@prefix rdf: . @prefix rdfs: . @prefix bibo: . @prefix cito: . @prefix dc: . @prefix dwc: . @prefix dwcFP: . @prefix fabio: . @prefix trt: . @prefix xlink: . cito:cites , , , , , , , , , , , ; dc:creator "Itagaki, Hiyori; Hosoya, Tsuyoshi" ; dc:title "Neobelonopsis Itagaki & Hosoya 2023, gen. nov." ; trt:definesTaxonConcept ; trt:publishedIn ; a trt:Treatment . bibo:endPage "85" ; bibo:journal "MycoKeys" ; bibo:pubDate "2023-08-14" ; bibo:startPage "45" ; bibo:volume "99" ; dc:creator "Itagaki, Hiyori; Hosoya, Tsuyoshi" ; dc:date "2023" ; dc:title "A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan" ; fabio:hasPart , , , , , , , , , , , ; a fabio:JournalArticle . dwc:LSID "977958E8-0407-518E-A7A3-9A6130E4F002" ; dwc:authority "Itagaki & Hosoya" ; dwc:authorityName "Itagaki & Hosoya" ; dwc:authorityYear "2023" ; dwc:family "Mollisiaceae" ; dwc:genus "Neobelonopsis" ; dwc:kingdom "Fungi" ; dwc:order "Helotiales" ; dwc:rank "genus" ; dwc:scientificNameAuthorship "Itagaki & Hosoya, 2023" ; trt:hasTaxonName ; a dwcFP:TaxonConcept . dwc:family "Mollisiaceae" ; dwc:genus "Neobelonopsis" ; dwc:kingdom "Fungi" ; dwc:order "Helotiales" ; dwc:rank "genus" ; trt:hasParentName ; a dwcFP:TaxonName . dwc:family "Mollisiaceae" ; dwc:kingdom "Fungi" ; dwc:order "Helotiales" ; dwc:rank "family" ; trt:hasParentName ; a dwcFP:TaxonName . dwc:kingdom "Fungi" ; dwc:order "Helotiales" ; dwc:rank "order" ; trt:hasParentName ; a dwcFP:TaxonName . dwc:kingdom "Fungi" ; dwc:rank "kingdom" ; a dwcFP:TaxonName . dc:description "Figure 3. Neobelonopsis acutata (TNS-F- 86648, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia C stromata with sparse subiculum D one month old colony on PDA E vertical section of the apothecium (in LA) F refractive vacuoles in fresh marginal cells (in water) G asci with ascospores (in CB / LA) H paraphyses (in CB / LA) I paraphyses with a long refractive vacuole (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L ascospores (in CB / LA) M conidia (in water) N, O conidiophores (in water) P bulbile (in CB / LA) Q subicular hyphae (in CB / LA). Scale bars: 1 mm (A, B); 0.2 mm (C); 25 μm (E, Q); 10 μm (F-P)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 4. Neobelonopsis bicolor (TNS-F- 86605, holotype) A fresh apothecia on decaying unidentified wood (TNS-F- 86666) B dried apothecia with yellowish disc and blackish flask (TNS-F- 86666) C dried apothecia with greyish discs D vertical section of the apothecium (TNS-F- 86666, in LA) E hyaline medullary excipulum (TNS-F- 86666, in LA) F brown medullary excipulum G ascospores (in CB / LA) H blue-stained apical pore of asci (in Melzer's solution after 3 % KOH pretreatment) I croziers at the base of asci (in CB / LA) J asci with ascospores (in CB / LA) K paraphyses (in CB / LA) L one month old colony on PDA M dark, gelatinous hyphal mass on CMA N monilioid hyphae surrounded by a gelatinous sheath (in diluted black ink) O octahedron crystals with monilioid hyphae on CMA (in water) P monilioid hyphae containing abundant guttles (in water). Scale bars: 1 mm (A, M); 0.5 mm (B, C); 50 μm (D-F); 25 μm (N, O); 10 μm (G-K, P)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 5. Neobelonopsis cinnabarina (TNS-F- 86682, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B immature apothecium protruding from the stroma C dried apothecia D one month old colony on PDA E texture of stroma (in LA) F vertical section of the apothecium (in LA) G yellowish reflective vacuoles in fresh paraphyses (in water) H yellowish vacuoles in outermost cells of fresh ectal excipulum (in water) I immature ascus with yellowish cytoplasm (in water) J ascospores with yellowish cytoplasm (in water) K apothecium that dissolved magenta pigments when immersed in 3 % KOH L outermost cells that turned magenta (in 3 % KOH) M fresh paraphyses that turned magenta, note that the tips have a darker color (in 3 % KOH) N ascospores (in CB / LA) O asci with ascospores (in CB / LA) P paraphyses with wide, blunt head (in CB / LA) Q blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) R crozier at the base of ascus (in CB / LA); S collapse conidiophores with slimy conidial drops on CMA (arrows) T hyphae with oblong-shape crystals on CMA U conidia (in water) V clusters of conidiophores (in water) W, X discrete conidiophores (in water). Scale bars: 1 mm (A, S); 0.1 mm (B); 0.5 mm (C); 50 μm (F-H, M, T, V); 20 μm (E); 10 μm (I, J, L, N-R, U, W, X)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 6. Neobelonopsis didymospora (TNS-F- 13501, holotype) A fresh apothecia on decaying Bamboo culm B dried apothecia on decaying Bamboo culm C fresh apothecia on decaying unidentified wood (TNS-F- 86670) D dried apothecia on decaying unidentified wood (TNS-F- 86670) E