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@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix cito: <http://purl.org/spar/cito/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix trt: <http://plazi.org/vocab/treatment#> .
<http://treatment.plazi.org/id/D06287D4FFB2FF9EFC1E05A3FE87FEE3>
cito:cites <http://dx.doi.org/10.5281/zenodo.14423119> ;
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:title "Paramecium tetraurelia" ;
trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Chromista/Paramecium_tetraurelia_Sonneborn_> ;
trt:publishedIn <https://doi.org/10.1093/zoolinnean/zlad196> ;
a trt:Treatment .
<https://doi.org/10.1093/zoolinnean/zlad196>
bibo:endPage "13" ;
bibo:issue "3" ;
bibo:journal "Zoological Journal of the Linnean Society" ;
bibo:pubDate "2024-02-08" ;
bibo:startPage "1" ;
bibo:volume "202" ;
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:date "2024" ;
dc:title "An overview of genetic manipulation tools in the studies of ciliates * with emphasis on gene knockout * knockdown * and overexpression" ;
fabio:hasPart <http://dx.doi.org/10.5281/zenodo.14423119> ;
a fabio:JournalArticle .
<http://taxon-concept.plazi.org/id/Chromista/Paramecium_tetraurelia_Sonneborn_>
dwc:authority "Sonneborn" ;
dwc:class "Hypotrichea" ;
dwc:family "Oxytrichidae" ;
dwc:genus "Paramecium" ;
dwc:kingdom "Chromista" ;
dwc:order "Oxytrichida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:scientificNameAuthorship "Sonneborn" ;
dwc:species "tetraurelia" ;
trt:hasTaxonName <http://taxon-name.plazi.org/id/Chromista/Paramecium_tetraurelia> ;
a dwcFP:TaxonConcept .
<http://taxon-name.plazi.org/id/Chromista/Paramecium_tetraurelia>
dwc:class "Hypotrichea" ;
dwc:family "Oxytrichidae" ;
dwc:genus "Paramecium" ;
dwc:kingdom "Chromista" ;
dwc:order "Oxytrichida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:species "tetraurelia" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Paramecium> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Paramecium>
dwc:genus "Paramecium" ;
dwc:rank "genus" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oxytrichidae> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oxytrichidae>
dwc:family "Oxytrichidae" ;
dwc:rank "family" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oxytrichida> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oxytrichida>
dwc:order "Oxytrichida" ;
dwc:rank "order" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Hypotrichea> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Hypotrichea>
dwc:class "Hypotrichea" ;
dwc:rank "class" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Ciliophora> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Ciliophora>
dwc:phylum "Ciliophora" ;
dwc:rank "phylum" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista>
dwc:kingdom "Chromista" ;
dwc:rank "kingdom" ;
a dwcFP:TaxonName .
<http://dx.doi.org/10.5281/zenodo.14423119>
dc:description "Figure 5. Gene overexpression strategies in ciliates.A* construct of gene overexpression and the homologous recombination in Tetrahymena (a) and positive transformed cell screening (b). Black arrows with coloured tails indicate the primers with 15–20 bp overlap designed for fusion PCR.Red arrows pointing to the plasmid indicate the restriction enzyme digestion sites. B* gene overexpression by injection of more copies in Paramecium. ºelomeres are indicated with red squares at the end of chromosomes. C* gene overexpression by introduction of artificial chromosomes in Oxytricha. Abbreviations:GOI* gene of interest; MAC* macronucleus; MIC* micronucleus; MT1 and MT3* cadmiuminducible metallothionein genes; neo Tet* a synthetic neo gene (neomycin-resistance gene) optimized for codon usage of Tetrahymena; PM* paromomycin." ;
fabio:hasRepresentation <https://zenodo.org/record/14423119/files/figure.png> ;
a fabio:Figure .

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@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix cito: <http://purl.org/spar/cito/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix trt: <http://plazi.org/vocab/treatment#> .
<http://treatment.plazi.org/id/D06287D4FFB3FF9EFEDF006CFD68F98D>
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:title "Oxytricha trifallax" ;
trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Chromista/Oxytricha_trifallax_al_1989> ;
trt:publishedIn <https://doi.org/10.1093/zoolinnean/zlad196> ;
a trt:Treatment .
