119 lines
6.8 KiB
Turtle
119 lines
6.8 KiB
Turtle
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
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@prefix cito: <http://purl.org/spar/cito/> .
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@prefix dc: <http://purl.org/dc/elements/1.1/> .
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@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
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@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
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@prefix fabio: <http://purl.org/spar/fabio/> .
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@prefix trt: <http://plazi.org/vocab/treatment#> .
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@prefix xlink: <http://www.w3.org/1999/xlink/> .
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<http://treatment.plazi.org/id/AC68879FFFB7BD1CFFE045FA3423782E>
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cito:cites <http://dx.doi.org/10.5281/zenodo.10483108>, <http://dx.doi.org/10.5281/zenodo.10483104> ;
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dc:creator "Rea, Kevin A; Casaretto, José A.; Al-Abdul-Wahid, M. Sameer; Sukumaran, Arjun; Geddes-McAlister, Jennifer; Rothstein, Steven J.; Akhtar, Tariq A." ;
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dc:title "Cannabis sativa" ;
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trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Plantae/Cannabis_sativa_McPartland_2001> ;
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trt:publishedIn <http://dx.doi.org/10.6084/m9.figshare.24741938.v1> ;
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a trt:Treatment .
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<http://dx.doi.org/10.6084/m9.figshare.24741938.v1>
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bibo:endPage "171" ;
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bibo:journal "Phytochemistry" ;
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bibo:pubDate "2019-05-28" ;
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bibo:startPage "162" ;
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bibo:volume "164" ;
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dc:creator "Rea, Kevin A; Casaretto, José A.; Al-Abdul-Wahid, M. Sameer; Sukumaran, Arjun; Geddes-McAlister, Jennifer; Rothstein, Steven J.; Akhtar, Tariq A." ;
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dc:date "2019" ;
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dc:title "Biosynthesis of cannflavins A and B from Cannabis sativa L" ;
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fabio:hasPart <http://dx.doi.org/10.5281/zenodo.10483108>, <http://dx.doi.org/10.5281/zenodo.10483104> ;
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a fabio:JournalArticle .
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<http://taxon-concept.plazi.org/id/Plantae/Cannabis_sativa_McPartland_2001>
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dwc:baseAuthorityName "McPartland and Russo" ;
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dwc:baseAuthorityYear "2001" ;
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dwc:class "Magnoliopsida" ;
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dwc:family "Cannabaceae" ;
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dwc:genus "Cannabis" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Rosales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "species" ;
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dwc:scientificNameAuthorship "(McPartland & Russo, 2001)" ;
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dwc:species "sativa" ;
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rdfs:seeAlso <https://www.catalogueoflife.org/data/taxon/QM84> ;
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trt:hasTaxonName <http://taxon-name.plazi.org/id/Plantae/Cannabis_sativa> ;
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a dwcFP:TaxonConcept .
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<http://taxon-name.plazi.org/id/Plantae/Cannabis_sativa>
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dwc:class "Magnoliopsida" ;
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dwc:family "Cannabaceae" ;
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dwc:genus "Cannabis" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Rosales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "species" ;
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dwc:species "sativa" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Cannabis> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Cannabis>
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dwc:class "Magnoliopsida" ;
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dwc:family "Cannabaceae" ;
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dwc:genus "Cannabis" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Rosales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "genus" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Cannabaceae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Cannabaceae>
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dwc:class "Magnoliopsida" ;
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dwc:family "Cannabaceae" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Rosales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "family" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Rosales> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Rosales>
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dwc:class "Magnoliopsida" ;
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dwc:kingdom "Plantae" ;
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dwc:order "Rosales" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "order" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Magnoliopsida> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Magnoliopsida>
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dwc:class "Magnoliopsida" ;
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dwc:kingdom "Plantae" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "class" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Tracheophyta> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae/Tracheophyta>
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dwc:kingdom "Plantae" ;
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dwc:phylum "Tracheophyta" ;
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dwc:rank "phylum" ;
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trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
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a dwcFP:TaxonName .
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<http://taxon-name.plazi.org/id/Plantae>
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dwc:kingdom "Plantae" ;
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dwc:rank "kingdom" ;
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a dwcFP:TaxonName .
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<http://dx.doi.org/10.5281/zenodo.10483108>
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dc:description "Figure 5. Enzymatic characterization of CsOMT21. (A) Three flavones (apigenin, luteolin and chrysoeriol) and two flavonols (quercetin and kaempferol) that typically accumulate in C. sativa were tested as potential substrates. (B) Relative enzyme activity and substrate preference. Recombinant CsOMT21 was purified by Ni2+-affinity chromatography (inset). Proteins were resolved by SDS-PAGE and stained with Coomassie Blue. Lanes 1 and 2 contain crude E. coli extracts containing CsOMT21 and the purified protein, respectively; Molecular weights (kDa) are indicated. The selected flavones and flavonols (as numbered above) were provided to recombinant CsOMT21 in enzyme assays along with [14C]-AdoMet as a methyl donor. Data are the means ± SD from three independent experiments and are presented as relative activity compared to that observed with the preferred substrate, luteolin. (C) Evidence for the conversion of luteolin to chrysoeriol by CsOMT21. Enzyme assays with CsOMT21 together with luteolin and unlabelled AdoMet as cosubstrates were extracted and analyzed by HPLC. Note the identical retention time and CID-Q-TOF mass spectral fragmentation pattern of the enzymatic product from these assays (bottom panel) with that of an authentic chrysoeriol standard (top panel). (D) Kinetic analysis of CsOMT21. Recombinant CsOMT21 was assayed under standard assay conditions at the indicated concentrations of luteolin. Kinetic parameters were determined by non-linear regression analysis using the Michaelis-Menten kinetics model of the SigmaPlot 12.3 software." ;
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fabio:hasRepresentation <https://zenodo.org/record/10483108/files/figure.png> ;
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a fabio:Figure .
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<http://dx.doi.org/10.5281/zenodo.10483104>
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dc:description "Fig. 4. Phylogenetic analysis of type 1 O-methyltransferases from C. sativa. A neighbour-joining phylogenetic tree (1000 replicates) of type 1 O-methyltransferases from diverse plants was constructed using the MEGA 6.0 software package. Included in this analysis are twenty-four unique type 1 O-methyltransferases (CsOMTs) that were identified in the C. sativa genome. Branch lengths indicate the number of amino acid substitutions per sequence and bootstrap values (> 60%) are indicated next to each branch. The amino acid sequences and species abbreviations for all of these type 1 o-methyltransferases are listed in Supplemental Fig. S1 along with their corresponding GenBank accession numbers. Note that the CsOMTs (bolded) are uniformly distributed amongst four main groups of plant type 1 proteins." ;
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fabio:hasRepresentation <https://zenodo.org/record/10483104/files/figure.png> ;
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a fabio:Figure .
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