@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix cito: <http://purl.org/spar/cito/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix trt: <http://plazi.org/vocab/treatment#> .

<http://treatment.plazi.org/id/0396878CFFF1FFE0FFD4FA2DFCCCFEE9>
    cito:cites <http://dx.doi.org/10.5281/zenodo.10481853> ;
    dc:creator "Mohanraj, Soundappan S.; Gujjarlapudi, Mariyamma; Lokya, Vadthya; Mallikarjuna, Nalini; Dutta-Gupta, Aparna; Padmasree, Kollipara" ;
    dc:title "Rhynchosia sublobata subsp. protease Meikle" ;
    trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Plantae/Rhynchosia_sublobata_protease_Schumach_> ;
    trt:publishedIn <http://dx.doi.org/10.1016/j.phytochem.2018.12.018> ;
    a trt:Treatment .

<http://dx.doi.org/10.1016/j.phytochem.2018.12.018>
    bibo:endPage "171" ;
    bibo:journal "Phytochemistry" ;
    bibo:pubDate "2019-03-31" ;
    bibo:startPage "159" ;
    bibo:volume "159" ;
    dc:creator "Mohanraj, Soundappan S.; Gujjarlapudi, Mariyamma; Lokya, Vadthya; Mallikarjuna, Nalini; Dutta-Gupta, Aparna; Padmasree, Kollipara" ;
    dc:date "2019" ;
    dc:title "Purification and characterization of Bowman-Birk and Kunitz isoinhibitors from the seeds of Rhynchosia sublobata (Schumach.) Meikle, a wild relative of pigeonpea" ;
    fabio:hasPart <http://dx.doi.org/10.5281/zenodo.10481853> ;
    a fabio:JournalArticle .

<http://taxon-concept.plazi.org/id/Plantae/Rhynchosia_sublobata_protease_Schumach_>
    dwc:authority "(Schumach.) Meikle" ;
    dwc:authorityName "Meikle" ;
    dwc:baseAuthorityName "Schumach." ;
    dwc:class "Magnoliopsida" ;
    dwc:family "Fabaceae" ;
    dwc:genus "Rhynchosia" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Fabales" ;
    dwc:phylum "Tracheophyta" ;
    dwc:rank "subSpecies" ;
    dwc:scientificNameAuthorship "(Schumach.) Meikle" ;
    dwc:species "sublobata" ;
    dwc:subSpecies "protease" ;
    trt:hasTaxonName <http://taxon-name.plazi.org/id/Plantae/Rhynchosia_sublobata_protease> ;
    a dwcFP:TaxonConcept .

<http://taxon-name.plazi.org/id/Plantae/Rhynchosia_sublobata_protease>
    dwc:class "Magnoliopsida" ;
    dwc:family "Fabaceae" ;
    dwc:genus "Rhynchosia" ;
    dwc:kingdom "Plantae" ;
    dwc:order "Fabales" ;
    dwc:phylum "Tracheophyta" ;
    dwc:rank "subSpecies" ;
    dwc:species "sublobata" ;
    dwc:subSpecies "protease" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Rhynchosia_sublobata> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Rhynchosia_sublobata>
    dwc:rank "species" ;
    dwc:species "sublobata" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Rhynchosia> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Rhynchosia>
    dwc:genus "Rhynchosia" ;
    dwc:rank "genus" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Fabaceae> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Fabaceae>
    dwc:family "Fabaceae" ;
    dwc:rank "family" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Fabales> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Fabales>
    dwc:order "Fabales" ;
    dwc:rank "order" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Magnoliopsida> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Magnoliopsida>
    dwc:class "Magnoliopsida" ;
    dwc:rank "class" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae/Tracheophyta> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae/Tracheophyta>
    dwc:phylum "Tracheophyta" ;
    dwc:rank "phylum" ;
    trt:hasParentName <http://taxon-name.plazi.org/id/Plantae> ;
    a dwcFP:TaxonName .

<http://taxon-name.plazi.org/id/Plantae>
    dwc:kingdom "Plantae" ;
    dwc:rank "kingdom" ;
    a dwcFP:TaxonName .

<http://dx.doi.org/10.5281/zenodo.10481853>
    dc:description "Fig. 1. FPLC purification of ‘RsPI’ from R. sublobata seeds. Elution profiles of (A) trypsin-affinity and (B) gel filtration columns. The protein peaks active against trypsin were marked with an asterisk (*); (C) Tricine SDS-PAGE (15%) showing different fractions of purification protocol under non-reducing conditions. Lane 1 is loaded with Protein molecular weight marker (PMWM) and lanes 2–6 are loaded with 5 or 10 μg of corresponding protein sample. The gel was stained with silver nitrate. CPI – crude protease inhibitor, TAC – Trypsin affinity chromatography, GFC – Gel filtration chromatography and SBBI – Soybean BBI." ;
    fabio:hasRepresentation <https://zenodo.org/record/10481853/files/figure.png> ;
    a fabio:Figure .