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cito: cites <http://dx.doi.org/10.5281/zenodo.10481853> ;
dc: creator " Mohanraj, Soundappan S.; Gujjarlapudi, Mariyamma; Lokya, Vadthya; Mallikarjuna, Nalini; Dutta-Gupta, Aparna; Padmasree, Kollipara " ;
dc: title " Rhynchosia sublobata subsp. protease Meikle " ;
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trt: publishedIn <http://dx.doi.org/10.1016/j.phytochem.2018.12.018> ;
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bibo: endPage " 171 " ;
bibo: journal " Phytochemistry " ;
bibo: pubDate " 2019-03-31 " ;
bibo: startPage " 159 " ;
bibo: volume " 159 " ;
dc: creator " Mohanraj, Soundappan S.; Gujjarlapudi, Mariyamma; Lokya, Vadthya; Mallikarjuna, Nalini; Dutta-Gupta, Aparna; Padmasree, Kollipara " ;
dc: date " 2019 " ;
dc: title " Purification and characterization of Bowman-Birk and Kunitz isoinhibitors from the seeds of Rhynchosia sublobata (Schumach.) Meikle, a wild relative of pigeonpea " ;
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dwc: authority " (Schumach.) Meikle " ;
dwc: authorityName " Meikle " ;
dwc: baseAuthorityName " Schumach. " ;
dwc: class " Magnoliopsida " ;
dwc: family " Fabaceae " ;
dwc: genus " Rhynchosia " ;
dwc: kingdom " Plantae " ;
dwc: order " Fabales " ;
dwc: phylum " Tracheophyta " ;
dwc: rank " subSpecies " ;
dwc: scientificNameAuthorship " (Schumach.) Meikle " ;
dwc: species " sublobata " ;
dwc: subSpecies " protease " ;
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dwc: rank " subSpecies " ;
dwc: species " sublobata " ;
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dc: description " Fig. 1. FPLC purification of ‘ RsPI’ from R. sublobata seeds. Elution profiles of (A) trypsin-affinity and (B) gel filtration columns. The protein peaks active against trypsin were marked with an asterisk (*); (C) Tricine SDS-PAGE (15%) showing different fractions of purification protocol under non-reducing conditions. Lane 1 is loaded with Protein molecular weight marker (PMWM) and lanes 2– 6 are loaded with 5 or 10 μg of corresponding protein sample. The gel was stained with silver nitrate. CPI – crude protease inhibitor, TAC – Trypsin affinity chromatography, GFC – Gel filtration chromatography and SBBI – Soybean BBI. " ;
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