<https://doi.org/10.1093/zoolinnean/zlad196>
bibo:endPage "13" ;
bibo:issue "3" ;
bibo:journal "Zoological Journal of the Linnean Society" ;
bibo:pubDate "2024-02-08" ;
bibo:startPage "1" ;
bibo:volume "202" ;
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:date "2024" ;
dc:title "An overview of genetic manipulation tools in the studies of ciliates * with emphasis on gene knockout * knockdown * and overexpression" ;
a fabio:JournalArticle .
<http://taxon-concept.plazi.org/id/Chromista/Oxytricha_trifallax_al_1989>
dwc:authority "Greslin et al., 1989" ;
dwc:class "Hypotrichea" ;
dwc:family "Oxytrichidae" ;
dwc:genus "Oxytricha" ;
dwc:kingdom "Chromista" ;
dwc:order "Oxytrichida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:scientificNameAuthorship "Greslin et al., 1989" ;
dwc:species "trifallax" ;
rdfs:seeAlso <https://www.catalogueoflife.org/data/taxon/75GSZ> ;
trt:hasTaxonName <http://taxon-name.plazi.org/id/Chromista/Oxytricha_trifallax> ;
a dwcFP:TaxonConcept .
<http://taxon-name.plazi.org/id/Chromista/Oxytricha_trifallax>
dwc:class "Hypotrichea" ;
dwc:family "Oxytrichidae" ;
dwc:genus "Oxytricha" ;
dwc:kingdom "Chromista" ;
dwc:order "Oxytrichida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:species "trifallax" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oxytricha> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oxytricha>
dwc:genus "Oxytricha" ;
dwc:rank "genus" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oxytrichidae> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oxytrichidae>
dwc:family "Oxytrichidae" ;
dwc:rank "family" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oxytrichida> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oxytrichida>
dwc:order "Oxytrichida" ;
dwc:rank "order" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Hypotrichea> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Hypotrichea>
dwc:class "Hypotrichea" ;
dwc:rank "class" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Ciliophora> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Ciliophora>
dwc:phylum "Ciliophora" ;
dwc:rank "phylum" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista>
dwc:kingdom "Chromista" ;
dwc:rank "kingdom" ;
a dwcFP:TaxonName .

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@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix cito: <http://purl.org/spar/cito/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix dwc: <http://rs.tdwg.org/dwc/terms/> .
@prefix dwcFP: <http://filteredpush.org/ontologies/oa/dwcFP#> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix trt: <http://plazi.org/vocab/treatment#> .
<http://treatment.plazi.org/id/D06287D4FFBDFF9FFC79077AFEA1F836>
cito:cites <http://dx.doi.org/10.5281/zenodo.14423119>, <http://dx.doi.org/10.5281/zenodo.14423111> ;
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:title "Tetrahymena thermophila" ;
trt:augmentsTaxonConcept <http://taxon-concept.plazi.org/id/Chromista/Tetrahymena_thermophila_Nanney_1976> ;
trt:publishedIn <https://doi.org/10.1093/zoolinnean/zlad196> ;
a trt:Treatment .
<https://doi.org/10.1093/zoolinnean/zlad196>
bibo:endPage "13" ;
bibo:issue "3" ;
bibo:journal "Zoological Journal of the Linnean Society" ;
bibo:pubDate "2024-02-08" ;
bibo:startPage "1" ;
bibo:volume "202" ;
dc:creator "Fu, Jinyu; Gao, Yunyi; Gao, Feng; Sheng, Yalan; Al-Farraj, Saleh A.; Chen, Zigui; Wang, Chundi" ;
dc:date "2024" ;
dc:title "An overview of genetic manipulation tools in the studies of ciliates * with emphasis on gene knockout * knockdown * and overexpression" ;
fabio:hasPart <http://dx.doi.org/10.5281/zenodo.14423119>, <http://dx.doi.org/10.5281/zenodo.14423111> ;
a fabio:JournalArticle .
