Lathyrus" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Fabaceae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Fabaceae>
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dwc:family "Fabaceae" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Fabales> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Fabales>
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dwc:order "Fabales" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Magnoliopsida> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Magnoliopsida>
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dwc:class "Magnoliopsida" ;
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dwc:rank "class" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Tracheophyta> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Tracheophyta>
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dwc:phylum "Tracheophyta" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae>
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dwc:kingdom "Plantae" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.8234551>
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dc:description "Fig. 1. Schematic representation of the experimental design and workflow of the establishment of the grasspea suspension secretome (GSS). Proteomic profiling was accomplished by generating suspension culture and sequential assessment of physicochemical properties and protein identification." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234551/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234553>
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dc:description "Fig. 2. Generation of grasspea calli, establishment of suspension culture and isolation of the grasspea suspension secretome (GSS). (A) Root-cut and shoot-cut embryo axes were employed for the generation of 4-week-old calli, which were bulked together in a suspension culture. (B) Microscopic examination of suspension cells and viability assessment using Evans blue (left panel) and FDA (right panel). (C) Quantitative analysis of physicochemical properties including changes in pH in the suspension culture, fresh weight (FW), dry weight (DW), soluble sugars and total protein. (D) Protein SDS-PAGE profile of the grasspea secretome. Lane 1 represents the molecular weight marker (MW). Purity evaluation of grasspea secreted fraction using (E) catalase activity and (F) western blotting with anti-RbcL (Supplementary Fig. S1). Relative catalase activities are presented as mean ± SE of triplicate experiments." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234553/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234555>
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dc:description "Fig. 3. Overview of total grasspea suspension secreted (GSS) proteins and prediction of mode of secretion and (A) localization using multiple tools (B). Comparison of shared and distinct GSS proteins, first (C) with respect to total in vitro secretome (IVS) and in planta secretome (IPS) and second (D) compared to the in vitro suspension culture secretome reported in monocots, dicots, and lower plants, abbreviated as MSS, DSS and LSS, respectively (MSS corresponds to monocot suspension secretome, DSS to dicot suspension secretome and LSS to lower plant suspension secretome)." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234555/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234557>
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dc:description "Fig. 4. Physicochemical assessment of the grasspea suspension secretome (GSS), including pI (A), molecular weight (in kDa) (B), and hydrophilicity (C), with respect to MSS, DSS and LSS (MSS corresponds to the monocot suspension secretome, DSS to the dicot suspension secretome and LSS to the lower plant suspension secretome)." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234557/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234559>
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dc:description "Fig. 5. Functional annotation of the grasspea suspension secretome. (A) GO classification of proteins reveals the abundance of proteins associated with (B) response to abiotic stress, (C) response to biotic stress and (D) phytohormone signaling." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234559/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.8234561>
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dc:description "Fig. 6. Localization validation of endochitinase (S597) and G-type lectin S-receptor-like serine threonine kinase (S718). The panels include (A) expression of YFPtagged S597 in onion epidermal cells, (B) plasmolysis of S597-transformed onion peel (C), expression of YFP-tagged S718 in onion peel cells and (D) plasmolyzed onion peel cells expressing YFP-tagged S718. A pSITE3CA empty vector control was also monitored besides the target genes (E)." ;
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fabio:hasRepresentation <https://zenodo.org/record/8234561/files/figure.png> ;
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a fabio:Figure .
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