vertical section of the apothecium (in LA) F reflective vacuoles in fresh marginal cells (in water) G vertical section at the basal apothecium (in lactic acid) H ascospores (in CB / LA) I asci with ascospores (in CB / LA) J paraphyses (in CB / LA) K fresh paraphyses with long refractive vacuoles (in water) L blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) M crozier at the base of ascus (in CB / LA) N three months old colony on PDA O clusters of conidiophores (in CB / LA) P-S conidiophores with conidia (in CB / LA). Scale bars: 1 mm (A-D); 50 μm (E); 20 μm (O), 10 μm (F-M, P-S)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 7. Neobelonopsis microspora (TNS-F- 18068, holotype) A dried apothecia on the decaying culm of Sasa sp. B immature apothecia protruding from the stromata (arrows) C vertical section of the apothecium (in LA) D ascospores (in CB / LA) E asci with ascospores (in CB / LA) F paraphyses (in CB / LA) G blue-stained apical pore of asci (in Melzer's solution after 3 % KOH pretreatment) H, I croziers at the base of asci (in CB / LA) J conidiophores with pigmented collarets K conidiophores. Scale bars: 0.5 mm (A, B); 50 μm (C); 10 μm (D-K)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 8. Neobelonopsis multiguttata (TNS-F- 86402, holotype) A fresh apothecia on decaying culm of Sasa kurilensis B dried apothecia C stromata with sparse subiculum D vertical section of apothecium (in LA) E ascospores (in CB / LA) F asci with ascospores (in CB / LA) G paraphyses (in CB / LA) H blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) I croziers at the base of asci (in CB / LA) J texture of stroma (in LA) K subicular hyphae (in lactic acid) L three months old colony on PDA M conidia (in CB / LA) N-R conidiophores, arrows in N indicated conidiogenous cells (in CB / LA). Scale bars: 0.5 mm (A-C); 50 μm (D, K); 20 μm (N), 10 μm (E-J, M, O-R)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 9. Neobelonopsis obtusa (TNS-F- 15602, holotype) A fresh apothecia on decaying unidentified wood B fresh apothecia during moist conditions C dried apothecia D vertical section of the apothecium (in LA) E refractive vacuoles in fresh marginal cells (in water) F vertical section at the basal apothecium (in LA) G ascospores (in CB / LA) H asci with ascospores (in CB / LA) I paraphyses (in CB / LA) J fresh paraphyses with long refractive vacuoles (in water) K blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) L croziers at the base of asci (in CB / LA) M three months old colony on PDA N conidiophores with conidia (in water). Scale bars: 1 mm (A, C); 0.5 mm (B); 50 μm (D); 10 μm (E-L, N)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 10. Neobelonopsis ramosa (TNS-F- 86030, holotype) A dried apothecia on the decaying culm of Sasa sp. B stromata with sparse subiculum C vertical section of the apothecium (in LA) D asci (in CB / LA) E paraphyses (in CB / LA) F ascospores (in CB / LA) G blue-stained apical pore of asci (in Melzer's solution after 3 % KOH pretreatment) H croziers at the base of asci (in CB / LA) I subicular hyphae (in LA) J texture of stroma (in LA) K three months old colony on PDA. Scale bars: 0.5 mm (A); 0.25 mm (B); 50 μm (C, I); 20 μm (J); 10 μm (D-H)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 11. Trichobelonium albobarbatum (TNS-F- 86430, holotype) A fresh apothecia on the decaying culm of unidentified grass (Poaceae) B dried apothecium with hyaline connective hyphae C protruding immature apothecia from the stromata D vertical section of the apothecium (in LA) E crystals in medullary excipulum (in LA) F connecting hyphae between ectal excipulum and subiculum (in LA) G texture of the stroma (in LA) H ascospores (in CB / LA) I ascus with mature ascospores (in CB / LA) J ascus with immature ascospores (in CB / LA) K immature ascus containing yellowish oil globes in the cytoplasm (in CB / LA) L paraphysis (in CB / LA) M rehydrated paraphysis with fragmented refractive vacuoles (in water) N blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) O croziers at the base of asci (in CB / LA) P three months old colony on PDA Q yellow crystals formed on hyphae R acicular crystals (in water) S-V conidiophores with conidia (in CB / LA); W hyphae with exudates (in CB / LA). Scale bars: 0.5 mm (A, Q); 0.25 mm (B, C); 50 μm (D-F); 20 μm (R); 10 μm (G, H-O, S-W)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 13. Line-drawings of the Neobelonopsis and Trichobelonium species I vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium II asci III paraphyses IV ascospores. Scale bars: 10 μm." ; fabio:hasRepresentation ; a fabio:Figure . dc:description "Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." ; fabio:hasRepresentation ; a fabio:Figure .