<http://taxon-concept.plazi.org/id/Chromista/Tetrahymena_thermophila_Nanney_1976>
dwc:authority "Nanney & McCoy, 1976" ;
dwc:class "Oligohymenophorea" ;
dwc:family "Tetrahymenidae" ;
dwc:genus "Tetrahymena" ;
dwc:kingdom "Chromista" ;
dwc:order "Hymenostomatida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:scientificNameAuthorship "Nanney & McCoy, 1976" ;
dwc:species "thermophila" ;
trt:hasTaxonName <http://taxon-name.plazi.org/id/Chromista/Tetrahymena_thermophila> ;
a dwcFP:TaxonConcept .
<http://taxon-name.plazi.org/id/Chromista/Tetrahymena_thermophila>
dwc:class "Oligohymenophorea" ;
dwc:family "Tetrahymenidae" ;
dwc:genus "Tetrahymena" ;
dwc:kingdom "Chromista" ;
dwc:order "Hymenostomatida" ;
dwc:phylum "Ciliophora" ;
dwc:rank "species" ;
dwc:species "thermophila" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Tetrahymena> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Tetrahymena>
dwc:genus "Tetrahymena" ;
dwc:rank "genus" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Tetrahymenidae> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Tetrahymenidae>
dwc:family "Tetrahymenidae" ;
dwc:rank "family" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Hymenostomatida> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Hymenostomatida>
dwc:order "Hymenostomatida" ;
dwc:rank "order" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Oligohymenophorea> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Oligohymenophorea>
dwc:class "Oligohymenophorea" ;
dwc:rank "class" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista/Ciliophora> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista/Ciliophora>
dwc:phylum "Ciliophora" ;
dwc:rank "phylum" ;
trt:hasParentName <http://taxon-name.plazi.org/id/Chromista> ;
a dwcFP:TaxonName .
<http://taxon-name.plazi.org/id/Chromista>
dwc:kingdom "Chromista" ;
dwc:rank "kingdom" ;
a dwcFP:TaxonName .
<http://dx.doi.org/10.5281/zenodo.14423119>
dc:description "Figure 5. Gene overexpression strategies in ciliates.A* construct of gene overexpression and the homologous recombination in Tetrahymena (a) and positive transformed cell screening (b). Black arrows with coloured tails indicate the primers with 15–20 bp overlap designed for fusion PCR.Red arrows pointing to the plasmid indicate the restriction enzyme digestion sites. B* gene overexpression by injection of more copies in Paramecium. ºelomeres are indicated with red squares at the end of chromosomes. C* gene overexpression by introduction of artificial chromosomes in Oxytricha. Abbreviations:GOI* gene of interest; MAC* macronucleus; MIC* micronucleus; MT1 and MT3* cadmiuminducible metallothionein genes; neo Tet* a synthetic neo gene (neomycin-resistance gene) optimized for codon usage of Tetrahymena; PM* paromomycin." ;
fabio:hasRepresentation <https://zenodo.org/record/14423119/files/figure.png> ;
a fabio:Figure .
<http://dx.doi.org/10.5281/zenodo.14423111>
dc:description "Figure 2. Gene knockout based on homologous recombination in Tetrahymena thermophila. A* schematic diagram of neo4 selectable casseưe (a) and flow path of homologous recombination (b). Black arrows with coloured tails indicate the primers with 15–20 bp overlap designed for fusion PCR.Red arrows pointing at the plasmid indicate the restriction enzyme digestion sites. B* cell screening of macronuclear gene knockout. A cross covering the cell means that the cell is dead. Ŋe black bars represent the endogenous chromosomes* and pink rectangles indicate the exogenous DNA that has been integrated into the chromosomes. C* procedures for micronuclear gene knockout in Tetrahymena. Ŋe correspondence between genotypes and cell icons is listed in the key at the boưom less. Abbreviations:UºR* untranslated regions; BTU2* a gene encoding β-tubulin; GOI* gene of interest; MAC* macronucleus; MIC* micronucleus; 6-MP* 6-methylpurine; MT1* a cadmiuminducible metallothionein gene; neo Tet* a synthetic neo gene (neomycin-resistance gene) optimized for codon usage of Tetrahymena; PM* paromomycin." ;
fabio:hasRepresentation <https://zenodo.org/record/14423111/files/figure.png> ;
a fabio:Figure .