@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix cito: <http://purl.org/spar/cito/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix trt: <http://plazi.org/vocab/treatment#> .
@prefix xlink: <http://www.w3.org/1999/xlink/> .

<http://treatment.plazi.org/id/713F878BFF91FFAA4768F949FEA6FE44>
    cito:cites <http://dx.doi.org/10.5281/zenodo.10483483>, <http://dx.doi.org/10.5281/zenodo.10483485> ;
    dc:creator "Papaefthimiou, Dimitra; Diretto, Gianfranco; Demurtas, Olivia Costantina; Mini, Paola; Ferrante, Paola; Giuliano, Giovanni; Kanellis, Angelos K." ;
    dc:title "Chlamydomonas chloroplast" ;
    trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Plantae/Chlamydomonas_Ehrenberg_1833> ;
    trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2019.112082> ;
    a trt:Treatment .

<http://dx.doi.org/10.1016/j.phytochem.2019.112082>
    bibo:endPage "8" ;
    bibo:journal "Phytochemistry" ;
    bibo:pubDate "2019-11-30" ;
    bibo:startPage "1" ;
    bibo:volume "167" ;
    dc:creator "Papaefthimiou, Dimitra; Diretto, Gianfranco; Demurtas, Olivia Costantina; Mini, Paola; Ferrante, Paola; Giuliano, Giovanni; Kanellis, Angelos K." ;
    dc:date "2019" ;
    dc:title "Heterologous production of labdane-type diterpenes in the green alga Chlamydomonas reinhardtii" ;
    fabio:hasPart <http://dx.doi.org/10.5281/zenodo.10483483>, <http://dx.doi.org/10.5281/zenodo.10483485> ;
    a fabio:JournalArticle .

<http://taxon-concept.plazi.org/id/Plantae/Chlamydomonas_Ehrenberg_1833>
    dwc:authority "Ehrenberg, 1833" ;
    dwc:class "Chlorophyceae" ;
    dwc:family "Chlamydomonadaceae" ;
    dwc:genus "Chlamydomonas" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Chlamydomonadales" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "genus" ;
    dwc:scientificNameAuthorship "Ehrenberg, 1833" ;
    rdfs:seeAlso <https://www.catalogueoflife.org/data/taxon/3NGJ> ;
    trt:hasTaxonName <http://taxon-name.plazi.org/id/Plantae/Chlamydomonas> ;
    a dwcFP:TaxonConcept .

<http://taxon-name.plazi.org/id/Plantae/Chlamydomonas>
    dwc:class "Chlorophyceae" ;
    dwc:family "Chlamydomonadaceae" ;
    dwc:genus "Chlamydomonas" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Chlamydomonadales" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "genus" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Chlamydomonadaceae> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Chlamydomonadaceae>
    dwc:class "Chlorophyceae" ;
    dwc:family "Chlamydomonadaceae" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Chlamydomonadales" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "family" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Chlamydomonadales> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Chlamydomonadales>
    dwc:class "Chlorophyceae" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Chlamydomonadales" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "order" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Chlorophyceae> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Chlorophyceae>
    dwc:class "Chlorophyceae" ;
    dwc:kingdom "Plantae" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "class" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Chlorophyta> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Chlorophyta>
    dwc:kingdom "Plantae" ;
    dwc:phylum "Chlorophyta" ;
    dwc:rank "phylum" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae>
    dwc:kingdom "Plantae" ;
    dwc:rank "kingdom" ;
    a dwcFP:TaxonName .

<http://dx.doi.org/10.5281/zenodo.10483483>
    dc:description "Fig. 1. Proposed pathway of labdane-type diterpenes predominant in Cistus creticus L. resin. A protonation-initiated cyclization, catalyzed by CcCLS, converts GGDP to the stable bicyclic intermediate copal-8-ol diphosphate. The formation of labda-13-ene-8α,15-diol, ent-manoyl oxide, ent-13-epi manoyl oxide and sclareol are probably due to the activity of non-specific terpenoid phosphatases. Abbreviations: CcCLS, Cistus creticus copal-8-ol diphosphate synthase." ;
    fabio:hasRepresentation <https://zenodo.org/record/10483483/files/figure.png> ;
    a fabio:Figure .

<http://dx.doi.org/10.5281/zenodo.10483485>
    dc:description "Fig. 2. Chloroplast transformation of C. reinhardtii with recombinant CO-CcCLS gene, and verification of transgene integration and homoplasmy. A. Schematic map of pCG2-FLAG-CO-CcCLS vector used for chloroplast transformation. The length of each genetic element is indicated in each box. atpA prom.: Promoter of the α- subunit of the chloroplast ATP synthase; aadA: CDS of spectinomycin resistance gene; rbcL Ter.: ribulose bisphosphate carboxylase large chain Terminator; psbA Ter.: Photosystem II protein D1 Terminator; CO-CcCLS: Codon adapted CLS gene; FLAG: Flag Tag; psbD prom.: Photosystem II protein D2 promoter. B. Integration scheme of pCG2-FLAG-CO-CcCLS plasmid in the chloroplast genome. Primers Chla1-for/Chla1-rev (amplicon 1: length 1358 bp) and Chla2-for/Chla2-rev (amplicon2: length 2519 bp) were used to test correct integration in the chloroplast genome while primers Chla3-for/Chla3-rev were used to check homoplasmy (amplicon 3: length 954 bp). The absence of amplicon 3 indicates that the transformants are homoplasmic for the presence of transgene. C. PCR results of seventeen representative transformants. Transformants indicated in red have correct plasmid integration and are homoplasmic. M: 1 kb DNA ladder (NEB, catalog number N3232S)." ;
    fabio:hasRepresentation <https://zenodo.org/record/10483485/files/figure.png> ;
    a fabio:Figure .